Gene for peripheral arterial occlusive disease

ABSTRACT

A role of the human PAOD1 gene in peripheral arterial occlusive disease is disclosed. Methods for diagnosis, prediction of clinical course and treatment for peripheral arterial occlusive disease using polymorphisms in the PAOD1 gene are also disclosed.

RELATED APPLICATION

[0001] This application is a continuation-in-part which claims priorityto U.S. application Ser. No. 10/060,902, filed Jan. 30, 2002 (convertedto provisional application No. ______). The entire teachings of theabove applications are incorporated herein by reference.

BACKGROUND OF THE INVENTION

[0002] Atherosclerosis is the pathology underlying several of mankind'smost lethal diseases, such as myocardial infarction and peripheralarterial occlusive disease. PAOD shares the risk factors of otheratherosclerotic diseases, especially smoking, diabetes, hypertension andhyperlipidemia (Dormandy, J., et al., Semin. Vasc. Surg., 12:123 (1999);Hooi, J. D., et al., Br. J. Gen. Pract. 49:49 (1999)). PAOD representsatherosclerosis of the large and medium arteries of the limbs,particularly to the lower extremities and includes the aorta and iliacarteries. It often coexists with coronary artery disease andcerebrovascular disease. Clinically significant lesions may graduallynarrow the peripheral arteries leading to pain on walking usuallyrelieved by rest (claudication), ischemic ulcers, gangrene, andsometimes limb amputation. Medical therapy is generally ineffective butoperations bypassing or replacing the lesion with artificial or venousgrafts improve blood flow distally, at least until they becomerestenosed (Haustein, K. O., Int. J. Clin. Pharmacol. Ther., 35:266(1997)).

SUMMARY OF THE INVENTION

[0003] As described herein, it has been discovered that the gene(hereinafter referred to as “PAOD”) that encodes prostaglandin Ereceptor 3 (subtype EP3) (known as PTGER3 or EP but also referred toherein as a PAOD1 protein or polypeptide) has been correlated throughhuman linkage studies to peripheral arterial occlusive disease,particularly atherosclerosis of the limbs and lower extremities. Thepresent invention relates to isolated nucleic acid molecules comprisinga PAOD1 nucleic acid. In one embodiment, the isolated nucleic acidmolecule comprises a nucleotide sequence selected from the groupconsisting of SEQ ID NO: 1 which may optionally comprise at least onepolymorphism as shown in Table 1, and the complement thereof. Theinvention further relates to a nucleic acid molecule which hybridizesunder high stringency conditions to a nucleotide sequence selected fromthe group consisting of SEQ ID NO: 1 which may optionally comprise atleast one polymorphism as shown in Table 1, and the complement thereof.The invention additionally relates to isolated nucleic acid molecules(e.g., cDNA molecules) encoding a PAOD1 polypeptide (e.g., encodingisoforms such as those set forth in SEQ ID NOs: 16, 18, 20, 22, 24, 26,28, 30, 32, 34, 36, or another splicing variant of PAOD1 polypeptidewhich includes a polymorphic site and/or exon 4 or 5). Two new exonshave been identified (exons 4 and 5) and are shown in FIGS. 3 and 5A to5B. Two new splicing variants (isoforms) are described containing thenovel exons, referred to herein as EP_(3g) and EP_(3h) (see FIGS. 3 and6A to 6F).

[0004] The invention further provides a method for assaying a sample forthe presence of a nucleic acid molecule comprising all or a portion ofPAOD1 in a sample, comprising contacting said sample with a secondnucleic acid molecule comprising a nucleotide sequence encoding a PAOD1polypeptide (e.g., SEQ ID NO: 1 or the complement of SEQ ID NO: 1 whichmay optionally comprise at least one polymorphism as shown in Table 1; anucleotide sequence encoding an isoform or splicing variant such asthose selected from the group consisting of any one of SEQ ID NOs: 16,18, 20, 22, 24, 26, 28, 30, 32, 34, 36, which may optionally comprise atleast one polymorphism as shown in Table 1, or another splicing variantof PAOD1 polypeptide which includes a polymorphic site and/or exon 4 or5), or a fragment or derivative thereof, under conditions appropriatefor selective hybridization. The invention additionally provides amethod for assaying a sample for the level of expression of a PAOD1polypeptide, or fragment or derivative thereof, comprising detecting(directly or indirectly) the level of expression of the PAOD1polypeptide, fragment or derivative thereof.

[0005] The invention also relates to a vector comprising an isolatednucleic acid molecule of the invention operatively linked to aregulatory sequence, as well as to a recombinant host cell comprisingthe vector. The invention also provides a method for preparing apolypeptide encoded by an isolated nucleic acid molecule describedherein (an PAOD1 polypeptide), comprising culturing a recombinant hostcell of the invention under conditions suitable for expression of saidnucleic acid molecule.

[0006] The invention further provides an isolated polypeptide encoded byisolated nucleic acid molecules of the invention (e.g., PAOD1polypeptide), as well as fragments or derivatives thereof. In aparticular embodiment, the polypeptide comprises the amino acid sequenceof any one of SEQ ID NOs: 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36,and containing at least one polymorphism described herein. In anotherembodiment, the polypeptide is another splicing variant of an PAOD1polypeptide, particularly a splicing variant containing all or a portionof exon 4 and/or exon 5. The invention also relates to an isolatedpolypeptide comprising an amino acid sequence which is greater thanabout 90 percent identical to the amino acid sequence of any one of SEQID NOs: 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36 and containing atleast one polymorphism described herein, preferably about 95 percentidentical, and even more preferably about 98 percent identical.

[0007] The invention also relates to an antibody, or an antigen-bindingfragment thereof, which selectively binds to a polypeptide of theinvention, as well as to a method for assaying the presence of apolypeptide encoded by an isolated nucleic acid molecule of theinvention in a sample, comprising contacting said sample with anantibody which specifically binds to the encoded polypeptide.

[0008] The invention further relates to methods of diagnosing apredisposition to peripheral arterial occlusive disease. The methods ofdiagnosing a predisposition to peripheral arterial occlusive disease inan individual include detecting the presence of an alteration in PAOD1,as well as detecting alterations in expression of an PAOD1 polypeptide,such as the presence of different splicing variants of PAOD1polypeptides. The alterations in expression can be quantitative,qualitative, or both quantitative and qualitative. The methods of theinvention allow the accurate diagnosis of peripheral arterial occlusivedisease at or before disease onset, thus reducing or minimizing thedebilitating effects of peripheral arterial occlusive disease.

[0009] The invention additionally relates to an assay for identifyingagents which alter (e.g., enhance or inhibit) the activity or expressionof one or more PAOD1 polypeptides. For example, a cell, cellularfraction, or solution containing an PAOD1 polypeptide or a fragment orderivative thereof, can be contacted with an agent to be tested, and thelevel of PAOD1 polypeptide expression or activity can be assessed. Theactivity or expression of more than one PAOD1 polypeptides can beassessed concurrently (e.g., the cell, cellular fraction, or solutioncan contain more than one type of PAOD1 polypeptide, such as differentsplicing variants, and the levels of the different polypeptides orsplicing variants can be assessed).

[0010] In another embodiment, the invention relates to assays toidentify polypeptides which interact with one or more PAOD1polypeptides. In a yeast two-hybrid system, for example, a first vectoris used which includes a nucleic acid encoding a DNA binding domain andalso an PAOD1 polypeptide, splicing variant, or fragment or derivativethereof, and a second vector is used which includes a nucleic acidencoding a transcription activation domain and also a nucleic acidencoding a polypeptide which potentially may interact with the PAOD1polypeptide, splicing variant, or fragment or derivative thereof (e.g.,a PAOD1 polypeptide binding agent or receptor). Incubation of yeastcontaining both the first vector and the second vector under appropriateconditions allows identification of polypeptides which interact with thePAOD1 polypeptide or fragment or derivative thereof, and thus can beagents which alter the activity of expression of an PAOD1 polypeptide.

[0011] Agents that enhance or inhibit PAOD1 polypeptide expression oractivity are also included in the current invention, as are methods ofaltering (enhancing or inhibiting) PAOD1 polypeptide expression oractivity by contacting a cell containing PAOD1 and/or polypeptide, or bycontacting the PAOD1 polypeptide, with an agent that enhances orinhibits expression or activity of PAOD1 or polypeptide.

[0012] Additionally, the invention pertains to pharmaceuticalcompositions comprising the nucleic acids of the invention, thepolypeptides of the invention, and/or the agents that alter activity ofPAOD1 polypeptide. The invention further pertains to methods of treatingperipheral arterial occlusive disease, by administering PAOD1therapeutic agents, such as nucleic acids of the invention, polypeptidesof the invention, the agents that alter activity of PAOD1 polypeptide,or compositions comprising the nucleic acids, polypeptides, and/or theagents that alter activity of PAOD1 polypeptide.

BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The foregoing and other objects, features and advantages of theinvention will be apparent from the following more particulardescription of preferred embodiments of the invention, as illustrated inthe accompanying drawings.

[0014] FIGS. 1A-1C show three families used in the linkage study ofPAOD1. Two of the families, A and B, have positive lod scores at thechromosome 1p31 locus, while the cousin pair in family C contributesnegatively. Sex indicators have been shuffled for some individuals inthe top two generations, and unaffected siblings of patients are notshown, to protect privacy. The darkened squares and circles representmen and women, respectively, affected with PAOD1. The cross-hatchedshading represents a PAOD1 patient who also had stroke. The slashedsymbols represent deceased individuals.

[0015]FIG. 2 shows multipoint allele-sharing lod score of chromosome 1with extra microsatellite markers within PAOD11. The solid linerepresents the results of all 272 PAOD1 patients. The dashed linerepresents the results of defining affecteds as PAOD1 without stroke.

[0016]FIG. 3 shows various isoforms of PAOD1, including new isoform EP3gand EP3h. Known variants have been reported. See Schmid A, Thierauch KH, Schleuning W D, Dinter H. Splice variants of the human EP3 receptorfor prostaglandin E2, Eur. J. Biochem., 15;228(1):23-30, February 1995;Kotani et al., Genomics, 40:425-434(1997).

[0017]FIGS. 4.1 to 4.83 show the annotated genomic nucleic acid sequenceof PAOD1 (SEQ ID NO: 1). The ORF starts at ATG at nts 58,162. For exon,the open reading frame starts at ATG and ends at GT. The 5′UTR isupstream of the ATG. For exons 2 and 3, the GT is an internal splicesite used in all isoforms except EP3c. The first nts of the 3′UTR forisoform c are underlined. For exon 4, may have splice sites at either AGor AA. For exon 6, in the a2 isoform of EPa1 and a2, exon 6 is cut atthe GT and spliced to a 3′UTR that begins at 2,563 nts; underlined inboth cases are the first nts of the 3′UTR. Exon 9 is part of twoisoforms called EP3v and EP3vi (Kotani, M. et al., Genomics 40:425-434(1997). See Table 4.

[0018]FIGS. 5A to 5B show the nucleic acid (Exons 1 and 2) sequences ofexons 1 through 12 (SEQ ID NOs: 2-14).

[0019]FIGS. 6A to 6F show nucleic acid and amino acid sequences for thePTGER3 isoforms (SEQ ID NOs: 15-36). Exon 2 is indicated by the firstunderline; both are found in all isoforms and code for most of theprotein, i.e., the 359 amino acids that make up the extracellular domainand all 7 trans-membrane spanning helices. Tail-forming exons (indicatedin bold) make up the different PTGER3 isoforms. The beginning of the 3′UTR is indicated by the second underling.

DETAILED DESCRIPTION OF THE INVENTION

[0020] Extensive genealogical information for a population withpopulation-based lists of patients has been combined with powerfulgenome sharing methods to map the first major locus in common peripheralarterial occlusive disease. A genome wide scan on patients, relatedwithin 6 meiotic events, diagnosed with peripheral arterial occlusivedisease and their unaffected relatives has been completed. Locus PAOD11on chromosome 1p31 has been identified through linkage studies to beassociated with peripheral arterial occlusive disease. This locus doesnot correspond to known susceptibility loci for peripheral arterialocclusive disease or its risk factors (such as diabetes, hyperlipidemiaand hypertension), and represents the first mapping of a gene for commonperipheral arterial occlusive disease. Until now there have been noknown linkage studies of peripheral arterial occlusive disease in humansshowing any connection to this region of the chromosome. Based on thelinkage studies conducted, Applicant has discovered a directrelationship between the PAOD1 gene and peripheral arterial occlusivedisease. Although the PAOD1 gene (i.e., cDNA but not the genomicsequence) from normal individuals is known, there have been no studiesdirectly investigating PAOD1 and peripheral arterial occlusive disease.Moreover, there have been no variant forms reported that have beenassociated with peripheral arterial occlusive disease. The full sequenceof the PAOD1 gene and splicing variants are shown in the Figures and SEQID NO: 1. Additional single nucleotide polymorphisms are reported inTable 1 but may not be shown in SEQ ID NO: 1. It should be understoodthat the nucleic acids and their gene products embraced by the inventioninclude the nucleotide sequence set forth in SEQ ID NO: 1 and mayfurther comprise at least one polymorphism as shown in Table 1.

[0021] Nucleic Acids of the Invention

[0022] Nucleic Acids, Portions and Variants

[0023] Accordingly, the invention pertains to an isolated nucleic acidmolecules comprising human PAOD1 nucleic acids having at least onenucleotide alteration and correlated with incidence of peripheralarterial occlusive disease. The term, “PAOD1 or variant PAOD1,” as usedherein, refers to an isolated nucleic acid molecule on chromosome 1p31that is associated with a susceptibility to a number of peripheralarterial occlusive disease phenotypes, and also to a portion or fragmentof the isolated nucleic acid molecule (e.g., cDNA or the gene) thatencodes PAOD1 polypeptide (e.g., the polypeptide having any one of SEQID NOs: 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, as shown in theFigures and optionally comprising at least one SNP as set forth in Table1, or another splicing variant of a PAOD1 polypeptide). In a preferredembodiment, the isolated nucleic acid molecule comprises SEQ ID NO: 1(shown in FIG. 4) or the complement thereof. In another embodiment, theisolated nucleic acid molecule comprises the sequence of SEQ ID NO: 1 orthe complement of SEQ ID NO: 1, except that one or more singlenucleotide polymorphisms as shown in Table 1 are also present. Inanother embodiment, the isolated nucleic acid molecules comprises exon 4or 5, or both exon 4 and 5.

[0024] The isolated nucleic acid molecules of the present invention canbe RNA, for example, mRNA, or DNA, such as cDNA and genomic DNA. DNAmolecules can be double-stranded or single-stranded; single stranded RNAor DNA can be either the coding, or sense, strand or the non-coding, orantisense, strand. The nucleic acid molecule can include all or aportion of the coding sequence of the gene and can further compriseadditional non-coding sequences such as introns and non-coding 3′ and 5′sequences (including regulatory sequences, for example). Additionally,the nucleic acid molecule can be fused to a marker sequence, forexample, a sequence that encodes a polypeptide to assist in isolation orpurification of the polypeptide. Such sequences include, but are notlimited to, those which encode a glutathione-S-transferase (GST) fusionprotein and those which encode a hemagglutinin A (HA) polypeptide markerfrom influenza.

[0025] An “isolated” nucleic acid molecule, as used herein, is one thatis separated from nucleic acids which normally flank the gene ornucleotide sequence (as in genomic sequences) and/or has been completelyor partially purified from other transcribed sequences (e.g., as in anRNA library). For example, an isolated nucleic acid of the invention maybe substantially isolated with respect to the complex cellular milieu inwhich it naturally occurs, or culture medium when produced byrecombinant techniques, or chemical precursors or other chemicals whenchemically synthesized. In some instances, the isolated material willform part of a composition (for example, a crude extract containingother substances), buffer system or reagent mix. In other circumstances,the material may be purified to essential homogeneity, for example asdetermined by PAGE or column chromatography such as HPLC. Preferably, anisolated nucleic acid molecule comprises at least about 50, 80 or 90%(on a molar basis) of all macromolecular species present. With regard togenomic DNA, the term “isolated” also can refer to nucleic acidmolecules which are separated from the chromosome with which the genomicDNA is naturally associated. For example, the isolated nucleic acidmolecule can contain less than about 5 kb, 4 kb, 3 kb, 2 kb, 1 kb, 0.5kb or 0.1 kb of nucleotides which flank the nucleic acid molecule in thegenomic DNA of the cell from which the nucleic acid molecule is derived.

[0026] The nucleic acid molecule can be fused to other coding orregulatory sequences and still be considered isolated. Thus, recombinantDNA contained in a vector is included in the definition of “isolated” asused herein. Also, isolated nucleic acid molecules include recombinantDNA molecules in heterologous host cells, as well as partially orsubstantially purified DNA molecules in solution. “Isolated” nucleicacid molecules also encompass in vivo and in vitro RNA transcripts ofthe DNA molecules of the present invention. An isolated nucleic acidmolecule or nucleotide sequence can include a nucleic acid molecule ornucleotide sequence which is synthesized chemically or by recombinantmeans. Therefore, recombinant DNA contained in a vector are included inthe definition of “isolated” as used herein. Also, isolated nucleotidesequences include recombinant DNA molecules in heterologous organisms,as well as partially or substantially purified DNA molecules insolution. In vivo and in vitro RNA transcripts of the DNA molecules ofthe present invention are also encompassed by “isolated” nucleotidesequences. Such isolated nucleotide sequences are useful in themanufacture of the encoded polypeptide, as probes for isolatinghomologous sequences (e.g., from other mammalian species), for genemapping (e.g., by in situ hybridization with chromosomes), or fordetecting expression of the gene in tissue (e.g., human tissue), such asby Northern blot analysis.

[0027] The present invention also pertains to variant nucleic acidmolecules which are not necessarily found in nature but which encode aPAOD1 polypeptide (e.g., a polypeptide having the amino acid sequence ofany one of SEQ ID NOs: 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36 oranother isoform or splicing variant of PAOD1 polypeptide or polymorphicvariant thereof). Thus, for example, DNA molecules which comprise asequence that is different from the naturally-occurring nucleotidesequence but which, due to the degeneracy of the genetic code, encode aPAOD1 polypeptide of the present invention are also the subject of thisinvention. The invention also encompasses nucleotide sequences encodingportions (fragments), or encoding variant polypeptides such as analoguesor derivatives of the PAOD1 polypeptide. Such variants can benaturally-occurring, such as in the case of allelic variation or singlenucleotide polymorphisms, or non-naturally-occurring, such as thoseinduced by various mutagens and mutagenic processes. Intended variationsinclude, but are not limited to, addition, deletion and substitution ofone or more nucleotides which can result in conservative ornon-conservative amino acid changes, including additions and deletions.Preferably the nucleotide (and/or resultant amino acid) changes aresilent or conserved; that is, they do not alter the characteristics oractivity of the PAOD1 polypeptide. In one preferred embodiment, thenucleotide sequences are fragments that comprise one or more polymorphicmicrosatellite markers. In another preferred embodiment, the nucleotidesequences are fragments that comprise one or more single nucleotidepolymorphisms in the PAOD1 gene.

[0028] Other alterations of the nucleic acid molecules of the inventioncan include, for example, labeling, methylation, internucleotidemodifications such as uncharged linkages (e.g., methyl phosphonates,phosphotriesters, phosphoamidates, carbamates), charged linkages (e.g.,phosphorothioates, phosphorodithioates), pendent moieties (e.g.,polypeptides), intercalators (e.g., acridine, psoralen), chelators,alkylators, and modified linkages (e.g., alpha anomeric nucleic acids).Also included are synthetic molecules that mimic nucleic acid moleculesin the ability to bind to a designated sequences via hydrogen bondingand other chemical interactions. Such molecules include, for example,those in which peptide linkages substitute for phosphate linkages in thebackbone of the molecule.

[0029] The invention also pertains to nucleic acid molecules whichhybridize under high stringency hybridization conditions, such as forselective hybridization, to a nucleotide sequence described herein(e.g., nucleic acid molecules which specifically hybridize to anucleotide sequence encoding polypeptides described herein, and,optionally, have an activity of the polypeptide). In one embodiment, theinvention includes variants described herein which hybridize under highstringency hybridization conditions (e.g., for selective hybridization)to a nucleotide sequence comprising a nucleotide sequence selected fromSEQ ID NO: 1 which may optionally comprise at least one polymorphism asshown in Table 1 or the complement thereof. In another embodiment, theinvention includes variants described herein which hybridize under highstringency hybridization conditions (e.g., for selective hybridization)to a nucleotide sequence encoding an amino acid sequence selected fromany one of SEQ ID NOs: 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, orother polymorphic variant thereof. In a preferred embodiment, thevariant which hybridizes under high stringency hybridizations has anactivity of PAOD1.

[0030] Such nucleic acid molecules can be detected and/or isolated byspecific hybridization (e.g., under high stringency conditions).“Specific hybridization,” as used herein, refers to the ability of afirst nucleic acid to hybridize to a second nucleic acid in a mannersuch that the first nucleic acid does not hybridize to any nucleic acidother than to the second nucleic acid (e.g., when the first nucleic acidhas a higher similarity to the second nucleic acid than to any othernucleic acid in a sample wherein the hybridization is to-be performed).“Stringency conditions” for hybridization is a term of art which refersto the incubation and wash conditions, e.g., conditions of temperatureand buffer concentration, which permit hybridization of a particularnucleic acid to a second nucleic acid; the first nucleic acid may beperfectly (i.e., 100%) complementary to the second, or the first andsecond may share some degree of complementarity which is less thanperfect (e.g., 70%, 75%, 85%, 90%, 95%, 98%). For example, certain highstringency conditions can be used which distinguish perfectlycomplementary nucleic acids from those of less complementarity. “Highstringency conditions”, “moderate stringency conditions” and “lowstringency conditions” for nucleic acid hybridizations are explained onpages 2.10.1-2.10.16 and pages 6.3.1-6.3.6 in Current Protocols inMolecular Biology (Ausubel, F. M. et al., “Current Protocols inMolecular Biology”, John Wiley & Sons, (1998), the entire teachings ofwhich are incorporated by reference herein). The exact conditions whichdetermine the stringency of hybridization depend not only on ionicstrength (e.g., 0.2×SSC, 0.1×SSC), temperature (e.g., room temperature,42° C., 68° C.) and the concentration of destabilizing agents such asformamide or denaturing agents such as SDS, but also on factors such asthe length of the nucleic acid sequence, base composition, percentmismatch between hybridizing sequences and the frequency of occurrenceof subsets of that sequence within other non-identical sequences. Thus,equivalent conditions can be determined by varying one or more of theseparameters while maintaining a similar degree of identity or similaritybetween the two nucleic acid molecules. Typically, conditions are usedsuch that sequences at least about 60%, at least about 70%, at leastabout 80%, at least about 90% or at least about 95% or more identical toeach other remain hybridized to one another. By varying hybridizationconditions from a level of stringency at which no hybridization occursto a level at which hybridization is first observed, conditions whichwill allow a given sequence to hybridize (e.g., selectively) with themost similar sequences in the sample can be determined.

[0031] Exemplary conditions are described in Krause, M. H. and S. A.Aaronson, Methods in Enzymology, 200:546-556 (1991). Also, in, Ausubel,et al., “Current Protocols in Molecular Biology”, John Wiley & Sons,(1998), which describes the determination of washing conditions formoderate or low stringency conditions. Washing is the step in whichconditions are usually set so as to determine a minimum level ofcomplementarity of the hybrids. Generally, starting from the lowesttemperature at which only homologous hybridization occurs, each ° C. bywhich the final wash temperature is reduced (holding SSC concentrationconstant) allows an increase by 1% in the maximum extent of mismatchingamong the sequences that hybridize. Generally, doubling theconcentration of SSC results in an increase in T_(m) of ˜17° C. Usingthese guidelines, the washing temperature can be determined empiricallyfor high, moderate or low stringency, depending on the level of mismatchsought.

[0032] For example, a low stringency wash can comprise washing in asolution containing 0.2×SSC/0.1% SDS for 10 min at room temperature; amoderate stringency wash can comprise washing in a prewarmed solution(42° C.) solution containing 0.2×SSC/0.1% SDS for 15 min at 42° C.; anda high stringency wash can comprise washing in prewarmed (68° C.)solution containing 0.1×SSC/0.1%SDS for 15 min at 68° C. Furthermore,washes can be performed repeatedly or sequentially to obtain a desiredresult as known in the art. Equivalent conditions can be determined byvarying one or more of the parameters given as an example, as known inthe art, while maintaining a similar degree of identity or similaritybetween the target nucleic acid molecule and the primer or probe used.

[0033] The percent homology or identity of two nucleotide or amino acidsequences can be determined by aligning the sequences for optimalcomparison purposes (e.g., gaps can be introduced in the sequence of afirst sequence for optimal alignment). The nucleotides or amino acids atcorresponding positions are then compared, and the percent identitybetween the two sequences is a function of the number of identicalpositions shared by the sequences (i.e., % identity=# of identicalpositions/total # of positions×100). When a position in one sequence isoccupied by the same nucleotide or amino acid residue as thecorresponding position in the other sequence, then the molecules arehomologous at that position. As used herein, nucleic acid or amino acid“homology” is equivalent to nucleic acid or amino acid “identity”. Incertain embodiments, the length of a sequence aligned for comparisonpurposes is at least 30%, for example, at least 40%, in certainembodiments at least 60%, and in other embodiments at least 70%, 80%,90% or 95% of the length of the reference sequence. The actualcomparison of the two sequences can be accomplished by well-knownmethods, for example, using a mathematical algorithm. A preferred,non-limiting example of such a mathematical algorithm is described inKarlin et al., Proc. Natl. Acad. Sci. USA 90:5873-5877 (1993). Such analgorithm is incorporated into the NBLAST and XBLAST programs (version2.0) as described in Altschul et al., Nucleic Acids Res. 25:389-3402(1997). When utilizing BLAST and Gapped BLAST programs, the defaultparameters of the respective programs (e.g., NBLAST) can be used. In oneembodiment, parameters for sequence comparison can be set at score=100,wordlength=12, or can be varied (e.g., W=5 or W=20).

[0034] Another preferred, non-limiting example of a mathematicalalgorithm utilized for the comparison of sequences is the algorithm ofMyers and Miller, CABIOS (1989). Such an algorithm is incorporated intothe ALIGN program (version 2.0) which is part of the GCG-sequencealignment software package (Acceirys, Cambridge, UK). When utilizing theALIGN program for comparing amino acid sequences, a PAM120 weightresidue table, a gap length penalty of 12, and a gap penalty of 4 can beused. Additional algorithms for sequence analysis are known in the artand include ADVANCE and ADAM as described in Torellis and Robotti (1994)Comput. Appl. Biosci., 10:3-5; and FASTA described in Pearson and Lipman(1988) PNAS, 85:2444-8.

[0035] In another embodiment, the percent identity between two aminoacid sequences can be accomplished using the GAP program in theGCG-software package using either a Blossom 63 matrix or a PAM250matrix, and a gap weight of 12, 10, 8, 6, or 4 and a length weight of 2,3, or 4. In yet another embodiment, the percent identity between twonucleic acid sequences can be accomplished using the GAP program in theGCG-software package, using a gap weight of 50 and a length weight of 3.

[0036] The present invention also provides isolated nucleic acidmolecules that contain a fragment or portion that hybridizes underhighly stringent conditions to a nucleotide sequence comprising anucleotide sequence selected from SEQ ID NO: 1 which may optionallycomprise at least one polymorphism as shown in Table 1 and thecomplement thereof, and also provides isolated nucleic acid moleculesthat contain a fragment or portion that hybridizes under highlystringent conditions to a nucleotide sequence encoding an amino acidsequence selected from any one of SEQ ID NOs: 16, 18, 20, 22, 24, 26,28, 30, 32, 34, 36, or polymorphic variant thereof. The nucleic acidfragments of the invention are at least about 15, preferably at leastabout 18, 20, 23 or 25 nucleotides, and can be 30, 40, 50, 100, 200 ormore nucleotides in length. Longer fragments, for example, 30 or morenucleotides in length, which encode antigenic polypeptides describedherein are particularly useful, such as for the generation of antibodiesas described below.

[0037] Probes and Primers

[0038] In a related aspect, the nucleic acid fragments of the inventionare used as probes or primers in assays such as those described herein.“Probes” or “primers” are oligonucleotides that hybridize in abase-specific manner to a complementary strand of nucleic acidmolecules. Such probes and primers include polypeptide nucleic acids, asdescribed in Nielsen et al., Science, 254, 1497-1500 (1991).

[0039] A probe or primer comprises a region of nucleotide sequence thathybridizes to at least about 15, for example about 20-25, and in certainembodiments about 40, 50 or 75, consecutive nucleotides of a nucleicacid molecule comprising a contiguous nucleotide sequence or polymorphicvariant thereof. In other embodiments, a probe or primer comprises 100or fewer nucleotides, in certain embodiments from 6 to 50 nucleotides,for example from 12 to 30 nucleotides. In other embodiments, the probeor primer is at least 70% identical to the contiguous nucleotidesequence or to the complement of the contiguous nucleotide sequence, forexample at least 80% identical, in certain embodiments at least 90%identical, and in other embodiments at least 95% identical, or evencapable of selectively hybridizing to the contiguous nucleotide sequenceor to the complement of the contiguous nucleotide sequence. Often, theprobe or primer further comprises a label, e.g., radioisotope,fluorescent compound, enzyme, or enzyme co-factor.

[0040] The nucleic acid molecules of the invention such as thosedescribed above can be identified and isolated using standard molecularbiology techniques and the sequence information provided herein. Forexample, nucleic acid molecules can be amplified and isolated by thepolymerase chain reaction using synthetic oligonucleotide primersdesigned based on one or more of the sequences provided in SEQ ID NO: 1which may optionally comprise at least one polymorphism shown in Table1, and/or the complement thereof, or designed based on nucleotides basedon sequences encoding one or more of the amino acid sequences providedherein. See generally PCR Technology: Principles and Applications forDNA Amplification (ed. H. A. Erlich, Freeman Press, NY, N.Y., 1992); PCRProtocols. A Guide to Methods and Applications (Eds. Innis, et al.,Academic Press, San Diego, Calif., 1990); Mattila et al., Nucleic AcidsRes., 19:4967 (1991); Eckert et al., PCR Methods and Applications, 1:17(1991); PCR (eds. McPherson et al., IRL Press, Oxford); and U.S. Pat.No. 4,683,202. The nucleic acid molecules can be amplified using cDNA,mRNA or genomic DNA as a template, cloned into an appropriate vector andcharacterized by DNA sequence analysis.

[0041] Other suitable amplification methods include the ligase chainreaction (LCR) (see Wu and Wallace, Genomics, 4:560 (1989), Landegren etal., Science, 241:1077 (1988), transcription amplification (Kwoh et al,Proc. Natl. Acad. Sci. USA, 86:1173 (1989)), and self-sustained sequencereplication (Guatelli et al., Proc. Nat. Acad. Sci. USA, 87:1874 (1990))and nucleic acid based sequence amplification (NASBA). The latter twoamplification methods involve isothermal reactions based on isothermaltranscription, which produce both single stranded RNA (ssRNA) and doublestranded DNA (dsDNA) as the amplification products in a ratio of about30 or 100 to 1, respectively.

[0042] The amplified DNA can be labelled, for example radiolabelled, andused as a probe for screening a cDNA library derived from human cells,mRNA in zap express, ZIPLOX or other suitable vector. Correspondingclones can be isolated, DNA can obtained following in vivo excision, andthe cloned insert can be sequenced in either or both orientations by artrecognized methods to identify the correct reading frame encoding apolypeptide of the appropriate molecular weight. For example, the directanalysis of the nucleotide sequence of nucleic acid molecules of thepresent invention can be accomplished using well-known methods that arecommercially available. See, for example, Sambrook et al., MolecularCloning, A Laboratory Manual (2nd Ed., CSHP, New York 1989); Zyskind etal., Recombinant DNA Laboratory Manual, (Acad. Press, 1988)). Usingthese or similar methods, the polypeptide and the DNA encoding thepolypeptide can be isolated, sequenced and further characterized.

[0043] Antisense nucleic acid molecules of the invention can be designedusing the nucleotide sequences of SEQ ID NO: 1 and/or the complement ofSEQ ID NO: 1, and/or a portion of SEQ ID NO: 1 or the complement of SEQID NO: 1 and/or a sequence encoding the amino acid sequences or any oneof SEQ ID NOs: 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or encoding aportion of any one of SEQ ID NOs: 16, 18, 20, 22, 24, 26, 28, 30, 32,34, 36, (wherein any one of these may optionally comprise at least onepolymorphism as shown in Table 1) and constructed using chemicalsynthesis and enzymatic ligation reactions using procedures known in theart. For example, an antisense nucleic acid molecule (e.g., an antisenseoligonucleotide) can be chemically synthesized using naturally occurringnucleotides or variously modified nucleotides designed to increase thebiological stability of the molecules or to increase the physicalstability of the duplex formed between the antisense and sense nucleicacids, e.g., phosphorothioate derivatives and acridine substitutednucleotides can be used. Alternatively, the antisense nucleic acidmolecule can be produced biologically using an expression vector intowhich a nucleic acid molecule has been subcloned in an antisenseorientation (i.e., RNA transcribed from the inserted nucleic acidmolecule will be of an antisense orientation to a target nucleic acid ofinterest).

[0044] In general, the isolated nucleic acid sequences of the inventioncan be used as molecular weight markers on Southern gels, and aschromosome markers which are labeled to map related gene positions. Thenucleic acid sequences can also be used to compare with endogenous DNAsequences in patients to identify genetic disorders (e.g., apredisposition for or susceptibility to peripheral arterial occlusivedisease), and as probes, such as to hybridize and discover related DNAsequences or to subtract out known sequences from a sample. The nucleicacid sequences can further be used to derive primers for geneticfingerprinting, to raise anti-polypeptide antibodies using DNAimmunization techniques, and as an antigen to raise anti-DNA antibodiesor elicit immune responses. Portions or fragments of the nucleotidesequences identified herein (and the corresponding complete genesequences) can be used in numerous ways as polynucleotide reagents. Forexample, these sequences can be used to: (i) map their respective geneson a chromosome; and, thus, locate gene regions associated with geneticdisease; (ii) identify an individual from a minute biological sample(tissue typing); and (iii) aid in forensic identification of abiological sample. Additionally, the nucleotide sequences of theinvention can be used to identify and express recombinant polypeptidesfor analysis, characterization or therapeutic use, or as markers fortissues in which the corresponding polypeptide is expressed, eitherconstitutively, during tissue differentiation, or in diseased states.The nucleic acid sequences can additionally be used as reagents in thescreening and/or diagnostic assays described herein, and can also beincluded as components of kits (e.g., reagent kits) for use in thescreening and/or diagnostic assays described herein.

[0045] Vectors

[0046] Another aspect of the invention pertains to nucleic acidconstructs containing a nucleic acid molecule selected from the groupconsisting of SEQ ID NO: 1 which may optionally comprise at least onepolymorphism shown in Table 1 and the complement thereof (or a portionthereof). Yet another aspect of the invention pertains to nucleic acidconstructs containing a nucleic acid molecule encoding the amino acidsequence of any one of SEQ ID NOs: 16, 18, 20, 22, 24, 26, 28, 30, 32,34, 36, or polymorphic variant thereof. The constructs comprise a vector(e.g., an expression vector) into which a sequence of the invention hasbeen inserted in a sense or antisense orientation. As used herein, theterm “vector” refers to a nucleic acid molecule capable of transportinganother nucleic acid to which it has been linked. One type of vector isa “plasmid”, which refers to a circular double stranded DNA loop intowhich additional DNA segments can be ligated. Another type of vector isa viral vector, wherein additional DNA segments can be ligated into theviral genome. Certain vectors are capable of autonomous replication in ahost cell into which they are introduced (e.g., bacterial vectors havinga bacterial origin of replication and episomal mammalian vectors). Othervectors (e.g., non-episomal mammalian vectors) are integrated into thegenome of a host cell upon introduction into the host cell, and therebyare replicated along with the host genome. Moreover, certain vectors,expression vectors, are capable of directing the expression of genes towhich they are operably linked. In general, expression vectors ofutility in recombinant DNA techniques are often in the form of plasmids.However, the invention is intended to include such other forms ofexpression vectors, such as viral vectors (e.g., replication defectiveretroviruses, adenoviruses and adeno-associated viruses) that serveequivalent functions.

[0047] Preferred recombinant expression vectors of the inventioncomprise a nucleic acid molecule of the invention in a form suitable forexpression of the nucleic acid molecule in a host cell. This means thatthe recombinant expression vectors include one or more regulatorysequences, selected on the basis of the host cells to be used forexpression, which is operably linked to the nucleic acid sequence to beexpressed. Within a recombinant expression vector, “operably oroperatively linked” is intended to mean that the nucleotide sequence ofinterest is linked to the regulatory sequence(s) in a manner whichallows for expression of the nucleotide sequence (e.g., in an in vitrotranscription/translation system or in a host cell when the vector isintroduced into the host cell). The term “regulatory sequence” isintended to include promoters, enhancers and other expression controlelements (e.g., polyadenylation signals). Such regulatory sequences aredescribed, for example, in Goeddel, Gene Expression Technology: Methodsin Enzymology 185, Academic Press, San Diego, Calif. (1990). Regulatorysequences include those which direct constitutive expression of anucleotide sequence in many types of host cell and those which directexpression of the nucleotide sequence only in certain host cells (e.g.,tissue-specific regulatory sequences). It will be appreciated by thoseskilled in the art that the design of the expression vector can dependon such factors as the choice of the host cell to be transformed and thelevel of expression of polypeptide desired. The expression vectors ofthe invention can be introduced into host cells to thereby producepolypeptides, including fusion polypeptides, encoded by nucleic acidmolecules as described herein.

[0048] The recombinant expression vectors of the invention can bedesigned for expression of a polypeptide of the invention in prokaryoticor eukaryotic cells, e.g., bacterial cells such as E. coli, insect cells(using baculovirus expression vectors), yeast cells or mammalian cells.Suitable host cells are discussed further in Goeddel, supra.Alternatively, the recombinant expression vector can be transcribed andtranslated in vitro, for example using T7 promoter regulatory sequencesand T7 polymerase.

[0049] Another aspect of the invention pertains to host cells into whicha recombinant expression vector of the invention has been introduced.The terms “host cell” and “recombinant host cell” are usedinterchangeably herein. It is understood that such terms refer not onlyto the particular subject cell but also to the progeny or potentialprogeny of such a cell. Because certain modifications may occur insucceeding generations due to either mutation or environmentalinfluences, such progeny may not, in fact, be identical to the parentcell, but are still included within the scope of the term as usedherein.

[0050] A host cell can be any prokaryotic or eukaryotic cell. Forexample, a nucleic acid molecule of the invention can be expressed inbacterial cells (e.g., E. coli), insect cells, yeast or mammalian cells(such as Chinese hamster ovary cells (CHO) or COS cells). Other suitablehost cells are known to those skilled in the art.

[0051] Vector DNA can be introduced into prokaryotic or eukaryotic cellsvia conventional transformation or transfection techniques. As usedherein, the terms “transformation” and “transfection” are intended torefer to a variety of art-recognized techniques for introducing aforeign nucleic acid molecule (e.g., DNA) into a host cell, includingcalcium phosphate or calcium chloride co-precipitation,DEAE-dextran-mediated transfection, lipofection, or electroporation.Suitable methods for transforming or transfecting host cells can befound in Sambrook, et al. (supra), and other laboratory manuals.

[0052] For stable transfection of mammalian cells, it is known that,depending upon the expression vector and transfection technique used,only a small fraction of cells may integrate the foreign DNA into theirgenome. In order to identify and select these integrants, a gene thatencodes a selectable marker (e.g., for resistance to antibiotics) isgenerally introduced into the host cells along with the gene ofinterest. Preferred selectable markers include those that conferresistance to drugs, such as G418, hygromycin and methotrexate. Nucleicacid molecules encoding a selectable marker can be introduced into ahost cell on the same vector as the nucleic acid molecule of theinvention or can be introduced on a separate vector. Cells stablytransfected with the introduced nucleic acid molecule can be identifiedby drug selection (e.g., cells that have incorporated the selectablemarker gene will survive, while the other cells die).

[0053] A host cell of the invention, such as a prokaryotic or eukaryotichost cell in culture, can be used to produce (i.e., express) apolypeptide of the invention. Accordingly, the invention furtherprovides methods for producing a polypeptide using the host cells of theinvention. In one embodiment, the method comprises culturing the hostcell of invention (into which a recombinant expression vector encoding apolypeptide of the invention has been introduced) in a suitable mediumsuch that the polypeptide is produced. In another embodiment, the methodfurther comprises isolating the polypeptide from the medium or the hostcell.

[0054] The host cells of the invention can also be used to producenonhuman transgenic animals. For example, in one embodiment, a host cellof the invention is a fertilized oocyte or an embryonic stem cell intowhich a nucleic acid molecule of the invention has been introduced(e.g., an exogenous PAOD1 gene, or an exogenous nucleic acid encodingPAOD1 polypeptide). Such host cells can then be used to create non-humantransgenic animals in which exogenous nucleotide sequences have beenintroduced into the genome or homologous recombinant animals in whichendogenous nucleotide sequences have been altered. Such animals areuseful for studying the function and/or activity of the nucleotidesequence and polypeptide encoded by the sequence and for identifyingand/or evaluating modulators of their activity. As used herein, a“transgenic animal” is a non-human animal, preferably a mammal, morepreferably a rodent such as a rat or mouse, in which one or more of thecells of the animal includes a transgene. Other examples of transgenicanimals include non-human primates, sheep, dogs, cows, goats, chickensand amphibians. A transgene is exogenous DNA which is integrated intothe genome of a cell from which a transgenic animal develops and whichremains in the genome of the mature animal, thereby directing theexpression of an encoded gene product in one or more cell types ortissues of the transgenic animal. As used herein, an “homologousrecombinant animal” is a non-human animal, preferably a mammal, morepreferably a mouse, in which an endogenous gene has been altered byhomologous recombination between the endogenous gene and an exogenousDNA molecule introduced into a cell of the animal, e.g., an embryoniccell of the animal, prior to development of the animal.

[0055] Methods for generating transgenic animals via embryo manipulationand microinjection, particularly animals such as mice, have becomeconventional in the art and are described, for example, in U.S. Pat.Nos. 4,736,866 and 4,870,009, U.S. Pat. No. 4,873,191 and in Hogan,Manipulating the Mouse Embryo (Cold Spring Harbor Laboratory Press, ColdSpring Harbor, N.Y., 1986). Methods for constructing homologousrecombination vectors and homologous recombinant animals are describedfurther in Bradley (1991) Current Opinion in Bio/Technology, 2:823-829and in PCT Publication Nos. WO 90/11354, WO 91/01140, WO 92/0968, and WO93/04169. Clones of the non-human transgenic animals described hereincan also be produced according to the methods described in Wilmut et al.(1997) Nature, 385:810-813 and PCT Publication Nos. WO 97/07668 and WO97/07669.

[0056] Polypeptides of the Invention

[0057] The present invention also pertains to isolated polypeptidesencoded by PAOD1 (“PAOD1 polypeptides”) and fragments and variantsthereof, as well as polypeptides encoded by nucleotide sequencesdescribed herein (e.g., other splicing variants). The term “polypeptide”refers to a polymer of amino acids, and not to a specific length; thus,peptides, oligopeptides and proteins are included within the definitionof a polypeptide. As used herein, a polypeptide is said to be “isolated”or “purified” when it is substantially free of cellular material when itis isolated from recombinant and non-recombinant cells, or free ofchemical precursors or other chemicals when it is chemicallysynthesized. A polypeptide, however, can be joined to anotherpolypeptide with which it is not normally associated in a cell (e.g., ina “fusion protein”) and still be “isolated” or “purified.”

[0058] The polypeptides of the invention can be purified to homogeneity.It is understood, however, that preparations in which the polypeptide isnot purified to homogeneity are useful. The critical feature is that thepreparation allows for the desired function of the polypeptide, even inthe presence of considerable amounts of other components. Thus, theinvention encompasses various degrees of purity. In one embodiment, thelanguage “substantially free of cellular material” includes preparationsof the polypeptide having less than about 30% (by dry weight) otherproteins (i.e., contaminating protein), less than about 20% otherproteins, less than about 10% other proteins, or less than about 5%other proteins.

[0059] When a polypeptide is recombinantly produced, it can also besubstantially free of culture medium, i.e., culture medium representsless than about 20%, less than about 10%, or less than about 5% of thevolume of the polypeptide preparation. The language “substantially freeof chemical precursors or other chemicals” includes preparations of thepolypeptide in which it is separated from chemical precursors or otherchemicals that are involved in its synthesis. In one embodiment, thelanguage “substantially free of chemical precursors or other chemicals”includes preparations of the polypeptide having less than about 30% (bydry weight) chemical precursors or other chemicals, less than about 20%chemical precursors or other chemicals, less than about 10% chemicalprecursors or other chemicals, or less than about 5% chemical precursorsor other chemicals.

[0060] In one embodiment, a polypeptide of the invention comprises anamino acid sequence encoded by a nucleic acid molecule comprising anucleotide sequence selected from the group consisting of SEQ ID NO: 1which may optionally comprise at least one polymorphism shown in Table 1and complements and portions thereof, e.g., any one of SEQ ID NOs: 16,18, 20, 22, 24, 26, 28, 30, 32, 34, 36 or a portion or polymorphicvariant thereof. However, the polypeptides of the invention alsoencompass fragment and sequence variants. Variants include asubstantially homologous polypeptide encoded by the same genetic locusin an organism, i.e., an allelic variant, as well as other splicingvariants. Variants also encompass polypeptides derived from othergenetic loci in an organism, but having substantial homology to apolypeptide encoded by a nucleic acid molecule comprising a nucleotidesequence selected from the group consisting of SEQ ID NO: 1 which mayoptionally comprise at least one polymorphism shown in Table 1 andcomplements and portions thereof, or having substantial homology to apolypeptide encoded by a nucleic acid molecule comprising a nucleotidesequence selected from the group consisting of nucleotide sequencesencoding any one of SEQ ID NOs: 16, 18, 20, 22, 24, 26, 28, 30, 32, 34,36, or polymorphic variants thereof. Variants also include polypeptidessubstantially homologous or identical to these polypeptides but derivedfrom another organism, i.e., an ortholog. Variants also includepolypeptides that are substantially homologous or identical to thesepolypeptides that are produced by chemical synthesis. Variants alsoinclude polypeptides that are substantially homologous or identical tothese polypeptides that are produced by recombinant methods.

[0061] As used herein, two polypeptides (or a region of thepolypeptides) are substantially homologous or identical when the aminoacid sequences are at least about 45-55%, in certain embodiments atleast about 70-75%, in other embodiments at least about 80-85%, and inother embodiments greater than about 90% or more homologous oridentical. A substantially homologous amino acid sequence, according tothe present invention, will be encoded by a nucleic acid moleculehybridizing to SEQ ID NO: 1 which may optionally comprise at least onepolymorphism shown in Table 1, or portion thereof, under stringentconditions as more particularly described above, or will be encoded by anucleic acid molecule hybridizing to a nucleic acid sequence encodingany one of SEQ ID NOs: 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36,portion thereof or polymorphic variant thereof, under stringentconditions as more particularly described thereof.

[0062] The invention also encompasses polypeptides having a lower degreeof identity but having sufficient similarity so as to perform one ormore of the same functions performed by a polypeptide encoded by anucleic acid molecule of the invention. Similarity is determined byconserved amino acid substitution. Such substitutions are those thatsubstitute a given amino acid in a polypeptide by another amino acid oflike characteristics. Conservative substitutions are likely to bephenotypically silent. Typically seen as conservative substitutions arethe replacements, one for another, among the aliphatic amino acids Ala,Val, Leu and Ile; interchange of the hydroxyl residues Ser and Thr,exchange of the acidic residues Asp and Glu, substitution between theamide residues Asn and Gln, exchange of the basic residues Lys and Argand replacements among the aromatic residues Phe and Tyr. Guidanceconcerning which amino acid changes are likely to be phenotypicallysilent are found in Bowie et al., Science 247:1306-1310 (1990).

[0063] A variant polypeptide can differ in amino acid sequence by one ormore substitutions, deletions, insertions, inversions, fusions, andtruncations or a combination of any of these. Further, variantpolypeptides can be fully functional or can lack function in one or moreactivities. Fully functional variants typically contain onlyconservative variation or variation in non-critical residues or innon-critical regions. Functional variants can also contain substitutionof similar amino acids that result in no change or an insignificantchange in function. Alternatively, such substitutions may positively ornegatively affect function to some degree. Non-functional variantstypically contain one or more non-conservative amino acid substitutions,deletions, insertions, inversions, or truncation or a substitution,insertion, inversion, or deletion in a critical residue or criticalregion.

[0064] Amino acids that are essential for function can be identified bymethods known in the art, such as site-directed mutagenesis oralanine-scanning mutagenesis (Cunningham et al., Science, 244:1081-1085(1989)). The latter procedure introduces single alanine mutations atevery residue in the molecule. The resulting mutant molecules are thentested for biological activity in vitro, or in vitro proliferativeactivity. Sites that are critical for polypeptide activity can also bedetermined by structural analysis such as crystallization, nuclearmagnetic resonance or photoaffinity labeling (Smith et al., J. Mol.Biol., 224:899-904 (1992); de Vos et al., Science, 255:306-312 (1992)).

[0065] The invention also includes polypeptide fragments of thepolypeptides of the invention. Fragments can be derived from apolypeptide encoded by a nucleic acid molecule comprising SEQ ID NO: 1which may optionally comprise at least one polymorphism shown in Table 1or a portion thereof and the complements thereof (e.g., any one of SEQID NOs: 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or other splicingvariants). However, the invention also encompasses fragments of thevariants of the polypeptides described herein. As used herein, afragment comprises at least 6 contiguous amino acids. Useful fragmentsinclude those that retain one or more of the biological activities ofthe polypeptide as well as fragments that can be used as an immunogen togenerate polypeptide-specific antibodies.

[0066] Biologically active fragments (peptides which are, for example,6, 9, 12, 15, 16, 20, 30, 35, 36, 37, 38, 39, 40, 50, 100 or more aminoacids in length) can comprise a domain, segment, or motif that has beenidentified by analysis of the polypeptide sequence using well-knownmethods, e.g., signal peptides, extracellular domains, one or moretransmembrane segments or loops, ligand binding regions, zinc fingerdomains, DNA binding domains, acylation sites, glycosylation sites, orphosphorylation sites.

[0067] Fragments can be discrete (not fused to other amino acids orpolypeptides) or can be within a larger polypeptide. Further, severalfragments can be comprised within a single larger polypeptide. In oneembodiment a fragment designed for expression in a host can haveheterologous pre- and pro-polypeptide regions fused to the aminoterminus of the polypeptide fragment and an additional region fused tothe carboxyl terminus of the fragment.

[0068] The invention thus provides chimeric or fusion polypeptides.These comprise a polypeptide of the invention operatively linked to aheterologous protein or polypeptide having an amino acid sequence notsubstantially homologous to the polypeptide. “Operatively linked”indicates that the polypeptide and the heterologous protein are fusedin-frame. The heterologous protein can be fused to the N-terminus orC-terminus of the polypeptide. In one embodiment the fusion polypeptidedoes not affect function of the polypeptide per se. For example, thefusion polypeptide can be a GST-fusion polypeptide in which thepolypeptide sequences are fused to the C-terminus of the GST sequences.Other types of fusion polypeptides include, but are not limited to,enzymatic fusion polypeptides, for example β-galactosidase fusions,yeast two-hybrid GAL fusions, poly-His fusions and Ig fusions. Suchfusion polypeptides, particularly poly-His fusions, can facilitate thepurification of recombinant polypeptide. In certain host cells (e.g.,mammalian host cells), expression and/or secretion of a polypeptide canbe increased by using a heterologous signal sequence. Therefore, inanother embodiment, the fusion polypeptide contains a heterologoussignal sequence at its N-terminus.

[0069] EP-A-O 464 533 discloses fusion proteins comprising variousportions of immunoglobulin constant regions. The Fe is useful in therapyand diagnosis and thus results, for example, in improved pharmacokineticproperties (EP-A 0232 262). In drug discovery, for example, humanproteins have been fused with Fe portions for the purpose ofhigh-throughput screening assays to identify antagonists. Bennett etal., Journal of Molecular Recognition, 8:52-58 (1995) and Johanson etal., The Journal of Biological Chemistry, 270,16:9459-9471 (1995). Thus,this invention also encompasses soluble fusion polypeptides containing apolypeptide of the invention and various portions of the constantregions of heavy or light chains of immunoglobulins of various subclass(IgG, IgM, IgA, IgE).

[0070] A chimeric or fusion polypeptide can be produced by standardrecombinant DNA techniques. For example, DNA fragments coding for thedifferent polypeptide sequences are ligated together in-frame inaccordance with conventional techniques. In another embodiment, thefusion gene can be synthesized by conventional techniques includingautomated DNA synthesizers. Alternatively, PCR amplification of nucleicacid fragments can be carried out using anchor primers which give riseto complementary overhangs between two consecutive nucleic acidfragments which can subsequently be annealed and re-amplified togenerate a chimeric nucleic acid sequence (see Ausubel et al., CurrentProtocols in Molecular Biology, 1992). Moreover, many expression vectorsare commercially available that already encode a fusion moiety (e.g., aGST protein). A nucleic acid molecule encoding a polypeptide of theinvention can be cloned into such an expression vector such that thefusion moiety is linked in-frame to the polypeptide.

[0071] The isolated polypeptide can be purified from cells thatnaturally express it, purified from cells that have been altered toexpress it (recombinant), or synthesized using known protein synthesismethods. In one embodiment, the polypeptide is produced by recombinantDNA techniques. For example, a nucleic acid molecule encoding thepolypeptide is cloned into an expression vector, the expression vectorintroduced into a host cell and the polypeptide expressed in the hostcell. The polypeptide can then be isolated from the cells by anappropriate purification scheme using standard protein purificationtechniques.

[0072] In general, polypeptides of the present invention can be used asa molecular weight marker on SDS-PAGE gels or on molecular sieve gelfiltration columns using art-recognized methods. The polypeptides of thepresent invention can be used to raise antibodies or to elicit an immuneresponse. The polypeptides can also be used as a reagent, e.g., alabeled reagent, in assays to quantitatively determine levels of thepolypeptide or a molecule to which it binds (e.g., a receptor or aligand) in biological fluids. The polypeptides can also be used asmarkers for cells or tissues in which the corresponding polypeptide ispreferentially expressed, either constitutively, during tissuedifferentiation, or in a diseased state. The polypeptides can be used toisolate a corresponding binding agent, e.g., receptor or ligand, suchas, for example, in an interaction trap assay, and to screen for peptideor small molecule antagonists or agonists of the binding interaction.

[0073] Antibodies of the Invention

[0074] Polyclonal and/or monoclonal antibodies that specifically bindone form of the gene product but not to the other form of the geneproduct are also provided. Antibodies are also provided that bind aportion of either the variant or the reference gene product thatcontains the polymorphic site or sites. The invention providesantibodies to the polypeptides and polypeptide fragments of theinvention, e.g., having an amino acid sequence encoded by any one of SEQID NOs: 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or a portionthereof, or having an amino acid sequence encoded by a nucleic acidmolecule comprising all or a portion of SEQ ID NO: 1 which mayoptionally comprise at least one polymorphism shown in Table 1 (e.g.,any one of SEQ ID NOs: 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, oranother isoform or splicing variant or portion thereof). The term“antibody” as used herein refers to immunoglobulin molecules andimmunologically active portions of immunoglobulin molecules, i.e.,molecules that contain an antigen binding site that specifically bindsan antigen. A molecule that specifically binds to a polypeptide of theinvention is a molecule that binds to that polypeptide or a fragmentthereof, but does not substantially bind other molecules in a sample,e.g., a biological sample, which naturally contains the polypeptide.Examples of immunologically active portions of immunoglobulin moleculesinclude F(ab) and F(ab′)₂ fragments which can be generated by treatingthe antibody with an enzyme such as pepsin. The invention providespolyclonal and monoclonal antibodies that bind to a polypeptide of theinvention. The term “monoclonal antibody” or “monoclonal antibodycomposition”, as used herein, refers to a population of antibodymolecules that contain only one species of an antigen binding sitecapable of immunoreacting with a particular epitope of a polypeptide ofthe invention. A monoclonal antibody composition thus typically displaysa single binding affinity for a particular polypeptide of the inventionwith which it immunoreacts.

[0075] Polyclonal antibodies can be prepared as described above byimmunizing a suitable subject with a desired immunogen, e.g.,polypeptide of the invention or fragment thereof. The antibody titer inthe immunized subject can be monitored over time by standard techniques,such as with an enzyme linked immunosorbent assay (ELISA) usingimmobilized polypeptide. If desired, the antibody molecules directedagainst the polypeptide can be isolated from the mammal (e.g., from theblood) and further purified by well-known techniques, such as protein Achromatography to obtain the IgG fraction. At an appropriate time afterimmunization, e.g., when the antibody titers are highest,antibody-producing cells can be obtained from the subject and used toprepare monoclonal antibodies by standard techniques, such as thehybridoma technique originally described by Kohler and Milstein (1975)Nature, 256:495-497, the human B cell hybridoma technique (Kozbor et al.(1983) Immunol. Today, 4:72), the EBV-hybridoma technique (Cole et al.(1985), Monoclonal Antibodies and Cancer Therapy, Alan R. Liss, Inc.,pp. 77-96) or trioma techniques. The technology for producing hybridomasis well known (see generally Current Protocols in Immunology (1994)Coligan et al. (eds.) John Wiley & Sons, Inc., New York, N.Y.). Briefly,an immortal cell line (typically a myeloma) is fused to lymphocytes(typically splenocytes) from a mammal immunized with an immunogen asdescribed above, and the culture supernatants of the resulting hybridomacells are screened to identify a hybridoma producing a monoclonalantibody that binds a polypeptide of the invention.

[0076] Any of the many well known protocols used for fusing lymphocytesand immortalized cell lines can be applied for the purpose of generatinga monoclonal antibody to a polypeptide of the invention (see, e.g.,Current Protocols in Immunology, supra; Galfre et al. (1977) Nature,266:55052; R. H. Kenneth, in Monoclonal Antibodies: A New Dimension InBiological Analyses, Plenum Publishing Corp., New York, N.Y. (1980); andLerner (1981) Yale J. Biol. Med., 54:387-402. Moreover, the ordinarilyskilled worker will appreciate that there are many variations of suchmethods that also would be useful.

[0077] Alternative to preparing monoclonal antibody-secretinghybridomas, a monoclonal antibody to a polypeptide of the invention canbe identified and isolated by screening a recombinant combinatorialimmunoglobulin library (e.g., an antibody phage display library) withthe polypeptide to thereby isolate immunoglobulin library members thatbind the polypeptide. Kits for generating and screening phage displaylibraries are commercially available (e.g., the Pharmacia RecombinantPhage Antibody System, Catalog No. 27-9400-01; and the StratageneSurfZAP™ Phage Display Kit, Catalog No. 240612). Additionally, examplesof methods and reagents particularly amenable for use in generating andscreening antibody display library can be found in, for example, U.S.Pat. No. 5,223,409; PCT Publication No. WO 92/18619; PCT Publication No.WO 91/17271; PCT Publication No. WO 92/20791; PCT Publication No. WO92/15679; PCT Publication No. WO 93/01288; PCT Publication No. WO92/01047; PCT Publication No. WO 92/09690; PCT Publication No. WO90/02809; Fuchs et al. (1991) Bio/Technology, 9:1370-1372; Hay et al.(1992) Hum. Antibod. Hybridomas, 3:81-85; Huse et al. (1989) Science,246:1275-1281; Griffiths et al. (1993) EMBO J., 12:725-734.

[0078] Additionally, recombinant antibodies, such as chimeric andhumanized monoclonal antibodies, comprising both human and non-humanportions, which can be made using standard recombinant DNA techniques,are within the scope of the invention. Such chimeric and humanizedmonoclonal antibodies can be produced by recombinant DNA techniquesknown in the art.

[0079] The invention also is intended to cover human antibodies. Theirmethods for production, isolation purification and use are known tothose skilled in the art using standard methodologies.

[0080] In general, antibodies of the invention (e.g., a monoclonalantibody) can be used to isolate a polypeptide of the invention bystandard techniques, such as affinity chromatography orimmunoprecipitation. A polypeptide-specific antibody can facilitate thepurification of natural polypeptide from cells and of recombinantlyproduced polypeptide expressed in host cells. Moreover, an antibodyspecific for a polypeptide of the invention can be used to detect thepolypeptide (e.g., in a cellular lysate, cell supernatant, or tissuesample) in order to evaluate the abundance and pattern of expression ofthe polypeptide. Antibodies can be used diagnostically to monitorprotein levels in tissue as part of a clinical testing procedure, e.g.,to, for example, determine the efficacy of a given treatment regimen.Detection can be facilitated by coupling the antibody to a detectablesubstance. Examples of detectable substances include various enzymes,prosthetic groups, fluorescent materials, luminescent materials,bioluminescent materials, and radioactive materials. Examples ofsuitable enzymes include horseradish peroxidase, alkaline phosphatase,β-galactosidase, or acetylcholinesterase; examples of suitableprosthetic group complexes include streptavidin/biotin andavidin/biotin; examples of suitable fluorescent materials includeumbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine,dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin; anexample of a luminescent material includes luminol; examples ofbioluminescent materials include luciferase, luciferin, and aequorin,and examples of suitable radioactive material include 125I, 131I, 35S or3H.

[0081] Diagnostic and Screening Assays of the Invention

[0082] The present invention also pertains to a method of diagnosing oraiding in the diagnosis of peripheral arterial occlusive diseaseassociated with the presence of the PAOD1 gene or gene product in anindividual. Diagnostic assays can be designed for assessing PAOD1 geneexpression, or for assessing activity of PAOD1 polypeptides of theinvention. In one embodiment, the assays are used in the context of abiological sample (e.g., blood, serum, cells, tissue) to therebydetermine whether an individual is afflicted with peripheral arterialocclusive disease, or is at risk for (has a predisposition for or asusceptibility to) developing peripheral arterial occlusive disease. Theinvention also provides for prognostic (or predictive) assays fordetermining whether an individual is susceptible to developingperipheral arterial occlusive disease. For example, alterations in anucleic acid can be assayed in a biological sample. Such assays can beused for prognostic or predictive purpose to thereby prophylacticallytreat an individual prior to the onset of symptoms associated withperipheral arterial occlusive disease. Another aspect of the inventionpertains to assays for monitoring the influence of agents (e.g., drugs,compounds or other agents) on nucleic acid expression or activity ofpolypeptides of the invention, as well as to assays for identifyingagents which bind to PAOD1 polypeptides. These and other assays andagents are described in further detail in the following sections.

[0083] Diagnostic Assays

[0084] The nucleic acids, probes, primers, polypeptides and antibodiesdescribed herein can be used in methods of diagnosis of a susceptibilityto peripheral arterial occlusive disease, as well as in kits useful fordiagnosis of a susceptibility to peripheral arterial occlusive disease.

[0085] In one embodiment, the kit useful for diagnosis of a peripheralarterial occlusive condition associated with a PAOD1 nucleic acid or asusceptibility to a peripheral arterial occlusive condition associatedwith a PAOD1 nucleic acid comprises primers which contain one or more ofthe SNPs identified in Table 1.

[0086] In one embodiment of the invention, diagnosis of a susceptibilityto peripheral arterial occlusive disease is made by detecting apolymorphism in PAOD1 as described herein. The polymorphism can be achange in PAOD1, such as the insertion or deletion of a singlenucleotide, or of more than one nucleotide, resulting in a frame shiftmutation; the change of at least one nucleotide, resulting in a changein the encoded amino acid; the change of at least one nucleotide,resulting in the generation of a premature stop codon; the deletion ofseveral nucleotides, resulting in a deletion of one or more amino acidsencoded by the nucleotides; the insertion of one or several nucleotides,such as by unequal recombination or gene conversion, resulting in aninterruption of the coding sequence of the gene; duplication of all or apart of the nucleic acid; transposition of all or a part of the gene; orrearrangement of all or a part of the nucleic acid. More than one suchchange may be present in a single gene. Such sequence changes cause adifference in the polypeptide encoded by a PAOD1 nucleic acid. Forexample, if the difference is a frame shift change, the frame shift canresult in a change in the encoded amino acids, and/or can result in thegeneration of a premature stop codon, causing generation of a truncatedpolypeptide. Alternatively, a polymorphism associated with asusceptibility to peripheral arterial occlusive disease can be asynonymous alteration in one or more nucleotides (i.e., an alterationthat does not result in a change in the polypeptide encoded by a PAOD1nucleic acid). Such a polymorphism may alter splicing sites, affect thestability or transport of mRNA, or otherwise affect the transcription ortranslation of the gene. A PAOD1 nucleic acid that has any of thealterations described above is referred to herein as a “altered nucleicacid.”

[0087] In a first method of diagnosing a susceptibility to peripheralarterial occlusive disease, hybridization methods, such as Southernanalysis, Northern analysis, or in situ hybridizations, can be used (seeCurrent Protocols in Molecular Biology, Ausubel, F. et al., eds., JohnWiley & Sons, including all supplements through 1999). For example, abiological sample from a test subject (a “test sample”) of genomic DNA,RNA, or cDNA, is obtained from an individual suspected of having, beingsusceptible to or predisposed for, or carrying a defect for, peripheralarterial occlusive disease (the “test individual”). The individual canbe an adult, child, or fetus. The test sample can be from any sourcewhich contains genomic DNA, such as a blood sample, sample of amnioticfluid, sample of cerebrospinal fluid, or tissue sample from skin,muscle, buccal or conjunctival mucosa, placenta, gastrointestinal tractor other organs. A test sample of DNA from fetal cells or tissue can beobtained by appropriate methods, such as by amniocentesis or chorionicvillus sampling. The DNA, RNA, or cDNA sample is then examined todetermine whether a polymorphism in PAOD1 is present, and/or todetermine which splicing variant(s) encoded by PAOD1 is present. Thepresence of the polymorphism or splicing variant(s) can be indicated byhybridization of the gene in the genomic DNA, RNA, or cDNA to a nucleicacid probe. A “nucleic acid probe”, as used herein, can be a DNA probeor an RNA probe; the nucleic acid probe can contain at least onepolymorphism in PAOD1 or contains a nucleic acid encoding a particularsplicing variant of PAOD1. The probe can be any of the nucleic acidmolecules described above (e.g., the gene or nucleic acid, a fragment, avector comprising the gene, a probe or primer, etc.).

[0088] To diagnose a susceptibility to peripheral arterial occlusivedisease, a hybridization sample is formed by contacting the test samplecontaining PAOD1, with at least one nucleic acid probe. A preferredprobe for detecting mRNA or genomic DNA is a labeled nucleic acid probecapable of hybridizing to mRNA or genomic DNA sequences describedherein. The nucleic acid probe can be, for example, a full-lengthnucleic acid molecule, or a portion thereof, such as an oligonucleotideof at least 15, 30, 50, 100, 250 or 500 nucleotides in length andsufficient to specifically hybridize under stringent conditions toappropriate mRNA or genomic DNA. For example, the nucleic acid probe canbe all or a portion of SEQ ID NO: 1 which may optionally comprise atleast one polymorphism shown in Table 1, or the complement thereof, or aportion thereof; or can be a nucleic acid encoding a portion of any oneof SEQ ID NOs: 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36. Othersuitable probes for use in the diagnostic assays of the invention aredescribed above (see e.g., probes and primers discussed under theheading, “Nucleic Acids of the Invention”).

[0089] The hybridization sample is maintained under conditions which aresufficient to allow specific hybridization of the nucleic acid probe toPAOD1. “Specific hybridization”, as used herein, indicates exacthybridization (e.g., with no mismatches). Specific hybridization can beperformed under high stringency conditions or moderate stringencyconditions, for example, as described above. In a particularly preferredembodiment, the hybridization conditions for specific hybridization arehigh stringency.

[0090] Specific hybridization, if present, is then detected usingstandard methods. If specific hybridization occurs between the nucleicacid probe and PAOD1 in the test sample, then PAOD1 has thepolymorphism, or is the splicing variant, that is present in the nucleicacid probe. More than one nucleic acid probe can also be usedconcurrently in this method. Specific hybridization of any one of thenucleic acid probes is indicative of a polymorphism in PAOD1, or of thepresence of a particular splicing variant encoding PAOD1 and istherefore diagnostic for a susceptibility to peripheral arterialocclusive disease.

[0091] In Northern analysis (see Current Protocols in Molecular Biology,Ausubel, F. et al., eds., John Wiley & Sons, supra) the hybridizationmethods described above are used to identify the presence of apolymorphism or a particular splicing variant, associated with asusceptibility to peripheral arterial occlusive disease. For Northernanalysis, a test sample of RNA is obtained from the individual byappropriate means. Specific hybridization of a nucleic acid probe, asdescribed above, to RNA from the individual is indicative of apolymorphism in PAOD1, or of the presence of a particular splicingvariant encoded by PAOD1, and is therefore diagnostic for asusceptibility to peripheral arterial occlusive disease.

[0092] For representative examples of use of nucleic acid probes, see,for example, U.S. Pat. Nos. 5,288,611 and 4,851,330.

[0093] Alternatively, a peptide nucleic acid (PNA) probe can be usedinstead of a nucleic acid probe in the hybridization methods describedabove. PNA is a DNA mimic having a peptide-like, inorganic backbone,such as N-(2-aminoethyl)glycine units, with an organic base (A, G, C, Tor U) attached to the glycine nitrogen via a methylene carbonyl linker(see, for example, Nielsen, P. E. et al., Bioconjugate Chemistry, 1994,5, American Chemical Society, p.1 (1994). The PNA probe can be designedto specifically hybridize to a gene having a polymorphism associatedwith a susceptibility to peripheral arterial occlusive disease.Hybridization of the PNA probe to PAOD1 is diagnostic for asusceptibility to peripheral arterial occlusive disease.

[0094] In another method of the invention, mutation analysis byrestriction digestion can be used to detect a mutant gene, or genescontaining a polymorphism(s), if the mutation or polymorphism in thegene results in the creation or elimination of a restriction site. Atest sample containing genomic DNA is obtained from the individual.Polymerase chain reaction (PCR) can be used to amplify PAOD1 (and, ifnecessary, the flanking sequences) in the test sample of genomic DNAfrom the test individual. RFLP analysis is conducted as described (seeCurrent Protocols in Molecular Biology, supra). The digestion pattern ofthe relevant DNA fragment indicates the presence or absence of themutation or polymorphism in PAOD1, and therefore indicates the presenceor absence of this susceptibility to peripheral arterial occlusivedisease.

[0095] Sequence analysis can also be used to detect specificpolymorphisms in PAOD1. A test sample of DNA or RNA is obtained from thetest individual. PCR or other appropriate methods can be used to amplifythe gene, and/or its flanking sequences, if desired. The sequence ofPAOD1, or a fragment of the gene, or cDNA, or fragment of the cDNA, ormRNA, or fragment of the mRNA, is determined, using standard methods.The sequence of the gene, gene fragment, cDNA, cDNA fragment, mRNA, ormRNA fragment is compared with the known nucleic acid sequence of thegene, cDNA (e.g., SEQ ID NO: 1 which may optionally comprise at leastone polymorphism shown in Table 1, or a nucleic acid sequence encodingany one of SEQ ID NOs: 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or afragment thereof) or mRNA, as appropriate. The presence of apolymorphism in PAOD1 indicates that the individual has a susceptibilityto peripheral arterial occlusive disease.

[0096] Allele-specific oligonucleotides can also be used to detect thepresence of a polymorphism in PAOD1, through the use of dot-blothybridization of amplified oligonucleotides with allele-specificoligonucleotide (ASO) probes (see, for example, Saiki, R. et al.,(1986), Nature (London) 324:163-166). An “allele-specificoligonucleotide” (also referred to herein as an “allele-specificoligonucleotide probe”) is an oligonucleotide of approximately 10-50base pairs, preferably approximately 15-30 base pairs, that specificallyhybridizes to PAOD1, and that contains a polymorphism associated with asusceptibility to peripheral arterial occlusive disease. Anallele-specific oligonucleotide probe that is specific for particularpolymorphisms in PAOD1 can be prepared, using standard methods (seeCurrent Protocols in Molecular Biology, supra). To identifypolymorphisms in the gene that are associated with a susceptibility toperipheral arterial occlusive disease, a test sample of DNA is obtainedfrom the individual. PCR can be used to amplify all or a fragment ofPAOD1, and its flanking sequences. The DNA containing the amplifiedPAOD1 (or fragment of the gene) is dot-blotted, using standard methods(see Current Protocols in Molecular Biology, supra), and the blot iscontacted with the oligonucleotide probe. The presence of specifichybridization of the probe to the amplified PAOD1 is then detected.Specific hybridization of an allele-specific oligonucleotide probe toDNA from the individual is indicative of a polymorphism in PAOD1, and istherefore indicative of a susceptibility to peripheral arterialocclusive disease.

[0097] The invention further provides allele-specific oligonucleotidesthat hybridize to the reference or variant allele of a gene or nucleicacid comprising a single nucleotide polymorphism or to the complementthereof. These oligonucleotides can be probes or primers.

[0098] An allele-specific primer hybridizes to a site on target DNAoverlapping a polymorphism and only primes amplification of an allelicform to which the primer exhibits perfect complementarity. See Gibbs,Nucleic Acid Res. 17, 2427-2448 (1989). This primer is used inconjunction with a second primer, which hybridizes at a distal site.Amplification proceeds from the two primers, resulting in a detectableproduct, which indicates the particular allelic form is present. Acontrol is usually performed with a second pair of primers, one of whichshows a single base mismatch at the polymorphic site and the other ofwhich exhibits perfect complementarity to a distal site. The single-basemismatch prevents amplification and no detectable product is formed. Themethod works best when the mismatch is included in the 3′-most positionof the oligonucleotide aligned with the polymorphism because thisposition is most destabilizing to elongation from the primer (see, e.g.,WO 93/22456).

[0099] With the addition of such analogs as locked nucleic acids (LNAs),the size of primers and probes can be reduced to as few as 8 bases. LNAsare a novel class of bicyclic DNA analogs in which the 2′ and 4′positions in the furanose ring are joined via an O-methylene (oxy-LNA),S-methylene (thio-LNA), or amino methylene (amino-LNA) moiety. Common toall of these LNA variants is an affinity toward complementary nucleicacids, which is by far the highest reported for a DNA analog. Forexample, particular all oxy-LNA nonamers have been shown to have meltingtemperatures of 64° C. and 74° C. when in complex with complementary DNAor RNA, respectively, as oposed to 28° C. for both DNA and RNA for thecorresponding DNA nonamer. Substantial increases in T_(m) are alsoobtained when LNA monomers are used in combination with standard DNA orRNA monomers. For primers and probes, depending on where the LNAmonomers are included (e.g., the 3′ end, the 5′end, or in the middle),the T_(m) could be increased considerably.

[0100] In another embodiment, arrays of oligonucleotide probes that arecomplementary to target nucleic acid sequence segments from anindividual, can be used to identify polymorphisms in PAOD1. For example,in one embodiment, an oligonucleotide array can be used. Oligonucleotidearrays typically comprise a plurality of different oligonucleotideprobes that are coupled to a surface of a substrate in different knownlocations. These oligonucleotide arrays, also described as“Genechips.TM.,” have been generally described in the art, for example,U.S. Pat. No. 5,143,854 and PCT patent publication Nos. WO 90/15070 and92/10092. These arrays can generally be produced using mechanicalsynthesis methods or light directed synthesis methods which incorporatea combination of photolithographic methods and solid phaseoligonucleotide synthesis methods. See Fodor et al., Science,251:767-777 (1991), Pirrung et al., U.S. Pat. No. 5,143,854 (see alsoPCT Application No. WO 90/15070) and Fodor et al., PCT Publication No.WO 92/10092 and U.S. Pat. No. 5,424,186, the entire teachings of each ofwhich are incorporated by reference herein. Techniques for the synthesisof these arrays using mechanical synthesis methods are described in,e.g., U.S. Pat. No. 5,384,261, the entire teachings of which areincorporated by reference herein. In another example, linear arrays canbe utilized.

[0101] Once an oligonucleotide array is prepared, a nucleic acid ofinterest is hybridized with the array and scanned for polymorphisms.Hybridization and scanning are generally carried out by methodsdescribed herein and also in, e.g., Published PCT Application Nos. WO92/10092 and WO 95/11995, and U.S. Pat. No. 5,424,186, the entireteachings of which are incorporated by reference herein. In brief, atarget nucleic acid sequence which includes one or more previouslyidentified polymorphic markers is amplified by well known amplificationtechniques, e.g., PCR. Typically, this involves the use of primersequences that are complementary to the two strands of the targetsequence both upstream and downstream from the polymorphism. AsymmetricPCR techniques may also be used. Amplified target, generallyincorporating a label, is then hybridized with the array underappropriate conditions. Upon completion of hybridization and washing ofthe array, the array is scanned to determine the position on the arrayto which the target sequence hybridizes. The hybridization data obtainedfrom the scan is typically in the form of fluorescence intensities as afunction of location on the array.

[0102] Although primarily described in terms of a single detectionblock, e.g., for detection of a single polymorphism, arrays can includemultiple detection blocks, and thus be capable of analyzing multiple,specific polymorphisms. In alternate arrangements, it will generally beunderstood that detection blocks may be grouped within a single array orin multiple, separate arrays so that varying, optimal conditions may beused during the hybridization of the target to the array. For example,it may often be desirable to provide for the detection of thosepolymorphisms that fall within G-C rich stretches of a genomic sequence,separately from those falling in A-T rich segments. This allows for theseparate optimization of hybridization conditions for each situation.

[0103] Additional description of use of oligonucleotide arrays fordetection of polymorphisms can be found, for example, in U.S. Pat. Nos.5,858,659 and 5,837,832, the entire teachings of which are incorporatedby reference herein.

[0104] Other methods of nucleic acid analysis can be used to detectpolymorphisms in PAOD1 or splicing variants encoded by PAOD1.Representative methods include direct manual sequencing (Church andGilbert, (1988), Proc. Natl. Acad. Sci. USA 81:1991-1995; Sanger, F. etal. (1977) Proc. Natl. Acad. Sci. 74:5463-5467; Beavis et al. U.S. Pat.No. 5,288,644); automated fluorescent sequencing; single-strandedconformation polymorphism assays (SSCP); clamped denaturing gelelectrophoresis (CDGE); denaturing gradient gel electrophoresis (DGGE)(Sheffield, V. C. et al. (19891) Proc. Natl. Acad. Sci. USA 86:232-236),mobility shift analysis (Orita, M. et al. (1989) Proc. Natl. Acad. Sci.USA 86:2766-2770), restriction enzyme analysis (Flavell et al. (1978)Cell 15:25; Geever, et al. (1981) Proc. Natl Acad. Sci. USA 78:5081);heteroduplex analysis; chemical mismatch cleavage (CMC) (Cotton et al.(1985) Proc. Natl. Acad. Sci. USA 85:4397-4401); RNase protection assays(Myers, R. M. et al. (1985) Science 230:1242); use of polypeptides whichrecognize nucleotide mismatches, such as E. coli mutS protein;allele-specific PCR, for example.

[0105] In one embodiment of the invention, diagnosis of a disease orcondition associated with a PAOD1 nucleic acid (e.g., peripheralarterial occlusive disease) or a susceptibility to a disease orcondition associated with a PAOD1 nucleic acid (e.g., peripheralarterial occlusive disease) can also be made by expression analysis byquantitative PCR (kinetic thermal cycling). This technique, utilizingTaqMan can be used to allow the identification of polymorphisms andwhether a patient is homozygous or heterozygous. The technique canassess the presence of an alteration in the expression or composition ofthe polypeptide encoded by a PAOD1 nucleic acid or splicing variantsencoded by a PAOD1 nucleic acid. Further, the expression of the variantscan be quantified as physically or functionally different.

[0106] In another embodiment of the invention, diagnosis of asusceptibility to peripheral arterial occlusive disease can also be madeby examining expression and/or composition of an PAOD1 polypeptide, by avariety of methods, including enzyme linked immunosorbent assays(ELISAs), Western blots, immunoprecipitations and immunofluorescence. Atest sample from an individual is assessed for the presence of analteration in the expression and/or an alteration in composition of thepolypeptide encoded by PAOD1, or for the presence of a particularvariant encoded by PAOD1. An alteration in expression of a polypeptideencoded by PAOD1 can be, for example, an alteration in the quantitativepolypeptide expression (i.e., the amount of polypeptide produced); analteration in the composition of a polypeptide encoded by PAOD1 is analteration in the qualitative polypeptide expression (e.g., expressionof a mutant PAOD1 polypeptide or of a different splicing variant). In apreferred embodiment, diagnosis of a susceptibility to peripheralarterial occlusive disease is made by detecting a particular splicingvariant encoded by PAOD1, or a particular pattern of splicing variants.

[0107] Both such alterations (quantitative and qualitative) can also bepresent. An “alteration” in the polypeptide expression or composition,as used herein, refers to an alteration in expression or composition ina test sample, as compared with the expression or composition ofpolypeptide by PAOD1 in a control sample. A control sample is a samplethat corresponds to the test sample (e.g., is from the same type ofcells), and is from an individual who is not affected by peripheralarterial occlusive disease. An alteration in the expression orcomposition of the polypeptide in the test sample, as compared with thecontrol sample, is indicative of a susceptibility to peripheral arterialocclusive disease. Similarly, the presence of one or more differentsplicing variants in the test sample, or the presence of significantlydifferent amounts of different splicing variants in the test sample, ascompared with the control sample, is indicative of a susceptibility toperipheral arterial occlusive disease. Various means of examiningexpression or composition of the polypeptide encoded by PAOD1 can beused, including spectroscopy, colorimetry, electrophoresis, isoelectricfocusing, and immunoassays (e.g., David et al., U.S. Pat. No. 4,376,110)such as immunoblotting (see also Current Protocols in Molecular Biology,particularly chapter 10). For example, in one embodiment, an antibodycapable of binding to the polypeptide (e.g., as described above),preferably an antibody with a detectable label, can be used. Antibodiescan be polyclonal, or more preferably, monoclonal. An intact antibody,or a fragment thereof (e.g., Fab or F(ab′)₂) can be used. The term“labeled”, with regard to the probe or antibody, is intended toencompass direct labeling of the probe or antibody by coupling (i.e.,physically linking) a detectable substance to the probe or antibody, aswell as indirect labeling of the probe or antibody by reactivity withanother reagent that is directly labeled. Examples of indirect labelinginclude detection of a primary antibody using a fluorescently labeledsecondary antibody and end-labeling of a DNA probe with biotin such thatit can be detected with fluorescently labeled streptavidin.

[0108] Western blotting analysis, using an antibody as described abovethat specifically binds to a polypeptide encoded by an altered PAOD1, oran antibody that specifically binds to a polypeptide encoded by anon-altered nucleic acid, or an antibody that specifically binds to aparticular splicing variant encoded by PAOD1, can be used to identifythe presence in a test sample of a particular splicing variant or of apolypeptide encoded by a polymorphic or altered PAOD1, or the absence ina test sample of a particular splicing variant or of a polypeptideencoded by a non-polymorphic or non-altered acid. The presence of apolypeptide encoded by a polymorphic or altered nucleic acid, or theabsence of a polypeptide encoded by a non-polymorphic or non-alterednucleic acid, is diagnostic for a susceptibility to peripheral arterialocclusive disease, as is the presence (or absence) of particularsplicing variants encoded by the PAOD1 nucleic acid.

[0109] In one embodiment of this method, the level or amount ofpolypeptide encoded by PAOD1 in a test sample is compared with the levelor amount of the polypeptide encoded by PAOD1 in a control sample. Alevel or amount of the polypeptide in the test sample that is higher orlower than the level or amount of the polypeptide in the control sample,such that the difference is statistically significant, is indicative ofan alteration in the expression of the polypeptide encoded by PAOD1, andis diagnostic for a susceptibility to peripheral arterial occlusivedisease. Alternatively, the composition of the polypeptide encoded byPAOD1 in a test sample is compared with the composition of thepolypeptide encoded by PAOD1 in a control sample (e.g., the presence ofdifferent splicing variants). A difference in the composition of thepolypeptide in the test sample, as compared with the composition of thepolypeptide in the control sample, is diagnostic for a susceptibility toperipheral arterial occlusive disease. In another embodiment, both thelevel or amount and the composition of the polypeptide can be assessedin the test sample and in the control sample. A difference in the amountor level of the polypeptide in the test sample, compared to the controlsample; a difference in composition in the test sample, compared to thecontrol sample; or both a difference in the amount or level, and adifference in the composition, is indicative of a susceptibility toperipheral arterial occlusive disease.

[0110] The invention further pertains to a method for the diagnosis oridentification of a susceptibility to peripheral arterial occlusivedisease in an individual, by identifying an at-risk haplotype (e.g., ahaplotype comprising a PAOD1 nucleic acid). In one embodiment, theat-risk haplotype is one that confers a significant risk of peripheralarterial occlusive disease. In one embodiment, significance associatedwith a haplotype is measured by an odds ratio. In a further embodiment,the significance is measured by a percentage. In one embodiment, asignificant risk is measured as an odds ratio of at least about 2.2,including by not limited to: 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, and 1.9.In a further embodiment, an odds ratio of at least 1.2 is significant.In a further embodiment, an odds ratio of at least about 1.5 issignificant. In a further embodiment, a significant increase in risk isat least about 1.7 is significant. In a further embodiment, asignificant increase in risk is at least about 20%, including but notlimited to about 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%,80%, 85%, 90%, 95%, and 98%. In a further embodiment, a significantincrease in risk is at least about 50%. It is understood however, thatidentifying whether a risk is medically significant may also depend on avariety of factors, including the specific disease, the haplotype, andoften, environmental factors.

[0111] The invention also pertains to methods of diagnosing peripheralarterial occlusive disease or a susceptibility to peripheral arterialocclusive disease in an individual, comprising screening for an at-riskhaplotype in the PAOD1 nucleic acid that is more frequently present inan individual susceptible to peripheral arterial occlusive disease(affected), compared to the frequency of its presence in a healthyindividual (control), wherein the presence of the haplotype isindicative of peripheral arterial occlusive disease or susceptibility toperipheral arterial occlusive disease. Standard techniques forgenotyping for the presence of SNPs and/or microsatellite markers thatare associated with peripheral arterial occlusive disease can be used,such as fluorescent based techniques (Chen, et al., Genome Res. 9, 492(1999), PCR, LCR, Nested PCR and other techniques for nucleic acidamplification. In a preferred embodiment, the method comprises assessingin an individual the presence or frequency of SNPs and/ormicrosatellites in the PAOD1 nucleic acid that are associated withperipheral arterial occlusive disease, wherein an excess or higherfrequency of the SNPs and/or microsatellites compared to a healthycontrol individual is indicative that the individual has peripheralarterial occlusive disease or is susceptible to peripheral arterialocclusive disease.

[0112] Kits (e.g., reagent kits) useful in the methods of diagnosiscomprise components useful in any of the methods described herein,including for example, hybridization probes or primers as describedherein (e.g., labeled probes or primers), reagents for detection oflabeled molecules, restriction enzymes (e.g., for RFLP analysis),allele-specific oligonucleotides, antibodies which bind to altered or tonon-altered (native) PAOD1 polypeptide, means for amplification ofnucleic acids comprising PAOD1, or means for analyzing the nucleic acidsequence of PAOD1 or for analyzing the amino acid sequence of an PAOD1polypeptide, etc.

[0113] Screening Assays and Agents Identified Thereby

[0114] The invention provides methods (also referred to herein as“screening assays”) for identifying the presence of a nucleotide thathybridizes to a nucleic acid of the invention, as well as foridentifying the presence of a polypeptide encoded by a nucleic acid ofthe invention. In one embodiment, the presence (or absence) of a nucleicacid molecule of interest (e.g., a nucleic acid that has significanthomology with a nucleic acid of the invention) in a sample can beassessed by contacting the sample with a nucleic acid comprising anucleic acid of the invention (e.g., a nucleic acid having the sequenceof SEQ ID NO: 1 which may optionally comprise at least one polymorphismshown in Table 1, or the complement thereof, or a nucleic acid encodingan amino acid having the sequence of any one of SEQ ID NOs: 16, 18, 20,22, 24, 26, 28, 30, 32, 34, 36, or a fragment or variant of such nucleicacids), under stringent conditions as described above, and thenassessing the sample for the presence (or absence) of hybridization. Ina preferred embodiment, high stringency conditions are conditionsappropriate for selective hybridization. In another embodiment, a samplecontaining the nucleic acid molecule of interest is contacted with anucleic acid containing a contiguous nucleotide sequence (e.g., a primeror a probe as described above) that is at least partially complementaryto a part of the nucleic acid molecule of interest (e.g., a PAOD1nucleic acid), and the contacted sample is assessed for the presence orabsence of hybridization. In a preferred embodiment, the nucleic acidcontaining a contiguous nucleotide sequence is completely complementaryto a part of the nucleic acid molecule of interest.

[0115] In any of these embodiment, all or a portion of the nucleic acidof interest can be subjected to amplification prior to performing thehybridization.

[0116] In another embodiment, the presence (or absence) of a polypeptideof interest, such as a polypeptide of the invention or a fragment orvariant thereof, in a sample can be assessed by contacting the samplewith an antibody that specifically hybridizes to the polypeptide ofinterest (e.g., an antibody such as those described above), and thenassessing the sample for the presence (or absence) of binding of theantibody to the polypeptide of interest.

[0117] In another embodiment, the invention provides methods foridentifying agents (e.g., fusion proteins, polypeptides,peptidomimetics, prodrugs, receptors, binding agents, antibodies, smallmolecules or other drugs, or ribozymes which alter (e.g., increase ordecrease) the activity of the polypeptides described herein, or whichotherwise interact with the polypeptides herein. For example, suchagents can be agents which bind to polypeptides described herein (e.g.,PAOD1 binding agents); which have a stimulatory or inhibitory effect on,for example, activity of polypeptides of the invention; or which change(e.g., enhance or inhibit) the ability of the polypeptides of theinvention to interact with PAOD1 binding agents (e.g., receptors orother binding agents); or which alter posttranslational processing ofthe PAOD1 polypeptide (e.g., agents that alter proteolytic processing todirect the polypeptide from where it is normally synthesized to anotherlocation in the cell, such as the cell surface; agents that alterproteolytic processing such that more polypeptide is released from thecell, etc.

[0118] In one embodiment, the invention provides assays for screeningcandidate or test agents that bind to or modulate the activity ofpolypeptides described herein (or biologically active portion(s)thereof), as well as agents identifiable by the assays. Test agents canbe obtained using any of the numerous approaches in combinatoriallibrary methods known in the art, including: biological libraries;spatially addressable parallel solid phase or solution phase libraries;synthetic library methods requiring deconvolution; the ‘one-beadone-compound’ library method; and synthetic library methods usingaffinity chromatography selection. The biological library approach islimited to polypeptide libraries, while the other four approaches areapplicable to polypeptide, non-peptide oligomer or small moleculelibraries of compounds (Lam, K. S. (1997) Anticancer Drug Des., 12:145).

[0119] In one embodiment, to identify agents which alter the activity ofa PAOD1 polypeptide, a cell, cell lysate, or solution containing orexpressing a PAOD1 polypeptide (e.g., any one of SEQ ID NOs: 16, 18, 20,22, 24, 26, 28, 30, 32, 34, 36, or another splicing variant encoded byPAOD1), or a fragment or derivative thereof (as described above), can becontacted with an agent to be tested; alternatively, the polypeptide canbe contacted directly with the agent to be tested. The level (amount) ofPAOD1 activity is assessed (e.g., the level (amount) of PAOD1 activityis measured, either directly or indirectly), and is compared with thelevel of activity in a control (i.e., the level of activity of the PAOD1polypeptide or active fragment or derivative thereof in the absence ofthe agent to be tested). If the level of the activity in the presence ofthe agent differs, by an amount that is statistically significant, fromthe level of the activity in the absence of the agent, then the agent isan agent that alters the activity of PAOD1 polypeptide. An increase inthe level of PAOD1 activity relative to a control, indicates that theagent is an agent that enhances (is an agonist of) PAOD1 activity.Similarly, a decrease in the level of PAOD1 activity relative to acontrol, indicates that the agent is an agent that inhibits (is anantagonist of) PAOD1 activity. In another embodiment, the level ofactivity of a PAOD1 polypeptide or derivative or fragment thereof in thepresence of the agent to be tested, is compared with a control levelthat has previously been established. A level of the activity in thepresence of the agent that differs from the control level by an amountthat is statistically significant indicates that the agent alters PAOD1activity.

[0120] The present invention also relates to an assay for identifyingagents which alter the expression of the PAOD1 gene (e.g., antisensenucleic acids, fusion proteins, polypeptides, peptidomimetics, prodrugs,receptors, binding agents, antibodies, small molecules or other drugs,or ribozymes) which alter (e.g., increase or decrease) expression (e.g.,transcription or translation) of the gene or which otherwise interactwith the nucleic acids described herein, as well as agents identifiableby the assays. For example, a solution containing a nucleic acidencoding PAOD1 polypeptide (e.g., PAOD1 gene) can be contacted with anagent to be tested. The solution can comprise, for example, cellscontaining the nucleic acid or cell lysate containing the nucleic acid;alternatively, the solution can be another solution which compriseselements necessary for transcription/translation of the nucleic acid.Cells not suspended in solution can also be employed, if desired. Thelevel and/or pattern of PAOD1 expression (e.g., the level and/or patternof mRNA or of protein expressed, such as the level and/or pattern ofdifferent splicing variants) is assessed, and is compared with the leveland/or pattern of expression in a control (i.e., the level and/orpattern of the PAOD1 expression in the absence of the agent to betested). If the level and/or pattern in the presence of the agentdiffers, by an amount or in a manner that is statistically significant,from the level and/or pattern in the absence of the agent, then theagent is an agent that alters the expression of PAOD1. Enhancement ofPAOD1 expression indicates that the agent is an agonist of PAOD1activity. Similarly, inhibition of PAOD1 expression indicates that theagent is an antagonist of PAOD1 activity. In another embodiment, thelevel and/or pattern of PAOD1 polypeptide(s)(e.g., different splicingvariants) in the presence of the agent to be tested, is compared with acontrol level and/or pattern that has previously been established. Alevel and/or pattern in the presence of the agent that differs from thecontrol level and/or pattern by an amount or in a manner that isstatistically significant indicates that the agent alters PAOD1expression.

[0121] In another embodiment of the invention, agents which alter theexpression of the PAOD1 gene or which otherwise interact with thenucleic acids described herein, can be identified using a cell, celllysate, or solution containing a nucleic acid encoding the promoterregion of the PAOD1 gene operably linked to a reporter gene. Aftercontact with an agent to be tested, the level of expression of thereporter gene (e.g., the level of mRNA or of protein expressed) isassessed, and is compared with the level of expression in a control(i.e., the level of the expression of the reporter gene in the absenceof the agent to be tested). If the level in the presence of the agentdiffers, by an amount or in a manner that is statistically significant,from the level in the absence of the agent, then the agent is an agentthat alters the expression of PAOD1, as indicated by its ability toalter expression of a gene that is operably linked to the PAOD1 genepromoter. Enhancement of the expression of the reporter indicates thatthe agent is an agonist of PAOD1 activity. Similarly, inhibition of theexpression of the reporter indicates that the agent is an antagonist ofPAOD1 activity. In another embodiment, the level of expression of thereporter in the presence of the agent to be tested, is compared with acontrol level that has previously been established. A level in thepresence of the agent that differs from the control level by an amountor in a manner that is statistically significant indicates that theagent alters PAOD1 expression.

[0122] Agents which alter the amounts of different splicing variantsencoded by PAOD1 (e.g., an agent which enhances activity of a firstsplicing variant, and which inhibits activity of a second splicingvariant), as well as agents which are agonists of activity of a firstsplicing variant and antagonists of activity of a second splicingvariant, can easily be identified using these methods described above.

[0123] In other embodiments of the invention, assays can be used toassess the impact of a test agent on the activity of a polypeptide inrelation to a PAOD1 binding agent. For example, a cell that expresses acompound that interacts with PAOD1 (herein referred to as a “PAOD1binding agent”, which can be a polypeptide or other molecule thatinteracts with PAOD1, such as a receptor) is contacted with PAOD1 in thepresence of a test agent, and the ability of the test agent to alter theinteraction between PAOD1 and the PAOD1 binding agent is determined.Alternatively, a cell lysate or a solution containing the PAOD1 bindingagent, can be used. An agent which binds to PAOD1 or the PAOD1 bindingagent can alter the interaction by interfering with, or enhancing theability of PAOD1 to bind to, associate with, or otherwise interact withthe PAOD1 binding agent. Determining the ability of the test agent tobind to PAOD1 or an PAOD1 binding agent can be accomplished, forexample, by coupling the test agent with a radioisotope or enzymaticlabel such that binding of the test agent to the polypeptide can bedetermined by detecting the labeled with ¹²⁵I, ³⁵S, ¹⁴C or ³H, eitherdirectly or indirectly, and the radioisotope detected by direct countingof radioemmission or by scintillation counting. Alternatively, testagents can be enzymatically labeled with, for example, horseradishperoxidase, alkaline phosphatase, or luciferase, and the enzymatic labeldetected by determination of conversion of an appropriate substrate toproduct. It is also within the scope of this invention to determine theability of a test agent to interact with the polypeptide without thelabeling of any of the interactants. For example, a microphysiometer canbe used to detect the interaction of a test agent with PAOD1 or a PAOD1binding agent without the labeling of either the test agent, PAOD1, orthe PAOD1 binding agent. McConnell, H. M. et al. (1992) Science,257:1906-1912. As used herein, a “microphysiometer” (e.g., Cytosensor™)is an analytical instrument that measures the rate at which a cellacidifies its environment using a light-addressable potentiometricsensor (LAPS). Changes in this acidification rate can be used as anindicator of the interaction between ligand and polypeptide. Known PAOD1binding partners include Gi/Go, Gs and Gp (Namba et al., Nature,365:166-170, 1993). Thus, these receptors can be used to screen forcompounds that are PAOD1 receptor agonists for use in treatingperipheral arterial occlusive disease or PAOD1 receptor antagonists forstudying peripheral arterial occlusive disease. The linkage dataprovided herein, for the first time, provides such connection toperipheral arterial occlusive disease. Drugs can be designed to regulatePAOD1 receptor activation which in turn can be used to regulatesignaling pathways and transcription events of genes downstream, such asadynelate cyclase, MAP kinase, Rho (GTPase) Phospholipase C, NFkB.

[0124] In another embodiment of the invention, assays can be used toidentify polypeptides that interact with one or more PAOD1 polypeptides,as described herein. For example, a yeast two-hybrid system such as thatdescribed by Fields and Song (Fields, S. and Song, O., Nature340:245-246 (1989)) can be used to identify polypeptides that interactwith one or more PAOD1 polypeptides. In such a yeast two-hybrid system,vectors are constructed based on the flexibility of a transcriptionfactor which has two functional domains (a DNA binding domain and atranscription activation domain). If the two domains are separated butfused to two different proteins that interact with one another,transcriptional activation can be achieved, and transcription ofspecific markers (e.g., nutritional markers such as His and Ade, orcolor markers such as lacZ) can be used to identify the presence ofinteraction and transcriptional activation. For example, in the methodsof the invention, a first vector is used which includes a nucleic acidencoding a DNA binding domain and also an PAOD1 polypeptide, splicingvariant, or fragment or derivative thereof, and a second vector is usedwhich includes a nucleic acid encoding a transcription activation domainand also a nucleic acid encoding a polypeptide which potentially mayinteract with the PAOD1 polypeptide, splicing variant, or fragment orderivative thereof (e.g., a PAOD1 polypeptide binding agent orreceptor). Incubation of yeast containing the first vector and thesecond vector under appropriate conditions (e.g., mating conditions suchas used in the Matchmaker™ system from Clontech) allows identificationof colonies which express the markers of interest. These colonies can beexamined to identify the polypeptide(s) which interact with the PAOD1polypeptide or fragment or derivative thereof. Such polypeptides may beuseful as agents which alter the activity of expression of an PAOD1polypeptide, as described above.

[0125] In more than one embodiment of the above assay methods of thepresent invention, it may be desirable to immobilize either PAOD1, thePAOD1 binding agent, or other components of the assay on a solidsupport, in order to facilitate separation of complexed from uncomplexedforms of one or both of the polypeptides, as well as to accommodateautomation of the assay. Binding of a test agent to the polypeptide, orinteraction of the polypeptide with a binding agent in the presence andabsence of a test agent, can be accomplished in any vessel suitable forcontaining the reactants. Examples of such vessels include microtitreplates, test tubes, and micro-centrifuge tubes. In one embodiment, afusion protein (e.g., a glutathione-S-transferase fusion protein) can beprovided which adds a domain that allows PAOD1 or a PAOD1 binding agentto be bound to a matrix or other solid support.

[0126] In another embodiment, modulators of expression of nucleic acidmolecules of the invention are identified in a method wherein a cell,cell lysate, or solution containing a nucleic acid encoding PAOD1 iscontacted with a test agent and the expression of appropriate mRNA orpolypeptide (e.g., splicing variant(s)) in the cell, cell lysate, orsolution, is determined. The level of expression of appropriate mRNA orpolypeptide(s) in the presence of the test agent is compared to thelevel of expression of mRNA or polypeptide(s) in the absence of the testagent. The test agent can then be identified as a modulator ofexpression based on this comparison. For example, when expression ofmRNA or polypeptide is greater (statistically significantly greater) inthe presence of the test agent than in its absence, the test agent isidentified as a stimulator or enhancer of the mRNA or polypeptideexpression. Alternatively, when expression of the mRNA or polypeptide isless (statistically significantly less) in the presence of the testagent than in its absence, the test agent is identified as an inhibitorof the mRNA or polypeptide expression. The level of mRNA or polypeptideexpression in the cells can be determined by methods described hereinfor detecting mRNA or polypeptide.

[0127] This invention further pertains to novel agents identified by theabove-described screening assays. Accordingly, it is within the scope ofthis invention to further use an agent identified as described herein inan appropriate animal model. For example, an agent identified asdescribed herein (e.g., a test agent that is a modulating agent, anantisense nucleic acid molecule, a specific antibody, or apolypeptide-binding agent) can be used in an animal model to determinethe efficacy, toxicity, or side effects of treatment with such an agent.Alternatively, an agent identified as described herein can be used in ananimal model to determine the mechanism of action of such an agent.Furthermore, this invention pertains to uses of novel agents identifiedby the above-described screening assays for treatments as describedherein. In addition, an agent identified as described herein can be usedto alter activity of a polypeptide encoded by PAOD1, or to alterexpression of PAOD1, by contacting the polypeptide or the gene (orcontacting a cell comprising the polypeptide or the nucleic acid) withthe agent identified as described herein.

[0128] Pharmaceutical Compositions

[0129] The present invention also pertains to pharmaceuticalcompositions comprising nucleic acids described herein, particularlynucleotides encoding the polypeptides described herein; comprisingpolypeptides described herein (e.g., any one of SEQ ID NOs: 16, 18, 20,22, 24, 26, 28, 30, 32, 34, 36); and/or comprising other splicingvariants encoded by PAOD1; and/or an agent that alters (e.g., enhancesor inhibits) PAOD1 gene expression or PAOD1 polypeptide activity asdescribed herein. For instance, a polypeptide, protein (e.g., an PAOD1receptor), an agent that alters PAOD1 gene expression, or a PAOD1binding agent or binding partner, fragment, fusion protein or prodrugthereof, or a nucleotide or nucleic acid construct (vector) comprising anucleotide of the present invention, or an agent that alters PAOD1polypeptide activity, can be formulated with a physiologicallyacceptable carrier or excipient to prepare a pharmaceutical composition.The carrier and composition can be sterile. The formulation should suitthe mode of administration.

[0130] Suitable pharmaceutically acceptable carriers include but are notlimited to water, salt solutions (e.g., NaCl), saline, buffered saline,alcohols, glycerol, ethanol, gum arabic, vegetable oils, benzylalcohols, polyethylene glycols, gelatin, carbohydrates such as lactose,amylose or starch, dextrose, magnesium stearate, talc, silicic acid,viscous paraffin, perfume oil, fatty acid esters,hydroxymethylcellulose, polyvinyl pyrolidone, etc., as well ascombinations thereof. The pharmaceutical preparations can, if desired,be mixed with auxiliary agents, e.g., lubricants, preservatives,stabilizers, wetting agents, emulsifiers, salts for influencing osmoticpressure, buffers, coloring, flavoring and/or aromatic substances andthe like which do not deleteriously react with the active agents.

[0131] The composition, if desired, can also contain minor amounts ofwetting or emulsifying agents, or pH buffering agents. The compositioncan be a liquid solution, suspension, emulsion, tablet, pill, capsule,sustained release formulation, or powder. The composition can beformulated as a suppository, with traditional binders and carriers suchas triglycerides. Oral formulation can include standard carriers such aspharmaceutical grades of mannitol, lactose, starch, magnesium stearate,polyvinyl pyrollidone, sodium saccharine, cellulose, magnesiumcarbonate, etc.

[0132] Methods of introduction of these compositions include, but arenot limited to, intradermal, intramuscular, intraperitoneal,intraocular, intravenous, subcutaneous, topical, oral and intranasal.Other suitable methods of introduction can also include gene therapy (asdescribed below), rechargeable or biodegradable devices, particleacceleration devises (“gene guns”) and slow release polymeric devices.The pharmaceutical compositions of this invention can also beadministered as part of a combinatorial therapy with other agents.

[0133] The composition can be formulated in accordance with the routineprocedures as a pharmaceutical composition adapted for administration tohuman beings. For example, compositions for intravenous administrationtypically are solutions in sterile isotonic aqueous buffer. Wherenecessary, the composition may also include a solubilizing agent and alocal anesthetic to ease pain at the site of the injection. Generally,the ingredients are supplied either separately or mixed together in unitdosage form, for example, as a dry lyophilized powder or water freeconcentrate in a hermetically sealed container such as an ampule orsachette indicating the quantity of active agent. Where the compositionis to be administered by infusion, it can be dispensed with an infusionbottle containing sterile pharmaceutical grade water, saline ordextrose/water. Where the composition is administered by injection, anampule of sterile water for injection or saline can be provided so thatthe ingredients may be mixed prior to administration.

[0134] For topical application, nonsprayable forms, viscous tosemi-solid or solid forms comprising a carrier compatible with topicalapplication and having a dynamic viscosity preferably greater thanwater, can be employed. Suitable formulations include but are notlimited to solutions, suspensions, emulsions, creams, ointments,powders, enemas, lotions, sols, liniments, salves, aerosols, etc., whichare, if desired, sterilized or mixed with auxiliary agents, e.g.,preservatives, stabilizers, wetting agents, buffers or salts forinfluencing osmotic pressure, etc. The agent may be incorporated into acosmetic formulation. For topical application, also suitable aresprayable aerosol preparations wherein the active ingredient, preferablyin combination with a solid or liquid inert carrier material, ispackaged in a squeeze bottle or in admixture with a pressurizedvolatile, normally gaseous propellant, e.g., pressurized air.

[0135] Agents described herein can be formulated as neutral or saltforms. Pharmaceutically acceptable salts include those formed with freeamino groups such as those derived from hydrochloric, phosphoric,acetic, oxalic, tartaric acids, etc., and those formed with freecarboxyl groups such as those derived from sodium, potassium, ammonium,calcium, ferric hydroxides, isopropylamine, triethylamine, 2-ethylaminoethanol, histidine, procaine, etc.

[0136] The agents are administered in a therapeutically effectiveamount. The amount of agents which will be therapeutically effective inthe treatment of a particular disorder or condition will depend on thenature of the disorder or condition, and can be determined by standardclinical techniques. In addition, in vitro or in vivo assays mayoptionally be employed to help identify optimal dosage ranges. Theprecise dose to be employed in the formulation will also depend on theroute of administration, and the seriousness of the symptoms ofperipheral arterial occlusive disease, and should be decided accordingto the judgment of a practitioner and each patient's circumstances.Effective doses may be extrapolated from dose-response curves derivedfrom in vitro or animal model test systems.

[0137] The invention also provides a pharmaceutical pack or kitcomprising one or more containers filled with one or more of theingredients of the pharmaceutical compositions of the invention.Optionally associated with such container(s) can be a notice in the formprescribed by a governmental agency regulating the manufacture, use orsale of pharmaceuticals or biological products, which notice reflectsapproval by the agency of manufacture, use of sale for humanadministration. The pack or kit can be labeled with informationregarding mode of administration, sequence of drug administration (e.g.,separately, sequentially or concurrently), or the like. The pack or kitmay also include means for reminding the patient to take the therapy.The pack or kit can be a single unit dosage of the combination therapyor it can be a plurality of unit dosages. In particular, the agents canbe separated, mixed together in any combination, present in a singlevial or tablet. Agents assembled in a blister pack or other dispensingmeans is preferred. For the purpose of this invention, unit dosage isintended to mean a dosage that is dependent on the individualpharmacodynamics of each agent and administered in FDA approved dosagesin standard time courses.

[0138] Methods of Therapy

[0139] The present invention also pertains to methods of treatment(prophylactic and/or therapeutic) for peripheral arterial occlusivedisease or susceptibility to peripheral arterial occlusive disease,using a PAOD1 therapeutic agent. A “PAOD1 therapeutic agent” is an agentthat alters (e.g., enhances or inhibits) PAOD1 polypeptide activityand/or PAOD1 gene expression, as described herein (e.g., a PAOD1 agonistor antagonist). PAOD1 therapeutic agents can alter PAOD1 polypeptideactivity or gene expression by a variety of means, such as, for example,by providing additional PAOD1 polypeptide or by upregulating thetranscription or translation of the PAOD1 gene; by alteringposttranslational processing of the PAOD1 polypeptide; by alteringtranscription of PAOD1 splicing variants; or by interfering with PAOD1polypeptide activity (e.g., by binding to a PAOD1 polypeptide), or bydownregulating the transcription or translation of the PAOD1 gene.Representative PAOD1 therapeutic agents include the following: nucleicacids or fragments or derivatives thereof described herein, particularlynucleotides encoding the polypeptides described herein and vectorscomprising such nucleic acids (e.g., a gene, cDNA, and/or mRNA, such asa nucleic acid encoding a PAOD1 polypeptide or active fragment orderivative thereof, or an oligonucleotide; for example, SEQ ID NO: 1which may optionally comprise at least one polymorphism shown in Table 1or a nucleic acid encoding an isoform, or fragments or derivativesthereof); polypeptides described herein (e.g., one or more of SEQ IDNOs: 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, and/or other splicingvariants encoded by PAOD1, or fragments or derivatives thereof); otherpolypeptides (e.g., PAOD1 receptors); PAOD1 binding agents;peptidomimetics; fusion proteins or prodrugs thereof; antibodies (e.g.,an antibody to a mutant PAOD1 polypeptide, or an antibody to anon-mutant PAOD1 polypeptide, or an antibody to a particular splicingvariant encoded by PAOD1, as described above); ribozymes; other smallmolecules; and other agents that alter (e.g., enhance or inhibit) PAOD1gene expression or polypeptide activity, or that regulate transcriptionof PAOD1 splicing variants (e.g., agents that affect which splicingvariants are expressed, or that affect the amount of each splicingvariant that is expressed. More than one PAOD1 therapeutic agent can beused concurrently, if desired.

[0140] The PAOD1 therapeutic agent that is a nucleic acid is used in thetreatment of peripheral arterial occlusive disease or treatment for asusceptibility of peripheral arterial occlusive disease. The term,“treatment” as used herein, refers not only to ameliorating symptomsassociated with the disease, but also preventing or delaying the onsetof the disease, and also lessening the severity or frequency of symptomsof the disease. The therapy is designed to alter (e.g., inhibit orenhance), replace or supplement activity of a PAOD1 polypeptide in anindividual. For example, a PAOD1 therapeutic agent can be administeredin order to upregulate or increase the expression or availability of thePAOD1 gene or of specific splicing variants of PAOD1, or, conversely, todownregulate or decrease the expression or availability of the PAOD1gene or specific splicing variants of PAOD1. Upregulation or increasingexpression or availability of a native PAOD1 gene or of a particularsplicing variant could interfere with or compensate for the expressionor activity of a defective gene or another splicing variant;downregulation or decreasing expression or availability of a nativePAOD1 gene or of a particular splicing variant could minimize theexpression or activity of a defective gene or the particular splicingvariant and thereby minimize the impact of the defective gene or theparticular splicing variant.

[0141] The PAOD1 therapeutic agent(s) are administered in atherapeutically effective amount (i.e., an amount that is sufficient totreat the disease, such as by ameliorating symptoms associated with thedisease, preventing or delaying the onset of the disease, and/or alsolessening the severity or frequency of symptoms of the disease). Theamount which will be therapeutically effective in the treatment of aparticular individual's disorder or condition will depend on thesymptoms and severity of the disease, and can be determined by standardclinical techniques. In addition, in vitro or in vivo assays mayoptionally be employed to help identify optimal dosage ranges. Theprecise dose to be employed in the formulation will also depend on theroute of administration, and the seriousness of the disease or disorder,and should be decided according to the judgment of a practitioner andeach patient's circumstances. Effective doses may be extrapolated fromdose-response curves derived from in vitro or animal model test systems.

[0142] In one embodiment, a nucleic acid of the invention (e.g., anucleic acid encoding a PAOD1 polypeptide, such as SEQ ID NO: 1 whichmay optionally comprise at least one polymorphism shown in Table 1; oranother nucleic acid that encodes a PAOD1 polypeptide or a splicingvariant, derivative or fragment thereof, such as a nucleic acid encodingany one of SEQ ID NOs: 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36) canbe used, either alone or in a pharmaceutical composition as describedabove. For example, PAOD1 or a cDNA encoding the PAOD1 polypeptide,either by itself or included within a vector, can be introduced intocells (either in vitro or in vivo) such that the cells produce nativePAOD1 polypeptide. If necessary, cells that have been transformed withthe gene or cDNA or a vector comprising the gene or cDNA can beintroduced (or re-introduced) into an individual affected with thedisease. Thus, cells which, in nature, lack native PAOD1 expression andactivity, or have mutant PAOD1 expression and activity, or haveexpression of a disease-associated PAOD1 splicing variant, can beengineered to express PAOD1 polypeptide or an active fragment of thePAOD1 polypeptide (or a different variant of PAOD1 polypeptide). In apreferred embodiment, nucleic acid encoding the PAOD1 polypeptide, or anactive fragment or derivative thereof, can be introduced into anexpression vector, such as a viral vector, and the vector can beintroduced into appropriate cells in an animal. Other gene transfersystems, including viral and nonviral transfer systems, can be used.Alternatively, nonviral gene transfer methods, such as calcium phosphatecoprecipitation, mechanical techniques (e.g., microinjection); membranefusion-mediated transfer via liposomes; or direct DNA uptake, can alsobe used.

[0143] Alternatively, in another embodiment of the invention, a nucleicacid of the invention; a nucleic acid complementary to a nucleic acid ofthe invention; or a portion of such a nucleic acid (e.g., anoligonucleotide as described below), can be used in “antisense” therapy,in which a nucleic acid (e.g., an oligonucleotide) which specificallyhybridizes to the mRNA and/or genomic DNA of PAOD1 is administered orgenerated in situ. The antisense nucleic acid that specificallyhybridizes to the mRNA and/or DNA inhibits expression of the PAOD1polypeptide, e.g., by inhibiting translation and/or transcription.Binding of the antisense nucleic acid can be by conventional base paircomplementarity, or, for example, in the case of binding to DNAduplexes, through specific interaction in the major groove of the doublehelix.

[0144] An antisense construct of the present invention can be delivered,for example, as an expression plasmid as described above. When theplasmid is transcribed in the cell, it produces RNA which iscomplementary to a portion of the mRNA and/or DNA which encodes PAOD1polypeptide. Alternatively, the antisense construct can be anoligonucleotide probe which is generated ex vivo and introduced intocells; it then inhibits expression by hybridizing with the mRNA and/orgenomic DNA of PAOD1. In one embodiment, the oligonucleotide probes aremodified oligonucleotides which are resistant to endogenous nucleases,e.g. exonucleases and/or endonucleases, thereby rendering them stable invivo. Exemplary nucleic acid molecules for use as antisenseoligonucleotides are phosphoramidate, phosphothioate andmethylphosphonate analogs of DNA (see also U.S. Pat. Nos. 5,176,996;5,264,564; and 5,256,775). Additionally, general approaches toconstructing oligomers useful in antisense therapy are also described,for example, by Van der Krol et al. ((1988) Biotechniques 6:958-976);and Stein et al. ((1988) Cancer Res 48:2659-2668). With respect toantisense DNA, oligodeoxyribonucleotides derived from the translationinitiation site, e.g. between the −10 and +10 regions of PAOD1 sequence,are preferred.

[0145] To perform antisense therapy, oligonucleotides (mRNA, cDNA orDNA) are designed that are complementary to mRNA encoding PAOD1. Theantisense oligonucleotides bind to PAOD1 mRNA transcripts and preventtranslation. Absolute complementarity, although preferred, is notrequired a sequence “complementary” to a portion of an RNA, as referredto herein, indicates that a sequence has sufficient complementarity tobe able to hybridize with the RNA, forming a stable duplex; in the caseof double-stranded antisense nucleic acids, a single strand of theduplex DNA may thus be tested, or triplex formation may be assayed. Theability to hybridize will depend on both the degree of complementarityand the length of the antisense nucleic acid, as described in detailabove. Generally, the longer the hybridizing nucleic acid, the more basemismatches with an RNA it may contain and still form a stable duplex (ortriplex, as the case may be). One skilled in the art can ascertain atolerable degree of mismatch by use of standard procedures.

[0146] The oligonucleotides used in antisense therapy can be DNA, RNA,or chimeric mixtures or derivatives or modified versions thereof,single-stranded or double-stranded. The oligonucleotides can be modifiedat the base moiety, sugar moiety, or phosphate backbone, for example, toimprove stability of the molecule, hybridization, etc. Theoligonucleotides can include other appended groups such as peptides(e.g. for targeting host cell receptors in vivo), or agents facilitatingtransport across the cell membrane (see, e.g., Letsinger et al. (1989)Proc. Natl. Acad. Sci. USA 86:6553-6556; Lemaitre et al., (1987), Proc.Natl. Acad Sci. USA 84:648-652; PCT International Publication No.WO88/09810) or the blood-brain barrier (see, e.g., PCT InternationalPublication No. WO89/10134), or hybridization-triggered cleavage agents(see, e.g., Krol et al. (1988) BioTechniques 6:958-976) or intercalatingagents. (See, e.g., Zon, (1988), Pharm. Res. 5:539-549). To this end,the oligonucleotide may be conjugated to another molecule (e.g., apeptide, hybridization triggered cross-linking agent, transport agent,hybridization-triggered cleavage agent).

[0147] The antisense molecules are delivered to cells which expressPAOD1 in vivo. A number of methods can be used for delivering antisenseDNA or RNA to cells; e.g., antisense molecules can be injected directlyinto the tissue site, or modified antisense molecules, designed totarget the desired cells (e.g., antisense linked to peptides orantibodies that specifically bind receptors or antigens expressed on thetarget cell surface) can be administered systemically. Alternatively, ina preferred embodiment, a recombinant DNA construct is utilized in whichthe antisense oligonucleotide is placed under the control of a strongpromoter (e.g., pol III or pol II). The use of such a construct totransfect target cells in the patient results in the transcription ofsufficient amounts of single stranded RNAs that will form complementarybase pairs with the endogenous PAOD1 transcripts and thereby preventtranslation of the PAOD1 mRNA. For example, a vector can be introducedin vivo such that it is taken up by a cell and directs the transcriptionof an antisense RNA. Such a vector can remain episomal or becomechromosomally integrated, as long as it can be transcribed to producethe desired antisense RNA. Such vectors can be constructed byrecombinant DNA technology methods standard in the art and describedabove. For example, a plasmid, cosmid, YAC or viral vector can be usedto prepare the recombinant DNA construct which can be introduceddirectly into the tissue site. Alternatively, viral vectors can be usedwhich selectively infect the desired tissue, in which caseadministration may be accomplished by another route (e.g.,systemically).

[0148] Endogenous PAOD1 expression can also be reduced by inactivatingor “knocking out” PAOD1 or its promoter using targeted homologousrecombination (e.g., see Smithies et al. (1985) Nature 317:230-234;Thomas & Capecchi (1987) Cell 51:503-512; Thompson et al. (1989) Cell5:313-321). For example, a mutant, non-functional PAOD1 (or a completelyunrelated DNA sequence) flanked by DNA homologous to the endogenousPAOD1 (either the coding regions or regulatory regions of PAOD1) can beused, with or without a selectable marker and/or a negative selectablemarker, to transfect cells that express PAOD1 in vivo. Insertion of theDNA construct, via targeted homologous recombination, results ininactivation of PAOD1. The recombinant DNA constructs can be directlyadministered or targeted to the required site in vivo using appropriatevectors, as described above. Alternatively, expression of non-mutantPAOD1 can be increased using a similar method: targeted homologousrecombination can be used to insert a DNA construct comprising anon-mutant, functional PAOD1 (e.g., a gene having SEQ ID NO: 1 which mayoptionally comprise at least one polymorphism shown in Table 1), or aportion thereof, in place of a mutant PAOD1 in the cell, as describedabove. In another embodiment, targeted homologous recombination can beused to insert a DNA construct comprising a nucleic acid that encodes aPAOD1 polypeptide variant that differs from that present in the cell.

[0149] Alternatively, endogenous PAOD1 expression can be reduced bytargeting deoxyribonucleotide sequences complementary to the regulatoryregion of PAOD1 (i.e., the PAOD1 promoter and/or enhancers) to formtriple helical structures that prevent transcription of PAOD1 in targetcells in the body. (See generally, Helene, C. (1991) Anticancer DrugDes., 6(6):569-84; Helene, C., et al. (1992) Ann, N.Y. Acad. Sci.,660:27-36; and Maher, L. J. (1992) Bioassays 14(12):807-15). Likewise,the antisense constructs described herein, by antagonizing the normalbiological activity of one of the PAOD1 proteins, can be used in themanipulation of tissue, e.g. tissue differentiation, both in vivo and/orex vivo tissue cultures. Furthermore, the anti-sense techniques (e.g.microinjection of antisense molecules, or transfection with plasmidswhose transcripts are anti-sense with regard to a PAOD1 mRNA or genesequence) can be used to investigate role of PAOD1 in developmentalevents, as well as the normal cellular function of PAOD1 in adulttissue: Such techniques can be utilized in cell culture, but can also beused in the creation of transgenic animals.

[0150] In yet another embodiment of the invention, other PAOD1therapeutic agents as described herein can also be used in the treatmentor prevention of peripheral arterial occlusive disease. The therapeuticagents can be delivered in a composition, as described above, or bythemselves. They can be administered systemically, or can be targeted toa particular tissue. The therapeutic agents can be produced by a varietyof means, including chemical synthesis; recombinant production; in vivoproduction (e.g., a transgenic animal, such as U.S. Pat. No. 4,873,316to Meade et al.), for example, and can be isolated using standard meanssuch as those described herein.

[0151] A combination of any of the above methods of treatment (e.g.,administration of non-mutant PAOD1 polypeptide in conjunction withantisense therapy targeting mutant PAOD1 mRNA; administration of a firstsplicing variant encoded by PAOD1 in conjunction with antisense therapytargeting a second splicing encoded by PAOD1), can also be used.

[0152] The invention will be further described by the followingnon-limiting examples. The teachings of all publications cited hereinare incorporated herein by reference in their entirety.

EXAMPLES Example 1 Identification of the PAOD1 Gene

[0153] Materials and Methods

[0154] Population and Genealogy

[0155] This study was approved by the Data Protection Commission ofIceland and the National Bioethics Committee of Iceland. Informedconsent was obtained from all patients and their relatives whose DNAsamples were used in the linkage scan. The original population-basedlists of PAOD1 patients covering the years 1980 to 1995 were derivedfrom the two major hospitals in Iceland. All patients had undergoneangiography and 85% had angioplasty and/or vascular surgery. Thoseundergoing vascular procedures for trauma were excluded.

[0156] A comprehensive genealogy database that has been established atdeCODE genetics, Inc. was used to cluster the patients in pedigrees(Gulcher, J. R., and Stefansson, K., Clin. Chem. Lab. Med. 36:523(1998)). Each version of the computerized genealogy database isreversibly encrypted by the Data Protection Commission of Iceland beforearriving at the laboratory (Gulcher, J. R., et al., Eur. J. Hum. Genet.8:739 (2000)). The database uses a patient list, with encrypted personalidentifiers, as input, and recursive algorithms to find all ancestors inthe database who are related to any member on the input list within agiven number of generations back. The cluster function then searches forancestors who are common to any two or more members of the input list.

[0157] Microsatellite Markers and Genotyping

[0158] The marker order and positions for the framework mapping set wereobtained from the Marshfield genetic map except for a three-markerputative inversion on chromosome 8 (Jonsdottir, G. M. et al., Am. J.Hum. Genet. 67:332 (2000); Yu, A. et al., Am. J. Hum. Genet. 67:10(2000); Giglio et al., Am. J. Hum. Genet., 68:874-83 (2001)).

[0159] The DNA samples were genotyped using nearly 1000 fluorescentlylabelled primers. A microsatellite screening set was developed based inpart on the ABI Linkage Marker (v2) screening set and the ABI LinkageMarker (v2) intercalating set in combination with over 500 custom-mademarkers. All markers were extensively tested for robustness, ease ofscoring, and efficiency in 4× multiplex PCR reactions. The PCRamplifications were set up, run and pooled on Perkin Elmer/AppliedBiosystems 877 Integrated Catalyst Thermocyclers with a similar protocolfor each marker. The reaction volume used was 5 μl and for each PCRreaction 20 ng of genomic DNA was amplified in the presence of 2 μmol ofeach primer, 0.25 U AmpliTaq Gold, 0.2 mM dNTPs and 2.5 mM MgCl₂ (bufferwas supplied by manufacturer). The PCR conditions used were 95° C. for10 minutes, then 37 cycles of 15 s at 94° C., 30 s at 55° C. and 1 minat 72° C. The PCR products were supplemented with the internal sizestandard and the pools were separated and detected on Applied Biosystemsmodel 377 Sequencer using GENESCAN v3.0 peak calling software. Alleleswere called automatically with the TrueAllele program, which performsautomated allele calling on DNA fragment data from automated fluorescentsequencers. The software automatically tracks lanes, and assesses thequality of every genotype it calls. TrueAllele uses stutterdeconvolution to mathematically remove PCR stutter (“shadow bands”) fromthe data. See, e.g., U.S. Pat. Nos. 5,541,067, 5,580,728, 5,876,933, and6,054,268. Alleles were also called automatically with the programDecodeGT (deCODE genetics, Iceland), which was used to fractionateaccording to quality and edit the called genotypes (Palsson, B. et al.,Genome Res. 9:1002-12 (1999)). At least 180 Icelandic controls weregenotyped to derive allelic frequencies.

[0160] Decode-GT is an editing program that works downstream of theallele calling program, TrueAllele (Cybergenetics, Inc.). It is aparametric approach to allele call quality control that eliminates muchof the time required for manual editing of the data. Decode-GT is a PCprogram that runs under Windows NT and has three main functions. First,it sorts the allele calls according to quality measures and can displaythe electropherograms on which they are based. Second, it checks theallele calls of CEPH control samples to ensure that the gel is properlycalibrated. Third, it performs an inheritance check on the results usingpedigree information. Decode-GT reads the combined results file fromTrueAllele and sorts the data into three categories—bad allele calls,good allele calls, and ambiguous allele calls—sorting is based on aTrueAllele quality measure, the peak heights, and the peak shifts. Theaim is that only calls in the ambiguous category need be inspected bythe user, thereby reducing dependence on manual editing.

[0161] Statistical Methods for Linkage Analysis

[0162] In the analyses described herein, multipoint, affected-onlyallele-sharing methods were used to assess the evidence for linkage. Allrelatives who did not have an angiogram or surgery for PAOD1 wereconsidered to have “unknown” status. All results were obtained using theprogram ALLEGRO (Gudbjartsson, D. F. et al., Nat. Genet. 25:12-3(2000)). The S_(pairs) scoring function (Whittemore, A. S. and Halpern,J., Biometrics 50:118-27 (1994); Kruglyak, L. et al., Am. J. Hum. Genet.58:1347-63 (1996)) and the exponential allele-sharing model (Kong, A.and Cox, N. J., Am. J. Hum. Genet. 61:1179-88 (1997)) were used togenerate the relevant one degree of freedom statistics. When combiningthe family scores to obtain an overall score, instead of weighting thefamilies equally, the default of GENEHUNTER (Kruglyak, L. et al., Am. J.Hum. Genet. 58:1347-63 (1996)), or weighting the affected pairs equally,a weighting scheme was used which is half way between the two in the logscale; family weights are the geometric means of the weights of the twoschemes. While not identical, this weighting scheme tends to givesimilar results compared to that proposed by Weeks and Lange (Week, D.E. and Lange, K., Am. J. Hum. Genet. 42:315-26 (1988)) as an extensionof a weighting scheme of Hodge (Hodge, S. E., Genet. Epidemiol. 1:109-22(1984)) designed for sibships. The P value was computed two differentways and the less significant one was reported. The first P value wascomputed based on large sample theory; Z_(lr)=v(2 log_(e) (10)lod) isapproximately distributed as a standard normal random variable under thenull hypothesis of no linkage (Kong, A. and Cox, N. J., Am. J. Hum.Genet. 61:1179-88 (1997)). Because of the concern with small samplebehavior, a second P value was computed by comparing the observed lodscore to its complete data sampling distribution under the nullhypothesis (Gudbjartsson, D. F. et al., Nat. Genet. 25:12-3 (2000)).When a data set consists of more than a handful of families, which isthe case here, these two P values tend to be very similar. To ensurethat the result was a true reflection of the information contained inthe material, and to be considered a linkage result significant, notonly was it required that the P value be smaller than 2×10⁻⁵ (Lander, E.and Kruglyak, L., Nat. Genet. 11:241-7 (1995)), but also that theinformation content in the region was at least 85%. For the families inthis study, an information content of 85% corresponded to a markerdensity of approximately one marker every centimorgan. The informationmeasure used herein has been defined previously (Nicolae, D. L., 1999,Allele sharing models in gene mapping: a likelihood approach. Adissertation submitted to the faculty of the division of the physicalsciences in candidacy for the degree of doctor of philosophy, Universityof Chicago, Chicago) and implemented in ALLEGRO. This measure is closelyrelated to a classical measure of information (Dempster, A. P. et al.,J. Roy. Statist. Soc. B39:1-38 (1977)), having the property that it isbetween zero, if the marker genotypes are completely uninformative, andone, if the genotypes determine the exact amount of allele sharing bydescent among the affected relatives.

[0163] ALLEGRO allows exact multipoint linkage calculations involvingmany markers for general families, and is based on the programGENEHUNTER (Kruglyak, L., Daly, M. J., Reeve-Daly, M. P., Lander, E. S.(1996) Am. J. Hum. Genet. 58:1347-1363.), and its later modification,GENEHUNTER-PLUS. Like GENEHUNTER, ALLEGRO uses a hidden Markov model,but it is considerably faster than Genehunter (typically 20-100 times).Apart from allowing for larger pedigrees (typically 20-30% larger), thespeed improvement is relevant for simulation studies. ALLEGRO has muchof the functionality of GENEHUNTER and GENEHUNTER-PLUS. Specifically,ALLEGRO calculates multipoint parametric lod scores, NPL scores andallele-sharing lod scores based on the scoring functions S_(pairs) andS_(all), reconstruction of haplotypes, estimated recombination countbetween markers (observed map), and entropy information. The Xchromosome is supported in all calculations.

[0164] In addition to S_(pairs) and S_(all), and the entropy measure,ALLEGRO supports other scoring functions and information measures. Otheradvantages are improved input and output, and the ability to performmultiple analyses together at little extra cost (with differentparametric models, scoring functions and family-weighting schemes).Also, at a cost of 10-30% in run time, ALLEGRO will, if necessary, userecalculation or disk-swapping to cut down memory requirements by afactor of 20-60 compared with GENEHUNTER. ALLEGRO can simulatemulti-locus marker data either under no linkage or under linkage.Simulations under no linkage can be used to study the calibration ofdifferent methods of calculating P values. One can determine genome-wideadjusted P values for specific families and marker density. Underlinkage to a susceptibility gene, ALLEGRO will simulate marker dataconditional on the observed disease phenotypes and a given inheritancemode. These simulations can be used to assess the power of a set offamilies, or the effects of marker density and missing data. They canalso be used to compare parametric and non-parametric methods, variousscoring functions and weighting schemes, and SNPs versusmicrosatellites.

[0165] After obtaining a significant allele-sharing lod score, in anattempt to understand the contribution of this susceptibility locus, arange of parametric models was fitted to the data. Even when fittingparametric models, affected-only analyses were performed in the sensethat an individual is either classified as affected or having unknowndisease status. As a consequence, only ratios of penetrances arerelevant. A range of single locus dominant, additive and multiplicativemodels (Risch, N., Am. J. Hum. Genet. 46:229-41 (1990)) was fitted. Witha complex disease like PAOD1, none of these simple models are likely tobe exactly true and the effect of a gene and its variants can only bereliably determined after the at-risk variant, or variants, areidentified. However, by calculating the corresponding contribution tothe sibling recurrence risk ratio, the fitted parametric models doprovide some rough idea as to how much the gene is contributing to thefamilial clustering of the disease.

[0166] In order to assess whether the increase in lod score resultingfrom subtracting the 35 PAOD1 patients who also had stroke would belikely to occur by chance, 1000 random sets of 35 patients whose statuswas then changed to “unknown” in an analysis were selected. The P valueused is the fraction of the 1000 simulations which produced a lod scoreincrease at the peak locus equal to or greater than that which wasobserved by changing the affection status of the stroke patients to“unknown”. The same method was used to assess subtracting the 38 PAOD1patients who also had MI. There, however, the significance of thedecrease in lod score was assessed by looking at the fraction of thesimulations which produced a lod score decrease greater or equal to thatwhich was observed by changing the affection status of the MI patientsto “unknown”.

[0167] Physical and Genetic Mapping

[0168] For the locus region, a combination of data from coincidenthybridizations of BAC membranes using a high density of STSs and theFinger Printing Contig (FPC) database was used to build large contigs ofBACs from the RPCI-11 library provided by Pieter deJong (Childrens'sHospital Oakland Research Institute). BAC contigs were generated by amethod that combines coincident hybridization versus the RPCI-11 BAClibrary together with data mining and the FPC program. Hybridizationswere performed using primers from markers in the region of interestaccording to their location in the Weizmann Institute Unified Database.To close the gaps, new markers were generated from BAC end sequences.

[0169] High-resolution genetic mapping was used both to anchor and placein order contigs found by physical mapping as well as to obtain accurateinter-marker distances for the correctly ordered markers (See Table 2for marker orders and distances used). Data from 112 Icelandic nuclearfamilies (sibships with their parents, containing from two to sevensiblings) were analyzed together with the nuclear families availablewithin the PAOD1 pedigrees. For the purpose of genetic mapping the 112nuclear families alone provided 588 meioses, and the total number ofmeioses available for mapping was over 1000. By comparison, theMarshfield genetic map was constructed based on 182 meloses. The largenumber of meiotic events within our families provides the ability to mapmarkers to a resolution of 1.0 cM or better. Using a modification of theALLEGRO program, the EM algorithm (Dempster, A. P. et al., J. Roy.Statist. Soc. B39:1-38 (1977)) was applied to the data to estimate thegenetic distances between markers. Combining this information with thephysical map resulted in a highly reliable order of markers and betterestimates of inter-marker distances within the peak region, both ofwhich are important for an accurate linkage analysis (Halpern, J. andWhittemore, A. S., Hum. Hered. 49:194-6 (1999); Daw, E. W. et al.,Genet. Epidemiol. 19:366-80 (2000)).

[0170] The PAOD1 Gene

[0171] The PAOD1 human genomic sequence (SEQ ID NO: 1) is set forth inFIG. 4 and comprises 322,101 nts (partially described in GenbankAccession Nos. >gi|4775605|emb|AL031429.11|HS333A15and >gi|16972792|emb|AL158087.12|AS158087; entire teachings incorporatedherein). This sequence is representative of a healthy individual. TheORF starts at ATG at nucleotide 58,162 of exon 1. There are twelve exonsshown, exons 4 and 5 are new. The gene encodes a GPCR, prostaglandin Ereceptor 3 (subtype 3) protein, which is alternatively expressed. FIG. 3shows 11 isoforms; EP3g and EP3h are newly reported herein. Exons 1 and2 are found in all PTGER3 isoforms and code for most of the protein,i.e., the 359 amino acids that make up the extracellular domain and all7 transmembrane spanning helices. The tail-forming exons make up thedifferent PTGER3 isoforms.

[0172] Single nucleotide polymorphisms (SNPs) are listed in Table 1. Forknown SNPs, the GenBank Accession numbers is provided. The nucleotidenumbering is relative to SEQ ID NO: 1. Four additional SNPs have beenidentified and are identified with the “mut” suffix. In PAOD1 patientversus control studies, two SNPs showed good p-values, as follows:rs571705 p-0.0063 d15ptgSNP697624 p-0.00466

[0173] Based upon this, one or a combination of these SNPs can be usedto diagnose PAOD1 or predisposition thereto.

[0174] Prostaglandin E receptor 3 protein is widely distributed intissue, including smooth muscle cells, platelets, endothelium andmacrophages. They are know to bind to G proteins, including Gi/Go, Gsand Gp. See Namba et al., Nature 365: 166-170 (1993).

[0175] Results

[0176] A genealogy database, together with a population-based list of1745 patients who had undergone angiography and/or surgery for PAOD1,was used to define families of PAOD1 patients. The study describedherein was focused on patients who were related to other patients within6 meiotic events (a total of 272 patients and 612 relatives within 116families). FIG. 1 exhibits two of the larger families used in theanalysis as well as an example of a family in which a PAOD1 patient isrelated to another PAOD1 patient who also had stroke. The most prominentlinkage was found to chromosome 1p with a lod score of 2.95, althoughother lod scores above 1.5 also occur on 13q and 18q. Additionalmicrosatellite markers were genotyped and mapped for the chromosome 1ppeak region. Using the genome map annotated at deCODE, the multipointanalysis increased the lod score on chromosome 1 to 3.93 (P=1.04·10⁻⁵)(FIG. 2). The peak is centered on marker Dl S2895, with markers D1S411and D1S2855, telomeric and centromeric, respectively, defining a drop ofone in lod score from the peak. This locus was designated as PAOD11. ThePAOD1 patients in the families that contributed to the linkage did nothave a higher rate of diabetes, hypertension, or hyperlipidemia than thefamilies not contributing to the locus, suggesting that this locus mightnot be a locus for these risk factors.

[0177] Our genetic map for 19 markers in the peak region, along with thepublicly available Marshfield genetic map, is displayed in Table 2. TheMarshfield map differs from our map in both the order of a number ofmarkers for which Marshfield has no resolution and in the geneticdistance between markers. When our data is analyzed using the Marshfieldmap directly, the lod score at the peak is 2.83. Using the correct orderfor the markers, but the Marshfield distances, the lod score is also2.83. At the time of our submission the December 2000 freeze of the UCSCdraft assembly of the human DNA sequence was available. Our order ofmarkers agreed with the public sequence except for the two markers whichare indicated in Table 2. The April 2001 freeze (released June 2001)corrected the order of these two markers, but changed the orders of twoother pairs (not shown in Table 2). The August 2001 freeze agrees withthe order we have.

[0178] Some demographic information as well as the fraction of patientswith several risk factors is given in Table 3 for the entire patientset. For the risk factors, we have also displayed the fractions for theset of patients in families with NPL scores of one or more (the patientsin these families show an excess of sharing genetic material identicalby descent over what would be expected simply due to theirrelationship). There does not appear to be any substantial shifting inthe pattern of risk factors for the patients from these families. Thissuggests that this PAOD1 locus is not a locus for these risk factors.

[0179] PAOD1 is often associated with other forms of atherosclerosis,such as cerebrovascular disease and coronary artery disease, and many ofour patients also had a history of stroke and/or myocardial infarction.When the data was reanalyzed after defining those 35 PAOD1 patients (13%of total) who also had history of stroke, as having unknown diseasestatus, instead of as affected, the led score on chromosome 1 increasedto 4.93. This increase in the lod score due to subtraction of the strokepatients is statistically significant (P=0.019) (FIG. 2). Subtraction ofthe 38 PAOD1 patients who also had MI (14%; designating them as“unknown”), resulted in a decrease in the lod score to 2.95. However,this decrease was not statistically significant (P=0.577) and may simplyreflect the reduction of the material.

[0180] A variety of parametric models, dominant, additive ormultiplicative were fitted to this locus. For each of these models itwas possible to achieve a lod score over 4.0 at this locus. For example,a dominant model which assumes that the at-risk allele has a frequencyof 14% and the penetrance of a non-carrier is zero gives a lod score of4.26. When fitting the dominant model, 47 out of the 116 families give apositive lod score; of these 38 families have lod scores above 0.1, andthree families have lod scores between 0.4 and 0.7. The three families,A, B and C, in FIG. 1 gave lod scores of 0.68, 0.62 and −0.12respectively, for this model. Note that, even for a family with anegative lod score, some or all of the patients can carry the at-riskallele since they could have inherited it from different sources. Amultiplicative model that assumes the at-risk allele has a frequency of20% and that there is a 7-fold increase in risk for every at-risk allelecarried gives a led score of 4.23. These dominant and multiplicativemodels correspond to sibling recurrence risk ratios of 2.37 and 2.54.

[0181] Discussion

[0182] In this study of PAOD1, the phenotype was defined as surgicallycorrected PAOD1 and/or angiographically documented which has manyadvantages. This decreased the subjectivity sometimes encountered whendefining PAOD1 based on the patient's history of intermittentclaudication. This also eliminated the need of relying on theankle-brachial blood pressure ratio that may vary from observer toobserver (Jeelani, N. U. et al., Eur. J. Vasc. Endovasc. Surg. 20:25-8(2000)). Since only about 20 to 25% of PAOD1 patients ever go on to haveangiography and surgery, this group represented those who are moreseverely affected by the disease.

[0183] The lack of prominent linkage to PAOD11 to stroke linkage studiessuggests that the PAOD1 gene confers a specific predisposition to PAOD1rather than to stroke or to PAOD1 in those patients with stroke. Thisincrease in lod score with the removal of stroke patients suggests thatthere may be other strong genetic and/or environmental factors thatcontribute to both PAOD1 and stroke.

[0184] This study suggests that there is both overlap and independencebetween genetic factors for the major manifestations of atherosclerosis,specifically PAOD1 and stroke. Since PAOD11 is unlikely to be simply ahypertension, diabetes or hyperlipidemia locus, this suggests thatadditional genetic factors may affect the pathogenesis ofatherosclerosis, either directly, in the arterial wall where it takesplace, or indirectly, through mediators in the blood, such asmononuclear cells, macrophages, platelets or coagulation factors. TABLE1 NA Position Relative to Amino GenBank Accession SEQ. ID Acid No. No: 1Change rs880099 50656 rs880098 51319 d15ptgSNP372803mu 58777 K (G/T)rs1005749 59824 rs1005748 59904 rs1005747 59907 rs2050065 61003rs1022530 81135 rs1022529 81280 rs1022528 81300 rs1022527 81448rs2179412 82009 rs2143157 82143 rs2206346 84650 rs2206345 84720 rs99799785220 rs1008484 85767 rs1569593 86008 rs2206344 90773 rs1474663 92830d15ptgSNP407605mu 93581 W (T/A) rs5680 94107 rs1983588 102929 rs1983587102970 rs1883461 112374 rs1883460 112564 rs2050066 116287 rs2223501122643 rs647921 123629 rs646621 123903 rs646511 123977 rs1409164 125519rs516647 127273 rs590222 127333 rs879424 127430 rs726764 128023 rs909848128272 rs909847 128295 rs873658 128380 rs508513 128451 rs484675 128685rs484538 128739 rs481940 128965 rs479934 129216 rs847735 133322 rs847734133330 rs653699 136921 rs596829 140456 rs594454 140954 rs594095 141031rs573688 144853 rs977214 145162 rs661000 149858 rs571705 151923 R (G/A)rs681196 154249 rs1071020 154700 rs650194 156559 rs500647 157924rs1409984 157982 rs499641 158040 rs622721 158098 rs847704 160082rs475468 164731 rs1327459 164891 rs570021 126032 rs541667 126788rs601934 127067 rs493489 176240 rs645618 176964 rs545983 177931 rs602383179776 rs2225026 182241 rs1854147 183835 rs942979 197085 rs1327466197314 rs875727 197683 rs942976 203851 rs942977 203958 rs942978 204229rs1327464 205184 rs1409989 205650 rs1359835 205928 rs1359834 206057rs1409165 207317 rs1409166 207541 rs1325948 207601 rs2068651 208554rs2068652 208578 rs1409977 218055 rs1409978 218318 rs1409981 218552rs510414 169694 rs625617 172008 rs496216 175953 rs578096 176739d15ptgSNP690878mut 243243 M (C/A) rs5703 243338 rs1327460 246377 rs959253160 rs766433 257197 rs2182324 262299 rs1327461 264672 rs1327462265905 rs1327463 275747 rs2182707 276879 rs2031750 278230 rs1409987278567 rs1409988 279596 rs1327453 286548 rs1327452 286855 rs1327451286942 rs1327450 286948 rs1327456 296563 rs1409976 297783 rs1327455297999 rs1327454 298032 rs2225110 298214 rs1536535 314267 rs1536261233025 rs1327449 233497 rs1325949 234229 rs1409985 237149 rs1409986239923 d15ptgSNP687624mu 239991 Y (C/T) rs1536534 315254 rs2209748319665 rs2209747 319670 rs1409980 320253 rs1409979 320291 rs943525321337

[0185] TABLE 2 Comparison of marker order and genetic distancesMarshfield deCODE genetics Genetic Marker Marker Genetic distance (cM)map map distance (cM) 96.04 D1S438 D1S438 91.808 97.49 D1S515 D1S51592.797 98.21 D1S2684 D1S2684 93.257 98.21 D1S2866 D1S2866 94.964 99.30D1S198 D1S198 95.980 100.39 D1S2829^(a) D1S2806 97.783

100.39 D1S2806 D1S2829 97.788 101.48 D1S411 D1S2803 99.120

101.48 D1S2803 D1S411 100.099 102.02 D1S2895 D1S2895 102.020 104.23D1S464 D1S464^(b) 104.358 104.23 D1S2855 D1S481^(b) 104.363

104.23 D1S481 D1S2855 104.368 105.45 D1S2876 D1S2876 107.276 106.45D1S2618 D1S2841 107.775

106.45 D1S2841 D1S2618 108.378 107.56 D1S500 D1S500 108.644 107.56D1S465 D1S465 109.234 109.04 D1S430 D1S430 109.840

[0186] TABLE 3 Demographics and risk factors of patients in the studyMean Sex (n = 272) All (n = 272) NPL > 1 (n = 75) age (yrs) Male (%)Female (%) Risk factor^(a) (%) (%) 70.8 169 (62) 103 (38) Hyperlipidemia33.8 39.2 Hypertension 51.8 48.6 Diabetes 9.9 9.5 Smoking^(b) 77.6 85

[0187] TABLE 4 Features of FIG. 4 CDS 58162-59061 CDS 93255-93437misc_feature: internal splice site 93435-93436 CDS 93435-93524 3′UTR93525-94233 CDS 94357-94581 3′UTR 94579-95040 misc_feature: splice site94281-94282 misc_feature: splice site 94355-94356 CDS 98112-98258 3′UTR98259-99051 CDS 131351-131446 3′UTR 131444-132260 CDS 131351-131442,134006-134012 3′UTR 134006-134734 CDS 151950-151979 CDS 152680-1527573′UTR 152758-153754 CDS 236419-236497 CDS 239904-240051 CDS243340-243368 3′UTR 243366-244167 CDS 252878-252898 3′UTR 252896-253623

[0188] While this invention has been particularly shown and describedwith reference to preferred embodiments thereof, it will be understoodby those skilled in the art that various changes in form and details maybe made therein without departing from the spirit and scope of theinvention as defined by the appended claims.

1 36 1 322101 DNA Homo sapiens misc_feature (1)...(322101) n = A,T,C orG 1 tgtgacatat ttaaataagt atgtgacatg gttgaatatg gagaggccca ctatttagcc 60aggaagtaaa ctttgacaaa acatccaagt aattcagtaa cttcatagtc acatcatttt 120tcagggattt aaatgtatat gtgtttggct tttctgattc atgtcattac tctttcaatg 180atagaatgga catggtacat tgcgctgaga ggaatacctc attacctttc acgttctctg 240aattgcacag tgttgcctaa aaatatcttt tcaccagaag agcctagaac agcactcaaa 300agagagccgt gtttgccctt acactgatct tagtcagtag acaccaatgg attattgagc 360tgcatgctgc tcataaatca acacatatta tgcttatatt gtcctgcatt ttagtctttc 420atctatgtac taagggacat ttaatgttaa actaatgtaa gaattgtctt ttatctgtat 480ggtttgtttc ggattatttt ctttagtata tttaccaatg accaacctct tcaaaacttc 540atacatactg atgatctatg taacgtatcc agggaaataa ttttatttct ctcaagaaaa 600aagttacata ttcaatgaat caatattcag actatgatga aaatcaactt tttgacaaca 660taatcatgct gtagttgaag attttattgg tgccacaaag aagtcaattc taaaatagat 720ctgtaataga agctcactgg ctattcacct gaactctgtt cacataggag gtaatattgg 780ttcaagtatt attaaagcac atgaaaagat aaaaagtaag ccttatgaca taaaattaat 840tccagaaaat tgactagttc taggtgatac attaacctga taattttgag aatttcagtt 900gttgtctaat gctaaggtat atgaactcga tagacatata attcttacgt ggcaagaata 960tagctcctaa ttaacatttg agggataaca ggaaattcta caccagatac caaagggata 1020acaggaaaat ctacaccaga tacagataag caggatggag gcactagcta atagcgctga 1080acctaggatt agaagatcag atttgatctt acacaaacca gtgactgtga tactacatat 1140gtctttgagc ccacatttct tgatctttaa aatgaaagca aaaacacctc ttttacaggg 1200tggtggagac agtaatggga aataatagat gtagaaatgc tttgtaaatt ctaaagtggt 1260ctagaaatat aagatactat ctcttctgtt tcttgttagt gccatcccgt aagctatcaa 1320ggcatagtaa taatcctgga aagcacaaat ttaaggcaaa acatttgtca aagaaacctg 1380attggaagaa gcaatataaa caagtttaat gaacagtctt tcctatgttt ccggatacaa 1440tggtaagtaa aatattacca cagatattaa ataaaagctt ctattaaaaa ggctgtgcta 1500aaatgcacac agagcctaag agatccattc cataggaatt catacagtca agcctagcaa 1560tgtctgaact gttatccaag caaactgtct caaaaattaa tcaattgata aaaaaattaa 1620tcaattggaa ttactatcac aacaccaaca caggtgaagg tagtggcatg ctgttcctgg 1680ggtatctaca tgtctgctag atacctgtta atatccagtt aaataaataa taggctgcaa 1740aaatggaaaa agccattgct tatgtcagtg atagaaaaaa aaatgtctag aaataaggaa 1800agaattagtg ataagttgag cacatggaat agctaccact ttaaaagcta tgtggactta 1860ggcttcccag aagtcccaga cctgtcattc tcctagctac tatcactcct tacaccaaag 1920ccgtcaggtt tgaataatag aaaagctctg gataccgagc tcactgagag ggaggcaatt 1980taactatccc tccatagtcc cggttttttc ttccctgaca gctgcaatgt cttccgagaa 2040tgaccttcca tagcaatgat gaaaaatgag agttgaagaa aaagattgtc acaaattcaa 2100actaaaggat tggtaaaata gtttaataag gtaagcgata taattttaga attgaaaacc 2160atatactcca aaccagaatt ttcttctaag cctcctttag ttggttaaga ctctaccatt 2220cttggcagtt acttaagctc aagtatattt gatcaccctt attataatct ttaaattcta 2280caaaataaca gtcacttgga taaattgtgg tatatttata tactagaata tcatcaaaca 2340atgaaaataa gtgagccaca gatacattca acagcatgta tgagtctcat caacattgtt 2400aggtgaaaga agttagacat aaaataatat attttatgta atgacaggta tatagaatta 2460aaaaatagtc actcctggtc tacggtgtta gaggtcagtg gagtttcctt gagagagaaa 2520aaaaggtatg ttctttgaaa gggctgaatg ggctttctga tatgttggaa atgttttatt 2580ttgtgatctg ggtagtgatt tcataggagc attaactttg taataatcta tgaaatggac 2640acttatcttt tatgcacttt tctgcacttt aaaaaattat gtaagttctt agtttggcct 2700ttaaggttga ccatgattat ttcccaatgt gttactccaa cctcaccctc aagttgcctc 2760cctaaatatt ttattccaac tatgttgctt tattcatgca tctcaaacaa ggttgtatta 2820ttttttaacc ctgttctttt gcttttgctg tttctaacta gaagtcatct tctgtctctt 2880catatcaaaa tcccaaccat ttttcagagc tcaactatac gtcttttatg tagccttccc 2940tcatttcagc aaatgttctt tctccatttt ctgagttgca acgatcattt gaacctctct 3000catcaatttg attttaatta cccctgaatt aaaaataagt tcatttgttt tattatgtaa 3060caggtatata aattattgga gagctgggaa ctatatacta catatcttga aattcttttc 3120cttacttcac agattagctg tacctggtgc taagtggata attgaatgag tctgtgaata 3180aataagcaaa taagccactg agtcaattat ttataaacat ctataattat tatacacctc 3240tgaggggccc ttggatcaag tttcatccca gcccatgaat aagtgttggt aagtcctcca 3300gtgtcctctt ggttctggcc aacaaatcag tagatgagtg gtggaaagtt atacaatatg 3360ttgtacctta ctacacccca aaagtgagag agaaatagac tgaaagtcta gaatcagtaa 3420atatactacc tgcccaagac aaaattgtga aatgagaagt tgatccttct ctacctttcc 3480tccaaggatc atgcctttgt ccaatcccac ctccacactg tctttctcct cttcctcctt 3540tattttcaga ggtcaatagt gacatctgtt gattgctgta ttcaagttct ctaaagaaac 3600agagccaaga ggatatgcgt atgcaaattt ccatgtcaag ggcaaagcaa gaaggttatt 3660gcaggatcta ctctgcaagg ttgccaaagg cctatgttct tggcacatga ctgaggcaaa 3720gcttcaccag atgtccttat acccacacta ctgccttaag ggatggcagg cagcctgaca 3780cctttttcct gaaatcagca tcctctgctg agaaaggtta acataggtct cactttaaag 3840gagaaatact aaaaggaatt gtctattatc atagaatata tattgaaggg tgaatttgga 3900gccaagaggc aataacttga taactggcat ataggctatg agtatcagtt atgagagcca 3960ataaattccc ttttggtttt agatcttttc agctggattt tctgaatatt gtacagaaag 4020actgttaact gacaaaaggc ccagtcctca taccacctag agcaaagcag agtttttcgt 4080tgaatattct tttctgagtc tttctgccac tattttccct caaaatcttt ctgccctggt 4140cctgtttttt ttagttagaa ttctacatat tgtctttgat atatacgtta aaagcctgtc 4200aacaacatat ttttatttta ataatagaat tatagaaaat gaaggcttta aaacagaaga 4260ttgatagagt taggtatttt taaaagttaa tagaatatct taggacaaca ggcactttgg 4320tttccttcaa ataattaagc tataatctat aataacaaaa catagttaga atttttaatt 4380caattaggat tgctctcaaa tctgcctggt aattctagga ttgcaccttg acaacaaaac 4440atggtcccaa aagaaagcat ttattttcag taaaaaataa aaatacctaa gtaagcaact 4500ttaatagggt ttacatgagt ttcagttaaa agtaagatat ctaatgcaaa agacaattgc 4560tttctgtgtt atttttaaaa acagaagtgc caaagagtag aggaattaag aaagtaaaaa 4620ctttacacaa tggtaaaaaa tgtgttagtg gctaaaaaaa ttactttatg aagtagactc 4680ccaaggaatg tataaaagtt tccaattaga caatattggt tctttgaaaa gtttcatttt 4740aagatttaga ctaacatata tcttttttta ttcttgactt cagcaatttc ttctaactgc 4800ctcagtagtc ttgtcttcct tgggttttat aaccacaaag tttattatta aaaagtttta 4860gttcacctca aatactttct gtgttatttg gtaaaagtat ctaggggaaa attcacgtaa 4920gaagagtaaa cccaatttaa cagaaaacat gaaaaattgt tgatgaaggg gtttctgtgt 4980gtgtgtgctc agcccaaagg aagcattgga ctggctctgg gagtgggacg cagcgtgaaa 5040agatgtagta tctcctcctc tgcctgcttg tgttggataa gtggatcatt ttattgtcag 5100gttggctgag actgagcatc aacattatac aggaaagtcc caaaggtatg cagcagaggg 5160gatgctatac cagtctcctt cacttgatgc tattaaatga gctcacagtc tgtaagataa 5220aagttagata cattgaagga aaataagtac ctagttttat agcagtactt tatgttcaaa 5280ctcctcatga gtttatacat gtttctatag agagactgca tctaaaacaa cgctcttaac 5340aaatgaaatt tacaagaaaa aacaagctct gtgaattgct atactgatga gtgttttttt 5400ctttcaataa cagtagtaca atagtcactt acaatgtctg aacattctaa gccctaacta 5460tagatattat atttgattta aaatttcata aatatgttct atgctcatct agaaattttt 5520tcctgtgtta cacataaaga acattcatcc attcattcca caaaaacata tttgttacct 5580tctatatgcc aggaaaccat gatttctgcc tttgtaaatc tgtctagcat atgagaaaga 5640gtttttgctc taaaaatctg tgattatcat gtgttaaatg ggcaccacac ttcaaccagg 5700aataataatc atttttatat aatgatacaa tttccagagt gcacacacgc atacatatat 5760gtatggaaag agagagagat aatggtaata ttcaatatat ttaccaactg ctacagtaca 5820ggttccaaca aacctaaatg caaatggaat aagattgatt ttggaaaaga tctatagaat 5880acagaatctc agccattatg tccagttgca actatggttt agcaagctcc cactgatcca 5940aagacaggac cttaaactgg gaccttttcc ccatcttctc actataatat gagaacttaa 6000aagaggttat aatattggaa attagaaatt gatcattaat acatatttat tactttcata 6060ctatatgtca aaaactttgc ctggtgttca atcgagacaa gcctagcttc aggttaccaa 6120ggaataaata taaaataaac aataagcagg gggtacaatg agagaatact gattgtggta 6180gaagcatggc tgaggaggaa gttgtagaaa gagaatggaa aagcaagtaa aaaaggagat 6240cattattaaa gcaagaactt gcaagaggga gaaggtttga ggtcaaaggc aagaggaaag 6300gggtaaaaaa ggaagaatga ctatgttgaa ggaggaaaga aaataaggat ggataagaac 6360tgtaaatcaa tttagagttg caggagaagt agaataagtc cagaatgata ggaatctgga 6420aggaaagata gaggatcatc cagaatgaaa agaatagatg aactccagga agaagttata 6480aaggaaaatt ttcctgactg taacaactat ttcctctgtg aagtagggaa ggtaatctgc 6540aaagaataaa gagtgcagca ttaagtacaa agtataaaaa gctgtaaaaa ttttaaaaag 6600aaagcgtcat aggaaatagg taagtatctg taaagatttc ctagaaaaag attaagagtc 6660caacagatgt cagaaatcat aaatgtgtaa ggcagacaga caatcaatat ggttatgtga 6720tttgctccag gattattcag tgcctggttt caggatcaga acaaacagat ggttgggtta 6780ttctagaatc gaggattagg aataatggaa tgagaagaag gtcgtgagag tgttgggaaa 6840taagagcgtc agtgagagag aggtttaact gaatgaccat ggattgtcag ctggagtgaa 6900taaggaaatg aagccagaga agtcttattg atcaagaaca tacgaaactg acaaagtaca 6960gaagaatcaa tgagagtgaa gagcagtcat tcccctgaat gaaacccctc tgggtattct 7020gcagcctgga tgataaagtg ccattacttc ttaaaacata caagactgtt tataatcttg 7080cctgcctctc aagctcattt ctaattcctt ttcccaaatg cctccaggca ttctgtatgt 7140cctgtcaggc agaccaaata tgatgctttc tgtcttcatg ttactgctgt tctttcaaag 7200ggctcctctt cttctcctaa acttcctgtc ctctattcac taatgaactt ctcagttttc 7260aagacccact tcaagcatct ttcttattaa agtcacgtgt agtacttagt atgaaaaaaa 7320atggccgcca aaatttcctt ccacccttat acagtcatgc cattactccc accaaaaaac 7380cccttgattc tgggcaaaca aacagaatgc tgtagaactg acactattcc agttccagcc 7440taacccaact gccatattgc gaagaagttc aggctatctt gcctggagta tgagatacca 7500tgcagacaga aaatcataga gaggagagat tgcatatagc aactatacct cagacatatg 7560agtgagatct tcttggccat tcctgttcat cccaaccacc agatgaatgg aggtggagcc 7620cagtcaaccc acagaattat gggaaataag gaatcatttc cattttaagc cactatgttt 7680tagggtttag ttattcattt ctagataaat gatatctctc ccattttcaa cttaagtgta 7740actgttcaaa ctttccactg tatcatacta ctgcatctat aatatacctg tattatttta 7800cttatcacac taggattgca aaattgtttt tacatacctg ctcccttcct attctgtgag 7860atgaaacaat gtttcagtgc tagggtatgt ttacactatg tgtgcgcata tatgtgtctg 7920ttgtgtgagt ctgtattcta agtcctagca cacaacatac aataagtatt taataattga 7980cttgaactac attaaatatg gattgagtta gaggaaggta gaaagagaga gctatgaggt 8040tcagtcataa gaattttatg gcttatttca taaaaggagc agttccctga ttcacacgga 8100aacatgttgg aagtgaagtt ggcttaagga tagtctagga attcaggtgg ggtggtagaa 8160tcatcaagga ggtggacttg tggcctacta caattacaaa atttgtgtgg aatggaatat 8220ggtgatatcc aatgggaaga gattgtgaac ttcagaaaaa gagaagctgt gaatgacagc 8280tttccagtga agaaaaggct cttaaagtaa tagtaaacca ttaagaatat gcagtttcaa 8340ccttctcccc aatgcatgtt caagggagaa aaagtcagtc tccatttcac agacttacgt 8400ggaagttgta ctttaactaa aggaagtttt cctttaagtc atctgagtgc accttttcag 8460tctcctccag cgaccccttt cgttccactg ccactgaaag ctttctacac agtcctctgc 8520cattctcact ctacatctct tctctgggcc atgtgagcca ctcccacggc ttcgatcatt 8580actggtatct cctactgctg aactttctga ctccatttct tttatgagcg tcagaactat 8640tatttacaat tctcttctga atgtctccag tgttttgcct accacttatg taaaactcaa 8700gatgttaaga aacgaactga ttatctgata ttcaaaacat gtttttttcc tcccatattt 8760cctagtccag taaatggcat actggccact ccatgagtat agaaagaaac agccagcaca 8820ctggttaaga gcttggattc tggaattaat gaaacagaat gtgctcaaat ctggccttca 8880taaattacta actgtatggt aattctagac tgttcaggct actgtaacaa aataccagaa 8940gttgggtcac ttaaacaaca gacattgatt tctctcagtt ctggaggcta agaagtccaa 9000gatcagagtt ccaccacatt cagtttctgg tgagggcctt ctttgtggct tgcagaagct 9060gccttcttgc tgtgttctca cacagagcta cttctctggt ctcttcttat aagggtacta 9120gtgccatcat gagtaccttt atgaacttat ctaaacctat ttacctctta aaagccccat 9180ctccaaatgt cattacattg agagttaggg tttcaacata tgaattttgg gggcacacaa 9240ttcagtccaa agcagtaacc ttggcaaatt acttaacttt tcagagattc atttttctca 9300cctgttagaa tcttcaaaag cttgtgaaga ggattaaata aatgcttatc caaaccctca 9360aaactaatca taccaactag atctcttgaa gccattctta ccttctccct ttaccttagc 9420acgcacctct tccaagcaac tgatcgccaa attgttactt ccatttctaa agtgtacctt 9480aatacgtgcg tttgtccatt gtcacttcca ctgtgctagt atagacctga acatatttca 9540atcagactat tcttgacttt gtctaaccca tctttcacac cattgccaga gataatgttt 9600ttaaaaatat aaatctgatc atatcctttt tcttcatttt caattttttt tagtagctcc 9660ctgtttccta actctctagc caaactctct agcatctgat ttttgcagtc tttctctgaa 9720ctacgtttcc agggccactc tacatcattt cccaccattc cttgtattta aaaatgtcat 9780tctgtgaaca tattcttctg ccttcatgtc tatttccttt actaaatcct cacccctact 9840tttccccgag aattcctgct caaactccaa gacccagctc gaatgtgatt tcctttctgt 9900tatcttccca aaacccccag gtgacatagg ttgcaccctc atctgttttc ccacagggct 9960gtatacaaac ctgtctaggc tttatcacac tatagtatag ttattaattt acttgtctat 10020cttcccagct agactctgag cacttcctca gaggcaagga tagtgaacta tttattcttg 10080cattaaaagt ccataataca gttcctcgga aatactagtc agtatatgta agatgcttga 10140taaatgttgg ttgaaagatt gaataaataa tctatgtaac tttaatggtg cctgctgtat 10200attaattaag ttcaaatttt tcaggtataa gtccaatttt tcaggtatat ataaaaagta 10260aatcattaaa actgggcatt gaaagaagta gattatctac acgtattttt cccagcttta 10320aaattctata atcttatcgt gaactgagct aaaactcttc tccaggcata taggattaaa 10380gtcttgtctg agacagacta aatttgtaga taggatctag tgggttgttt aaggtatatt 10440cccaatttat tcattacatt aacttttata aactgtattt tgtgaatttt atacctttca 10500attaaacaaa attaaattat atgcaatagc tgatctttca aaatggtaac atggtgaaga 10560aagtgttttt ttctgaaacc ttgtcagctt gagtgacatg tgagtaccca gcactaagca 10620tctgttaggg aaatcttatt actctgtgtc tgtcactaga aaatgggaac aaacgtcgca 10680agacctacat acttaaaatg ttatgatgtg tttttaaaat tgctttccac ataaggtctt 10740ttattttatt agaaaattat aagcaaacaa ggcttgttta attgctttca aagtataagc 10800acatttcaca tcaaaattgt atcactttca actgctgact tgtgtgagaa agctgtcagg 10860ccctggtgcc caagaaaata gtgcaccatg gcagaaattt cttactgaag gatataatgt 10920atgctcaatt aaaccaatgc aatgtgtgta atggtttact tctatcttag aacgtactgc 10980tagctttctg ttctggaatc agaattttcc taatgcctgg actttttgtt gaatcccatg 11040taagaagtga tgcagtcttt gagtttatat ctcagggaag gttgggctct ttttttctcc 11100ttttaacatt caaaaattct cagatatatt accttctcat tctgataatc tatatatact 11160ctgctccctt tccataattc tcttagctta ctaaatatct tagctaacaa ttaaagaaat 11220tatgcatgaa atgctaaaaa ttatagccag cttttcagag gaatcaattg ttggttatag 11280tgctattttt cttaaaaaac attgccaaat cctttatttc ttcaaatatc atagatatct 11340atatgtgctt caaatgaatg ggaggtaata aaatatgatg gaaaaatcct ggaaaattcc 11400aacacacctt atattgtttg ttatttgaca agtttataac aactttgctt ctcttagcca 11460gcagagagca cccatagagg tggtagatga taacttttcc acaaaatatc gcaataaaca 11520gataacattt aagatatata ttttcatata tattctaaga aaagatgact gctaaacatg 11580aattccccat gatagaagga tattgtatgt tactaaactg ccatctactg gatttgttca 11640cccagtacac aatatcatct ataaatttgt atttttcaag gaatacatga ctttttttcc 11700cagccatttc ctgactttct ttctttccct aattctagca atgtgtgctt ctaatgacca 11760ggagaaaaaa ttgcttcaac taattatagt ccaaccaaag tcattcacat catttaagaa 11820ttagattgat tattcatagt gtcatgagga cgtgtcaatt tgagacgagg acgtgtcaat 11880ttgagacaag aagaggttta aagaggctta cacttttcag agtgccacct gtctccaaag 11940ggcacattca ttaagaagct ccaggtgcaa gttgtggaag caaaacagca taatgaagtc 12000ctttccctcc ttgtgcacct tttgattaat aacatcctcc ttcagttcta gcagattgca 12060caccagtgta tgccacaaga aacctaaaat gcaagcccac ttttctatcc ttctttgaat 12120agagtaaatc accatttatc tatgaccaga atcaaaagaa ttaacaaaat aaggtatctg 12180tggcacaatc tacttttata agaaaagaac agctaatacg ccgactgata atatttactt 12240gataacaatg tataatatgt gtcactgata aaattccacc taatctatct tctctgtcct 12300atattcacac atggaaatgc aattgatatt tggctgggtg cagtggctca cacctgtaat 12360cccagcactt tgggagacca aggcgggcag attacctgag gtcaggagtt cgagaccagc 12420ctggctaaca tggtgaaacc atttctacta aaaatacaaa aaattagacg ggcgtggtgg 12480tgcgcacctg taatcccaac tactcagaag gctgaggcag gagaatcact tgaacctgga 12540aggcagagat tgcagcgagc cgagatcacg ccattgcact ccagcttggt caaaaagagc 12600gaaactctgt ctcaaaaaat aaaaataata ataataaaat aaaaaatgga gaaaattggc 12660atttattgag tactatttac tagacttttt tttttttttt ttttgagaca gagtcttact 12720ctgtcaccca ggctggaggg cagtgacaag atctcgactc actgcaacct ccgtctccca 12780ggttcaagcg attctcctgc ttcagctgcc catatttact agacattttc atgggcgcta 12840tcacacttta tctacacagc agcctggtaa ggtaaatacc gttgtcctca ttgtcatcac 12900tgaatgtgac tgaagctcaa agtgataata agagctaaca cttattgact acttaatatt 12960ttcctggcat tgcctttatt actttagaaa aattgacaca attaattctc aagaccacat 13020tatggaatac atgttaccac tgtcctcatt ttattagagg tgactccaag aagctaagta 13080acctgcccaa cattacacaa ataatatgca atggagtctg gatttgaccc gagcaaactg 13140gtttcaatgc ctgtacctgt caacttcaac attatgctgt ttatgagtgt gtgtgtgtgt 13200gtgtgtgtgt gtgtgtgtgt gtatacttca gtattagatg ggctatatta ttgttttgtt 13260tctgaaaatt ggtaattgtg tatttattgt caggcatttt acatgtatta tccattagaa 13320atacacacat atatgtaata ttttccccat cttactttga taaaactgaa ttttggagaa 13380taacaataat aagaataatc acagtaaaca cattacacat atcacatgct agatactctt 13440catgagaaga gttttaaaca tagtacatat ttaatactga caacaacaat ataatgcact 13500gttatcacta ttatacaggt gaggaaaaag aaaggtagca ccttgaccaa agtcgcaagg 13560ctagcagaaa gcagtgcaag aaatctagtt ccagactcat gattttatcc actactctag 13620actttgaaac aatttagaat agtcattcct tgtttattgc agcaaatgtt tgcaacacgc 13680cttaactata atagcattca tcacaatcaa acattttgtt gcataccaag taaaataatt 13740atgcacattt ggtaaagagt aagaaggaat caagcctgta cagtttcaca ttggtcaggc 13800ttatgcatag tttcaataat ggtaaaaata aaattcctct cttcatttat cctacaaaca 13860tcgagcatta accttgttcc aggtgcttgg gtagtgagaa taatgtttat tttattctgc 13920gaagaattgg agatacataa aaacaaatag gtaattgcaa cataaaagga taattgctaa 13980gaagaagttt tgcttcctaa atcaaagcca aagctacaaa acactttatg ttacctggtt 14040tcatggctcc aaacgaggtc atacacaggg caaaactagg aaaagaataa aaagacagaa 14100gaacattctt tcataaatac ataaaataag gtgcttaaaa ttgttataat agttgcaatt 14160ttattttaaa ttctgttaac gtttatgtct gaatcacaag tggaagttca aaatagcttt 14220atgatatatg tattccttgt ttcacatctg atatttttct ttgagtacta ctccttccta 14280gtgggtttgc ctggtcctga gttcccaggt cctcccagtc tggctcatgc caaattcaag 14340ctatcatatt aatcagaatt tttagttgca ggtgacagaa ccccagtgca atgtagctta 14400agccaaaaga aagtgtgctg acatgactta ggtgactggg aagtccaaga gataagcatg 14460cctcagatag agctatattc agggatacaa atgctatatt cagagttcac ttttttccct 14520cgtcccactt ctttctcttt aattctctcc atctatcagc tcttattgta tctacttgac 14580ttccttttgt gggtaaactt tctctatata ggtgggtctc acattcacat ataccagatc 14640agcaactcta atgaagaaaa aacttctctt tcccaataat cctaaatgaa tcctcgggaa 14700tattctgatt ggccttgcct ggttcatata ttcagttttc agacaatcac catgattagg 14760tgagctaaca cacagaattt gatgctcgaa aaggactcac aaaaatcaga aatagcatca 14820agctttccta ataatttcag gagtacaggc aatcaagagg caaagacaga gcagagaaag 14880tctgggacat tctgtgtggc tttgccagat gcttcctatc cactgacccc cagtgattga 14940ctaggtctct gtacgtagtt ttgctgatta ttaccttaca gagtagaggc gtgtaattta 15000taatacatct ccattttata cctcagaatg ttataaaatg gcatgccatt acaagacatt 15060cttcagagaa gccatccatg tccccataaa tcaatagatt ccagatttat ttactagaag 15120taatccttat ccagggagat cctgctaagg gctttctgta gaaagatctc cttctgtcta 15180aaatcattat tctatttatt agggtgtcta gtcagaaagg agattagaat agattacctg 15240ccttcctttc tttttcctag tggtgccccc ataggaaagg aagttaatgg tccacagggc 15300tagctgcttt actccttccc cccatcagga gactggacat tctgactgaa catcttgaga 15360caagaatctc aaactgtgct cgggggtaaa ggaagaacca tgattgacag gcccccagaa 15420gcacctggac tgtgagatgg cgatagaaaa gcacgttgag tagatataaa ctgcgagtgc 15480tcttgtttat catactgttc ccgtccagac aagagacttt taagagttct aatgatgcta 15540taactttcct gttcctaaac tttgtctatt tctctctggt ttctaactgt gaaaaactct 15600tcagctatgt tctgtgactc acagatcaca gcaaaagaac aaatagtgac agcaaagaat 15660ggaaacaact atgccaaatc ttggagcccg caggaaagaa atgcaaaaga atgctgagta 15720gggcctcaca atgaaccctt aagaggagaa agaatatccc aagggttttc catctaggct 15780tatgtcctca taaccatttt cttacagaaa tctaaagttc taacatagca aatgaatttc 15840tttaacaggc agggtacaca aacaactatc ctactgacct gataatccaa aagaactttt 15900tatttggcca agacaacatt tatgtttagg ttgaatttga aaggctttga acagctcatc 15960acattttccc tcattcccta gtgttttaca caggcctgca tcattgattt atgttactgc 16020ctgaaccctg taaacatttg agtttttctt tcatggttta atacttgatt taactgaaaa 16080tccctttata agaatgtaaa ctttaggcag ggtgcggtgg ctcacgcctg taatcctagc 16140actttgggag gccaagatga gtgcatcgct tgagatcagg agttccagac aagcttggcc 16200aacgtggtga aaccccatct ctactaaaaa tacaaaaatt agctgggagt agcggtgcat 16260gcctataacc ccagttactc gggaggctga agcagaagaa tcgcttgacc ccaggaggtg 16320gagaggtgga ggttgcagtg agccgagatc gtgccattgc actccagcca gactccgtct 16380caaaaaaaaa aaaaaaaaag gacactttaa actgttttta gttatttaca ctaggatgta 16440aacagttttt caactgtttt tcactgtggg ggatgagagt ttttgaagaa ggtaagactg 16500aaggaataac aggatgggga ctagggtgag ccagatgcct agggttcaac atttaaggag 16560gcgctcattc tcaagtgata actccaggct cagcacctga gactgcctcc ttaaatgtca 16620tgccctgttt gtgttatttg cctcgctctt atcccagtcc tttaaggata aagtttaacc 16680agctttggtt tctgttactt gtgatgaagt atccttaatg catttccctt ccccacccat 16740gaactgaggg agtcattctt cctgggaaca cccataatat cctctgatta tacctatact 16800atacttacaa cacagttttc tcactattta cacatccata tgcatcggca ctctgtgcgc 16860tccttaaaca aagagactgt gacataatct tctttatagc tgcaatatct agtcctgtac 16920ctgttgagtg ggtaagtcaa tgaatgaatg gacaaataaa taaatgagtg tgttgtcaga 16980attgaacatg ccatacctgc tgtggcatgg cacaggtgtg tgaaatcctt cgtcagtaaa 17040aacattttat ttttgctata gatagatggc aaacttcaac ctgcttttat tctaattgtg 17100atatgatttt tctcataact ttgtatgtag catttgaatt gttccagttt tttatttaaa 17160catacgtgct caagaaagaa ctgcagtaga tttgtgactg acaccgtata tatgcttttg 17220ccctcagtct ccagcagggc cagcctcctt gacctaatta gacttctcac tcccacatgg 17280ctggtaaata ctataattaa tttataaagg actctgaaac accaactggc aaaaattccc 17340tattaatggt ttctacagaa tgaaaagagc atttaagttg taggagagtt gttgttgtgt 17400gtgtgtgtgt gtgtgtgtgc gcacatgtat tcacattttt taatgtagtg aatttctaag 17460aatgtcaaat cataacacgt actaagtaaa caatgtttaa taacgtcttg tcatttcttt 17520cagcacattt attttaaatt tgaaagaaat ctgtgtgaca aaatattctt agagatgtat 17580atttggggta tgtgagactt gagtgtccat gaatactcac acacatccca taaactatgc 17640cattcttaaa ataaagttaa agagaaaaat atagaccatc agtttaagaa ggatgtctca 17700atgatttgag aaagcaacaa ctgtagtggc ctttggagta tttgcctatt cagaagccat 17760ggttggcaga atttcaagat ggctcccaag atttctgtgt cctggtgtac atgccctgta 17820ttataccctc tctgtgagtg tgggcaggac ctgtgaatac aatgagatgg cactcctttg 17880attagtttat gttatatggc aaaggggacg gaaccatttc tcctctgatt acattatatt 17940atataagact ttatagcagt aatagttata gtagtagtat atagtagtaa tggtagtggc 18000agcagcagca gccaactgga gagaaattct cctgctggct ttggagaagt aaactccgtt 18060ttgagatggc catgtaatta ggacctgagg gcagtctcta gaagctgaga gaaccccctg 18120gccaacatct cccaagaaaa tagggacctc tgatgtaaag tttcagggaa ctaaattcca 18180ctaacaacct gaatatgcct aaaagcttca gatgaaattg cagctccact tggcactttg 18240attgctgctt agtgaaaccc taagcagata atccaattaa gctatgccca gacttctcat 18300ccacagaaac tgagagatat caaatacatg ttactttaag cttctaaggt tgtggtaatt 18360tgttactggg cactagaaaa ccaatacaag acccaacggc tttttctttt ctgtgggcac 18420taccctttac tccccttgtc ccacaggagt gcctcctagc agtcaggtct atacattaca 18480ccatgatcct gaaaaactga catagctgat tggatagagg tgcacacctg actcaagcca 18540gagcaactag agtctggaaa tttgaagaca gggctaagag gctccaggtg ggaatttctt 18600gaatggaaga gagatagaaa cttgaacact ttagcaacac tatcttctgt ccagggttgc 18660ctgtagatat taaaagccct gaggaaatgt tcgaggagag agagaatcta tctatagcaa 18720tcatctgatt acaaaaataa ttataaaagt catggaccca tgtgagatgg agtgatttgc 18780tgttaaagag tcaaggtaag gaaaagctga agagagtcaa ggtaaggaaa catagctact 18840tattgtccaa tcggtgaaca taaaaatttt ttctagtttc ttggagtcac ctatttgtat 18900taattattac taaagaaacc attcctgttc aatttcatat ctaccactgc aaggaaaagg 18960tagtaacact attatcattc acaatgcaat ggctcttcag gtcctattat aattggaacc 19020gtatagatct ttttgcctct gcagttccat aatgccattt atttaatgct gattacagca 19080ttactcatat tgcttcaaca tccattgtac tgcctgtgaa cactggatac taatgactca 19140aatggtgtta gtgtatttgt tcatgatgac cacaaacaca ggtctcacac agagaatgca 19200ggaaaatgca aacaattatt catactgtgg tcatgttaaa tttattgttt ggaatgtatt 19260aatagcattt atttatagaa cttactgcat taacatagaa tggcatatct ctcaaccatg 19320tcctctcttg aattgtctag accttaatca ctgcatccct agaatgtgac tcttttaagg 19380ttggctgcag ccctgctttc catatatagt tatcagtggt gaagatagat gagaggccct 19440ggatagatga aaagagccat cccatttggg caaccagtgt ccatcacttg ttcatcaagg 19500cttacaacca acccagaaga gaatgtcacc atgtttatta gaggtaaaaa agacattgat 19560tgaaatggcc cattggcttt gtgttgctac agtctcaaaa gccctgaaag gaggcaagca 19620ttgccatgat aagtagtgtc tgatatgtga cacggagctt gcagaggggt ggtgtgactg 19680ccctaatgcc agcagcaggg tgacggcagg agaccccagg ataaaggatg tctgcagaca 19740cacatacctt tttcaagtgc tctccactag aggtggttcc ttggtctgac cagtcaggga 19800agagcccaca tactggctca cacagtcagc tccagaccaa ggatgcatgg ggggacagtg 19860gtagcccaga gcactgaact catcctgtgt ctgatattag tgaaaatcac cctcaaacca 19920ctgaaaaagc atcattctgg tggcccaaac ttgtgcgatg aaagaaataa tgccatgaaa 19980gaaataatgc cccagccagt gggagctttg gccctccaag ttgctctcca ggaactggca 20040gggccatctg gccctaagcc accctacagg tgctagtacc agagatcctg ctgcactggt 20100ggaccctgag catgttaaga gggcaatatg gtcaggaggg cctgaaggga agaaacaaag 20160aagccaataa cattttcttt aggcttagga ctgtcccacc caacaggtcc tgctgacctg 20220gctggtcccc accaccaaaa gcaatagaac atctcaggac ttccactgtc caggacccac 20280atagtactac attttggctc catcctgctg aatggtccaa gacactcgag gggaattagg 20340gcacctcaag gaaggccagg tgcaggacca ggctaggagc cctggttgcc caggtctaag 20400ggcagtattg cttttttttt ttttttcagt cttctgaact aggtaacaga aaagctagtc 20460tgaggaaaga ataacgcata tgcagaacaa attcagcaaa gagacagaga aagggtgctg 20520cttgaataga ggatagtttc ttttaggata tttctgaaac tgcattcctg ctctggcatt 20580ccatcaaaca ccaccttctc tttataatat gctccccctt tttgttgttt aagtcatttt 20640atttttattt ttgttacttg caagcagagt gagccctaat gcaatattta gagccagatt 20700cttagctttg taggttcaga aactcaactt taccatttcc accagagcta atctcctggg 20760gaccaacata ttaagaggac ccataaacaa ctgatatgca tttcagagtg ccaatttctc 20820aatctataaa gagagcttaa tccagatgaa caacttgtgt ggataaattc actctgggaa 20880tttcaccaca gtaggttaag tgtgctcagg agagaagaca aattatcaga gggctgtttg 20940gtgagcatat aataagaata aagagaatgg gtagaatttt tctttcaaat taggatagct 21000gaaatgaatt tcttttgtag gaatatttct ctttgaaaaa tcagctaaag tccttgaact 21060caaataacct cttctggtca ttatcagatg tattcacgca ttttgttcat tatcctagac 21120ttactataga ggatttttct cttgacacat aattctccca gaaaaatttt gctaattaca 21180gttagtacag ttgattaact gttgttgcat taactgcatg tcatctacca gctttggcat 21240ctattattcc tattgacccc atgaagtcac tatctcctct ctactcccaa gggtgatcac 21300tatccaagat tagtttggct gcttttgaac ttcatgtaaa tggagtcata tagcatatgc 21360cattttgtgt cttgcttttt tcaattcaag actgtatttg tgagtgaaat actgttcaat 21420aatctgagtg taatttgtta ttcttgctac tgtattacac attccaactg tgtaaacata 21480tgacaattta tttatctagg ctaccactga taagcaattg ggaagcttcc agtttgggct 21540attttgaata gtacagctct gaacattact atatacatct tcagtgaaca ttatgtgcat 21600tcctgttggt aatataccta aaaatggaat ttcagggtca tagtgtacga aaatgttcag 21660atttaatata ttccgacatt ttccaaagta gatgtatcaa ttactttttg caatgtatga 21720gaattacagt tgctctacat gctttccaac acttagtatt ctctgatttt tcattttagt 21780cagtttgatg aatgcataat ggtatcatat ggttttaatt tgcattcccc tgacttgcag 21840taccaaccaa gacatagaaa gcccacaata acctatcttt cctaatgatt acagtgagaa 21900actcaagaca aaataccaaa agcaactgcc tgaagactct ggaaaaacaa aaacaaaaac 21960aggtaaattg gagtggagaa tgaaaacctg gagaaatgtt ctgtatgttt gcttttcctc 22020ttttctcaca gcttttgacc taaggcccaa cacctagtga aactgtggtg acaggaaaaa 22080ccgacagaaa cctgtatgtc ttgccagaat ggggataggg ggcttttgtg agccaaaagt 22140tagtcggggg aatacttagc tccccctctt ctggtttttc tttctttttt ttaatttctc 22200tctctctctc tctctctctc tctctctctg ggcattggcc caagagtgag gccctaggct 22260tgggtatcta caatactaac agaagctctg tttttatgga caaaggaagt agaaaaggag 22320gcctttgtgg tctgaagagt gtagagggaa tccacatttt ttctctctct tttctgtcac 22380tgctttgtct cagaatctgc cctagtcagc tgctatgcca ggctgctaaa actccaagag 22440gaacctcata tttctggtga gaggaccttg cgagacaaaa ggagaagaat gtaagaggag 22500agagctagag aagaggatcc cctaattcta tgtatgaacc tatacaagtt ctgtgttcac 22560acctaaactg aacacgtgca gaacaaacac aaaatagcat agcaaaactg aatttcaatt 22620taaactactg ctaacatccc caaataatcc ctgagtattg taggtatagg acacagcaaa 22680gcctttgaaa acccagctga catggaacca gtactcatag aagatgagac cgaacttttg 22740acctgaacca aattggatcg attgccttct aaaacaaaaa aaaatcaaca ttccctaaag 22800gcttttaaca ggactcagtc tatgcaacat aatgttcaaa atgtcctaga taaaatccac 22860aattacacag tataaaagaa ctggaaaaat gtgaccaact tgcaagggaa aatataatca 22920acacctgcta actcctagat gaccaaaatg ttataataca gattttaaag caacaatcat 22980aactagagag tagttgaaca atttctctcc taaacataat catacttgca taaagacatt 23040tcagtcaatg atgaaccaca tatacaaagg tggtcccata agattacaat accatatttt 23100tactgtacct ttatatttag atacacaaat accactgcgt tacaactgcc tacagtattt 23160agtacaataa gatgtactaa atgcaggttt gtagtctagg agcaataggc tataccatat 23220agcctaggtg tgtagtaggc tatacctgct aggcctgtgt aagcacattc tatgatgttc 23280acacaacagc acaattgccg aaagtggcat ttctcagaac atatccctat cattaagtga 23340ggcatgactg tatatgctgt attctaaaaa aaattaatgc atttatatac cagaatctat 23400gcataagaat gctcatactg gtgttgtttg taataccaag cctgagaaac agaaggaata 23460aactgtcaca aaaccacaaa acggaatagt atcagtatac atcagtgcaa ataaaagaat 23520tacacctata cacttctaca tgcaactacc taggattaat ttagaagcat aatgaacaaa 23580aaaagagtac agaatcaact atgcttacac agctcataaa attaaacata catgtatgtt 23640aggttttgaa gggaaggcaa ggattaaaga aagagagggt ggcttaacaa caaatacagg 23700atttatgtcc agcataagac ctacagaggt gggagaccag cttaatgcca gtgcccgttg 23760cttcttacag gctggggtga tttataggcc tgggtgggag tggtctgggg ggccatggcc 23820tgcttcctgg gaacatgttg ataaggtgtt cccaggacga ggcggctctg gcccttgttc 23880cagtggaatg tggtactttc tcccagcaga gtataagagg cagcctgttt ctcacgcccg 23940aaccccgtgg aatgtttcac tttgactaag gtctgcaaaa tggcaggggg gcttacaaaa 24000atggtgcagt ttggactaac aacgtacata aatttttaaa aagcaaaaga ataaatccca 24060aatatgggaa agtgttatct gtggaagaaa gtaatgggat ggtatcagaa aagacactgg 24120taatttaaac agtgttgaca atgtctcagt tttaaaatta ccagctggtt cccatgtata 24180agttgcttac ttatctgtta cacctattta tgtatatata aaaataggtt tccaaacgac 24240atcccatgtg tatgaaataa cagaattttt atgccttatt aaaataattt ctctaaacac 24300ttgaagagcc cgagccgcct cttaccctaa acgaacccca cgcccctgaa ttgatcagtc 24360acttccgcct ccacctttcc agctccacct aaggaagcgg aagtacttta ctcttgggtc 24420ctcccacctg tagggggctc tccgaggctt ctagtgccac gaaggcctcc tgcctgggtt 24480gtccttatgg cgcctgtgag tttagttcta gaaaagatat ttgtatttta acttttccat 24540ctaacaaaca gtagcacagg catcttagtc tggacaacaa cgtacaagct gaattcctgg 24600ctagttccct cattatcaaa ccctgacaca gtagtaggcg cgagtcaggc agtgtgggga 24660cagccccgag ttgccgaaag tccccggccc gttttcctcc tgtgaagaca tagctgcggg 24720tggctgtgct ggtggcgttc aagatgtcga ccaagaattt ccgagtcagt gacggggact 24780ggatttgccc tgacaaaaag tgagttcaag aagggtcctg aattgggacg gagtttgtta 24840gtggaagctt tccgaggtcc gtccggcatt ttcctttttt atgattttct gtgccttaag 24900acgggttgag gcggcgaacc gccagttccc tggcgggaag aaggctccca atctcctcac 24960gagagaggtc tttccgggag cctttgttct agatatgtct tcagcgtttc ctcgacatta 25020cctccgcaag gtagcctctc tttttagagt gcgagatggc ctaccttttt gcccccaccc 25080ccctcccccg ccccggccag gttttagcga taggaaacct ctccagtcag gccagcttgg 25140tgttggatct cccccacgtg acttttctct gcagaacttt actcttgttc acggaaaata 25200ttgctttctc gcactcgtcc ttttgcctct tatagttcac cttgttttgt atttttactc 25260aaacttttcc tccatactat tttccccttt tccggaagct ctaccctctt cggtttggga 25320aattctgaca ttttctttgt ggatcatttc tccacaagaa ttgggctttt cgcctcctta 25380gagccgaaaa ggagggaaga ggcctgtgtt tttgcctact cagcccttaa gatgcctgca 25440gcagagaagc acgtgtgtta gtggctctgc tttttgacat tgttgccaaa gccagtactc 25500gactagatag ttaatcagat tttttttgaa gggtgcacaa ataggtgtat ctattcggaa 25560ataacatgga aaataattta atgtacaact catgttcaca taaaagcctt ttatttgttg 25620tacctaagtt agccacaaat aggttttgat tgtaaaaagc aaaaactttg aaataaacct 25680gggtttagtt aaaattctat tacttattta actgctaaaa aaggaataga attttttttt 25740taatgaaaac atgaaataac atggataggg tgtgtaaaca gtgacgtgta gcaaattcta 25800attggtggct attgatagta atttgatatt aagatgacct gaaggagtag ttcaagccta 25860acaaatgata ctgaatagaa ccaaagttat atgttatgga tagtattgct ctgagtggta 25920agacattatt gaaaacgttc aaaagattta gaacaacaac ttctgacttt gttgttacct 25980gtaaaataat gtgcttttgt cagtgtcttt aatgacagca cacccagagg taggactctc 26040aatgagagtt catttcaact catttacaaa tatatgaata gaatgttgtt tgtacaatat 26100gtttaaatgc aggataaatt ttcagagtat attttccatg cttaatggag ttaaaaggaa 26160tatttttagg ggaaaaactt aacctaaata ttatttagag tgtttcactt tagtgtcatc 26220ccatacttca catctgtatt gtattctagt ataatgcctt aaaaatcagc tttttaaagt 26280ttaagcattc catgttatcc agagcattta acatactttt accaatatac acgtacactt 26340ctaaggtcat gtgagtgaac cagtaattct ttaggtttga gtttgttgag tataagcata 26400aagtcttact gtactacatg tgttgagatt attggttttg gtggttgtgt catttttttc 26460agcgaagtct cagaggtttg agaaatgccc tgtaaaactc agttttctta caattattga 26520atctttttgt atttttagtc agtacaacct atcatatttg gttccttagc atcctaccta 26580ctgcatgtaa aaagataatt tcattaagat ccatgaagcc ctccttgatt tataagtttt 26640tttcctcatt ggtccaccgc tagggtttaa aactccctgg tagcttgtaa gtaattagtg 26700aagtggagtt aactctcact ttcaactttg taaagggatt ataactaatg atatgtgcta 26760atgtgaggac ttgaggcaga gatcctaagt aacttaatca agatcacatg actggtaaat 26820ggaggattgg gatatcttat tctgaatttg tggctccaat caagtatttt tcccccagta 26880aattgtgtgg agttttttgt tggtaacatt gacattaaca tgactaagat ggattagaag 26940ttatgaattc cagatatgta gtgaggacaa gttaaaatgt aaaattttac aaatttaaat 27000ttttataaat gctttttaat ctgtttttag atgtggaaat gtaaactttg ctagaagaac 27060cagctgtaat cgatgtggtc ggggtaagta tttaaagaag attctattta tactgtatat 27120gtatcattta tttatttctc caggttcata ttgcatgatt tttctgtttt cagagaaaac 27180aactgaggcc aagatgatga aagctggggg cactgaaata ggaaagacac ttgcagaaaa 27240gagccgaggc ctatttagtg ctaatgactg gcaatgtaaa acgtatgttt tttaaattat 27300tgtctgctct ttcttccaaa ataatagatc tggccactac ttgtttatat tacagctctt 27360attagtgaca ttgacaagcc agggtaacct tttaaatcca aggtattgtt attcagtctc 27420atctttaagt atagtataaa gaagttttaa attattcttt gcatttttta atggtaatag 27480ttgtcactat gatggattat gtataccagt aaacaaatgc ttatggtaag ctcaaaacca 27540atcggggggt ggtggtactt aaaagtacta aggcctatta cttcctttat tgaaaaatcc 27600caatttttag actgtatagt tagtgtgaaa tacaccacaa aactcatttt gcctgtttac 27660aaaataatgt tagattcata agttgtgtgc cgtaagtaat taagtctaat tataggtagg 27720tttcttaatg atttttattt tcaggcttag atttaattct tcattgattt ttccaacttc 27780catcttttct ctatgcagat gaatcctatg tgtatgaaag aggtctaaat caaaaaagta 27840ttaaataaat ttctcttttt gtttgtttgt attttttgtt tttgagatga ggtcccactc 27900agtcacccag gctggagtgc agtggggcga tctcagttca ctgtaacctc tacctcccag 27960gctcaagcaa tcctctcact tcagcctcct tagtaactgg gaccacaggc ccatgccacc 28020atgcctggct cattttttta tatttttggt agagacaggg ttttaccatg ttgcccagga 28080tggcctcgaa cacctgagct caagcattct gcctgccttg gcctcccaaa gtgctgggat 28140tacaggcgag gcatgggcca ctattcctga ccctagagtc ttaaataaat ttctgatcct 28200caggattata aatttgtatt ggaaaagttt ccagctacag atttggtcac tggatttggt 28260atgctttgga attcagtgct tgaactgaga cctcaaaaaa ataaattaat attatttata 28320gggtataacc atactcttta aggtatattc agagactgag aaattagtgg agctgttaat 28380ttaaagttat attttttcat aagacatcag aatttctata caggttcagt atcacttacc 28440tgaaatgttt gtgggttcag catccctaat ctgaaaatct gaaatgctcc tgtgagcgtt 28500tctgttgagt gtcatgtcct ctctcaaaaa gttttagatt ttggaacgtt tttggatttt 28560ggatttgtgg attagtgata ctcgacctgt ggttataatt tgtcatttac ttctcaaaat 28620gacagaggca cttttcatac tatataaatt ttgcttctct gcttaaatgg ttcagtccgt 28680tgttggaacc cacatttact cttcaggcca ctgtacattt tctttgttta cattaccact 28740taataaacat ttctttgaca gtggtttcat cctagttttt attttaaaat tgtaaaatat 28800ctaaaaattt gatattcatc tatattatag cctactaatt tagtattttt cacttctaaa 28860gttgcagcaa tgtgaattgg gccagaagat cagagtgtaa tatgtgtaat actccaaagt 28920atgctaaatt agaagaaaga acaggtatga taaaaccaca ttgtaactaa atgatttttt 28980ttaaagcact aaatattgaa acgataattg tatcaccttc gagtaagagc cacagtaagt 29040gaggattctg tttattctgc cctgaatgtt tgttactggg atttttttcc ccccatacac 29100acatacaaat cttttgtttc acaatttata aaataacagt gttagggacc tttatcctaa 29160caaggaggga cataagaata ctttcagaaa tattctcctc ctctcccttt cactggtttg 29220catgtttgtt attagtaatg atataatgat accattttta aattcttttg atagcctgct 29280gtgacttaaa gggcaaagcc acaccattta aagtgttctt ctaaagcttt ggcagtaatt 29340ctgaaactta agctatgtgc tagattatct gaaaaacttt ttcaaatatc gattgcaaaa 29400tgcagaactg ttaaagttat ttggcggtgg gtttccaaca tctgcaggtt ttttggatgg 29460tgtttgataa tcattggcct aaataatagt tgatcattgg ctttgtatgt tactggtgaa 29520atgaaaatgt ttacctaata gcatatagtt tttccattat caccaattag cccaaccaca 29580ttatctgttt acttaaaatc tgattttgtc tttcaactgc ttatatgtca catgatcaaa 29640ggattccttc tatatctagt caccctgtca gacttaggct tggtgaactg cttccctgtt 29700ttctttgcct ctctaacttt aggccgtttt cattgtctgt ttagtttcta gggatcagag 29760ggccactgtt caatcttttc gacctagctc ttccagactt caacagaaat tctactttta 29820tcccccccca cccctttttt aaaacttaca ttctcatatg tgagaacatt ataacataat 29880gtccttgatc cccccttccc cagcccaacc gccactttca accctcaaat cctgccttat 29940tcttaggaag tgattttgcc tttccaccga ttgggtaagt taaggccttc tgacaaggat 30000ctcgtttttt tcttccctca cctaaggtat gcaccgtacc tccttcaccg cccccaccaa 30060cccccgtttg attgattgtt tattctgtct cctttgcagg tttctcttct tttggccact 30120gatgttggga ttcttcgcag ccatccctgg ccctctttct ttcctctatt ttcatggtgt 30180tttcttctaa gcccaaagtt taagtaactt tgccatgata acccaagttt atgtctccag 30240ctcagacttc tttgacccat atatccagct gcctattcaa cattgccatg caaatgtcaa 30300actcattata ttcaaactga atttgtgatt ttacccctgg gacctgaccc tcttccaatg 30360atctctttct catgattagt accactcacc atacatttat tacataagct ggaaatctag 30420atgtccttct tagcctaaat agccctttct tgatatctcc cataccccaa aaattctttt 30480ccattaccac cctaggcaaa gaattactat gttttgtgga aaaatagcca cctagctggt 30540ttctacatgt ttttatttcc aaatttaaaa tcctttttta aaaaacccat tttgattgct 30600tttaggataa agtctaaaat ctttttaaca gtttcaaaac ttaaaaaaac tttgacctat 30660ttcctgactc ctttatagtt tcattatcac aagaactata gtttaacaga gaagtaagca 30720aattttattt gataacttct cacttttctt attgtcattt ttcagtcatt ctggtgtctt 30780gaacctttct cacttccctt gctacaagta tttgtctttg ctggtaacag ttgaaggtgt 30840gaaataactt actctgttgc tttccgcttt cttttggcta tttgtaataa agcagtgcag 30900ggtttgggga gcatttccta agcatgagac aggatatgaa taaaagagaa tgtgattcac 30960tacctctgaa agattgctgc tggcttatcc tgcataattc catcaaattg ttcagtaatt 31020tacttctctg tttcaggatt taggattatt tttgtttttg tcagcttttt tttaaaaaaa 31080aaaccattaa agataatggc ataaaaacaa acttagtaca ttgtgatttt tattcttttg 31140gattctttcc ttagaatatg ttctcaggag ttgaattaat tgttcagggt gtagaatcac 31200agagttttcc tgttagtgcc ttagttcttc ctttgaagta catgttatat tcatccccct 31260ttcccgcttt ttttccattg tcacaatctt gatccaaaca ttcattttaa acctgtgttt 31320gattctttct tggcccttgc tcagcttttt acattatact gtgagacaaa tttgaaaata 31380tattcattgt aagacttact aaaatctacg gaaattcttg gttgctttct aaagctttcc 31440atagaaattc aaattcatta gatgattcca gaattgtgtt ttctgtattc attccttgaa 31500gacatttata ggccactgtg caaggtgctg gggataccta ggtgagcaaa agagtcttac 31560agcaccaaag attaatgaag tcctctttag gaaagctatg cttaagctaa gaagtgaaag 31620atgagttacc tgagtaatgg attaaacact cttctttctg tttgtggaga agtaattagg 31680agaggtggcg gcaagagtgg aaataaaacc aatttataca gcattatctg aagtgcttga 31740aattagtaga atgacggaaa catacatatc attccattga ttccactatc tcaggtattc 31800agtgaaaatg gtatgcttca gttttttcac gatagggagc tagtgtattt tccatttata 31860cagagttaat gagctagatt tatattgtat gggttcatag aacataccaa tgtctttgtt 31920gtcagcagac tattttgctg cctcttaaaa taattttatt tacatacttt tgaaaaatta 31980actactagag ttatagttca ctttctaatt ttactatatt tttgagcttt ccatatttgt 32040catattactt ttcagttttt tcctatgtaa attatttcca atttagatcc cacgtatgtt 32100taaaaatatt tttattaact tccaagtatt ttgaagtttt cactagtaca aagtataatg 32160aagaaaagat gctatattag tgtgggtttt tttcagaaag tgaaatttta aaaatatttt 32220tttaaatatg ataggcaaat tttaagttac aagtcctaat ttttataaaa tatttttatt 32280ataaatctat ttaatatttt gctgatgaat gaagtcccta agtctggaaa tcaccggcct 32340tattgtactg atactgctct ggagtatgtg accagttaga ttgtattact cttgatcaac 32400acatacctct ttaattttca atgggagact ttttcaaaat atgtcaactc acttttccta 32460aaactagctc attttcttta attccagtct ctcctttaag cttatgtcag agacctgcca 32520attgaattgt atacattggt tgtgacatga gagaatagtg gtttgctaat ctcaactact 32580ttttaataga atcatttaat ttcaaaattt actggctact tcagagaacc tgtgtgactt 32640ttacctatgt gttaaaacca attaaaccag ggccaaagga tggcctttat tgtgttattg 32700tgtttgtctg tgtaattagg aattttccaa gctctaataa ggcctgctac attttaaagt 32760agcaaaattt cagaatatca gaagatttca gactatttgc agttggtaaa cagtggaatt 32820taatgcttgg ttgttactga gcccaccctt cccagaagaa ttacgattaa ctgtagagga 32880aagtccttta aaacttggtt atagcagaag tttgagatgt tttccaccaa tactttctat 32940gaattaaata agtcatgtag ggcaatttgg aattgaaaag agctagaagt acagccatat 33000gacgttacaa ctaagttctc tgtattttct gtggtgattt ctaatatgct ttctaaagac 33060tagtaattta ttagatgggt aggcagccat tgttgattag atgttttttg ttgtctggag 33120atttgggcat gatgtgttta ttatttaaat caattgtcat agggtaacaa tttgtgccct 33180cttttttaag gatatggtgg tggttttaat gaaagagaaa atgttgaata tatagaaaga 33240gaagaatctg atggtgaata tgatgaggta agctatattt tggtgttcag gttgaatata 33300aattagaaaa acagaaaaaa ttcttaaatg caaaggaaaa caaacttttt tttaagtgca 33360atttgagtct tcagcaactg atcattttca ggaaatcata aatatctaaa ataatttttt 33420agaaaatgta ttaagtttaa tgcattatca gctgacaagc attttgaaat tgtttttctc 33480tagtttggac gtaaaaagaa aaaatacaga gggaaagcag ttggtcctgc atctatatta 33540aaggaagttg aagataaaga atcagaggga gaagaagagg atgaggatga agatctttct 33600aaatataagt tagatgaggt gaacaatttt cttgtcctat ttcccttttg tcttattgga 33660gttataccat aacatgaatg cttttgacag cctcagacac ttggtgaaaa tatattctgt 33720gttttcattt gttctcacaa aggttttgga tttccactct gccaagtact atttgtactt 33780ggaacactac tgttctggag atacagtggt gaccgaaaca gaaccagttt tctcttacac 33840agttctagag gaggaaatat ataaataggt aattttgata gcagtaacgt tatgaagaca 33900catgatgtga taacagttta aaagcaactt tggttttgcc gatggataat gtctaaggag 33960gtgaagtttg agctgaaact tgaatgatag gaaggagcca gcaataagag ccgaggtgag 34020agcacattta ggcagagaac agcaaatacg ggttccctaa ataggagata agccagcagc 34080cttcaaaaat accatacata ggccagtata gttagagcag aggggagaat gagatgaggg 34140ggacagcaca catttcaggt ctggtgatac ttgttctgtc aggccagagt gtaagtttta 34200tgccatgtta agggtacctt agagcctgat cataagaggt cttggaatgc aactaaagaa 34260ataataagcc agttttgaag tttttaagaa aaaagtgaga tgtgtttatc tgtcctgtgt 34320ttcagagtga tgactgtcag tatattgaga tactagagag gcagaatgga tcttcagatt 34380tgaggagacc agctaagagg ttactataat atgctagaga ataaggaaca gagtgtctaa 34440gactaaaaat ggtgatttca aggactttta aggaggtgga cttgaaaatt cttgggtgat 34500gcataagtgt gacagaaaaa gtgtgtatgg cctccaggtt tatgtctgtg caatacctga 34560gacaaagttg tcaagtctct atttgagatg agtttagtag gtgaatttta gaattcagaa 34620ttaatttgct gagatacctg tgatatatgc atatggaggt gctcagtaca cctaactgtt 34680ttatctttcc tggtaggtaa cactaatatc taaatgtcta attataattg aatttttgtt 34740aggatgagga tgaagatgac gctgatctct caaaatataa tcttgatgcc agtgaagaag 34800aagatagtaa taaaaagaaa tctaatagac gaagtcgctc aaagtctcga tcttcacatt 34860cacgatcttc atcacgctca tcctccccct caagttcaag gtctaggtcc aggtaaacgg 34920gtacaggtca agggtaacac cagtcaaaat gtcagtatct aactttttta tttataaatt 34980ttgtttttct tgacgaaata aactttcctt tttattttaa aaatttaatt caaatcaact 35040tttaaaataa tttttcagct tagtaccata cagttgttgt ccagtatttt tagaaaagta 35100aagtcttagc atgaaacttc cttaacctat tttagcctca tgcttttgac taaaatagac 35160ttaaaaactg aattaaattt ttatatacac ttatatttaa ttaatctaaa gagatagctt 35220acatatagaa attaagtaaa ggggagatct gtattttcat tcaagatgtt aaaggctagc 35280acagaaattt tctaggcatt aaatcattgc ttattacata atgtttttaa tagtttgctt 35340tgatatcagt attgtgaata acggagcaaa ttcagccata ttagttctgt tgacttcatg 35400ctcataattt tgagaagtcc aggcgtaaac taaaactctt ttggtgtact tgaatattgt 35460ttgttgatgt aaaatatgta atctctgatt ttatagttaa caataaaaat aaaattttag 35520gtgagtgaaa gcagtgattt aaatttaaca gtgtcctctt gaaaaacctt aatcacagtt 35580aagtgatggc atgggcaatg aattttaatc atatttagga ataaaatact agatattagt 35640gtataacaat tagatatatg ttaattgagc actacaaagc ctgaaagctt aacaaagtgg 35700ggctataact agtaattttt tggtaaaaat atagtgatct ttttctacag ggtattatta 35760taagggacaa attatagttg taaattagat taatgaattt tgtgagtttt aaaatttgta 35820cgaaccatgt ctcccttgtg tcatattttc caataatata attacctaac aatgacagaa 35880ttatacattt tccctttaat tctctaagca ttgagtaaac agaaaaatat agggatgaaa 35940tacacacatg ccaacattca aaactttgta ctaactagaa acaaaaatag aaggggattt 36000ataaaggaaa atgatggaga cattttagag aaattaagta taaatcgcaa cattctgttt 36060tcttggatgt attctgggta gcttctagtt acttgtaaac aaagtattca ttgtttttgt 36120aataaagtga taatgttttg ttttatagtt gagtggcttt agggtaattt aatcacctgg 36180aaagtgatat ttttttaatt accttttgtt actaaatctt atctaattta aaatactatg 36240ttttgaaagt atatatcttg tatagttatc tttttatatg aaaatgaact ctaatacata 36300tctttctttc agtgttcata tcacaaagct caattaaatg gtcctggaaa aaaaaaatcg 36360ttccatgttg ttccaggtcc cgttcaagaa gttcttccag ttcgcagtca agatctcgtt 36420ccagttccag agaacgttcg agatctcgtg ggtcgaaatc aaggtgaatg aggtagcatc 36480tctctgtaaa gcagagagaa aaatatttaa aggctgcaga aggtgttgct gctttttttc 36540cccttatttt gcttctactt gtcgatttta ttttagcatt agaaaactaa tgcacttgcc 36600tcagcttaat tttctcacca tgaaatgttt ccattgccag cagtgtgcca agacattccc 36660atgattgaaa tcaggtggct attttgcaat atgcagcgtt tattattttg gcactaagat 36720gttatgaagt aggaagatga agccaagtct tatcgccaca tctctagtat attaagatta 36780tatatggcca ggcacagtga ttcatgccta taatcccagc actttgggaa gctgaggtgg 36840gaggatcact tgaggccagg agttcaaggt tgtagtgaac cttgatcaca gcactgtaat 36900ccagcctggg tgacagagga gtgtctcaaa aaaaaaaaaa attaatagta gttcaaataa 36960aattacatat gtaaataaaa agaaaaattt tcttcattat taaaaggaag aaaaataacc 37020ctgcctcaaa acaggtcctg ttttgcacgg cagttgtttc aatattttca gaaacaaatt 37080tgccatttat atcagataaa gttactagag tactgtatat acattttcct ttctaatttt 37140acacacgcta ttatttatag agcacaaaac actcaaaact aaatcaggaa gtatagtcgg 37200ttagttgaga taggatgtgg tcttaagtag taaacagtgt tctaaagttt caggttgcat 37260gtagtcacat ctgtagaggg aaacataatt tgaaagatgc tcttgcagga aatttttctt 37320tgtttagtca aaatatgctc tctttgtttt taagttaatt tattatgacc taaaggcatc 37380taaaaaataa ataagaaaat gaaattccat atttgtggaa attcatattg aagtctaact 37440gtggctgttt tggtaaaagt atttgctgct ttgaatcaaa tttagcatta aagggctgcc 37500acagtataat catgttaagg agaaaagaat gttgtgcaca cttgcattat aagccaataa 37560gaaatagata acaaggccag gctcagtggc tcacgcctgt aatcccaata ccttgggagg 37620ccaaggtggg cgggctgctt gagcccagga gttcaagacc agcctgggca atatagtgag 37680acctcgtcta ctaaaaataa aaattaaaac aaccagccag gcatggtggt gtgttcttat 37740aggctgaggt ggaaggatca ctggagccct ggagattaag ggtgcagtga gccatgctta 37800tgctactgca ccacagcctg gggaacagag caagatcctg tctcaaaaaa aaaaaaaaaa 37860atagaagtag agagcaaatt attttttatt gttaccatta tttagtaaca atatttaagc 37920gctttatagg taaaaatcct taagttctgg tattcccttt gggaaccatt ataattaagg 37980tttacagaac acccatcata ttcttcccaa acattttttc attgcttgta actttccaaa 38040gaattctcct tttgggcttt ctggttttat tttaagccca aaggtgaatt ttttgggaag 38100tttgagctaa attccttcaa ccaaaatata caagtgaaga aaaaaaattt gtatttaaac 38160atttgcacat ttacttctac ctgaagcatg tgaatgtcaa cacttcatat ttcataagta 38220gtgggttttc ataaaaatag tattgtaaat ataaatcatc ttaaagttag tttttatttc 38280ttcttgaata tttagcagtt aagtacagtt actagtcttt tgttgctgtg ccatatacct 38340ggtttttcca taacagttta atgagttgaa taattccaca tggaattatg tttattttcc 38400tcaggaagtg gtatataaga tacatatttt aatcctcaaa tggaaggatg tagttcctaa 38460acgtaaagtc ctgttaatgc agttatctgc tgattttata atactataat atttgtgtat 38520actttgaaaa gccttaaaaa aagtttaaat tctattttga tatagtaagc agggttttaa 38580ttacacatga tagaatttta aagagacaaa attaaaatgt ttaagtagaa tgttttcatg 38640gttgatccag aagtcttctg ttgaataagc agtaagtatt cttccttata aaattttcat 38700agttgttata aatcctgttt atttttggag ctatctgata actaaataat cttccttatt 38760tcttctttta atgaaatgtt tgatttgttt ctcaatcaat gaagatccag ctccaggtcc 38820cacaggggct cttcttcccc acgaaaaaga tcttattcaa gttcatcatc ttctcctgag 38880aggaacagaa agagaagtcg ttctagatct tcttcatctg gtgatcgcaa aaaaagacga 38940acaagatcac ggtcacccga aaggtttggg tttctgtaga aataagaatg ggtgttctta 39000agtgtgttta atagataaag taaactttcc tagtcaaggg ttagattttt attatctctt 39060gtgttccgac tttctacttt tcaactttga acttcaaaaa aacattactt tgcttatcct 39120ttgtactttg atcaggttgt ttagaattgt agatcaaacc attctttgat cattttattg 39180tttaaatttt tagttccatt tataattttt atagccaact ctcggttatt tctgtctttt 39240gagattgcaa ttcagaagct gtatgtcgaa gtaatttatg agttgacttt tatacttagg 39300cttctttaaa tactaatagt caagaattct agagcatcta ataaaaaatt aactttcaga 39360tcattgggaa tctgtcctca tttaaatatg tgtaaatgca tttccacagc aaattgcttc 39420atgccctttg tctataagga aattattcct tgtagctaat acatttttca ttttgcagtc 39480caaatctttt ttgagaaagg cctatattag gttactttat ctgtactgac acttttaata 39540ttggaagaat gcaactgtca tctgtcagcc ttttctgcaa caggctaatt actcagtttc 39600attaattttc tctttgttcc caattatata gtcattttaa tggctttatt tgtagtcttt 39660gcaaattttt catattttat tagcttgtga aaataaagat tatgtaaagt ctattaattg 39720ctgggttcag tggtaggatt atctcatgat tcctacttgt acagaatgat tagttttgct 39780tcttcacatt ttggagttta gatgttaaga tttcacacag tcttgtttct ctgcatttgt 39840ttctttgtaa aaaaaattac tctgtttttg agtggaattc attctgttgc tttcttacca 39900cttctgcaaa tttttatctt agccacatat aatcccccta gtttgttgtc atctaaactt 39960aatgaacatg ttctctattc cataagccag gtgattggtg aaaacactaa acaaccctga 40020gcccagggcc aacccctacg gaacaccact actttaccca ggttgatatt gacttattca 40080tactagctgc atgaatataa ccctatagca aattgtacaa tcatcttgtg tgacctagat 40140ttccaaaact tatttatgag tatattttct catacacaga agccttgctg tatctttttt 40200acataataga cttcttacac tttctacacc gtgtcacagg gattggttta gtcacacttc 40260cattaccaag tttatatgct tttgttgtac attttctgta attttacaca tttttgcaag 40320gcttttcttt gatatcttaa ataattgaga ggtggctata ttttgaaatg agattctttt 40380gaaattgtag agaatttttt attgtattta tatgataact gggctcatat ccaaagtcta 40440aacatcagat aattttatgt gtgtaagttc ctatctcaat ttttaatttt tttgctagca 40500tcatggtact gaattttgta tactttcaca gtcttaagaa tgaatgctga gatttagctc 40560tagcttacta gaatgcctac tctctccatt atgtcttaca gacgccacag gtcatcatct 40620ggatcatccc attctggttc ccgttcaagt tcaaaaaaga aataatgtat taaaatttac 40680atcttaaaaa aatccagtac agtgcatgaa gcatattttt aaagaagttg gtgtcttact 40740tggtcagaag tgctaaatct gctagtagag gtgcatgcct ttcattgctt ttcaaaacaa 40800tacagctgtg tttatttgtg aagttaaaag taaatagcat tttaagccat aatgtcccaa 40860aatagatgtt ctgtcattca ttatttacaa ccatttgctt catttaaaac catttcagct 40920ataacaaagt actttgcttc ctaatttaaa cccatttttg tcatttccaa atacatcctg 40980tccattggct aagacaggat tacctaggct tgcctgaact ttgggcatgg aagaaagact 41040ggaaactagt tggaaacaac atacttatgg aaaagaaagt cagccttttt atgctgttaa 41100cagatgtcag agtgattctc accaaaaaaa gttaaactat gttgtaagca gccaactgta 41160aatgtctatt ttgaaattcc ctatgctaag ggtgccttag aatcattgtg tctcttttat 41220ccagctttac ttttttgctc cacatttaat gcaaaagaat cttgtgatgt ctacaaggag 41280agaagtggga tatattttcc tttctgacac ataatttggg gcaaaaaaga aagtcttaag 41340agtttatctt ttctctgctg atacggttgc ttgataaaga catctcaaaa tgtttaccaa 41400tgttttaaga agctttgtgt gatattcttc caaatgtagt taccaaatat aatatggtag 41460aaaaggctaa atcatactta atgagcaaat tgaagtaagc ttttaaagta tatttctctt 41520ttggtgaaag gccaatggag acattgtgaa tttaagtgaa catttgcctc aagatgttaa 41580ctataaacac actgcataca attttcttct gaataacaaa tgaatgctta ttgctgcatg 41640atgtaagcaa aagtcattat ttttcctatt catttgaaat aagttatggc ttaaaatgct 41700tttggagttt atttctcaaa attaaaatct ggtcacatga gctttagttt gttttctggt 41760ttaaaaaata aaaaggtttc tcttaacagt atttccagtg acaatgcaag gtaagtatat 41820caaaggaaat caacagttgt gcttgggggc tttttgttat gggatattga tttcttgttt 41880tttttccgta acattgtctg ctgcaattta aataaaaatt acgacatttt aagatatttc 41940atagacaaac caaacaaaaa tatatgtttt tactttaaag tgaatgtttt tctcttcagc 42000tgatctaaaa atgaaagcaa gatatcttat gtagaaatat tttgataata tttttacagt 42060gagctttccc atgtttttat gtcttaagtt tctttgctgc gtttatgtag gttgcacaag 42120aacttttact cacttgtaat tgtgcctcag actttttgaa agtctacctt ctaaattgcc 42180ccgacgatct agattctaca tgttaccatt ggtttattct tgtgctttct gtatttaaaa 42240ctttggctgt actaagcaaa tgcaaggtta taatttagct aatagtagtt tacagacaat 42300tctgatgatt atgatttcat ttggtttaac taagctgtac tagttcattt cataaggaaa 42360tgatactgta gacaaatgta aataaagcct gtgagtcaag catcaagtgg tgtttgttag 42420aaataaacta gagattttta aactctgatc taatgttcat ttttaaacta attacagttt 42480tctgttacct ttgatttgtt aattttttac actctttata ttcacctacc ttttatatat 42540gagtgcttgg tattttaaat tccaaagcca ttatcactgc aacctcagaa tttttaattt 42600aacctttttt tcgttttaaa tagagagctc ctaaattagt tgcacacttc ttgccagaac 42660attacatccc tcattccatg tggtaaaata ccatatcgta gtgtgcttcc tgaattactc 42720ttaaactgtc accttatata aagcactcaa ctgtcagctt aacagctaag ttttcttaag 42780tttctatttg gagtcctttc atctaaacaa tattgaatga tatctggtaa taaatgtcca 42840agttcagaac tagatctaaa tagaagatca tctctaggca caattaccaa gctaaattag 42900atctctgaaa ccatgtcatc tccccttctt gctatcttgt gtacatagcc agtgttgtgg 42960atgttggtaa gaaggtaggg aatttctcca ctttgccccc tgtttttgac ataaggttta 43020aatgtttttt aaatagaaat accctcatgg ctttcctgac ctggaaatgg tatttctatt 43080tctggggaaa ttgagtagac aggtttaact aaaagacagt ttcgtgtgtg tgtgtgtgtg 43140tgtgtgtgtg tgtgagagag agagagagat ttgcaggcat tatctaattc tccctatgat 43200cttgtgagag tatatactct tgcatatctt gtgttttgcg aaaaggaggg ttaaagcacg 43260gggaaattaa aaacttgccc aaggaagcca caggaatagc aaagtaggga ttcaccccca 43320aacatgatgg gaaatttcag tattatgtgt aataaaagcc aaaactcttt tgagaaaata 43380aatgtgaaat tgttaagccc gatagtcaga tattttttag tctggtaatt tggtagagaa 43440tatataattt cagccaatta tagtcaggga ggttagacaa acactatttt ggataacttt 43500agatttaaag aaaagttgca aagattgtgc agacagttct catatatccc cactaatttt 43560ctacagtcat tagcatctta aattgccatg tgtgtatatt tctcaaaacc aaaaccagcg 43620ttggtatgtt actattaatt acattacaga ttttactaga atgttatcat ttttcccctt 43680aagatattcc ataatctaga gtaccatctt gtactactaa taccttcctg ctctcttctc 43740ctctatgaca gtttttcatt ctttcctcat ttttgatgat tttgacaatc ttaagagagt 43800actagccagg tatcctctag aatatccacc actgcattct gtatttgact ggtttttctc 43860acgattagac tgggattatg gggttttgca gagaatacca cagaggaaaa gtgtctttct 43920tgtcacttcc tacacagagg aaaagtgtct ttcttgtcac ttcctgtgca gggtacatca 43980taaccctgtg acttcactgg gaatgttaac attcgtcatt tggttaaggt agggtttgcc 44040ctgattcacc attgcagtta ctgtttctcc ttttacctac tgttactctt tcaaatcagg 44100ttatactaag tgtagtccat catcatgata agacggggga attaagttcc atatcctttt 44160ggggttaggt atctacacag tatttggaat ttttctataa gaaagatttg tctccatttc 44220ttttatatat atactcagtt atatttattt tatacttgtt acttattttt catattggct 44280tttgtgacct ttgacaagcc tttattctct gttagagggg aaagtagatg gagagaggga 44340gggaagaatt tttggagagg tatcaaatag gagaaaacat ggggtctaga gccatgacaa 44400ggcggtgggg ggtgccttca atattaaatg agagcctcca cagttaatta cagtatgagg 44460agggatgggt ccagattgca ttacagccaa atttgtagtc attgagagtg agcagtaaac 44520atttaatggt tttttttttt cccctcagcc acatcgaact acctggctgc ttgaataagg 44580gtgttgtaat attacacaca gatataaata ataaacatgt agtaatagtt cttaaatatt 44640tgtttagcaa aataaaaggt gctgggataa gaaaaatgtt aacattaact tggtaaattt 44700gaaatacttc aatgcagatt aaaaggtatc taaaaagatt ttgaggaaag ctacacaatt 44760tttttcaaag aaaaatacct aaatttgaag ctaaaaatgc ataggacaac tcctcccctt 44820aacctcaatt tagtgcgaga aaagctctta taaaatacag ttgtacttaa gtgtctgtgg 44880ggaattggtt ccaggatcac ccacagataa caaaatttca agggtgctcg cgttccttat 44940gtaaaacagc atggtatttg catgtaacct atgtatatcc tctcatatac tttaaatcat 45000ctctagagta accctaatac agtgccatac attacttcac gtagattcag cataacacca 45060tgtggcaaat tcaagtattg tctttgggac tttatagggt tttcttttcc cctgaatatt 45120tttaatttgc agttgttgaa tccatggatg ctggatacag aactcatgaa tatagagggc 45180ctgttcttca ggttagagat aaagcctcca ataaaatagg aacagttttg cccgaataaa 45240gaggcaaact cttaatgagt tgacttgcag tcttgaagga caaatttata gatgaataag 45300gcaagctaag gttattctct ttgcaacact gaagatttga cgtttttagg gaactaaaaa 45360taccatggct gtgggaaacc tcggagaagg acagggaact tcgtaataat gctgttggaa 45420ttaaactgga ccacagtgaa tactgactac agaatattga taatttgaat agctaagtgt 45480tgaacacaga acagtgggat tttgcaccta ccctaaaatg aaacatctgt gacctgttat 45540tggtgaggtt atcacaaagg ctaagtacct ccaacctaaa agtaatagga actaaagttt 45600actttaatgg gtgtgatgca gtggtagagt tgtgactgat atttggttac tatctggttc 45660ttccactgac ttcaatttag ttaatggtaa ctgagtgact cgagacattc tttatgccta 45720agttgttaac acctataaaa tgaaaatgac cgtttctacc ttgtgggaat tattataaat 45780aatgtagcat atatgtatgc agcaaatatt ggcagttttt atcccttctc catgtttaca 45840gatatttctc atatatacac acacatgcac atatatatgt gtatatatgt ttatatatac 45900atatatttgt ttatacatat atatgtttat atatacatat atttgtttat acatatatat 45960gtttatatat acattatata tataataata tatatgtata tatatatgca tgtgtgtgta 46020tatattctca gatgcctatt tcccacttac tctttctaga gcaccagatc catgtatcta 46080gctgcctcct gtgtatctcc ctttattttt tttctttttg tcacgatgag tcttgcggtg 46140ttgcacaggc tggtctcgaa ttcctggact caagcagtct tcctgtcttg gcctcccaaa 46200gtgctaggat tataggtgtg agccactacg cctggcctgt atctcccttc acatgtcaga 46260cagccaattt tactgtattt aaattcttcc taaatcctct attcaccaaa cctgtatctc 46320atgttgtatt tcatattgat aaacatcatt atcagtactt gataatcctc ttagatttcc 46380tttttgtgaa cactaccact gctttgattt gtgggctagg atattgtagt ttcttcctaa 46440atggtcattt ggcatttagt aggatttcag taatatttgc taaatgaatt tttttcacca 46500agcattgatc acttcatcac tgatgctaga atgctcttaa ctaacgagca tttcatttca 46560tgttatttct ttgtttaaac cctttcagtg ccttttcatg gctttcagaa ttcaactaca 46620atccttatgc atatagtaaa aagttcaggg gttttttcca ggactcacac tacccccacc 46680tcccaattct aacctactta ggtgcacaga acctatacac tagctacgtt aactatgctg 46740aactactaat aggctttcat cttagtacaa gtttcttata cctgggatac cctacacata 46800ccccactacc atttcttttt tcatttagct ttcatgactc aattcagaac actaggatgc 46860catccctgat gcctcttccc tttcaatggg aattgggtga aactcctaaa agtttctcct 46920tccccagtat ccctctccct tagcatagtg tttgtcatta tagcaagcac tcaaatgttt 46980gatgagggtc aatatctgtt acctttctca aagaggtttt gatcagttgt attttttcct 47040cattactgat agaataaatg ttcttcgctg gaaagcattc tttgattgtg aaaggagttt 47100ttattctgaa attaaagctt tgtaagacca ttgtaggaga aacttttctt ttttcttttt 47160aaatatttat tttagagaca ggtctcacta tgttggccag gctggtctta aactcctaac 47220cttaagtgat cctcctccag cctcccaaag tgcggggatt ataggcatga accaccatgc 47280ccagcttttt tctgtttctt aagtccaagg aggccatttc ttcataagct gaaaaaactt 47340tgaagttatc tgtccagcca tccttcagga gagggtagaa catgtaaata aaccttattg 47400tcctcaattt tctacttaaa gtatgccaac ttatatttta ttacacacaa ctaagatagg 47460ttgagaaaga gcagcctcac tggcatacat tttgaaatta gattatttgt atatcaccaa 47520ggacagaagc acaaaaaatt actatgctgg aagtaagact tggggtcaac tattggcttt 47580cttcctaaca tgctgtgtga tcctctgcaa aaatattaac ctaagggact tcactatgga 47640ttggtaaatt gagttcccta taaatgtatg atcctgaagt aagtgcattg gggtgcatca 47700ttggcaaaat aagcaacaaa tcaaaaagta atttacctaa cttggaggag cagatgatag 47760agttcaaaat gctttgttag gttaaaatac tacgaacagg catatcatgt actaggtaga 47820accgaatttt ataagtatag ttaaaaagct ttcaaacaag aaaatataaa atacattatt 47880gcttgataac tagaccagta gatttttact gataaattgt ttggacattt attttggctt 47940atatacataa cactaactgt aaatgagcta tttctgtgaa aacacctaaa agtcaaaact 48000aaaatggaag tgttcagttt ggatgaagca aatccagtta attatggtct atcctcgtat 48060taactcttag tgacactgtt acctctaatc tagctgttga ctgtgaacct gtcaaaaatg 48120ttgctttttc agcaattatt tccatgtatc aaaattctga tcagggaaaa caagttatct 48180gtattcttat aatttgaata atgtattaga gcctaagaat tttaatgtgg tatcagcaac 48240tgtgacacaa gattgtgttg ccctatatca cactcacaat tatgtctcct gttccttatt 48300attgattcct tagaatcaca aaaagggaaa ggttaaggag aatgtagtta attctaccag 48360aagaaactac ctcagtattc tcaggctgca cccatagtac ctgtggcact aaaatcttag 48420gatagaagag tgctaagaag gaagaaagaa taataaaaat aatgttttgt gttttttcct 48480catattagtg ataatgaaaa tgtgtttaaa ttcaggcctc ccataaagta ttgcgtatca 48540taaagtctaa ggcaggtacc tgaccgtgaa aaactacatt ttgaagcact gatgactgat 48600aagagagaca caaaatgttt aacctgtaat tttggtgaag ccattggaaa ttagcaaacc 48660tacactatgt tttctctaca tttggagact attcatctgt taatgtagat gcaaaagaaa 48720gtcttattca aggactccaa agacatattt aacacaatat taaacattga acctgcattt 48780taagcaccac tgcattctgg tatcattgct tagtgccctt ttgattatat ttgctggggc 48840tatttgaatc agtatgatct atttttgtaa aatccttaat ttttttaatt agaggataag 48900ttagcacggg ccttttagag ggtaatttga gagtatctga aaaattagag tattcacagt 48960atgaacattt aaacatacta atgaattacg ttactgcatg ctaggtagtg aactaacatg 49020agataccttg attgaagcaa ggagaacaat tccgattgct actgtgaagg atataaaagg 49080ggttggtgta taaaactact cttaggacag taagtataag accttggggt caggcaaatt 49140gtacttggta actgaatttt taagagttca gtgccgcggg gctaagtctg ctcctggata 49200tgatgagaag ccagtaaatg gcttgggaaa aaaatggtct aagacagagt tgtagagtta 49260gaggacctgt catacaaacc ttgtaagcca tagaagggaa tttacatggt atgcgaagaa 49320cagttggaaa atagtaaaca gtttgaagtg acctagtttt aatacaaatt ggccactgct 49380tggagaatag atcagaaaga accctactgg atgggaaggg aatagtaaga agtcttactt 49440aagcaaatta aagttattac aaatttatat gatcaaaaca ctaaatttta gggtaactac 49500atgacagacc aatagtgttt taagaagttt aaaatgtgga aattcaaaag tggaagtagg 49560taatttgagc tgttgtgaga ttcattcctc agtaaatggt aaaagtacta tttaagtaga 49620ctctaataaa tcatggaacc atattttaat ctccataacc actaagaata ataaaggtat 49680aacaagtagt ggggaaaaag ggagtaatga aaatgattaa tacaaattag ttttttaaga 49740ggtaaagaat gacaaaacag caagccagtt aaactctcgt acctagaggt ggattataca 49800ccaattaatc cagagtatca gacagggtta agataaattg acaaagccaa ttgtgtattt 49860gtaatatgta ttttcagttt ggaaataaga gaatggggaa agtaaaagtt tggaattaaa 49920catccccatg ggtcagcaaa tagtgggatt tttttttgaa tgaaaataca atttatcagg 49980ccgggtgctg tggctcacgc ctgtaatccc agcactttga gaggcgaggc ggatggatca 50040cgaggtcagg agatgaagac catcgtggct aacacggtga aaccccgtct ctactaaaaa 50100cacaaaaaag ccaggcgtgg tggcacgcgc tgtagtccca gctgctcggg aggctgaggc 50160aagagaatcc cttgaacccg ggaggcggag atttcagtga gccgaggtcg caccattgca 50220ctccagcctc tgtgacagag cgagactctg cctcaaaaaa taaaaaaaga aagaaaaaag 50280aaaatacaat ttgtcaacac ctgtgagatt gagctaaagc agtgcttgga gttgtgtaac 50340tttgaaagcc tacattagag aaaattaagt caattaaact ttcaagaata caggaaaata 50400ctggcagatc aaacgtaatt cagagaaaaa aagagcagag gacttctgct tcaggccaag 50460atttaccctt caacctgtaa caaactagac aaaatgtatc aagcagcatt tttcagtaca 50520ttgaatgtaa ggctgtggag aagtacctga gaaaggaaaa caaggtgagt cctattaatg 50580cttcccagac tggccagatt ttctcacagc agccagccct atctcacatg tattaatgtt 50640ataatttcac atactctata atgctatcct ttgattagcg tttagtttga tgggaactcc 50700aaaccattaa agaaaattca ggccagccgt ggtggcgccg tgcctgtgga cccagctact 50760caggaggctg aaatgggagg atggctcaaa cccgagcagt caagtgagcc gttattgtgc 50820cactgcactc cagcctgggc tgtaacagag caagaccccc atctctaaag cagaaaagaa 50880aaaaaaaaaa tcatagcagg tgctacattt gcactgagaa gaatccacaa gcagagatgt 50940ttgtgagaac agtggtgtct tcagcagcat cagttcccgc agactcgagt gaattgggct 51000ccggcatgtg caccacgtgg ggtccagagt gcctggcccc agaacagccg ctgagtgcag 51060ggaccctaag gacaggggtg ggggtctggg tgacaaggcc ggaggagtcc ctggcacccc 51120tcacccaccc cgcgggacac cccagttgac accgtgtgcc agcaaagttg ggacctgatg 51180gttttgggaa cgtaggtcat tgaaagtggc ctgcagcaca cagcctgtag ggagcaggca 51240aggagggtcc ttgtccatct acctcctgag gctgtccttc ccgtggccat gccacctagg 51300caggagtgcg taggacttgc ctggctggaa cccacccttg aaaaccgcct gaggaggtgc 51360cagagtgtgg ctgcgggctc cagaccacgt cggctccacc tgcccggaac ccagctgcca 51420ctagcgctgg ctgggaacac aggcttgctg tcttctccgg gtcttgggaa acctgtactt 51480cttaagactc attcaggatc agcatgtcca tcccaaggac ttcttcagga aaggcactcc 51540ctccccacta aaagaacgtt cctcaaagca atttgagaag cgcagcccat ccgcctccag 51600ctgggtggcc gcagacacat caggctccgg cctccctgta ttttcatgcc ccacagtgaa 51660aaacgtgggc atttctttcc agttatgtcc ttagccaaat gcggagcttt tctgcccagg 51720atgggcaaaa ttctagcact ggcaggggtg caacccaagg ggtggccggg gttctcactg 51780cccttggcca tggggcaggc tggtctagac agcaggggga tgttttcagg gttggagttt 51840ccccccacct agtcctgaac ctattgggtc atggagcacc tgtgttccca tgaggccagt 51900gtgggccaca catgacttca aacggccctt gaggcttgat taagtgtgcc agtttcattc 51960caaacatgct gctctaaagc atccctgctg agccacagga ggatcagata ctgtgagctt 52020ctggcctgtt gatactcaag agccagggca tgcggggaag actccagaag tcctaactct 52080tgctaacctt acatctggga ttcttccacc ctcagctgaa gaacttgatg aatttcaact 52140ttgaagagat tcttctaaca cttggccatt tcaaccaggc tttgctaaag tcctctgaaa 52200ttgggggact gaaagccaat tacttagatc acaggttgcc tgtgaaatct gcaaggacct 52260gctgtcaggg ctcattaaac cccaaagtat ggcccctttg gcatgtggac tactctgacc 52320cagaggagac tggaagatct cagaagccta gcctttgtcc ttccctcctg gttcctgtgc 52380ccttttctcc cacaaagtga gtcacagaaa ccagaatttg ttttccccaa ggcaggtcat 52440agaaactaga actcctctct cccaaacata gccataaaac ctagcaaggt cactctgtcc 52500cttctccctg gaaaacccct cattcccgag gggtcctgcc ccatacccag gggaagggga 52560attgtacaaa gcaaccttgt tgggtttccc cactccgtcc atcactgtta ggccacgtta 52620gcgtgggtga cagagtgaga ctgtctcttt aaaacaaata aaacaataga atttctgtgc 52680ctactaacta aaagagataa tcacacgttg ttcaatacaa caacagcaac aacaaagctg 52740taggagtact tgcagtaatg atagaggaga aaagatgtca caaggaaaaa tcctctttta 52800atctcagagg tttatagttt taacaggtac atttaagtct attatctatt ttgttttgtg 52860gtgtaaggtc tatgttcatt tttatatcca tacagctatc tagtttttcc agcaccactt 52920gttaaaaaga ctgttctcct gaatcacctt aacacttttg acaaaaatct gttgaatgtc 52980tgcatatgtg tacacatatc tatatctcta tatgtgtata tatgatgctc tctataatac 53040tctctttcat tgatctatag atcagtgtct tagtttgttt tgtgctacta taacagtact 53100acatctgggt aatttataaa gaatataaat ttatttctca atgttttgga tgttggggat 53160gttcaaaagc aaggcgccat catctggtct ctggtgaagg ccttcttgcc ctgtcctcac 53220atgtcaggat gacaagaaag ggaccaaact ccctctgtca agcccttttg taagagcagc 53280taatcccatt ccctcaagag atgtcctcat agcccaacca cctcttaaag gcgtcacctc 53340ttaataccat catagtagcc attaagtttc aacacgaatt ttggagaaga cacattcaaa 53400ccatagcatg cctatcttta tgccagtatc acgctgccct gcatataaca atagtaaaat 53460atagttaaat ttgtaaaaac aagtatttga attttattaa actattgcat tggaaggcat 53520tcaaaagcat acagaaatca tagaagagaa aaaaagacct gcaaatgaaa ggagtaaaaa 53580ttatgactta ctggtttttg ggatcgagga tcctgcccat tcttcatgac tatggctatg 53640ggaaatactg gtagaaaaaa aaaaatcata gccttactgg caaaaagttc cagagactgg 53700agttaaggct tgaaaagcca ctgcaaattt aagtaggaaa tcttggtaat gagaaccaga 53760gaagaaatga gatagaacta cccaaattca tgtctcacag caaactctgc atgagtgaag 53820gagaccacat gaagcctgag gaaaaagcaa gcaaagacca gatagaaatc tagtcccagc 53880actttgggag gtcaaagtgg gaggatcatg atgttaggaa atcgagacca tcctggctaa 53940catggtgaaa ccctgtctct actaaaaata caaaaaatta gccaggcgtg gtggtgggcg 54000cctgtagtcc cagctacttg ggaggctgag gcaggagaat ggcgagaatc tgggaagcgg 54060cgcttgcagt gagccaagat tgcaccactg catccaacct ggatgacaga gtgagactcc 54120gtctaaaaaa aaaaaaaaaa aaaaaaaaaa acaaaacaca cacaaaaacc attattcaag 54180aatacatcta ctttgattac tttgatgctt tacttaaggg gatgtattcc tccctgtgct 54240gtgtgtactt tgggtgggag aaagctgaag cttcagaaac tttaaatgtc agggaatagc 54300cctaagctgg cagagtagct gcaaataaga ggagaatccc tagaatcaag gaacccacac 54360agaaggtgat ctccattgct aactgaccag caaattacac aggcataggg aaatcaatct 54420ctaggaagcc aagctaaaag agcatggaaa accatgagaa tgaagcagag ataccaagag 54480cagcatgccc catgggaggc aatattggca gtttgaatcc ggacaagtta actgtcaaac 54540agagcaaaca aacataaaat agataatact cagggaaaaa aacccggtag actccagagt 54600cattacaata tattacaatg tccagttttc aattaaaaaa aacaggcatg taaagaatag 54660gaagaaaaga cagcagaaaa ggcattcata gaaagggact ctttgatcta gcaaagactt 54720ctaggcattg cttttatttt gatacagtaa ttacattgat tgatgtttaa atgttaagtc 54780tacattgcac tgtacttcca tgacaatcat tctgagaaga caaaagccat gggatcagtg 54840tttctcctgt tatttggcac ttagaatcag taattttgca ctaaaatata aaccccatga 54900atataggcac tgttatgtat tgcttttgta tccctaatga ctgaaacagt gtctgacata 54960gattgggtgc tcaatgaatg gttattgaat gaatgaatga ctaaaatata aactaaaatt 55020actggttggc tccatgaaga aagactttca gatattttcc atccatagtg aggcaatcaa 55080aggctgatca tgtgactcat tattaatctt attttgtatc catatttaag atttttaaat 55140agatgtttaa tagaagtaaa tagcaaaata aattcatcat cactagctaa atattgacat 55200acattataca ttttaagctg tagggtacaa ttaagtaatc tgtgaattta aaacatgtcc 55260aggttagaat ttcattgaaa acataatagg tgaatatata ctttaaatta aaaaaaaatg 55320aatatctggt ctttcagttt aatcctctac tggtttattg actgttaaac aaagttgcca 55380atttgtaacc tcaaaggaaa acaaagtgac cacaattatg tactgataag tagatctcta 55440ggaaagaaat cagagattcc ctaagcaaag catccattgg ccctatggaa attctcttta 55500aaacagcata aagcatgtta ttagcctttt ggtcactaga gggtggtgtt gtttcagacc 55560aacacaattc acactatatc cgtagtaact gaccagaaag taccaaccag aatccaactg 55620ccagcttcac actgttactc cacaaaacct cacagtcact tgtttacaag gtgaatagct 55680aaaggcagac aatataggat gaagcaaagt gctgaaagaa gagaaatacc caaattatct 55740gaaatgcaac acattttgtt cattggcata gaggttagtg agaggctaac aattctttac 55800taatacatgc cccgacgctt accatgctta tcaagtccaa ttggctacag caagcacttc 55860agtgggagaa gtaaaagttt caaagaagtc aaatatttgt gaataaaaat gagaatagtt 55920tttgatgtaa ctggaaaaag ataagccttg cttcctttgc tgtgtgaatg gtgatatggt 55980cttaaatttt aaatattgaa ttacactaga attttactaa ctatagatgg tataaacttg 56040atagccactt tcatccaaat gctttggata aagctttgtc taagaataac ctcaaatatt 56100attttatttt caaatgatat taggctagaa aaatactatc ccaaatacag gtttgagatt 56160aattcaaaat aataagacaa aagtttacgg aagattttag aatcaaacca tttcaccctt 56220cctagctgtt gttcccattg taaacgtgac attttagtca gtaaaacgca aaagtgcaaa 56280acatttttaa atgacagcaa aacacaaaat gaaattcatg ttgtgattaa tgcatcttat 56340taaaatggag tttaagttgc attcttgctt ttccttcttt aaaaaacata taatgttata 56400gtgatatttt ttcaaactct catacagaat caagacaaca gcacataaag tatgcagagg 56460gacatatccc attcttacct cttaacagta atttaaaagt taagaacacc catacagaac 56520atcagctaag ctaggaaagg caaaactgat tgggcttgtt ctgaaagttg atctgtgtca 56580atcaaaataa tggtctttag agtgctatat gattaagtct aatttcttaa atatgaggaa 56640aaatgaactt tgcaattatt tttaaaaatc ttttcatctc ttagacagtg taaaaaggca 56700aaaagcaaaa aacacaagac tttcccaaga ttaggggaag atgatctcac cgttaatatt 56760catctgctga gagcagttat gggacccatg tcaggaatac atgcttcaaa taagatggga 56820cttgagaatt gctgagagac ttcaggaaca ttctttaact tcatggggcc tcagttaccc 56880cacctttaag ggaaataata atagcaatag taccttggag gatttctgtg ataaaatgag 56940aaaatgcttg tgagaagtac ataaaatatt ggcttactga aagatgttac gaggcaagca 57000agtatgccta ttttcattca atgtttatta tttcaaaggc aaagtttcca tatagattaa 57060ggccttcata acatctgaat accttttcta gttcttattg tatgaagata agaaggatgg 57120ttggaggctt tgtaaggaat acctttctct tctgctcccc tctcactccc ataggctggg 57180agcagttgat ttcactacct cagatatgca aattgatgcc accttcctgt agccactttt 57240gccatagctg acctttttgt tgggtggtgg ggaggggagc gggcaggatt aggcgagggg 57300tagtagttgg gatttcttgg tcttggaatt caaccatccg atgtatttgt ttccctgtgt 57360ctattccctt aagtctacag gcactgtcta gtaattttat agttttcagc ggcaccccag 57420gggacaggac accttggcct ttttataatc actctagagt cacaaagcag aaaggaaaaa 57480agttaacgat cagctatgca aaggcttgtg tggattgctt aaggacaggc gattttcact 57540ttaccagagt tactaaagga cttcaggaga aggatcccgt ccctgtagga gcgtttgtgg 57600ttgaagaagc aggaggacga gcgctcaggt gcgcctcggc agtttgcctg gggtccgcaa 57660gtccagtgct acttattagc tgcttgccgt ccaccgacac tttagcggag aactggagga 57720gaccccagtg ggctcccggt ccggccagga gcagggcgtt tcagaggaga ccgtctgggg 57780cttcccaacc caggcgatca ggataggcaa cagccaagag aggcgctcag ttgctttgca 57840ccctcctccc acctcggttc tgtccacacc cacgggcgcc ccgccccctc ggccttataa 57900aggggactcg gcacctccgc gcgcctcgcc accagaggtt tcccagagag gaaggcgtgg 57960ctccctcccg ggccagtgag ccctggcgcc gccgcggccg cggtcccagc agcggagtag 58020ggcggcggct gcgccccgca ccatgggggg cagcccagcc ccagccgcgg taaacgccga 58080cctccgccgc cgcccgcgcc gcgtctgccc cctcccgctg cggctctctg gacgccatcc 58140cctcctcacc tcgaagccaa catgaaggag acccggggct acggagggga tgcccccttc 58200tgcacccgcc tcaaccactc ctacacaggc atgtgggcgc ccgagcgttc cgccgaggcg 58260cggggcaacc tcacgcgccc tccagggtct ggcgaggatt gcggatcggt gtccgtggcc 58320ttcccgatca ccatgctgct cactggtttc gtgggcaacg cactggccat gctgctcgtg 58380tcgcgcagct accggcgccg ggagagcaag cgcaagaagt ccttcctgct gtgcatcggc 58440tggctggcgc tcaccgacct ggtcgggcag cttctcacca ccccggtcgt catcgtcgtg 58500tacctgtcca agcagcgttg ggagcacatc gacccgtcgg ggcggctctg cacctttttc 58560gggctgacca tgactgtttt cgggctctcc tcgttgttca tcgccagcgc catggccgtc 58620gagcgggcgc tggccatcag ggcgccgcac tggtatgcga gccacatgaa gacgcgtgcc 58680acccgcgctg tgctgctcgg cgtgtggctg gccgtgctcg ccttcgccct gctgccggtg 58740ctgggcgtgg gccagtacac cgtccagtgg cccgggacgt ggtgcttcat cagcaccggg 58800cgagggggca acgggactag ctcttcgcat aactggggca accttttctt cgcctctgcc 58860tttgccttcc tggggctctt ggcgctgaca gtcacctttt cctgcaacct ggccaccatt 58920aaggccctgg tgtcccgctg ccgggccaag gccacggcat ctcagtccag tgcccagtgg 58980ggccgcatca cgaccgagac ggccattcag cttatgggga tcatgtgcgt gctgtcggtc 59040tgctggtctc cgctcctggt aggtagttcc agggttgggg gaagtcagga tgcgtgtgcg 59100agtaagcggg aacctaagac tttgctaagt ggcagcaccc gcaggggtgt gtttgcaacc 59160gcagagcgct gaagtgtcct ttcctcctgc ccgcgcggtc ttggttacag cttcagagct 59220gagctcctca cttactagct gagcgccttc caagagccag gattcgcatt aggcactgtt 59280tatgagcaca tagagatcac agggctgctc acattctagt cgggtaggag acaggatgtg 59340tcattgaaat gacatatatg cagagctaga agtttgtcct gagtgcatta aaagcagaat 59400tgctatgata actgtagcct gatattgtgg ggtttttttt tttttttttt tttgagacgg 59460agtctcgctc tgtcacccag gctggagtac agtggcgcag cctcggctca ctgcaagctc 59520cgcctcccgg gttcacgcca ttctcctgcc tcagcctctc cgagtagctg ggactacagg 59580cgcccgccac cacgcccggc taattttttt tttttatttt tagtagagac ggggtttcac 59640cgtggtctcg atctcctgac ctcgtgatcc acccgcctcg gcctcccaaa gtgctgggat 59700tacaagcgtg agccaccgcg cccggccggg tttttttttt ttaatttcaa attcctcttt 59760cacgttaata agattttcag tctgcaaccc catacagtca cagccctcct gcagcttctg 59820aaagactgcg cgacctatgg gtgccttcaa ggttcattct tgatgaggca ggagaatgaa 59880agatacgagt tgcctcctca gcctttcgcc atggtgcttg accctctagg aagtgcccga 59940acgacagtca gtgggatccg gaagctcagc agcttcttca ggccagcatt tacttaccct 60000tgtagcactc aagcagggtt cgctaatcct gagataggag gtagaggttt ctccatgtcc 60060tagctcctca caccagagca atgcagagga gcaagggatg tttgcagagg ctctttgcct 60120aacacctgcc cttacgtgat atttttgagg tttttttgtc tgtgttactt ttagctagaa 60180atgtccgtgt gtaagtgctg tttactaatt cctgaagaat ggatgcttac ttaggccctg 60240gggtgtctgg gttaatagat gttgcagaaa gagcgatatg gtcactgtta caaaggtata 60300accaactata tgtattatac agttgtggaa aacctttgaa acacgtgaac gcagtttttt 60360ttctttccta attatggtaa aatatattac atacaattca gtattttaac cattattatg 60420tgtacagttc agtggcatta ggcattaagt acgttgagtt ttttgtgcgc catgtatact 60480tttaaatccc ttgcatttga aaatagaaat gtaatttctt taacctggtt tggtagattg 60540gagcacaaac cttggctcca aatcagtgac atgaggggac aaggagtgtt atgctgagac 60600ctaactgccc acaagtcgct gtatggtcac agttctttat tccaggagtt ggaactcagg 60660ctgatgtcat cactatggct tatcaaaacc agcaccagtt ttagatatac agtatttact 60720tttatggctg aggaatggag tgtacaggtg gccaactagg aaggaagaaa gagttctaaa 60780tagtaaacac tttactaaag tgtggtaatt aatataactt taaaggcaaa cagggtatca 60840gtttttcaca agtgtcattt gagtcccaca tctttgagtt tgctgtatca catgatttat 60900atcctaggga aaaaaaatgc agttaaatca attgaacaaa atacccatac agttaatcag 60960acttataatt tattgtttgt gcattttgtt tttaagtcaa agcgaagcca tgtagtgtgt 61020ttttgattta gacactttaa ttctagtacc aaaagtatgg tggcctcaga aagaaagtag 61080atcaatcttg aaaactgtaa tttaggatgg ccaaactcac tctttcaaga aagactgcat 61140ttgaaactag tcttacttaa tttcaattta ttttcatttg gataatactt attgatttat 61200agcattttac tgggtacttt attaattcaa ttataaaaac attaaacaaa ataaattaaa 61260catgtgatca tatcaaaaag gagaattttt tttttttttg agactctgtc tcaaaaaaaa 61320aaactccagg ctggagtgca gtggtgagat ctcagctctc tgcagcctcc acctcccagg 61380tgcaagtgat tctactgcct cagcctttgg agtcactagg attacaggcg cccgccacca 61440cacctggcta atttttgtat ttttagtaga gatggggttt caccatgttg gccaggctgg 61500tctcgaactc ctgacctgag gtgatccgcc cacctcggcc acccaaagtg ctgggattac 61560aggcatgagc caccgtgccc ggcctaggag aaatgagaac tttttaaaga aaatggttcc 61620atcggctggg cgcagtggct cacgcctgta atcccagcac tttgggaggc cgaggtgagt 61680ggatcatttg aggtcagcag ttcgagacca gcattccaac atggccaaac cccgcctcta 61740ctaaaaatac aaaaattagc ggggcatggt ggcatgcgcc tgtaatctca gttactcggg 61800aggctgatgc aggagaatta cttaggctag ggagacagag gttgctgtga gccgagatca 61860cgccactgca ctccagcacg ggtgacagag caggactccc tctaaaaaaa aaacaaaaaa 61920caaaaaaaaa actccaaata tcttcaaaaa gatttactgt acaaagttgg aattactaca 61980aagaaaagaa actggcaaag ctgctttctt aatagagaat tataagaata aaaacactag 62040aatttagtga attgaatttt cagcatatta gtgttaaaat gagagattaa cgctgttttt 62100tctaactatg agtgtaatat aggtttcctg ctagcattcg ttaagttggg tgtaggatac 62160tattctaatt gtccaaactt cttacttgaa aggaccctga gtatatcctg gaagactatc 62220atgactataa taaatattca gttgttactg caggaaaact tggtagtaca gaaacaccat 62280aataagaaaa tgtcccaaca gctacaagtt cacactacat aaatacttaa atagaggttt 62340atgtatcctt aaacaaaagc tttgtttcct ttttcttttt ctcagaaatt ttgcaaaata 62400caatataaaa tgtgaaatta agagagtaca gtgggaaatt atgtcaccat gatagatgac 62460ctacagtttt tagttactgc ttatgtaagg catcacattg tcattctgtt gcaatcagcc 62520agttcatcca gcaatgcaat ctatttgatt tttatcatta ttttatgttt tatattttat 62580gaatgatgct caccatagct cagttgtttg acctgatagc taaatttcca gaaatgggaa 62640taaccttagg attttgttca aaggaagcta tgagtacatt ttgtactttt ataacttggc 62700actcccaaac aggtagaatg tggctaatac tgtaagctat ctcaaaaaca gtaaatacaa 62760agccagctat gaacatcatg aaaagtgagg tatgagcaac aaacagtttc tcatcatcaa 62820tcttggaact ggataaaagc agccttagaa acttttgcct ttaatcttga accactgaat 62880tccctgctca gtatccacaa atacagagat gtttcagttc aagaagatat ttcacaatgt 62940agttgtgatt ttttttcatt ttagttaagg agtcactgat gtattttgac aagcgaaatt 63000tgcctttaat atgccacatg tggtagatat gtatgatgaa ttattttcag aaataatgtt 63060gttttatagc atataaaagt attattcaat tgctaatctc ttctgcctca actttctgcc 63120atattcagga tgttgcttca gagaaagtga ttaaacatat gaatccacat tgtaggaaaa 63180atattctata atttttctct tttgaaaaag gtgtgaaggt caacaaaata ttctatgatt 63240ataaaactga ttaatgttat aatctgttca gaaaagcaga atttccacat ttatgagtga 63300aatatttaaa gaattataat acaagatagg atgtaattaa tgcctttggc tctcagaaaa 63360aagtcagtct gaatttgcca gaggagattt ccaggaagat gaattggcat aacctcattt 63420catgacttca tgactgagag caagtgtgtc ccaaaaccca gcccaatgag aacaaagggt 63480gcacactaga tcaatgggaa tgatgtttta aaaatgttca agaaaacaaa tttagatggt 63540tagtccctga atgacaagat gaggacttca ctcttttgag tttatcagaa agagccaaga 63600attaaacact tcttaagcca tcatctttga agaaattcac atggtctgaa gttgtaatat 63660aattgtgagt cactggccct tctcttttga acaagctgtg aaggtcaaca actaaattat 63720aaaagtttcc ataggtttag ttttctggga ctaacactca ggaatatgca ccacaggcat 63780ctgtaacaga ctatgattca tctgagggtg cagaggggtg gtaggtgagg gatactgggt 63840gctaagtttc agtttttggt tctgcccttg cattgcagga cagaaatgca gggaagtccc 63900ccctaaaacg ctgtacatct gcgtaacatt tttgcaacct gccagtgttc cacatttatc 63960tcattcatgc ctcacaacac taagatctac aactgtaaca tgtcaaattc ttactctccc 64020attttttcca attgctttac aaaaagacag gaaggaagtt tgtatttcaa ctcctgccag 64080ctgtgcttct tgagaatcat tagacaatct ctctagtcct ccttttccct agaaacttcc 64140aactttcata aagatggtaa ccaatgaagg agcctcattt tgggggggtt cctcttatat 64200cttgtcccct aagtatatgg caacatttta ctgtaaacat agtaaagaca gtctctgacc 64260tatgcttttt caacttcatg atggtgtgaa aggaatgcac attcagtaga aactgtactt 64320ggaatttgga atggcgatct tctattgggc tagtgatatg cagtatgata ctctttggtg 64380atgcatgcac agctcccagt cagccacgca aacacaaggt aagcaaccaa tactccacag 64440tggactgtgt tgccagatga ttttgcccac ctgtaggcta atgtaagtgt tctgagcatg 64500tttaagatag gcaaggctaa gctatgatgt tcagtatgtt aggtgtatta aacgtattaa 64560acgcatcttt gatcttacaa tatattcaac ttaacaatat tttcaactga cagtgagttt 64620acccagacgt aaccctgttt taagttgagg aacatctgta attgattctt atacagaaat 64680gacagttggg cagcatatga ctgagataca tacaacatgg gaactctctg tgaattcctg 64740caccctgctc tctttacaca gagcacttct ttctccattt ttcattgcca ctttacccct 64800cagccattca gcaatatctg ccaagcaccc acctctcttg gattggctaa gatctcaagt 64860tagaagccat tttctagcga agccttcctt catcctctct aggccgtgat tgggtgccct 64920gatctcatga taccccgcat ttccctttca cagtctgagt cacacttcat tggagacatt 64980ggtttattgt ctttctgttc ttttagttct gctaaggtca agtacagtgt gtgtcttgca 65040cattattttt tcctaggaac tatcataatt gcttgccaca taaggtctgc ccaatacgtg 65100tttattgact gaatactttt ttttttcttt tgagacaaag tctcgctgtt gcctaggctg 65160gagtgcattg gcatgatctc gggctcactg caacttctgc ctcctgggtt caagtgattc 65220tcgtgcctca gcctcccgag tagctggggt tacaggcatg caccaccaca tgccctgcta 65280atttttgtat ttttttttta ctagagacgg gatttcacca tgttgggcag tttggtctca 65340aactcctgtc ctcctgtgat cctctcgcct ctgcctccca aagtgctgtg gcattacagg 65400cacgagccac cgcgctggcc ttgactgaat acttgagcaa ccaaatcaga agacagggtc 65460tggccccatg taaataagca attacttttg gccttagaca attcacttga taactttatg 65520aacccttccc ttcctttcct ttcctttccc ttcccttccc ttccctctcc tcctctctct 65580tttctttctt tttttctttc tctctttctc tttctttttt ttctttcttt ctttctttcc 65640ttctttcttt ctttcttccc tccctccctc tctttctttc tttctctctc tctctttctt 65700tctgaaacaa aaatgcctac ccagctcatt tcacagtgtt ataatgaaaa ccaacaagat 65760taacatatgt gaaaataatg gggtagtata cagtactatg aaaatacttt attactgagt 65820ccttttagca agctgggaac attccttcca tatgaggaat accactgtga tgttttactg 65880gatagtagtg ttgactcttg tgttgagata gacctttctc ctagcaaact ggagaaagga 65940ggcaagtagt gtttcacagc ttgattagtt atagttcatt ggggaaaaaa tgtggtggag 66000aatgcaactt tcagaaacag aaagaaaaat ggtaaaccaa ctgtgcctaa tttttctttc 66060tttttttctt tttttttttt tttttttttg agacagagtc tcgctctgtc acccaggctg 66120gagtgcagtg gcgcaatctc cgctcactgc aagctctgcc tcccgggttc atgccattct 66180cctgcctcag cctcccgagt agctgggact acaggcgccc accaccacac ccggctaatt 66240ttttctattt ttagtagaga cagggtttca ctgcattagc cagtatggtc tctatctcct 66300gacctcatga tccatccacc tcagcctccc aaagtgctgg gattacaggg tgcctaaatt 66360tttataaggt attgtaattc atccctcctt ccctccccct gccaccatcc ctcctctgca 66420ttctctcagc cctgtaatct gttccctttt acagcttcta gttagctctc taactccaaa 66480acacactgat cccatttccc ctttccctta cacctgaggg aagagctagt accttgtgtc 66540ctgaagacct ctgtcacagg tctggaatgc tcttttctgc tcgatggccc tgagaagggg 66600aggacttatc tgcagtgaac agttcccctc cccacctgaa cagtcattcc tctgtgttgg 66660agttaaatgt gggtcttgct agcacccaca gcctctcatg tgagctcttt ccagtcaggg 66720tcgtcttcct gttatcctat atgtgcacaa acctattctc aatggaatct tgagttgaac 66780taattgtgca tctaactgtt gtctaggatc aagtcattct gcaaacttct tgagagagtc 66840tctccctcag ttgtcttcat cttttcacag gcaattagaa atgttgggac tgaacagaac 66900ctgtgctgct gactgacagc ccagaattga atttacacaa gccttaagtc agaccaaagt 66960gtgtgaatca tagagttgta aactgcttca tacagtttga ccaggtcctg acaacacatt 67020aatattattt gtggcctgca aatagcacaa tgtgttattt ttctatccag tggcctttcc 67080agatattctc tgggaccaca tttataggga ggacatttca gtgacttatg tgggtgctta 67140cttaaaatcc aaatattatt ttagtgaact aattttatga tagatatttg aaggatcatc 67200gatctaaaat gaaaaatggt agcagaaatg gaagaggagg cttgagtatt cacactttat 67260ttttagaaaa aagaaaatgc ctactaattt aggcaaaagt tgtgcaatat agatacaaga 67320taaattctcc accagatacc attactgttt aaaaactctt ggtaatacaa aaacaaaagg 67380ccatgttgga actggaagcc tgtttatttc aagcaataat tggtccccaa atattaagtg 67440taatttaaaa catatacttt ccactaattt tttgttatat cctgagagta aatttgattt 67500gtatatcttt ttgtctctct ttctgaacac ataataaatg tcattttcta tggttgagaa 67560tatgctgctg ttgtgattca taaaaatttg gaaaaaagaa gtgtgtgatt aatgttgaaa 67620atgatgaaag agtgattgta aaagataaga ttcattctga gaaattcaca agacagtaaa 67680gtggagcact aattgctgtt tgatgtatga aatttcactg gtaacatctt gctaccaaca 67740accttatttg ggtcagagct cctggcagat cacacacttt catatttccc caaatagaag 67800aatgcctcaa cagccagctc ctcagccaca gagtagcatc agtgattaag tgacccccga 67860cttgtgagga ctcctctctg cttcataaga agaacagaag aaagttatta aagaagcaag 67920tgagtgccac agagagaaca aggcactgga tggcaagaga cctggatctg gtcacggcct 67980tgccatgtga tctaagtcac tttatcttgc cggactttcc ttttctaacc ggtaagataa 68040aacgattaac gactctaatc ctcctttcag ttctcacttc ctgtagctct aaatagatta 68100aatcgtccct attggacata ctatttcaga tatatggggg aaaatacgta aaaacttatt 68160aggaaaaagc atagaaatgt atgcaaatgg acaaaataga tcacacattc ttttatccag 68220cgattctata aatatttatt gagagcttgt tatgtgcctg ccactctcat cactctggta 68280atacagtggt ggtcagcacg gattcattta gctataaagg ttgagcagtt acgttgctcg 68340agagagtgct attcacatta tattgtaaga caatgacaat ctttggagtt gagcagtatg 68400caatctgtgc agctctacgt ggtagcctcg atggtaagcc aaatggaatg ctatcttcct 68460tcacatagct ttcagtctac taaggtagac agacagtaga taatcagtcg ttccaataag 68520ccaatgtaac tagtcggata ggtcttactg tggttgccct tactatcaca catgagattg 68580gcctcatgtg ggcaggacac ggctggtcca gtgcagcatg atggccctaa tggaacacgc 68640ttggccccaa ctgtactggg tctgtgcttg gactccataa ttagggagca cattaattca 68700cacctgtgac tccagctgta atgctcagct gcaattctcc gagcacaaac actgccagag 68760tgagtacatg gactcaaagg gctgctgacc tcaggccaca tgctgctcta gaatcagcct 68820tacttctggg atcattaggc tctgggtggt actcagatta tctcctgaga aatgtgtctt 68880tccctttctt tttttttttt gagacaaagt ctcgttctgt cacccaggct ggagtacagt 68940agcacgatct cggctcactg caagctccgc ctcctgggtt catgccattc tcctgcctca 69000gcctccccaa tagctgggac tacaggagcc caccaccaca cccggctagt ttttgtattt 69060ttggtagaga cggggtttca ccgtgttagc caggatggtc tcgatctcct gaccttgtga 69120tccgcccacc tcggcctccc aaagggctgg gattacaggt gtgagccact gtgccgggcc 69180cctttctttt attttttatt tttaattaat ttattttatt ttgagatagg gtcttgctct 69240gtcacccagg ctatagtaca gtggtgtgat catagctcac tgcagccttg aactattggg 69300ctcaagtgat cctccagcct cagcctctgc atagttgtac tattagttgt gcaccaccat 69360acccagtttt tccctttctc taagttttct tttaaggctt gtatgtcttt tccctgtagt 69420gaatgtggcc aaatccactc tgctccaagt tttttggctt caaatcttga gagaagaggt 69480tcagtttctt gatctcctct ttgaccagaa gtccctgggc cccttaagca ccatctacct 69540cttcccaagt gaccacagag tgttgtgtgg gttttccttt ggtataccac acaatttccc 69600ctgtgtaaag ctaatgattc caaatttacc agaataagta attcttctga aacttctgct 69660ccaatgtaat ttgttctgcc cacttgacca gagaagtgca gatggatgct ggcatacctt 69720tttatacatc aaaaacgagc actttatgaa ggcaagagaa aggtaatttg agagagtgtc 69780aaggaagatc tcctactgat gcattgggat tagggagggc atctctggga aaaaggtcat 69840ttatgacgag tcttacaaga taagacctca aggaagaaag agcagtatgt gaaggtgtca 69900gtgcaggaat acactggaag cattggagga actgaaagaa ggccagatgg atggattaga 69960gtgagtaaaa aagagattgc ctgcaaggcc agctagggag ggaggcaggg ccagataatt 70020cacaaccttg tcatttttgt ttgggagttt agatttctac cacaagtaca atgaaaaaca 70080actgaaagct gtaggggaat gatttgatct gcttcatgtt ttaagaccac ttaaggttgc 70140ctaatggatt agatgggtag agcatcagag ccctcgagta ctgaggacag atggagatgt 70200agagacatta atataaagaa aagccttctt ccttaaatgt tgctttaaaa gaggagtggg 70260aagtagcttg gcctagaatc ggtgagagag gggaagcagc agtggatctg tgcagatgca 70320ccacggaaaa tattgaacca gatgtgctgg gaatggtaag cagatctgtt tttgtagaag 70380tacatttgag aatgtcaaaa actatagata tagtcaaata gagactgaag ggaaagggag 70440aaacacataa gtagattgct taagattagg aatgagaatt tgtatttcat tttgggcaat 70500ttgtattgat tttctatact gttctatatt tgtaaaataa gagagtgtgc attataaaac 70560ctgaattcac aaacctcgtt taggaaaaca actcatctaa tatttgatat ctttctagaa 70620tattcttagt gaaattgttc cagctttgtt tacactttgt tgaacaccct taataatagg 70680aaatttttta cctttcaaga actctaggaa ttttcttaga gccaagattg tgccaggctc 70740tttctcacac atactttttt taaaatttct tgcaataacc ctatgagata ggcatttttg 70800tcgccaaagt tattatgatg agaaaactaa agctgagagg agttgagtga tttgttcaag 70860gtagcaaagc tattaagtag acaggctggg attgaaaggc caataaattt tcaataaaga 70920aacaaagctg agaaccattt agaattcatt tgtccatgtt tcctccatgc catgatgcct 70980tagaataccc caattcatct tgagctgttc tttgtattag aaaaattcat ttcccttata 71040ttgactgagt cagaatctgt cctccctttt gcttccaccc ttcggtgcta atactagctc 71100ttggattagt tagagcaata ctacatttat ttttatttta ctattatgtt ttatcacaaa 71160gaggttacat tttttactga caactcagtc actgcataca tactttattg cacaagttat 71220aagtggatca acaattatat tagcaatgta aactcatgtg catttacata aaatgactgc 71280cctagattct ggtgcatttt gtgccaggta ctttggtaaa tatggcaaac ataaagggac 71340ttagctattt caatttatgt gaacaaaagg tagcatatat atcatcttat aaaatacctg 71400ttatgtcaaa tccaggaaaa tcaataaatg acaaaaggaa cacattaaaa gaaaatttag 71460cgataaaaat gttagattaa aatattaggt ttaaaaactt taacagtttt tccctgctag 71520tataaatcat tgttttgtag ttattaatat aactcacata aatgagtttt aaaataagat 71580tcttctcact caaaataaaa taagataaaa ctaatattac ctccaccaaa ttacaaagaa 71640atcctatcaa aatgctggcc agataaagaa tccagataca gacacatcat aacaatacta 71700aatttactta aagacatctt ttatttttaa aggttttttt aatttttaaa gataaaaata 71760ctataccaat aactatacag agtagtatta ctaatgcagc attatattat taggtaatga 71820ggagttctac taagttttta agcagaaaaa taacaaaaaa ttaccttgac cacggcagaa 71880ggaggagcca tgatttataa tttatttagc agacacttat tgaatactga ttgtgtgtaa 71940ggtagtgcta caatgacgtg acccttgtcc tcaaggaatg tgttgtcttt aagacgattt 72000cctggagaaa cttgaaattg actcaaaaaa aaaagcagca gcattttcca tctttaattt 72060tcaaaaaaag aaacaaagct gagaaccatt cagaattcat ttatccatgc atgcaatcag 72120tatttgttta ttgagtccat acaatgtgtc aggcacagtt ctaggtgctg gaaatatggc 72180agtgaattag gctagtttca tgctctcagg gaattagcat tctagtggaa ggagagaggc 72240aataaataaa ctagaagtct agtgattgtg ataaatacca ttaagaaaat gaagcagtgt 72300gccagaataa agcctgaagc atccttctgg cagctatgtg gagctggatt gcagaaaggt 72360atgtgtggga acagggacac caggtagaag gctgttgaag tcatccaggt aagaggcggt 72420gacaactggg attggagtga agtacccata ttgacatagt gttttggaga cagtttgcca 72480gacattttga aatttctcta atccctgtcc tatgactctg acaagaaata ggacctctct 72540cttcctctcc aggcctttgg ttttgaggtg gccagctatc tacatatggc tttccatttg 72600gctccccaag aaggaagtga ctgctctaag cttacacaac taggaagaga gaattaggag 72660gtaaatttga tctaatagat gttatttcct tttttctgag acatgaatca gaacagtcac 72720acagagacac agaagttacc aaaatttgct tataaaataa tagaatgtca taagattcgt 72780gatccccaga agatctggag catgctgatt tccaggctag gtctcagaag aaactttttg 72840ttgtcattgt tctttctaaa cgggttctgg ttactgttgg cctctttttc tcctaaaagg 72900agagcagttc tcaaattaat actccctact tttaaaatca ggattgtttg gctgcaaatg 72960atcgaaaact aatgcaaact agcttaagca aataaataaa tataaaaagg tagggggagt 73020tgcttgattc acataaattg caggagtgtg aagagtggag gtggccttat agaagatgga 73080agccagcact gtctctttct gagactctaa cttcagcttc ttgctatgta tcagaatccc 73140cacttcaggc tctgctccta aggaaccagc ctaagccata gctttgcagc tctttggcta 73200ggtcttgtgt tcttttttca aagcttcaag ttaataagtt tatatttgtg gtctacatgt 73260gaggttttaa ttttgctttt ataataatac aatattttac tgaatacatc atcttgaagc 73320actgtttaga catcatagat tttttctatt gttttgtttt ataaacacaa gaaacagtca 73380tagatgacaa agttgcattt tcaaagacta gtatgagttt tctaggctat gaggaaagat 73440tcaggctcag agaattttcc agattcaggc tcagagaatt ttccagaagt aagagtataa 73500tgagaggtgg ccttataaga cgaaaatctc acgaatactc cattaacacc atagtgagat 73560gactagagaa gaaaaaagaa gttctttctt gatttttcca taaaggatga gttgtagtct 73620gggttttatg ttattctggc aaccagcttt ttctttataa atcagtagct gtgctaacgg 73680ccaacatgct gggtggcaga cttttgaatt tatttaatgg tagtaatttt ctccaagaat 73740agactaggga tttgcctgga tccaaaagca aatttcttaa tattagcttt tgctctataa 73800aagacattag ggaacatttt aaaattcttt tctctagcat ctatttctaa ttaaattttg 73860ctacagatca atatcaggaa gcatggaata tcttctgact tcagaattct accctcctcc 73920ttagacatgg ttctgggttg caaacaacca actgtgactc caagtgcaaa gagcatatgt 73980agaaggattt caggagctca cagagtccag agaaatctgg caggtcaggt ttggaaaaca 74040ggcaagaccc aggatgttct tgatgatgaa cttcaggctg cacagccaaa gtcctgctgg 74100aaaaatgttt gctgcatgcc tctgtgatga atccaatgtc taaaaacccc cttcttcttt 74160gtgttatgct tgtaaaattc aaacttctgt tgccagcctg actgctgtag cttgggttat 74220ttacccctat gctataacaa aggatgaagg ctcaggcacc tccagcactt ggtttatgac 74280atatgtgctg ggatttctct cccacccaga atacagagaa acggttatcc agaattggga 74340tattaataat aaaaggtggg ggttcatttt taccatctat ccaatttatt cgtagaaaca 74400ttataatagt aacatacttt tgaagccttg tgcacatgtg cgtgtgtgca cgtttgtgtg 74460tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgttttcctg ccacctatct gttgggaaat 74520tggggtagca gtgagactgg tggagcgcaa actgaagaga gccacaaaaa ataaaaggac 74580ttctatttac tgaatgcctg ttatgtgcag gtacatactt gacaattacc ctttccatat 74640attacctaat ttaatcttca caacaatttt tgagtttgtt acctttcatt cccatgtttc 74700agttgcaaat aataagacag aaaagatttt ataactttct ctaatcatat tcctagtaat 74760tagtagatct taggcttttg tacactataa tgctatgtgt cacgtttttc tctcttatta 74820gtttataaaa atattttcaa atcacttcgt tgaaaatatt gtacaaaaaa gaacaaattg 74880attgagaata gtaaaaaaaa aatcactgaa attgtctggg tcagataatt atggtgatgg 74940agatttcctg tagaatagct attgattcat caactgattg acctttgtag gagcttgagt 75000atctagggag catattctga aaaatcccgt agaaggtgac aggagttttc acttgtctga 75060cagtaacttg ttaaatgatt ccattcacca cgataccggc aatttaggaa gtgttttttg 75120taaaggtgtg gcatcaattt tatttacttc tgtgtgtctt ttaggcttag aacattcaca 75180tccagtgata aagttttact aggcaaacat ttttcagcta gtacaagtca catatattat 75240tcttttcact aaactattta taaaacaaat aaaactgtat attgaattct ttttgcttgg 75300tgggaaagtg tttgactaca gtcaaaatta gaaactttgt tttgacagtt ctaaagcctc 75360cagtatgata cagtgcaatt gggattcttg caaacctgct ttcatctttt ttcttataat 75420tagggaaaag agaaagagcc ttagagaacc tcagtgtgac cacggtgact ctctaacatg 75480gaattcaggc atgttactat agtagcctct cacccagatt aaaaggcttc agattcctta 75540gtttaatcct caagatcttg gatttggcca catttaccac tgtaccattt ttttccatcc 75600ctaccccagc atccttacct tcagtgataa aaatcagttt gcagctctta taatgattca 75660cacaatttca cactcagaaa tgtggacttt gtctcaaata cctacaaagc atctgctgtt 75720ttggcagaca ggggtttctc tgaacctaga aacttattta tttatttact tattttgtta 75780ttattattat tattattatt attattatta tttgagatgg agtctcactc tgtcacccag 75840gctggagtgc agtagcgtga tctcggctca gtgcaacctc cacctcccag gttcaagtga 75900ttctcctgcc tcagcctccg gagcaactgg gattacaggt gtgcaccaac acacccagct 75960aatttttgta tgtttagtac agatggggtt tcaccatgtt ggccaggcta gtctcgaatt 76020cctgacctca agtgatcctc ctgcctaggc ctgccaaagt actgggatta caggtgtgag 76080ccatcacgcc cggccataga aactgattta agtgcccctc ctctttactc catagtgcct 76140gtgcatacct ttatacttaa ttatgcacag ttataattat ggcaatgagg atgattggcc 76200acaatagaca aatgcttatt ttataattta tgcacattgc atttcacaga tataatttca 76260ttttgttcta ttgatgacaa cttgagggga tactgtcatc ttcgttttca gaagaggaaa 76320ctggttttga gatgtaaagt aactttcttg gggtcaaatc cagatttgtc tgattttatt 76380gcctatgtaa ttaaccacta agctttatgg tctccctcta tttgtctgat tcagtttaaa 76440ttataaactg cttaaaagca gagatcttgc cttgtttttc aataaatctt cctggcccaa 76500agaaatcacg caataaatga ttgtgaaatg aatgaagaag ggaagtgtaa ttaagtgaaa 76560aggacactag aataggaata agaagatcaa ggtataaatc ctagctttaa gagttttgca 76620tacattagat ggttttttaa tcttcacata aatcttgaaa tgtaggtgac atcagttcct 76680gttttttgtt ttattttatt ttactttttc acttgttgag taagcaactt gctttaaggc 76740cacagatgat aaatagtgtt gagtgaatca aactcaaatc tttttcattc taaagccact 76800aatccaactt gaatctaatg aatcatgggt attattaata cagtaaaagt tgttagtatt 76860taagataata gtgcatattt ttctttattc tttactttta ttgtctccac tttgttgtta 76920gtaataaaaa ttctagagaa gatttaaaca tacctttagg gtagacaact gtaagagaga 76980caacattgtt tttataatgg aagagagaag ttaattggaa gcaatgggaa ggagcacaag 77040aaaacagttg ctctaatgcc tggtgtttaa agacggaaaa aatgaaaggg atgtttggaa 77100aggtgccctg gggataacaa ttaaacatga ggaatttcgg cgacaatagg gaaacatagt 77160aaaaaataaa aaacaaagga aaaaacataa tgcttcaaaa gttgctccag agtgtcacta 77220tatttatgtt gccctcagtc attattagag atgatgtaat gtagtggttt gggaataaaa 77280aacataactg aaatccaaaa ttagggcagc cttcagagtt ggttaatccg aaagctcagc 77340atcgtcatct aggaccaaag tttgccgcat ctctctttcg cagtacagtc ttggcttcat 77400cctacggctt gttccccgca agtggtagct gtagtacgtg cttcctggtt aacttctagc 77460aggagagaga agctgtgttt ttctatgact ttctcttaaa aacgaggaaa ctgttggatc 77520tcattagcta gtatggggtc acatgatcat ccctaacccc ggcacattcc tgtagctagg 77580gatggggtca gccctgaagc acataggatc cagtgaaaat aactatgtcc atgagcaggg 77640gagatggatg aatggaccct gggtaggtga ccaatagtgg acactgcagt gatgatacct 77700acacagttta attgacagaa taaaggtgga aaaccctcaa aaagaataaa gcactaaact 77760ttcatctata cagcatagta gcatagtagc cactagccac atatggttat ttgcctcttg 77820aaatgtgatt aatccaaatt gagatacttt aaatgtaaac tacacagcag agttttaaga 77880ttaaatacaa aaaggtaaaa agtaaaatat tgcattaata atttttatgt tcactacatg 77940ctgatgtaac atttcagata tattgggtga attaaaattc aaaatgtttc tttttacttt 78000tttaaaaaaa atctggctac tagaaaatgc aaaagcatac atgtgctgca gatttgtggc 78060ttgaattaca tttctcttgg atagtactgc attagactga tgattttcca attgttttaa 78120ccggggggac tatgtcaata catctcttta gagaaataga tgaaaacaga cctgttctgg 78180ttaaatgggt tggggaaccg gatagttcca agtctcaata gatctatttg ccaattcctg 78240caaaaccatt cctgaaaata tctacttcgc cttggtgagg ttgcataaaa tcttttgttt 78300ttaatttcag tgctttgaga ggccaaagca ggagaatcac ttgaggctag gagttcaaga 78360ctagcctgag caatgtaggg agatgcctgt ctctacaaaa atttaaaaag aaaaaaatta 78420acccggcatg gtggcacatg cctgtagtac tagctactag ggaggaaccc ttgaacccag 78480gaatttgagg ctacagtgag taaattatga tggcattact gcactccagc ctcggtgaca 78540gagaaacccc gtctcttaaa aaaaataaca aaagaaactt acatcaaata taggatttta 78600gatgaattca ccaaaagcat tttccctatg atagttgtag gaactgtttt attattactt 78660gtttaaggtc ctccctagaa ggtaagtagt acagtaagaa ttgctctcac tgggttcaca 78720cgttaaggag agagggagtc aatggtgttg ggatctgttt atctttttac aggagcctgg 78780gagcctgtga tcctcatagg agttaggcat ttgagtcatg gtaggaacag gtttcttcct 78840catgtttgct tccttcttgt tcttattatt tactttgctg ttggaccaac ccagaataca 78900gagaaaaagt tatccaaaat tgggatatta ataataaaag atgggggtcc atttttaccg 78960tctatctaat ttattcttag aaacattata atagtaacat atttttgaag ccatgtccaa 79020cagccatgtc catgaccaaa gtcccacagc aaagtaaata ataatatagt attaatctaa 79080ataatataat attagtaata atatagtatt aatccaaata aatcaataga agagacaaaa 79140gcagcctaag ggggtaaaca cggaagatga acacattata attttccatt ttcctgcatt 79200gcatacaagt tgtgtcttat tcaaacttcc ttcaaacctg gtataaaatt gtaagtacta 79260accattattt aagtgagttt ttaaagtccg atttcctgaa aaacaaaaga aacatttttt 79320tcagaagttt cggaaactaa agtttacaat gagtaatata aatatcgcat ggcacttgct 79380ggttttcagc tgccacctat gtgttgcttc cgtttgtaat acataacatt caaataataa 79440caaaacagca agtagtactt acatagcact tatataggtc agggactgtg agtattactc 79500agttcctatt tacagtcaac aggacaagag ttagctgtaa agaaagctgg taaatttagc 79560caggggtgtg ttttgtgttg ggaaaaggaa aatgaatgaa tataatgtta ggaagttcct 79620gatgccaata ctgagtacaa gcctaacctg gggagggaat tttgcaagtg ctaaattaaa 79680ctgttactga tgtgttatct cagattctgg ggggtatttt aaatatttat tacagtataa 79740gcaagggggg agtaatcaca caatgccaga ttcacaactt gaggagctga aagtatcaca 79800caatagcatt gctttcgctt taacattaag aacagtctgt gatttcctaa tcatgacagg 79860aagtttggcc ttcaatgcct caacctttcc aaagagagcc atgttaactg ggtttaaccc 79920acaattttac tgcttggctc ttgtgaaggg aaaaagttgc aaccaaatat ttctggtgac 79980tttctctcct cttggggcta cccagtagca gcattgaaga cattcctgca actcactcac 80040ctcacaggca agcatagtct cttatagtaa gtattatata agcattagtt gctgttttgt 80100tattattcgt cttcagttgt taagcataag atatggtgtt atatttttgg gcaaaacttc 80160tataagcaag gagtgctaga atcatatata acacttacag taataaaagg acttttgaca 80220ctaagtatta gaaatttgat gatatcctca ttaaagactg tgttgatatg cataatatct 80280tctttttttc cttgggctat attttaaata aatgttatta gatatacagt taatagaata 80340tcctcttata gattcaatat attttatttt aaatacttgg ttctcctttc attctataac 80400cccaacttac cagcctcagg gttactactg gctgtcatct gaacaccaga acgttccact 80460taaaatcatg aatgataaga agatgtgctt atagtccatt agcacctagt ttgaagctgt 80520tatcctggct aattataata agaaggaagg aaggaaggaa aaaaaggaag gaaagaagga 80580aggaaggaag gaaggaagga aggaagggcc tggaatcttg aaaaggattg aatgatgttc 80640cccttcctgt ggccatgtgt tctcattgtg gggtggggga ggggggaggg atagcattag 80700gagatatgcc taatgttaaa tgacgagtta atgggtgcag cacaccaaca tggcacatgt 80760atacacgtaa caaacctgca cgttgtgcat atgcacccta aaacttaaag tataataaaa 80820ataaataaat aaataaataa ataaataaat aaataaataa ataaaagaat caagtatttt 80880tttcagccgg gcacggtggc tcatgactgg aatcccagca cactgggagg ttgaggcagg 80940tggatcacct gagatcagta gttcaagacc agcctggtca acacggtgaa accccatctc 81000tactaaaaat acaaaaatta gctatgcacg gtggcgggca cctgtaatcc cagctactcg 81060agaggctgag gcaaaaaaaa aaaaaaaaaa aaagaaagaa aaggatagaa tgtcggtaga 81120tttgtccatt acatcaaaac tctagaatct gtacccccaa atgggcagga tgcactatac 81180ccttgctctc attcacttat ttgttaattc aaaaacttta ttaatctact gggcttggcc 81240cttgctgtga tatacctaga atataggata gaattgatcc ctactcttgt aacaattttc 81300gattattgag agagaccaac aattaaaaac aagcagcaag ataaaatgta gagaagtaga 81360gaattatggg aatccatatg aagattgtat taaggcttgc ttttgtcaag agaaatatca 81420aataaattga tgtttcaaga ccacgcacga aataagtcag aatttttcaa gtaaagaaaa 81480agaggatgag agttttaaga cttaaagatc cacatttgta atgcctcaaa agcaagaaag 81540aactgtcact tttaaagacc tgcaagaagt tcaagataat tggaatactg atgcatgagg 81600gtaaaaaatg gtgagaactt aggctagaga acacaacagg tactcaccaa gtaaagagga 81660gctttgaatt taagtgtttg gactttatac tcagggcaat gtgtataaat gaaggacttt 81720gcaaagtgga ataacaagat taaaatttca ttttacaagc atctttttta agcagcagca 81780tgggccaggc cagatggagg ccaacaaatc atgtcattga tgattgtgat agttcagaca 81840agagacgatg gagacctcag tttatggtca attgggggtg taaatgagag aagtgataga 81900ttaaaaaaaa aaccataaga ggttagttgg agactccgtg attgattgga tgtgaggaat 81960atggtgaatg tggtggaatg tggagagaga cagagagacg tagagaaaga cacacagaga 82020aatgaatcaa gggtaactcc caggtttctg ttttagagat ggggatttac tgtgataggg 82080aagattaatg tagaaacacc tttgaggaaa aagataagaa ttttcatttt gaaaatgttg 82140aatttgatgt aagacattca aacaaagccg tgcaatagca gctaaactaa tccatttgga 82200gctcagagaa aaggtatggc ctatagcaca taaatggggt ttgaagccat tgagatgaaa 82260gaccacacat agtcagttta ttcacagtaa gaagaggagg ctctctaaga tacaattcag 82320aaatgctaac atattttgag acagcgagaa acaggagcat ataaagggga ctgcagagga 82380atacccagaa aggtagcagg aaaattagta ataaaatcag aacaaatggt gactcgaata 82440ttagggaaga gaatgtttca agagacagct acattcaatt aggtctgatg ttacagacaa 82500gtaagactgt ggcgttgatt ctgcaacctt gttggtggcc ttgggaacag ccttgtctga 82560ggatttgtga acacagaggt caggctgcag caggttttgg aataagttta aaggtaagga 82620gggtagacac ctcttttggg aaatttggct gtaacttctg gagcagtcta tatgtaggta 82680ggtgagaatt ccacacaggt tttattttct caaggtggca aaaatttgga catgttaaaa 82740tgtgactggg aaactgccaa aagtgggaga agttaactat aaaagaggac aggaacaatt 82800gagagtatat ggtctctgaa attcaggatg gataagatcc aggggctaga cagagggtgt 82860gccttagata gacaggacac ctcttccagt caaataagag gaaggaaaga atggatggct 82920gcagatgctg aagttggtgg caagaaggtg ggaagccccc taaacagatt ctgttcattt 82980gtgtgtgtat gtgtgtgtgt gtgcctgtgt atatgtgtgt ttgtgtgtgt gtgtaagaga 83040gagagagagc actgactaga gaatgaatta ggattggtga gcatacattt aaggacacct 83100caatctgctc atattcaccc agtgtccatc ctgaatataa gatgcatcta ttttggattc 83160tcctgtgaga tgaagtctta atagatttaa aaggcagtgt cattttgaca tactgcaaag 83220tttaataaga atgtatcata tcagattaga accatattca tcaccctgca ttgtgtaccc 83280actatgtcac aggaagggat tcttttgtat gtcatgtctg tattgtccat caagtccatc 83340actgaggcca cttaatttat cactaaattt agtaagccat aattaatttg ttgatcaagc 83400taaatgtttt gaacatacag agtgtcagac tgcttacctc ttgttaaatt atgcacacat 83460tgacctctta agaattcatc ctaaaatgac cacattatag ttctaggatt gcaacactaa 83520cactttacca atacatggat agatgattgt ttatagctaa gtcaccagct actctaaatg 83580taatgctttt taaagtgtaa aaaatagcat gtgtattgta aagaatttgg ggaaaacata 83640cagagaagaa aatagatatt gccataattc tcacccagag aaaactactt ctagcatatt 83700ttactataaa taagcacagt tattttttaa aatttggatt atactgtgca acatttattt 83760tcacttttca cattctgaac atttcttcat gttactaaat attcttcatg ttactaatat 83820tcaacagcat gattttaaat gtcagcatta gaatagatat aaattatgca tatgaaggat 83880ggtccatagt ttaagcagtg ctctattgtc agtcatctag gtttttctaa ctttctattt 83940ttatattgag gtgactatct ctgcacacac aattaattca catctctgat ttttttcctt 84000taagtgttta gttgttttta attgtcacga acactccgat tttctgaatg gatgagagta 84060aaattgaaca tttggggttt aaatgaaact atattttggg aggaaaaaac tctgaaatct 84120aagcattttt aaggattaat gtactttaag ccttgacaat gacatattat ctagactttc 84180ttttgacatc tgaaatatag gtgtttgaaa tatagtaaag ataactcttc taacttgtgt 84240gtcacccagc taatccatca tccataatcc aagaaatgaa tcctttgata gaatagattt 84300tgccttcccc ctttcagcca gaatttgaat acttacttag atattgacat tgtgtgactg 84360ttctaataga aagataaaca tttatctaga catacgtctt gagctattct gagccaagtc 84420atacagaatt tacaatttat actacttgaa agggaaattt ttatgtagca aataatgtgg 84480tagcgggctg ctggcttctt tataattttg attttccata tccagtaact gaataatgat 84540tctgcattac taaacatttt taaagtgcaa atttgaggtg agttttctaa aactacttta 84600ttagcaagaa attcaagcaa tatagacaca attcatttat ttctttgtcg tattccccta 84660agcatttttt tgttctgttt gtgaatgtca agacattttt ttttggaagc atctgcaata 84720gtgtgagtgc ctgaggcatg ggaagaggta tgctatataa attgaaggta ttaattattc 84780ttatcacatt ttaatttttg caactgctct gctagttgga gacgatacac agtaagtgtg 84840tgtgaatcat gttttagaga aggaagaagt taagaaccaa aattaggtaa ctagtctcca 84900aactagaagc agaattgagt cagacttatg gaagttcttt aactcttggt cagtatgttt 84960gccagaatct gctatttctt ttaataaaaa tacccccacc ccactacaca cacacacaca 85020cacacacaca cacacacaca ctctgctaac atacaccaaa gcttcatgaa tctagtccct 85080tgttctcata cactgtcttc taactttgta atttttctct ttcctccatt tgccccttgg 85140agtttttttt tgaagaggaa ttttgacttt tgtttcacac ctatttcctg taggttttaa 85200ttatttgcca aatgaaatcc tttcattttc cagctttgcc ccaaaaatca tcaaaaaaaa 85260actgtagagg tgtatgcaga ggccatccag tccagcccca ctgcttacta ataaatttaa 85320taaagttcag agataaatga cttttctaaa gttgtaaatg atggcaaaac tagcaatata 85380atttacttga ttcttaatcc attgttgttt taaaaaatct tataaaatta cgtagtagta 85440atgacgaaat ggcacaaatt ctactaattt ttttaatgtt tatgagctgt ttatttgtaa 85500tgttttcagt ttaaataagg cagggaaagc ttttgaaaca gtagtctagg atccagagat 85560gatttgacaa ctggagaact gttgtaacca gattggagag actacaattg tttcttaatc 85620cacagtgaag cttcttccca tgcaaatcct tcaaattaca taattaacta gtttaaaggg 85680cccagactgg ttttagtgta ttatgatgaa atacacttat caaattatag gggggaattt 85740ccagtagagc aggttattct cccataacct aacaacagag aattacggaa aacaaaacat 85800cgcagataaa aattggcttg tgaggaaact tctcttaaac acttgcatta ggaataaact 85860ggaaatgcag aaacacccca gaattgagcc taagggcagc taaccaattt atttcctccc 85920ccataaatta atttttctgg gtttcctcat ttatcatctt taatagatat gtgtggccta 85980caacccaagg catttttaaa ttaagaagtg aagtactaaa gatttttaag tgtgtgatta 86040taaatcctgc tgcaatagaa taaaatccaa ggatagaata ttaaagacag cctccctgat 86100taatgctttg aacacttaaa agaacgttat cactggggaa agggtaaatc atttttttcc 86160tttaggatgg aacaaggtaa ttgtacttga cagtagacag ggatggcgaa tttagaggtc 86220actgatgtag ttgtgttccc agttatctca agtgcataaa gaagctctct attactgctg 86280ataaccaagg gagagctctg ctagagggcc tgggagagtc acgtggaacc cagggaaggg 86340gctgacagaa cagctgcagc tccgttcagc tagaattggt ctcacgactc ccttgaccag 86400atcatttgtt atatgaggct gtttgaaagg agaaatagtg cagggtatat gtgtgtttgc 86460taaaaccagt aaacagcctc tctgttagct gagcatcctg ctttttcttt ggtccctctc 86520ccttgtatga cacagaattg gactaatagg aataattaat tctaccatat tcatgcctaa 86580ggttttctct caaagtctaa acagtctgaa agatatttgc aaacacatac acagaaatta 86640taattatggc ttaaatataa agactcaaca gatggatgct aatttgaatt tatgttcaaa 86700attggatgaa gaatttggag ccccagtttc atctctgctg ccatttggaa atggcagtag 86760catgctcact gcttctaatt ccaattatct ctatattgca catctcatga tttctgggga 86820aagctaatgt ctctcctcat gtgccagcta ctggctgatt ttataatttc agaatcattg 86880cattctcaat aggaaattac agtgtactaa acaatgtaag gcatatgaaa cctatatttg 86940cctcataaca ataattttaa attattagcc tcacaaaaat taattttcaa ataatcaagg 87000aggactgtgt aaaatgactg acgtatcatt ttaataagta attttgtgaa ttttcctgat 87060cacaaaaagt tagagaaatc caattcttta ttttatagag aaaataaaaa cataatttaa 87120gtgagttttt agataattct agacgaggcc cagaacactt tctggaggat agtaagtggt 87180caaatatatt taactattgg gtggggtaaa ggactgaaaa agataagcaa aacttttcta 87240atagtttata aaaagagggg aaagggatta ataagctttt tttcataaag ccaactgagt 87300tgaaaatatt aattttataa cttttagtca aacacattga ctgatgccac tagaataaca 87360ggcattcggt tttgcatgtt gtaaatttag aaattgagat aattgggaca ggagttagaa 87420tgaagtttac ttttacttta ttcatttatt tttaagtttc ctaggaaagt aaaaaaattt 87480aaagaagctt cctaggctat tttatctatg agaatgaaaa aatcgttttc aagctcattc 87540cttctattcc tcttaacatt ggttgagagc tcagtgtgtg ggcatatttg ttttctactg 87600ctgctgtagc aaattattgt aatcttggtg gctgtattag tccacctgag acttgctgta 87660aagaactacc tggggccagg tggtggtgac tcacgcctgt aatcccagca ctttggaagg 87720tcgagacggg tggattccct gagcccagga gtttgagacc agcctggcca atatggtgaa 87780accccatctc tactaaaaat acataaatta gccaggcctg gtggcacgtg cctgtgatcc 87840cagctacttg ggagtctgag gcacaagaat ggcttgaaca caggaagtga aggttgcagt 87900gagccaagat tgcaccactg cattccagcc tggggaacag agcaagactg tctcaaaagc 87960acaggttctg ctggctgtac aggaagcatg gctggggagg cctcaagaaa cttgtgatca 88020tggtggaagg tgaaggggaa gcagacacat cttcacatgg tggagcagga aagagagcag 88080agaggaaagt gttacacact tttaaacaag catatctcat gagaagtcac tcactattat 88140aagaacagca agggggaagt ctgcccccat gatccagtca cctcccacca agccccttct 88200ccaacactga ggattagaat ttgacatgag atttgggcgg ggacacagag ctagaccata 88260tcagtggctt aagagcaaca cacatttctc tgttctgtag gttagaagtc tgatggatct 88320cactgggcta aagtaaaggt gctacagggc tgcattactt tttaagtctc tgagagaaaa 88380gtgtttcttt gcctttttca gtttttagag gaggtcatcc tcattcctta cctcctggat 88440cccttcctcc accttcaaag ccagagatac tgtgtatctc tgaccattct tctatagtca 88500tctctccctc tggccacagc tgggaaaagt tctctgattt tttaggacac ttgtttttac 88560attgggtcca cgcaggtaat ctaggattat ctcctcacgt ctgggtcctt taccttaatc 88620acatctgcag agtccttttt gtcttgtgtg gtaacgtatt cacagattcc agggaccgag 88680aaatggatat ctttgtgtgt ggcaggaggg gcattattct gccttctatg atggggggta 88740gcattcagct cataggatga taagccagcc agcatcagaa tatattgatg taaatatatt 88800tattttaagt aaattcggtg tataattgtc catatttacc aaagaaggga ataataacca 88860tctgctttct ttaaataaaa gactttcatt aacatcattt gaaatgtttt tatatacata 88920caattttttt ttttttgagc ccaagtctca ctctgtcatc caggctggag tgcagtggtg 88980ctatcttggc tcactgcaac atctgcctcc taggttcaag tgattctcct gcttcagcct 89040cccaagtagc taggattaca ggcacacacc atcatgcccg gctaattttt gtgtttttaa 89100tagagacagg gtttcaccat gttgcccagg ctggtctcga actgctgggt tcaggggatc 89160catctgcctc gacctcccaa agtgctggga ttacaggcat gagccaccac acctggcctg 89220tgtacataca attttaaaat taaattatat tgtttaaaag taaattattg ttaaatagca 89280gtaattccat tgccttccac aagtctgcct attttaaata atataacaac aaaactttat 89340atataaagta tcttatattc tgttttttca cttaatactt atatatacta tttgtgaatt 89400attgttttat taagctttac acctgatgtt agagatgtat gtgtctgcaa tttcttgctg 89460gcatatataa tgctcctctg aacatttatt tattgtatag ctactatttg cttttattgg 89520ataaatcatt tggaaaaatt cctgtgaaag aaattacaca agaggattgc ttgaagccag 89580aagtttgaga tcagcctagt taacatagtg agaccccgtc actacaaaaa ataaaaatat 89640taattgagtg tggtggcata tacctgtaat cccagcatct cgggaggctg aggcaggagg 89700atcatttgag cccaggagtt caggactaca gtgagctatg attgtgccac tgcacccaag 89760ccttggtgac ggagtgagac actgtctcaa aaaacaaaat atgagtcaag ccccagagca 89820tgagtttgcc ttataagcca aaactggaaa ttcccatccc ctttgccagg gattcgttta 89880agaataaaaa ctgtgactaa ataagacaaa agggaaaatg gctggatacc agtgtcacac 89940agtcttgtaa gaattttcgt gtattatttc ctctctctct tgtctgtctg cttaacctgc 90000tctacctgct tttttgttat ataattgtat ttttgaaata aaattgagta tacatgagca 90060gaattggcaa taccagaaat cctatgactc agctctcttt tcctgttgac ttatcaacac 90120tgggaccagt cattatcagc ttagtggaaa aagacacaga agatggggaa ttaaaagaat 90180caaatattta cttacttcaa actaatatag acaattacat cttttatctc acaatagtag 90240tcaattttgc caacaagaat agaaaatact caaacctgat ggttaagtat cctttttccc 90300aaagcaaatc gatttcagat atgtcgatac tttgttcaat tgcttgcagt caagttcagt 90360aaccttggag ctgggttcct acgtgttgat gttatgtcca ctttaaatct tagagaccca 90420cattttcttc ccccatacgt ccaagttttt gagggacatg tggatgcctg ctagtattgt 90480ctatctgtct atctactaca aatatgtggt tatatcacac acacacacac acaattgtag 90540gccttcagag gccagtggtt tggaataatc aggaacaact gagatagtcc tgaaagactt 90600ggaagtctca gaagtagaaa tgatgaggat taaaagtgtt gcaggggtag aaaaaagaaa 90660gaataaaggc caagagggag gaaagaataa aaccaatgga ctatagctag agtttggagt 90720ctgtataggc aattagtggg agacaaaact ggaaagaata gtttggacag tactgtagag 90780ggctttgaat accagagtta aatttctgtt cgatggacaa tgggaagaca ttgatgattt 90840tctagttgga gaggatttga tcacagctgg gattggaagc tgcacctgtg atgggcagat 90900atctgtaaac actgtcttag tctgcttggg ctgccataac aaaggtgtaa acttgatggc 90960ttaaacagca gacatttatt tctcaccatt caggaagctg aagattctaa gatcaaggtg 91020cccccagatt cagtttctgg tgaaggccct tttcctggct tgtagactgc tgtcttcttg 91080ctgtgcccca catggctgaa ggagagacct gtgggttctt ctccctcttc ttataaggat 91140aataatccca ttataggagc tccaccctca tgacctcatc taaacctaat tacctcccaa 91200aggcctcact tcctaatacc atcacataaa gggttggggc tcccacatat aaattgaagg 91260gaggcacaaa cattcagtcc gtacatgtac catctgggtg tctgttatta ccataattca 91320aatgagagga agatctgaac aagaatgaga gtgaagcaag agcaaaaggc attgcagagg 91380atgaggaaaa gaagagttca cagactctga aatgttgatc cagggtgagt gtatcaaagt 91440gccatcactg gaattgtggt taagtggtta gggatgtttt ggatgctcag ggatgttgaa 91500ttcaatttaa gttatgtgac ctgagggcag aacatacaga taagtgtaag cctcaagaga 91560gagagattag gttcaataga aatgtactgt gagcaaaaaa aaaccacaag ccacatataa 91620tttaaagttt tctagtagct gcatttttaa aaagtcaaaa gaaacaagtg acattaattt 91680gaataatata tgtttattta acacaatata tagaaatatt attattccat tatggaatca 91740acatattcta acatttcagt gtgctttggc tacatgtcaa gtactcaata gctgtatagg 91800tctagaatgg gaaacttagg agccagttac aaactcagct catttctttg cttgggattg 91860tgaacccatg tttgagttgt tcctgctttt tttgctatta tgaacaatgc tgttatgaaa 91920cagtcatata cagtactctt ggtgcaggaa ttctggacag tatattccta ggagttgcat 91980agctgggaaa cagcaggtga aatagttcca atttgttttc tttttttttt ttttttgaga 92040cagagttttg ctcttgttgc ccaggctgga gtgcaatggc atgatcctgg ctcactgcaa 92100cctccacctc ccgggttcaa gggattctcc tgcctcagcc tcctgagtag ctgggattac 92160aggcatgcac catcacaccc agccaatttt gtatttttag tagagacagg gtttctctat 92220gttggtcagg ctggtctcga actcctgacc tcaagtgatc cgcctgcctc ggcctcccaa 92280agtgccggga ttacaggcgt gagccaccac acctggcctg aacattttta atatgcataa 92340gaacatcttt ttaattcaaa tataaataca tattatataa catactgtca tttgtgtgtt 92400gtataatata ttccttcaaa tataaaaatt tgttctcgca caaatacata taactcatat 92460actgtcattt ttatgttgta taatatattc cttcaaatat aaaaatttgt tctctcacaa 92520atacttactg aacatctgct atctgctacc tattgatcta ggcacttggg gaaaaaatag 92580tcccaaattc ctgctatcat ggagtttaca ttgcagtgta tattgggtgg gggcaaagaa 92640aagtataata ttgtacatca tatggtatta gtctggctca tccagaagga agtggtaata 92700ctacctgtgt tatgctgttt attaaatgca tatgaagaac tcggcaccat gtctgctaaa 92760tagtaaggat tctgaaaatg ctagtggcaa taataataat aataataata atatttcctc 92820ctttcttgcg tctacttttt ctgcagtaat ttttaaaatt agaactactg gaaatttaag 92880attaagatgt ttttgttatt gttgaagtgt tggctctcat ttgggtgaac ttgtggtgtt 92940ttctgcaaca aacatgactt tggctcattt tgagatgtgt tattttcaag cttcttaaat 93000tccaagaatc aagagcctga agacatgcat gttttaaaag ctacatcatg ataaatagcc 93060acaaatgtgg gaaaatacct aggaatgtct tgtataattc aggactgtgt tatccaagta 93120taaatgttgc ttgaattaat catttcaatc caacaaccaa tttattgaaa accaactatg 93180tgcaaagtac agtgtagagg agatgcatat gaccttacta atctttgaaa caattatttg 93240tccccttttc ttagataatg atgttgaaaa tgatcttcaa tcagacatca gttgagcact 93300gcaagacaca cacggagaag cagaaagaat gcaacttctt cttaatagct gttcgcctgg 93360cttcactgaa ccagatcttg gatccttggg tttacctgct gttaagaaag atccttcttc 93420gaaagttttg ccaggtagca aatgctgtct ccagctgctc taatgatgga cagaaagggc 93480agcctatctc attatctaat gaaataatac agacagaagc atgaaagaaa acacttaact 93540tgcatgtgca cagcttttgg taacaaatat cgctaaacct tactgtgaat ttaggcatct 93600ctggcatgcc actgtttatg cattgaagtg gaatttttgg tataaagcta aatggtctta 93660gaagcataga aaatccctat gtgccaaaag tagtgaaaca caaacaaagg aaaatatatt 93720aataacagtc tagtgttttt gttgagtctg ccattcgtag ctgaatatgt gattaattat 93780gtgatgaaaa cattttttat aaatgatctt ggtctattgg ggagcgggga tagttaatat 93840tccagtacac tgaatacatg aggaatttaa ccacatacat cattgaagac aagggatagc 93900agtttgtttt tattcaaaga cattgctgtg ttctctttca ttgcctctct cgctttctgt 93960cacttttttc ctccttacat taaagaaaag tttaattaca gttaaaaatg tataatgtat 94020ttataatatt catcgatacc attattcaaa tattgctcaa tacagcaaat tagctcctaa 94080cctaacaaag tttaagttta cttggattga taattaggtt tactctttat ctgaataaga 94140accaattcca tttgtttgaa atatggagtt tgtgactacc caaattgcta attattcttt 94200cttttgaata tattttacat ttctatgagc ctaaggaaga ttcatgaaac tgacctatga 94260gagtcgtgaa gtggtttttc agaatgctat gtaaggaccg atttgagcac taactatagg 94320tactctgaat atatatttcc cttgattatt caccaaaagt gttccccagt ctttgactct 94380ttaaattcca atactgattc caaaacaaat aaatattttg aagactcaat gaatactttc 94440catattttgg cctatttata taagaaagtt aataacattg acccttcaca gctcttctgc 94500ctgctcctca aagtggctct atctaaatat ttattactaa aatgtttttc ctacagtcta 94560catgaataca aacctcaata gctaagcttg acgtatttgt gcacaagtag atcactacat 94620taagttttgg gaattgcact tcttaaaaat gtctccccac caaacatagt aatcctgtag 94680ttatgcctac acaaagcttg ccatattctt tggtgcattc attttgtaaa cccattaact 94740ttttattgtg aagattttca tttgcagttt cttgcactgc ttttctagtt ttttaaaagc 94800ttgagattta tttatacttc ttgtagtaac tgcatatttc tgtgtgtgtt tagtggtaaa 94860gaattaattt tgataggtac aatatgtcta tcagattgat atatacacca gcctatgtca 94920attggggcta attattttaa atgaccatgt caaattgaat ttggagacaa aatctgttga 94980gagtgcttat gtaattaatg atggttctac taatctaaat tttggaaaag gtgataaata 95040gactatacta aaatctctct atgccataga attggattat cctgtaggtc atctcattgg 95100gtataagaca aaactaccta ctttttttca aaagtgcact gaaatcacat aataaagagg 95160ctttacctct tggttggtcc tgtgacccta agttctagtc agatagacac agaggcaatg 95220tgaatttgag tggcatgagc atgattaggt tattccttcc agcatctagt atagcacctg 95280gaatatagaa actgtctaat acatatttat tcagtgaatc aatgagcaga agtttgccag 95340gacagtacac attggcaagg cacataccat atgattgaag tgcttcatgc cattacagtc 95400catcaggctg ataaagtgaa ttatttctga ttatttaatt acagaaatat gaatttatct 95460tcaagggtgt agtgtcatac tgctgtacaa cacagtgctt tatttatact aataatttag 95520gagactgata cttccaaatg atagtggaca ttactatcac aagaatatca cttttcatca 95580aactgcaaaa atacagaaag gcaaaaaacc tgacacttat tcttaactgc aaattaaatt 95640cctgcccagg ggatatattt taggagggga tgaatggcag cttttgtgtt ttttttaaca 95700agcttgaaag ggaggtggaa aacaaagaaa ttatgtaaat ggcatatgag ttttattatc 95760taggcattcg ttagtatggg gaaacctgat aagaaatgaa aatcccaaat gatttcagcc 95820ttttcatgat ggttgaggtt agatttcaga gatgtacaga gactagagcg gtggttagaa 95880agaggatata tgtagtcaca gcagaaagac gtgtctaagt ttaattttat tggctttcaa 95940gttcactcat gtatacttag tttgtccata catatgtcta atcaggaaaa atgcatgtat 96000agattatgac aattcctgaa ttttgaagta ttggttaaaa gacaattaaa ggccaagaaa 96060accatggtgg aagaagtaag cgaatgaaat gtagaaatat atgtaaaatt agcaagtgtc 96120aattttacca agtagtgttg attttccaaa caatgaattt atatactatg ctgagtcaca 96180gagaagaatg atcacatgtt acttaatgag agcagtttac ttttcaaata aaataggtat 96240gatgaatgtc ttaaaaatat cttgaagttg aagaaacaaa aatgagttat ctcaatattt 96300accaagttaa cctagtgctg tatatatccc aagatatttt aggtaaatgt aagtgtttaa 96360tcatgccaga tttaaactag tctgaaatat agggtataca tatatttcta cttacatttc 96420tttattttat gaaatatccg accatgttgc agaaaataat gcaaaacctc atgtaagtta 96480actatgaaag atcctgtgag cacattggca ttgagtgaca gacaaactaa aaactggcaa 96540acagtatttt aataaggggg tcactctgtg gcagtattct aatattggat tttcaagtag 96600attaggcttt ttatttattc aacgcttttt ataattttgt tctttttgac tccaaattat 96660tggtcagctt tcaaccttct ccacatcagc aatcactaat agttcttttg gttgagatca 96720actcagaaaa agaaaataga aaacttttgt tctttgaaat tttagacatg cataatatct 96780atttattttc ataatttaac cccaaaagct tctcctgcaa tacacaggat tctaggagct 96840gaatgacaca gggagactac agagtattta ttattacaaa cacataaaaa gcctaacttg 96900aagaattaaa atttctattt tttatctgta taacaagtac aaaccatcaa caatgacaaa 96960ttttcacagc tgcttgttta ttgcttgttt tatatgttta catatctcaa aatctgttaa 97020aactgaggtc taaaaaatgt gcagaattgt gcaactgtgg cctagtccat aagacttttc 97080tgagttgcaa caaagtgctg acaaagtgac agatgtctgt gatgtttcat gacataggtt 97140atgatcgagc caatttacaa aatttaataa ccaacctaaa ctcacataca tatttgttaa 97200gaagctgaca tcagttcagt attgtaagga aactaactag gtggtgatga tgataaaaga 97260attagggaaa atattttatt tgatatattc ccttttataa ttcctaaaat gaagattcta 97320tttaagggtt ataatttata taagtttagt catataccat tacattatga taccataagc 97380agagtgcatt atgattctct agaaatataa ttcaatcaga tatgtattat atttatttat 97440gtcacacatt ttctttactg agaataaaaa ttatcttatt ttcagaagct ttgtatcaat 97500cagtttcatg taataagcaa cccagatatc tactagatta tgtatttctt catttgaaac 97560tacactgttt tctcctagtc cccttgactc tactgtgctt atccattctt tcacagaaag 97620aaagtaacag acataattcc tgttgatgag gctgggattg tttttaagag gagagataat 97680aacttcatat ttttaaagtg ccagtagcct aatatgtgaa acagatcaga atctgttgtg 97740tagtaagtct gctttgttga agaatttatt atgggagtaa agataagaag gaaagagatc 97800accatcagaa acaagtcagc cttttcatgc ttttttgagc atttttggag atgattccac 97860ttctcaagtt attatcattt gtgcatctct tcaatgctat tgttaaatgc tttagaatta 97920gaatattttg atcctttaat taaagtaagc caaacgtcta ggcaaaaaca gccaatcatt 97980aaactttaat agtaattcaa atatagattt ctcatacagt tttccatgtc tgtagaaatc 98040aaagttgtaa tgttaagcag agggaaatgc gtgtgattta ctaatacact tcaacgttct 98100acttttgaaa ggatactcat gtgggtgggg cagagaacat agaaaaagat atgatggaaa 98160acctgtccat tttctacctg ttaaccttca tcattttgtg ccaggccctg gaagcaaaga 98220gaggaaggga ccgactgcat ttatctttga acacttgagc atcagtagta ctactgagtg 98280gccaggggtc ttgtctgtca aagcaaatga taagttcact caggccatta ttgactgctg 98340aactctcttc cttcccaact cttccttgaa agagaaaaaa atactttgcc ttcttgctct 98400ccttatcaaa tgtttttgta caaatagtgt aagcctgttt aagcaaacca attaaaatag 98460gcactgatta ttttgatctg tttgtaacaa atgaatgtaa gtactattta catggtgtgc 98520ctaggaggag ctgaaatcat tggcacttta atccatattg taaagatcag tatcaaaagc 98580atagtgttct tcacctctcc tcctcagcat ccatctctat atacttgatt aaatggaaaa 98640gtctctttta tcacctctat gtaaagtttt atgggtagtt atcgtcagtg tatttaaata 98700tatcttctag tatgttttaa aggctggtct tcaatactgt ggagacaaaa aataaaagag 98760cgtatgaaaa gtacgttaga cttttgctgg cattcaagtc atggctagtc tgtgtattta 98820ataaatgtgt gttatttatg tcgtgtttgt caatggaaaa taaagttgaa tattctgaaa 98880tgtcgctgtg ttttcttcct gatgtcaact cacgtcagga atactttacc tataactatg 98940ttaagtattt tgctgaaatc ccatttgatg tgctttgtca aataatagca caatgtaatg 99000caacagcccc atgctatctg gaaaaattag atcattttgt tttatattcc ataatcatgt 99060gcataacaaa ttattgttga tttaatataa atatgagagt tgctcttttc cgattttcaa 99120catgatgttt ctttgtttta ttatctcaag attattgttc ttaacacaga gtgcatttat 99180aaattattgt aagcttagtt atttcccttt cttgtggatt ttggtgtaat ttacaggcag 99240ccaaaataag aattgtcctt gctcatagta caacccttta attgacttgt gcctgagttt 99300tggtgtgagc tgagtattgg tggttatgaa tactgggatc cccagctatt tgttcatgta 99360cttattgagg tcactattgg tcttccttct tcaatgaagg gagaagctct gtcaaatgta 99420gactgcaaag tcaagctcat taaaagtatc cctctttctt cgtgagaaaa gatatccaag 99480gagaattctg tccctggttc ctcccatgtg ggggttctag gcctaggacc agatcttatt 99540gtctctacaa tcttgattct ttagtatttc aggataggca gagttttgtt aaagtataca 99600aaaatcatag ctagataaga gagataacag aaataagttc cagtgttcta tactcctgta 99660ggatgactac agttaatgct aatatacagt tacaaatagc tagaaggagg ctattgaatg 99720ttcccaacac aaagaaatga taaatgtttg agatgatgat gtgctaatta ccctcatctg 99780accagtatac attatatgta tcgaaacatc gctctgtgcc ctgcgaatat gtacaattgt 99840gattattaaa ataaatacat aaataaaatt taaaagtcat tatgactact atggctatgg 99900ctcacccgaa atttccacca tcatgctggt aaacctacag gccatggggt gagacattcc 99960ccagtttcat gtcctcagag ggcatggggc caaatgtcct tggggccagt gtgcttgcta 100020gtactagcct tgagtaaatc cattctggat ggatgtttgt catggagaat ttaagtcact 100080tctgcaggct tgctctttcc ttcaacatat atttattgca tacctattaa gtgccaagct 100140ctggtaggta ctaagaccca agaattaata aaaacagcaa gctctcagtg aatttataac 100200ctggtagggg aacaggaaat aatcagagct gcaattagag taagcacatg gtatcatgag 100260aatacatgga aacgtgatgc taacatggga ggtattcctg tagaaggtgg catttaatcc 100320tcattttggg gaaaggaata ggtgttaacc tgtaaaggaa ggacattgta ggatattttt 100380tcacttcact ctgtccaaag cattacagtt tccaaatctg tcaaatggac taaactttag 100440tggtcctgtg ggtagtgtgg ctctacatag atcctttcta tataacctct cagggtcatc 100500aaagtggcat ggctttaggg cagggtttct caaccttggc actattgaca catgggacag 100560gtaattcttt gttgtgaggg ctgtcctatg cattgtaggt tatttcccaa tgtccctgac 100620ttctactcat gagatgccag caatacctct ctgatgtgac aatcaaaaat attcacagac 100680attgccaaat gtactatggg ggacataatt gccctcagtt ggaaccactc cttaaaagca 100740agggccaggg ccactaaaca ggtgctatgt acccaagggt ttgaggggtg aggccatctg 100800tggggccttg gtccacatag gcactgttct ggcatttcct gaagcagcag gtactagaat 100860gccagtttgt gtctgactgc acaaaaatgt ggtgctgcca ctacaacaat gcctggatca 100920ccacaggccc tgatcagctt ccataatcac ataaacctat tcacagatga ttaggcacaa 100980tttcttagaa agtatcatct ttctattgat gcctccaatc tctattctct ttttgataaa 101040aggaattagg gtttgtatgt ctctcaaagt gatacctaaa gtgagtcttt gtctccaaag 101100acctttaaaa aatctgttat aattctacat ctttaagtac acatttccag acaattctct 101160ccgaaggaga aaactaattt atcaaactat ttccttaaat tagatgtttg catatttagt 101220ctgtataaca aagctgcaga agcttcattt tatctgaaag atgattttta tcaaagaaca 101280aatattgggc aatgtctctt gatgagcctg tgactttctg agttcatgcc agtcttcaat 101340tcctcaggtc tagagttatt atcatacatt ttgataacag agttcaagtc atgatctcta 101400taactgacac tactgaaacc tattgtttgt ataaaatggg aagatgggtt tacctaccac 101460actgaaaatc aaagtctgat aaaaaaatac aattagaatg agggacctaa tctgttaacc 101520ataagtttct gaggccaagt tacaaaagac tatggataat cagtgtttat gcataatatt 101580cttatgcaat ttttaaaaag aaggctttga atataacata caaggttgtt tgaatctgat 101640tttgcaaaga acattgagag aaaataattc tgaaatccac aattgaggct gttttaagtg 101700atatttaatt agaaagaaca ttggttctta attattttag gatcaacgac ccttctgaga 101760aattgatgat aggtatgtgg tactcccaca aaaggtgcag aggatttttg cacagtgtca 101820gaaattgtac agacctgccg aagtctatct atagacctct tcggcattta tgcccatagg 101880ttaagaaatc cttctgtgag agcctctatg atttaaggaa ctgcaaagct ttccacgttt 101940ggttaggaaa ctcaaaccta aaactggaac taagtcaatg taattacttc ccagtgaatg 102000ccaaaattcc ttgacatcta tctaacgata tgtccataac agtaaatcaa tgtaactgga 102060ggtagaatta ttataaggaa ctgcaatcat ttttctctaa aagttgcaat catgtgatga 102120taagaaataa cacaatttga tctgagagga gcaatatagg ttactcaccc caaaacaggt 102180tgaaaagtga aagacccatt taaaaaaaat attacaagtt atccaaagat gcatttgcct 102240tacaataaag atgttgtttt agttttgcct tgtgctggcc caacaagaga gatttataat 102300gtagctattt aaaaaatcaa taaaaatgtc atctacccac ctccctggaa aaatgtaatc 102360tcctatacac aagcaagcta catggttaaa atgtaacttc atgatactag gatatatatg 102420tagacacata tctatttata cttctatagg cactaaattt gtgcattgcc attcagagtg 102480caagtggcag ggatgacctt attgattgaa cctttgaata agaaagcaag gcatgcagtc 102540acagaccgtc ttacaaatgt ataattaata atggtttaag ccaactttta aagctattag 102600tagaatctta ctgatgttta tggtttgcat ttctctggag ggaaaaatgc agagcaatta 102660atgacatgta tttttgcatt tgtctttcct tattaaagaa gatgaaaatg gaatcttaga 102720gcattgaagt atttgtcaat cataaacagc tctttgatac caaagcatcc tacattctgg 102780atgtatgtgc atgaattgga cagatacaca gtatgtgcat gacattacaa gttgatttta 102840atgagtcagc agtggtctgt gagttggagt cttcttctta ggagatccag gaggagctgt 102900ttttagagga aaagcaattg tcctttgtta gtttcaacat ttcttgacca tttaaatttt 102960ttgcccaacc cttttatttt aagaattaac gggacaaaac gggtttttac ttgaaatatt 103020ttggagaaat aatattggca tatcatgaag tgtaatcact gccttaaatg ttgggcagct 103080ctaattagat atgcatgact cttatccctg tccgagattt tctcacacca tgaccgtttt 103140gaatatccct gtgactattc attcagacta ttaataatcc aaagctgaac taattcattt 103200attttggcca tatgtaattt aaaatatttt aaagtatatt ttaaatacag tcattttaca 103260aaaatgctct ctaaagtttt gtgtgtcata atgaaagaga aaatgttcta cattggacaa 103320ggagccagaa caccttggtc gaattatttg atttgttatt tactggttat ttgcccttga 103380caatccatta aatgtctctg aatcaacttg aattttctta tcagcataag attgttacaa 103440ttaaatgaga taatgaaaat acagactttt tttttaagag atagggtctt actctgtccc 103500gaaggctaga gtgccatggt gtgatcataa ctcgttgcag cctcagcctc ctggcttaag 103560tgatcctccc acctcagcct ccccagtggc tgggactaca ggcatgcgac acctcactag 103620gcttattttt tttatttttt attgttttta gaaacaggtc tcactgtgtt gcccaggctg 103680gtcttaaact cctggcctca agcattcctc ctgtcttagc ctcccaaagt gctgggatta 103740caggtatgag gctttgcacc cagtaaagac acttgaaagt gctatacaag tggttagtgt 103800tactttgtta tattcatttc attaattgaa caatattaga aaattaatta tgattgttct 103860catctcatgt ttactacaga cttcccagtt tagtattggg caatcgcaca ttcaccagaa 103920cacacaagtg acgttagaaa gctggttctt gtcacagaca ggatgtgtga acttgggaaa 103980aatacacagc ttcttttagg cttagtacct catctgtaaa ataaggggat gatatttcta 104040tgtggtatct tctcactgaa aagccatgat tctcctttct ctctggaaat gtcatgaagg 104100tggtcttgtc agctaaacac agataagctt ttctcttctg agtcctggaa aacagctcac 104160aggaaaactc taggagacaa aaatagcaag actcaaaagt atagattctt tttttctcca 104220aaaagaaatt tcaggttccc actctttctc ctgaatgaga tggatttctt ttctcaacca 104280aattgcaaaa tccttgaata caggtaccat gtcttccatt tgttaagatt ttctgcctcc 104340ttttgcttta ctcttcatcc taactgctaa atcctatgcc tttaattgtt aaatgatgga 104400tattcattta tttgtggcta aggaactctt gtaacaaaga gtaagttaca tagattttta 104460tacacttgtg cttatttgtg tgtgtgtgaa ggagcaagag agatatctgt tatacctcaa 104520atgtgtattt cttttcttaa gttttttcat aaatttttat taaaatacta tgtgcatctc 104580tttaaaaagt caaatagtgc tgaaaggctt atcatgcatg aaacccacag tccccattct 104640aaccctaccc ctagaccacc tgctttccag tggctactcc tttcagctta ttttttcaat 104700gttttttcta gcatatgaca ccataatttt aaataatata ttgttatctc acactcaatc 104760aactttatag acattatatg ctgattttct attttgctgg atttagctct ctcctcaccc 104820attctcccat tatagttata actgactttc attaaatcaa cattcaatgt ttgcattttc 104880atgataatga aaatatcctt cactgaggaa tcagtagtac tgtaattatg tttcttttct 104940tgaatacatt tctctttttt tctagagtta cgaactcatc aagttttgaa tttgcttaag 105000tttgctttgg aacagtggct aaatcttaat agtcttccaa gtgcccaaac ctatctgata 105060atctttaccc actttgggag tctaacattt ttctggtttt ctctgctatt catccttttg 105120cctcttttat ttatattgat agtcctttgt ctagatccca tgtcttcatc cttcttgaat 105180tattcactca ttttgaccac atctgcttta aaatgagctt cttaatagta aaggatgtta 105240agtaaccaaa ctcccagaaa tggaaagaga atgctttcag ggacacatga acactaacaa 105300cgttttaaag aaatttgaac tggtgataat gaccatagtg gcagatacca ttttatgagc 105360acctacactt ttcgtatgtt atcaaattct catgtcaatg acagcaggta ggtgttatca 105420gcactttcca gataaggaaa ctggggtttg tggaagaaaa gtcacttgac caaatacaca 105480acagttaatg agaaataaag ctggggttca cacctgactt gggtttagaa tataatcatg 105540tgttatccat gagcaaataa ttactactgt gattagtttc agaagcttca agtttatctt 105600cgagggtttg tttaaaaccc catggaagaa aggctttgac tctattatca atgtctggac 105660attttcaaac caccagaaat aattttgggg agcagtggtg aggcagcttt tctaagagtg 105720ctgattagat taagcaaaca agattctcag caaaaaatgt gaaggtccat atgtccaatc 105780taggtgtgtg cacatgactg caaacattta gatccttcct tgtttgatcc aggaccactt 105840ctgcagtgga gaaactattg gtttcctggc agcagatggt tgtacagaaa aactgcactt 105900ctacttccac acaggcctgt ggtttttgct aaagccaaga gtttatcaca atttgggtta 105960ctaagaatac atctcttttg gccctattgg cttttgtctc tttttttatt tttcctttta 106020cccgtcctct cggacttgta atttgggaga atgcttactg aaaagcagaa gatacttgtg 106080cttcctagtt acagaggaac tcctaagaga ctgtagaaaa ttcaggacct agggcagacc 106140atttgtcatc agtgaaacta ttctgattat catctctccc tgcttaattt tgaaatttgc 106200tttagcttct tctggtaaaa tacaatgcag tttgagtgga gcacattttg tctttcagaa 106260agaaaaattc ttgccccctg tttctgagtt ttccatccct ttctgctaac aggtgaaatc 106320tatttgatgc atttgatatt ttattcattc accagttttc tcttcaactg actgctctct 106380gatttccaca gaactgatca gactgattag aactgtgcaa aacattttgt tcaaggaaac 106440aaaatctcag cgtctccaaa ctctaattct attattatca ctaaatgaat tcagaatgca 106500acttatccat agatatataa agtggaaaaa tttgtacatc ttgatgttta tctttagtct 106560tattttgtac attgtctttt gaatatttca atgtaagtgt gacaatagtc aacagttctc 106620aggcatctct ggggtcaatt gacatttatt tgaatgaatt caacccgagt agctgggact 106680acaggcgccc gccaccacgc ccggctaatt ttttgttttt tggtagagaa ggggtttcac 106740cgtgttagcc acgatggtct cgatctcctg acctcgtgat ccgccctcct cggcctcctg 106800aagtgctggg attacaggcg tgagccaccg tgcccagcca cacgccagat tttaaatatg 106860gtacttatcc accgtcatca taataagcac catcgtcgtt ccctgtgcaa aattttcata 106920gatgtatcga gattcaagca aaacagtaat ttatttctga gtacctcagt tcatctctag 106980ctgtaaagtc acaaacagaa ctaccagaga atttccggac tgtgatgtat ttgagaaata 107040cacaattaaa aggggcatta aatcaatttg aattaggatg gaagtgattc ttttcctctt 107100agtacttgga ttcacagcag taacaacttg gttcttagtg gagctgttac ttcccaagtg 107160atgactgtgt ctccaagtag attttgacca ccaattaaat gaatgatttg ttgatttatt 107220tagtcatatt cacattttta atgagcttct atcatgtggg tctaggttgt ggggacattg 107280aggtttatca cttgcagatt tcagatttca gattctctca ttcttcagag cgaagtctca 107340gttttcactg attgcattta ggacttaaat agctttccca aagtgtaaat cgctctacat 107400tttctttcat tgtaattaga atatatattc atgtataaaa tttaagcaat acaggaaagc 107460acgaggacca aagaaaaatt ccctatactt caccactcaa acttaatcat tgttatggtt 107520ttgattaata ttctttcaaa tatttgtctt tgtaaatttc ccttcaaacc tgactgcatc 107580taagaaccat ggtgagattc tctcaaatgc atgaaggagt gtcacattaa atagagattt 107640atttcatatg ccctgaaggg cggactaaga agctacagag agaaagagtc tgacttaatc 107700tagaaaggtg ctaaaccaat gtgccctaag gtggcagggc tgaccttaag caataatgag 107760ttccctgtca cttcaaatat tcaagcacat accagaaaac cactagggag tttgctgtag 107820atggatggaa tacaaatatc ttctttaagt cttcttccaa ctcaggattt tatgattcta 107880tgtctcaaaa cagtcatact atggctacaa tattggaatt ggtgtaaaag acaaagattt 107940gtctgaatat agctgctgag cttagtgttc tgctttcaac ctcttcagat gtggcttata 108000ttctgcttta tgtgtcacta aatgcattgt tgttgaccct aatctcaaag ctagaatttc 108060atgatttcta tgttcacttt atcagtttac aacatttttg gaaaactact cataaaacag 108120caaaaatgtt tttttaaaaa gagctacagc aattactttc caataattaa atcagaatat 108180ctggtggtca ttaataacaa ttataattgg attattggat tgacttcatc gaatatgaaa 108240gtctgccaac aatatgaatt tgatcaaact ctgcttatta tctattctga tcattttttg 108300ctatgtgtaa aatatacaca tatttcagaa gaataaatct atcatgaata acaatcacca 108360aatttttgta caatattatc taacctgtta tgtatatatt tttaagtttc ataaccaaga 108420gaagagacgt atactttatt tatttactta ttttattttt attttttggg agacagtctc 108480cctctgtcac ccaggctgga gtgcagtagt gcaatctctg ctcactgcat ctttcacctc 108540ccaggttcaa gctattctcc tgcctcagcc tcccaagtag ctgggattac aggcatgcac 108600caccaagctc agctaatttt tgtattttta gtagagacag ggttttatca tgtttgccag 108660tctggactcg aactcccaac ctcaattgat ctgtcctcct cggcttccca aagtgctggg 108720attacacgca tgagctacca cgcccagcca agaaatatac ttcaaaacat agaaacaggc 108780caggcgcagt ggctcatgcc tgcaatccca gcacttttgg aggccaaggc gggcggatca 108840cgaggtcagg agatcgagac catcctggct aacatggtga aaccccgtct ctactaaaaa 108900cacaaaaaaa ggctgggcgc ggtggcgggc gcctgtagtc ccacctactc cggaggctga 108960ggcaggagaa tggcgtgaac ctgggaggcg gagcttgcag tgagccgaga tctcgccact 109020gcactccagc ctgcgcgaca gagcgagact ccgtctcaat aaataaataa ataaataaat 109080aaataaataa ataaataaaa atacaaaaaa attagccggg catggtggcg ggcgcctgta 109140gtcccagcta ctcgggaggc tgaggcagga gaatggcatg aacccacgag gcggagcttg 109200cagtgagccg cgccactgca cttcagcctg ggtgacagag ggagactcct tctcaaaaac 109260aaaaacaaaa tagatacaaa tgattcagta aatgtttagg gaagatttta aagtttaaaa 109320taattataag gaaccaatac cattacttaa acagggaagt ttttttaaat caacagtaaa 109380attattctta aagacattat tttgtctcag agagtaggat agaatgggta tttttttttt 109440aggtaagtca agcctaatta ctacttaact attcaaccta attcttaaca ggattagaaa 109500gagttacatc tcttttaaag cacaaaaaac cagacactca catctttgtt ggaaatgctg 109560aaatttctgg catcattaat tgttccctga agacgtcatt tcagtgtgcc actgtaaact 109620ccaaggttta tagcatcatc aataaggaag gcaatgagta gccttatgga agggatttgg 109680ggatttaaag acataaattt gaagtatggt tcttgactta ccagctgtgt gactgagtag 109740gttttaatct ctttcagcct ctgtttactc atctataaat tggagataac aacacctatt 109800tcacaagata gcaaggaaat gagataatac gtatatataa agcccagtag gaatgcaatc 109860atttatagac aatattaaca tcaaataaca ctaatttcct ttccagaact atatttagga 109920gttatattag ttgaatagtg ataaatgtaa aataatatta atagtgaaca cttataaaag 109980tattttgtac tttctaaggc attttaccat ctgtagtttt atttgatcaa atttttagtt 110040agctagggag tgttgttatt attcctattt tataaataat taaattgaga ttaagacata 110100tatcaaaaca tcatgttatt actataattt ttacttgtca attaaaataa ttctaaaatt 110160tttttaaaga aagactaaga ctaggtgtag tatttggacg ctagagtgaa acttcagctc 110220aaatatcact ttcatacttt ctttagagtg agtttcttac tccttctaat ctcatttttc 110280tcatttgaat agtggagata gtagagtgtc ctagactatc tatttcaaga gcagagactt 110340agagtgtcct ttactacttc cccactgccc actgtacaaa gcccaaaagc tttaacttgt 110400tcaaagctct cagtaaacca atcccaatct accattccaa tttttttctg gcaacactga 110460taccagcctt ttttgaatcc tctccccaaa cacattatgc acattcacaa ttctgtttct 110520agtgtttact ttgcttaaat gttaatttca atgaagaaaa ttctgctcat tcattaaagc 110580ccagctcaaa tgacacctac cctaagagtt gtttccttat gcctgattca gaattactga 110640ttccctcttt tgtacatcca taaacacctc atttatgcct cggtgattac ttctcactgc 110700tctacttata ttacagtgag ctgtgggtga ttctagctcc cccactggat ggttaccttc 110760ttgaatgaaa gacacatcat tttaatctta gtgtcccagt ccctcccact tcctctcctc 110820cccagacctt cagcttccaa gaatgtgaag gctttgaatt atagatacac agtagacata 110880gaaatactta ttaaattgat ttaaatgacc aaaaaaaata gtacaaataa aaaaaaggca 110940atgatgtccc agaagaggtt ttagtttata aaaattaagt tttggctggt cgcaatggct 111000cacgcctgta atcccagcac tttaggaggc tgaggggggt ggatcgcaag gtcaggagat 111060caagaatatg ttggccaaca tggggaaacc ctgtctctac taaaaataca aaaattagcc 111120gggcgtggtg gtgcacactt gtactcccag ctactccgga ggctgaggca ggagaatcac 111180ttgaacccag gaggcggagg ttgcagcggg ccaagatcgc actactgcac tgcagcctgg 111240gcgacaagag tgaaactctg tctcaaaaaa aaaaaaatta aggtttacag acttaccaca 111300ttgatagata cagcatacac tacatgtttc cgtaaatagt taactataat tagggactga 111360aattagagat aatggcatcc aaacttttgg catgcttgct attcacaatg cctctttttc 111420catcccggta aaggtccctt ggtgtcacca ttggagagaa tattaaattg aatgaactac 111480tagtcttagt gggtgcaaca cttcttacgt tcttatatta agtttctgag gtgatatgaa 111540gggaagacag tttttgatta attcagatgt ctgcaatggc cctagctgga gaatggccag 111600gctgagaggt gagcggccag gctagaagag agaagggtag ctgtggttgg caggaacaga 111660gcaactggca aagagcagag cagcctttgg ccgcagggtc taaacccaca tccttgctcc 111720cagccctttt ggtaacacag ctatgaagtc agaggctgat aaagagtgaa aaaacacttt 111780ccttccaaga acgaagagcc tttgaagaaa acttctgacc acctcctttt ggggcacaag 111840gaagttgaac agggttttga tctgttcaca tttagaaatt ctttttgctt cctgccatgt 111900gttcatatca ctagactgca gtttctccat caattcttct tattgttcac tgcatccctt 111960attctttcac tcactcggtt tgcattcagc ttctaatttc tgcttattac tatacttaac 112020cacagtgggg atgcaaatgc cctcgaggag caaagaatgc aagtaacaca tctcaaagac 112080tggaaaactc caaagcacac ctgagcagcc tcccagtcat tcctgaaaat ctttcttctg 112140agctctccag agactacaga gctttttgtt attaaccctc ttcacctaca cctcagtttt 112200gttaatgggc tgctgcaaaa ggagagccta acatggttag acattttagc ctaactgtac 112260aaaatgtaga cttctgccca agaccagttc acttttcaat acttggtgaa tttctcagaa 112320aatgagtata gcatgattcc tctagctttt agggtgtcat aagctctatc gcacagacat 112380attcacccat gtcccatgta tacacatgcg ctttataaag caaaccaaag acccaggagt 112440cttgcgctgt ctctctagaa gttttccctt taacaaggac accatctgtg atacatctgc 112500tttcttagtg ggggatatgt aacaaacgta ggacagtaat actgaaaaac aaatggttta 112560aagatgaatg tgttccacta tgcagaaaaa gagaagtgag gttcataaga gaatatctgg 112620aagtgggaag aaagtaagta tatccagatc tatatctaga tatacttgtt agaagagggg 112680ggaatttaac attgagggac ctcatatcta attttattga agaagaaaaa caaggttaat 112740tgaatttatt ttcaagtatc taatgaaatt aatctcaaaa agttctaccc agggcaatgt 112800tgttataaat gttttgggtc ttagttatac tgttgctata gtattcaaat tagcatggat 112860gatcattaat caaatgcttc gaagatggta gtcactgtgc taagtgctta gaagctgcat 112920ttctcatctc tgcttctcag agggaaatat atagtgtagg gtgaggcttt aattagtatc 112980ttttatttgg ttgagcccta aaggaaatca agtaaaatga tgaaatcgat gtccttggtt 113040gctcaagtgt tgcctctttt ctctctgtaa cactaggctg tttccacctg agtgcatggt 113100agagaaaaga gcacattttg agacatgaag ctgctaggtg tgtctacaca attggttaca 113160tagggtcctg tagctgaaag aaacagtaga gatcattgag tcaaaaggag ttcaactggt 113220tcaacagagc gctatcttta gtaaggggtg aagggttcaa ttttgaacaa catgtgggtc 113280gctataacag cagcctaggc tcaatatctc ccttttatct gttgtgtata tcaggctttt 113340aattaggatt tcatttaaag aaatcgctct gcttccaaaa aaacaaggat atcaaccaca 113400aattcagttc aactgagaga gtttaagtgg cttgttcaaa gttgcatagc tccttagagg 113460cagagccagc tctagaactc gggccttctg actcccagac taacttcagc cttgatgctg 113520tgtttgacag ctatctggat gaataaagag cagggattct ggagccagac tacttgggtt 113580caaaccccag ttcatttact tactagctgt gtgactgagg gtaaattact caccctcctc 113640agggctcagt attttcattt gtaaaggtat tagtactttc ctctgggatt actatgaaca 113700tcaaatgagc taaaatgtgt aaagctttta gaaaagtgct tgatatttag tataaccaat 113760taaatatagc ctattattat tgtttaaagg gtttccaaaa gctttcctaa ccttatagga 113820tccaccataa gacctataat ctaagaattt tcatttcttt gagcaaggac acaaaaggcc 113880cttcttagaa agtaaatgca ttatctgagt aataacacag taaatcactg tcactctctc 113940atatctcata aataattatt tccattacct ctagccactt agtaaggcca tagaggaatg 114000ggacatagaa cagttaagcc ttaatgatga gttagagtta ggatttcttt ggaaaaacct 114060gggaaagaca cgaagggctg ggagggaacg aatctcaaga gaagacagac acctgaagct 114120attgtagccc atttcaaaat tctcttctgg gcccacctca gagactgagg cacggaatgc 114180atctccctgc agttcccaga aaggactggt gtcagggtct ccttgtgctc actccctaga 114240aaccttccct ccctgtctca ctccacatat attctctata ccctagtatt ctggctcaga 114300gagtgtaatg aaagtagcag tggaaaatta aagaaagaaa gcaggctcat aagggaagac 114360tgagggtttg gagaggagag gataagagag gaaatgtctc accagtcagc ctcggggata 114420gtgggcagag agtgagtagg gtttccaaga aaggagcagt aaaaccaaag tgtaatgtgc 114480acaggccagc tcctctttct ccatgtttcc ttggactgtc aaaagtgtga gggtagggcc 114540aagccctgag tatatatcct tgcagagtgt cttgtttttc tagattgtat tggagagtgg 114600gagttctaat ggtatgtgtg tgagtgtctg agagagaaga attgagtgaa gtttaaggtt 114660aggtgacttt tattattaat caaatggaag cccaaggtca atgagaagca cattggaagg 114720aagcctatga agtgaatgac gttcagaagg cagttctcaa caataaattc aaataacctg 114780ctttcttttg agcaagaaat taaatgttac cttcactatt tgtgaaacgg aattgtagca 114840tttttcattc tgctccaggt aaacacagat aatctagtgt ttttattgcc attaatatta 114900atctccatat tatatgattg tggagtggta catatcagtt agttaaatat tttagttcca 114960gccaggtgca gttgctcata cctgtataat ctcagcactt tgggaagctg aggtgggaga 115020attgcttgag cccagaaatt caagcacagc ctgagcaaca ctgtgaaacc cgatctctac 115080aaacataaaa attagccagg tgtagtagtg tgtacttgta gtaccaggta cttgggaggc 115140tgtggttatg ccactgccct ccagcctggg aaacagaaca agaccctgtc tcaaatatat 115200atacatgtac atacatgtat ttatatgtgt acatacatac atgtatataa aatgcatata 115260tgtgtatata tatgtataat atatgtatat atatattata cattgataca tatgtataat 115320agatataata tgtattttat atatatatat atgtgtgtgt gtgtgtgtgt gtgtgtgtgt 115380gtgtgagaca gtctcgttct gtcgcccagg ctggagtgca gtggcgcaat ctcagctcac 115440tgcaagctcc gtctcccggg ttcaagtgat tctcctgcct cagcctcccg agtagctggg 115500actacaggtg cccgccacca cgcctggcta attttttttt gtatttttta gtagagaggg 115560ggtttcaccg tgttagccag gttggtctcg atctcctgac ctcgtgatcc gcccaactcg 115620gcctcccaaa gtgctggcat tacagccgtg agccaccaca cccagctgta tgtgtatata 115680tattttcaca tatgtacaca tacatacatg tattgtatat catgcccaga agcttgatgt 115740gtatatacac acatccatac atgtatgtat atattttcac atatatattt ttacaaatac 115800atgtatatat gtgaaaatat atatgcatgt gtgtgtgtgc gtgtgtgtgt gtaggttcca 115860ttgactggta atatgttttt gataattcag aaggcacttc aatattttgt aagtccaggg 115920gcatttttac taccattgat aactggccaa tactatcaaa gtaaatgctg ctatgtgaaa 115980tgtctggatg cttgattctg ggttgaagaa attttactgc atatagcact tggatttcaa 116040tcagcaagtg acatattcta actgcacagc cttctataat ttgttaataa atttattttt 116100attttcagtc tcagtccaag tgcttctcag ctagtttcaa aataagcagt tatgagttag 116160ccaaatacac attctcaata atattgtttt agctaaaaat atttgcatac atgtagctca 116220tgaaaactca gacatcataa aaataaaatt atcctgcaga accatgaaag tgagaaggta 116280gagaaaataa gttaatgcag gcaaaaaaga gtccctttta aaaggtaatt attccagaaa 116340actgaaaaca gagtgcttct cccagtgaaa cataagtgag caccaaaatg attttagtaa 116400caacaataac actttgacat acatttattt tatttgatta tcccaacttc cctgcgtaga 116460aaatggagct gctgttatca ttatccttat aaccaagctt tataaaattt aagcaacttt 116520ccaggtctgt agtgagttaa gtaagcttca agcattctga tttctgttct gagcgctttc 116580ctgtgccact atctgtattg taaacccggt aggagggcag agcagatttt tattacacag 116640tagtatgata ctgtgtcacc tctcctcttt aagaatgtca aaatattgta caagttttgg 116700ggggggaatt acatgcaaac aatttgaaat tatcatggaa cttctgggca tgatacacaa 116760taaaaccagc ctcctcatgt taactcccaa agccttggtg actgaacaat ttaggtaatt 116820aacttcattt taggctagct ataatgaaag ctcttggttt cctttataag tgtggcttct 116880cttcacatta ctttaagttt tctcttgata actgctcaga atttattctg gctgatggga 116940tgactttatc actatgttag gttttggtca gcttcagaaa aaaacaaaaa ataaataaaa 117000caatgattaa aagaacacag atgggtatct ctttcctatt tatgtaaatt ctggatgtag 117060atagtccagg gctgatctgg ctacttcctg gttatcagag attggagcta tttctagctg 117120tcattcttag tttacatcct cataatccaa tatagctgct cgagctccag ccattatttt 117180gccttctggt cagtaggaag gaggaaggaa taaagaaggg tgtgcctctt tcctttaagg 117240agaattttag aagtctcatc acttctgctc acaaataatc ggctggaatt tggtcacgtt 117300gccacagcta actgggagag agtctggaaa tgcagtgttt tagtcggtat ccacctcata 117360atcagggctc tggtagaaag agaggagagt ggatgctagg aagcaattgg aaggctctgc 117420catagtctga gaatggttcc ttattttatg tacccatatt gcatgacttt taaaacttcc 117480tatgggttat tttatgggaa agaatttatc aaggttttgc ctgaagatca cagttactat 117540gttattaaga ataagagaag ctgaatgata agtaatttgc tgtattactt catatttaaa 117600attaaacaac tgagttttta aacactttta tgtaagatcc agattccaaa tggtcatatt 117660gtgactaatg gggctttagt catttttatt ttgatactcc tgtcactgag agaaatgaga 117720atgcattgtg aatttagacg cttcataacc acaaactaca tacaatttac actacgtaat 117780gagagctgtt taacaataaa acatttttat tggtgaggag tcaaagtttt cttttaatgc 117840ctagcacagt gtcttacaca tgaaagatat gtaatcaatg agtgctaaat tgaattgacc 117900ttcactagtc agttggattt atttatcgga tgtataaaag acccttgaca gcttctgcta 117960gtatccaatc attattctta tggcagtgtc acactagctg ggatttaatt tgattatatt 118020gggtggttgt ggttatgcct atgcatagct gggaccacag ggaacaaatc acttctcagc 118080tgtgttaagt gttttgcccc tgacagtgac cacacctgca gcttcaggga ctaggaaatc 118140ctccagaaca ctgaagtgca tagagatcaa tcaaatcagc tcttacttgg agaataagaa 118200tcgatttatc atggaactac ttagtgcaat taccatattt attatagtct tttatttaat 118260ccttctcaat tactgatgtg gtaatatact agaagagtgc atttaatatt ttctttttat 118320ctgagctata tttcttgtaa tttaccttgt aaaaaactgt agataaaatt gcaatgagac 118380ccttgattga ctcctattac tgattataca acaaaacata acttatacgt tttgtcagac 118440ttaatttgtc tttagacata tattcttaac ttgtttggaa ttagaatggc tgttcttatt 118500tgacattgtt tccatttcct tcctggtcac ctttataata catcaaactg gaaaagaaaa 118560ggctcacatt cacagattta taaaggggag aaccataacc atatttgttt agggccattg 118620atatctgttt ggctcttaga gtctctgttt ttgctaatgg ccagggtgaa aattatacag 118680tgtacaacta ggctgtaata caattatagt aaatactaat tgtaaaatta atacaatttt 118740aggtgcttaa agatatctgc ttaaaatttt tctagaaaga acaaaactct tacagatgtt 118800tggtctctgc tggctggagc aggagctagg aggtagaaga gtaggagatg ggttcttgat 118860cttgcggttg aagatatttt atcaatccat tattttctca ttttgggtta attcagtctc 118920tttgaggcaa atgttgtctt tcatggtttc tcccagcacg ccctttcctt agccaagatg 118980gcatctgtgt gaaggagaaa gctcacatac ccttgtgata atgcaggtaa ttgaagggag 119040gcagcttgtc aaaagcaggt cctgatcctg ccctttgggt cctccctgaa atccccagaa 119100tcaattgttg tcttgcctgg ttgctagaca gccactggca tccaccattg atttcagtgc 119160tggtgaaatc tgggaacagt ttcctcaaca tgttctagcc ttgtggtcct gcttccttga 119220ccccactgtc atctcagtga cacagaagac ccttgaggca cagccacttc cttcctccag 119280tccattgcct taggctcgtc ccctcatagg aactgctgtc tctgccatgg cgttacagga 119340aaggggtccc aatccagatc ccaaaagaca gttcttggat ctcaggcaag aaagacttca 119400gggtgagtct acagagtaaa gtgaaagcaa gtttattaag aaagtaaagg aataaaaaat 119460ggctaatcca tagaaaaagc agccccgaga gtcgattgtg gcccattttt atggttatct 119520cttgatgata tgctaaacaa agggctgagt attcatgcct ccccttttta gaccatatag 119580ggtaacttcc tgttgttgcc gtggcatttg taaactgtca tggtgctggt gggagtgtag 119640cagtgaggat gacctgagat cactcttgtc gccatcttgg ttttggtggg ttttggccag 119700cttctttact gtaagctgtt ttaacagcaa gatctttatg acctgtgtcc tgcactgatc 119760tcctatctca tcctgtgact tagaatgcct aaccatctgg gaatgcagcc cagtagattt 119820cagagttgct ctggttcaaa tgcctctgac aatgtcatct tagccctaat catagctgtg 119880acagactgcc actgctacta cttgagaccg tcactactgt tactgcctga gaccgtcatt 119940aaaagactga acaaagtgac gaacatagaa atgaaaactt aagacaaaag taactatttt 120000aaggaagggt ccaggggaag aagagagctc cctgcttcta gcgagcaaag gcagcccttt 120060gtatttattg ggtagaaaga gcagggagga ggaggtaacg actggtcagc tgcttaattg 120120atcacaggtt cacattatta ctaacaggct tcagatttgc ctaatcacaa gaaacacttg 120180tgcctggatt gtgactgccc tcctgtagtc cttctgggtg gcatacacag tttgtcagtt 120240cgccaacatt ctgcatttat gagaaacagt ttgctgttta ctcatatagc ctccagtggt 120300acactgagtt gatcatgact cttactcttt tggcctccaa caatagcaac tccatggcag 120360tctctatggt gttcagcttt ccactgcctt ccataagccc cagggagact tcagggaggc 120420tggaggagag tcagggaatc ataattggct ttctccttca tgtccacaca cgcatgcggt 120480gacacctcat gagcctgtgt actaaagagc ttgtgaccat ttcttcattc tctgtgtgtt 120540tctgttcttt ctcccgcttc tggattttga ctctgaggcc atcgtaatgg agaagtaagg 120600atgttatctc ctactcctct ttctatgctg tctcctcttc tagcactaga aggattttgt 120660cggcaaaggc ccttccttgc aaaggtttcc tcctgctgct gaagttggca ttagctctcc 120720catttcccta gggctgtcgt gatggaaggg tttccatgag agtggggagg ggatggaaga 120780caatattatc accaattcaa gaaaatgtat ttggaagttt ttgaagattt agagtacatt 120840tttgttgttt cactaaaata tctctttaat ttttctaaat tgtgtaactt tattcttaac 120900tgtttttgga aaatacatct ttttcttatt tttttccttt aaattatcaa ctaagatgtg 120960agttcttggg ctttgcttat tgacttaaaa ttttttttta attttatttc tagagacagg 121020gtctcactgt gttgttcagg ctggagtgca gtggtacaat catagttcac tgcatcttct 121080aactcctggg cacaagggat cctctcatcc tcccacctca gcctcctgag tagctgggac 121140tataggctca caccagcaca cctgactaat ttaaaacttt cttttttggt agggacagat 121200cttgctgtgt tgcccaggct ggtctcaaat tcctggcctc aagtgatcct cccaccttgg 121260ccttgcagag ttacccttat tgttttatta gtagagcaat tttgaatagt tagaataatt 121320ttgttaattt aatgaatttt tttttttttt ttgaggcaga gtctcagtct actgcccagg 121380ctggagtgca gtggtatgat cttggctcac tgcaatctcc atctctctgg ttcaagcgat 121440tcttgtgcct cagccttcca agtagctggg attacaggtg tgtgccacca cacttgggta 121500atttttgtat ttttagtaga gacagggttt caccacgttg gtcaggctgg tctcaaaccc 121560ctgacctcag gtaatccaca catattggcc tccaaaggtg ctgggattac aggcatgagc 121620caccacaccc agccttaatg aactttaata atgttactat ttgtgtcgat tagcaacata 121680gcagatagtc acaacagcta aaccatacag aacattttct atactcaacc caaaggttgt 121740gtgtaccgtt cgtcttccgg ccagttcagg ctttccatgg caataaatcc ctggggacta 121800ggacctttca ggaagcagcc tctggttaaa gtctacttcc agggatatgg ctgtttcctc 121860atggttctga gagccaggag gctctacgtg tggaacaaac tgaccaaatt caatgaatca 121920ggaagtgaca aagatagttc agggaaggat tagtctctag attactgtat ttttgatacc 121980aataattgtt cttccttttg cttattttac ctttatagag ctgggagcac ataaaagata 122040aggcaggatg tgaatttgac caggtgtgcg tggttatcac caggcttaaa gaaaaaaata 122100tctccctttg acatataaag agcttctaaa tcagacagca aatcataact agtgtcactc 122160agtgtcaggc attgtgctgg gttctttaga gttacagaac atttatgata cagtctgtga 122220tttcaaagag cttgagtctt gttgaaaata ttatccattc attcgctaaa tatttaatga 122280gttcatacta taggagaggc actgtgatat gtgggcatag agcagaaaca aggtagattt 122340gaaccctgcc tcaatggaac tgataatcaa gtggtactga tattcaataa ctgactctgg 122400atgtttccaa atatccccaa ttctagccat agaaagttcc gttgtttttt ttttcagatt 122460aattaaaaaa ctcaagcaca tcctgggtga cttcatgaaa tgcagtccct ctcgagcact 122520ctgatgattc cagggagtca ttaggttctg gctgtcacct gacaggtctc tctctgggct 122580atggttttgc atttccacag ttcccttcag gctctctctg atctgtttct ctaggtggct 122640gtcctctctc tactgtgctt ctaatcagtc tggggagcct ccttttttta ttcttcaaag 122700cttcaccctc ataaagctcc ttattcactt agttgtttag cctcttttta gaaatttgtg 122760atttacctta tttttaattg acaaataatc actgtatatg ttctgtattt atggggtatg 122820ttgtgatgtg ttgatatatg tttacactgt gcaatgatta aattaagcca attaacaact 122880ctatcaccta tttatgagtc tttaccgaga acttcaactg agccaagggt gaatgaggaa 122940ggagaaaatg aaaaaacagt gaaatttaaa aagcagagat aactactcct aatgtttatt 123000gactgctccc ttgcatagat gtccacaaat ggacagtaaa ttttttagct ctgcaaatat 123060ggagtgggag acagagagga atgatggtgg aaacatgaca ggagacttgt ggcccatgaa 123120ataaatgttt gtctttattt taaggctaat agaaaaataa gaagcaattt tacaaaacat 123180gtatgttggg atgtctgagt atgtatgtgt gtaatgagca ggctctcttt agaaacaatt 123240gtgtggagaa tggcttagct accaatatta ggtggcatac actagatgct ggatgagcag 123300gagcagtgtc atatactgat cgatgttttc cctcccaaat ctgacagtag atctgtcaga 123360catcaggaat ttcttcccaa cagcctgggg ggtggaatac tgcagtccaa tcacaaatga 123420tattcccaaa gtgagaggca gcaggtgtaa gactgagctt atttatatag caggtctgtg 123480tgattacaca cagtattgta aaatatacct ggtagaataa tttcatggag aaaaagtccc 123540tcttaacctg gtatcagaag tagatgtaca gggaagtgaa tgaagttgaa gtttcaaagt 123600ctctcaattt cacaggcctt ttcctaggca cctagcttta tattcatttt attaaagaaa 123660gttttgtact cttcagatgc cacaaaacct ggatccacct ctgtccagta tagtccccag 123720gtattcatgg agaaagtgga atttgaactt gcccttgaaa gaaaattagt ctgtgcatat 123780gtcaaaaaga atgggagccc attctgagtg aggataactg tgggggcagc atggagggtg 123840cactggaggg ccatgagtca attgattgat gggtaccagg gatcactaaa gcaagtgttg 123900gatacccaaa ctcatcatct aaggtgtttg cattggttgg ttatttacgt tccttggacc 123960ataggaaata gatcaaaact tatgttagct taagcaagag gaggtttatt gtaatagaaa 124020cagcattatg ggccatgcac agtggctcat gcctgtaatc ccaacacttt gggaggttga 124080ggtgggagga tcacttgagc tcaggagtta gagaccagcc taaacaacat agtgagatcc 124140tatctctaca aaaaataaaa acattagcag ggtgtggtga tgcatgcctg tagtcccagc 124200tacttggaag gctgaggtgg gaggcttgag cccaggatgt caggttgtaa tgagctgtga 124260ttgtgccact gcgctccagc ctaggtgaca gagacagact ctgtctgaaa gaaaagaaag 124320aaggaaggaa ggaaggaagg aaggaaagaa agaaagaaag aaagaaagaa agaaagaaag 124380aaagaaagaa agaaagaaag aaagaaagaa agaaagaaga aaggaaggaa ggaaggaagg 124440aaggaagaga gagagagaaa agaaaaggaa aaagaaaaga aaaaaaagga gaaagaaaaa 124500gaaacaatag tatggcttgg agtgtgtagg ctggagttgg agagtagatt aagatcctga 124560aaatcaacaa ctggagtaaa tggaatctct tgcctattgt ccaccattat tggacccagt 124620tattttctac ttgtcataat tttgtttcta ttaacagttg cctattcttt ctctgtattg 124680tcaatataat tccttagctt aatcatcttc ttcctcatgg ctctgttcct gatactaatc 124740ttccttattt tcttgacatt ctcaaaaaag gaagtctctt ataaggtcac tgttccttga 124800ctggcaaagt catagatcag tggctaacct attgcttgag cttgcacacc ttgtccaatc 124860aggtatgcct aaaatgtaca gaatctttgc acaaaatagg cgacctgtag ttgccccttc 124920agaagttgtg ggccagggaa tttcccttac aagaacctct aggtgaggct agcataataa 124980atggcctgtt tgttatggct gaaataggaa tagctaatat tttctgagtg cttactttgt 125040gtcattgcat cagattctgt tctaagtgtt ttatatacat taaccccttt tttaaaaatt 125100taagagtcag ggtcttgatc tgtcacccag gctggagtgc attggtgcca tcatggctca 125160gattcctggg atccctcaaa ttcctaggct ccaggaatcc tcctgcctag gtctcccaaa 125220gcactgggat tacagacata atgggcagcc tgcattaatc ctttaattcc accaacaatc 125280ttataggggt agttacaacc tttattccca ttccaccaag aaggaaactg agggccagca 125340agttgcagta acttgcccaa ggtcacgcag ctaataactg gtggaaccag gattccagct 125400gggcaatatt tctagagttt acccttcttc ctgcctaagg tttttggcta tcaatccccc 125460caaactatgt cgaagtcccc acttatttga aagtggtaac agttctattt ctccaattcc 125520catccacccc tatccaatct tagtccaaaa gtgaaacatc cacaataact agctgtattt 125580gctctctgcc ttccactctt gctcattcag tcctgtcttt gttcaagact tccccgcctc 125640ccttccagtt cttcttttca tgctctgcac ttccttatga aacctcaact cccctcaatt 125700actttaatat ttcccctttc tgtgaattct taaggcttct acaatctgtg ctattgtgaa 125760gagaccagta taactgcatt taaggtctat tatcttaaga atatattttg atgttgtaga 125820aagagaaaaa cacagaaccc ccttaaaaga tgaagattca agaagcagtg ctagaagtta 125880caaagcctag taactgtata atctctttag atagtgaggc tgtaaaaact ccaaaagatg 125940aaattaaatt gaggagacag ttgtaaggca ttggagtctt taatatagtt tgagttgatc 126000agagtctcac gaagtggtgt gagaacgctc aatggaagga cggtccccag cctgcccact 126060ttgtttctcc aggcagtttg aattctgaga ggaaagataa gaaaggaagg aaactttgga 126120aagtaagaag gggaaaccgt ttatttttgt tgttagtgtg atatagcagg ccccggacta 126180gaaatgatgg ctcacaccca aaagaccagc aagtaaataa tgacagtaga taacgtgcag 126240ctgtacccat ttcctcagaa ttttgctgaa gtgacaaatc tgtctgtatt ctctgtacag 126300aataacaatt aattttttta tgaaagcaat ctttttacag ctaaataatg aattaggcac 126360cagaagcctg gtttctagtt ctggttctag gtctagattt gagcaagtca acttcactca 126420gctttttttt ttttcaccta taaaatagaa ataatatcta ctcacttata gacattgcat 126480ataaaaaatg tagaagaaat cattcattca aacaccacat agtaattaaa ccacgttgtg 126540gctgactctg gtttactcac tgagggtcca tcaacaaaca gaaacgtgaa aatctctgct 126600cttgtggggc tcactgagta gtataattgg ctagctcaca tgttttagtc ttttaaccct 126660gctcagttat ctatactttc atttctttaa gaaacaagca tttgatgaat aaggtatgtt 126720ctagggtatt tatgcgtgga ttaaatctac aaatctatta ttgggcctag tgatagagca 126780gaaagagaaa gctagattcc agataggact ttgctgcaag ctagctttgt gactgtaaaa 126840gaaagtggct tcatatcctc atctgtaaat aaaagagttg gattacatca tctccaaggc 126900tccttttagc tttaaaatgt gcagttttct ttatgcccac agggatcact accctcttgg 126960tggcagctgg catggtgcct aagaagaact tgccagtgct cagaatatac cctgcaaatt 127020ctaacaaagt agatgatggt gaatatcacc atcctatgct ctggagagtg actatgtgcc 127080attttctact atttcttgtt atttcaattg cttcccaact agagcacaca ctcctgcaag 127140gcaggaatca ataagtgcat tatacatatt attatccgga tcaggaaaga ctcctctgat 127200atcagaagcc tggatttgtg tttctaaaat tatctatatt ttcccccttg agtaggtcaa 127260atgctgctcc tattacctta ttaatttttt gttttgctta ttaatttgac caattaagac 127320tattttatgt cacgaacaat ataaaattta gttctaccct tattagaata atgtgttcat 127380tccagcttaa tgaagtttca ttatacttgc ataaaaagtg gggggaggtt tgtaaatact 127440agaacttaat atgcaataca ggtatatcta gcctgagtac tacaatcaaa gatgagagca 127500tataccaaaa gtttctaaga gactagtttt attagcacag gaatgaaaac caattataga 127560atttggaggg agcgaggcag tagagtgtgg ttaggacatg tgttttggaa ctagactgct 127620ggcatttgaa ttcaaactct gccactcatc tggaggtgac cttaggcaag ttactttacc 127680tatctatgct tcagtttctg catatctcaa gtgaagatga taagagtacc gattacaaat 127740aattaaatga attattattt gtaaagtgtt tgaacagttt ctgacaaagg gagaggacct 127800tctaattctg gtcagttgtt taggagggag ctaaaaggag aggaattagg agcacctgag 127860actgcatgtc acttaaagaa gctggtgtta aagggaggca aaacattcga ctgcacttgg 127920aggaaggagt agagacaaac agtcaagcac aggtgtttgt gtgtattggt ttcccaagac 127980aagggaaact tccacatatc tgaaagtgga tggaaaggag ccggtggtca ggaagttgga 128040agagggaggg gatagctatg ggagcgggat gacctgtaac aaggactcaa gtaatcagag 128100gtgaagagag ggaattctag gatggttttc tctttttcct tcaaaatcta gctagattta 128160tgccaagtta cacttctggc cttgaaattc tgaaggtgac agcacagacc atcatagtcc 128220tcagttattc aggatcaagc agtggatggg gataaattag tgagctagtg taggtgagca 128280gaaagaacta ttaatgacat gatggaaagt agctataacc ctgtggtaca gctgggtcat 128340tctggaagga atctcgtaga caaactagca tagtgttcag cccacagagg cagcatggct 128400ctgtgaatac aggcatcagg catcctcagc taaaccataa atggcacagg atccacaaat 128460gagccatagc agaaggccaa acaaatgagc tatgtttctg gagtctccat ttggtgtctg 128520tgacgtgccc atttacacat caatcttctt tcagaattgc cataggaaaa taatttggcc 128580caagagttaa attcaaattg gaagatcaac accgaccctc cctccaaata cgcagttaaa 128640tagcctgaac caccttaact gaattatttt aaaatatcct gtaacacagc ttagtaaata 128700aagctgtcta aattgaaatc tttggggtct cacatccaaa gggaggataa agtttgctgg 128760cagattggat atcttatata cttggcaatt tgggaagaac acagcagagc tgcaagttta 128820gttgaaacaa gaacaagatt tatttcagac gggaagatcc taggcagcat aaaggaaaat 128880ctttatggct gcaagagctg atatgtctaa gagaggctcg ttagtacctg cttatcctta 128940tactttcagg acaggtcata tttgatgtga atgaagatca gtgtattaaa gggtttaagc 129000atgaaatgaa attaaaggca aaggagcttg gcatcacctg acatatggta gtctttatct 129060tttagaaatg ggaaactttc aaagatattt atgatttgcc aagatttgcc ttggaaacaa 129120tatgcaggat atgttaaaga gaaggaaaac tttggtcaga aacagtagta acaaggctat 129180tagtagatca aatcatatat aatccccaat atacagcaat tttttaaaat ggaatttatt 129240ttttggagca gtccatagca gaattgagaa gtgcagagat ttttcacaga ctcccctggc 129300cccacatatg catagcctcc tccattatca atgtccccta ccagagtggt atgtttattg 129360caattgatga atctacaagg acaccgcatt attacccaaa gtccatagtt tacattaggg 129420ttcactcttg ctgttgtaca ttccatgggt ttggaaaaat gtataatgac gcgtccacta 129480ttatggaatc atagattagt ttcacagccc taaaaatcct ctgtgctcca cagacagtgc 129540cactagggaa acatatgctg cagaactctt ggctcttgac aaactacctt gcagtgcctc 129600ttactttagg tttttttttt ttaatctcct gctatttgac attgtacagg tatgtgatat 129660cgaaattcct acagctgtag tctgcttcta aagtttagcc aacaaataat gcaagcccaa 129720gggcatttcc ttagttaaag caggcctcta aataagaacc aagcagcata atagcaggaa 129780gaggaaccta gttgtcagct aaggtaagaa cttatcctaa ccagatcgtt ctttagcaga 129840tggagaatct tctcacatag gagtacaaag ggggctttga aagtcttgca gacaaaaaat 129900ttaaaaagac cccaaaaacc tgttctcctt tctcctccaa gtctgaccac tcttccagaa 129960ccataaaatt tctgtgcaaa ttgccactaa actttttctg tttgctgtgg aataggtatt 130020ggtcaaagtg taatttttgc tgcaaagaaa acatggcatt ttatgattct tcacacagca 130080ctgcctacct aaaacagaaa tcatccattt gcttaacaca gttaaagatt ggaaaactat 130140atgtctgtct ggactgcttg tataatgcca tgccacttaa aatggccgtg ctgtttaaga 130200taatcttaat taaattttta aggaaaaaaa aactttagaa agccaattaa aaaagactga 130260agaaacactg gattttattt ctttctcctt tctcttgtaa caaattttta tgaggaattc 130320attttattgt gtctttttct tataagacaa ttactgagaa atttataaaa aaaaggaatc 130380gtttttctgc tgtgacatcc aatgaattta ggagatgtgt aatatggaga atactgttga 130440gggatgggcc tcaggactgg ctgtgcagca ttgtccactc aggccacaaa tgcttatcca 130500ctatgatttc tgtgccaggc accgtggtag ctgctggaga ctcagtggta agcaaaacca 130560ggcacagttc ctgctcttgt gaagcttata gcttaggtac aatgacacat caataaataa 130620tcacacacat aactatacgt ttcatatgcc atgagttcct gaaagaaaaa atagtagatg 130680tcatatagtg aggcaccagt cctagtgaac aggatgttct ccaagaaagt gattctccaa 130740gttgagatct atcagatgag tagaagttag ccaggtgatg aagaaatgga agcatactgc 130800aggcagaaga aagtgcaagt acaaaggccc tgaggtatga agaaggatga cactgttcat 130860aatgttcgaa gatggctagt gtagcctgag catttaagat caaaaaggca aaaagtttga 130920aaagagtttg aaattgcatc ccgaggccag ataaaacagc ctttgatgtc atgttaggaa 130980ttctattctt tatcccagag caatgggaaa tcacgaaaaa taatcagctg gagggtggtt 131040gaattagatt tcccttcacc aatcacttga ggtcaatgag ttcttattca gcctttaaca 131100agtgctgtga taacttcccc agtcatatca gacatggtct ctgcctgcaa gtcgtatttg 131160atgcgtaagt gtaggaggaa ataagtgtga gtcaaagcat ggaagtagaa atgagatggt 131220cctaggaagg taatatctaa aacatccata ttgatcatag ccaatgtgac tttgatatgc 131280aggttttggt tttgcttttc tttaaaaata ctcaaggaag tgtcctgtga aatcctctgt 131340ctgttcacag atcaggtacc acacaaacaa ctatgcatcc agctccacct ccttaccctg 131400ccagtgttcc tcaaccttga tgtggagcga ccatttggaa aggtgagatt tagaaactgc 131460cgggatttat agcatagcct tcccccgtct ctctgttcta atcggagttg atgctacaaa 131520ggcagaccat ctctgagaat taaaatgcaa attaaacata tacatctgat taagagtgct 131580agattcctta gaggagttgt tgtttttaac tttacttaag tttcctccaa ccaagagttt 131640tttgaaactg gcttgagtct gatcttgtga ctgatcaagt ctcttgccat gaaagaatct 131700gtaagtcact gattttttca tagtaaggac agtttccagt agtatgttaa ggctaggcca 131760ctcctatttt aattaggtat cagaatccag cgtgttcgcc atatggctca tatggctagc 131820acactctcag tcgcattttt gattagatct attttgaata aaacatgtac ttcaatgaat 131880gagtactcta aaaggcagaa cttaatccca aatgtcatgt atgattggcc atatcaaatt 131940ttgagcttta gtttcttccc tcaagcacct ctttttagta tttctattaa tggctaattg 132000aatggaaagt ctgctgggaa ttatagcatt gtgtgatttt tttcttttta agaaggactg 132060tgaaaaggtt tattttttca gagataacac agttgactta tcaaatgagt tgaactcttc 132120attttagcga aaaagcatgc tcattttatt ttattctctg ggtaatgtca ggtacagtaa 132180cccctctgct cggtacaagt tgttataact gatatgaaca ttcaagcagc tcaatttctt 132240tagacattta tcctcatggt tgagggaact gagagaattt ttcctgtttt tcagaggaag 132300agatgaatgt gttttttcaa tagagatata tagtgtgcca cttacaggat aagaacaagc 132360tggttgattc tggaaaacag cttaaaaatc atgaggccaa tacaattttt actcaactca 132420catttataat cctatatcct tccccacatc tatctgtcta cgtatctatc atctatctat 132480ctatctatct atctatctat ctatctatct atctatcatc tatctatcta tctatcatct 132540atctatatct atgtatctat ctgtctatct atctatgtct ataacacaca cacacacaca 132600cacacacaca cacacacgtg cacactgggt actgttttgc tgttaataca gatttcccta 132660gaaaaccaat gaagggaaac gaagagagat aacttgtttc catctctttt ttggtaggtt 132720cactctaact ccatttttaa ttttttaaag taaatcttat tatacttttg ctcagttaca 132780aaaataagcc ctgttctcta tggaaacttt ggaaaatgat gaaaattaga aagaaaaata 132840gcttactaaa ctttagtctc accagtcagt aaaaattcag tatcatgtta gtgtatgttt 132900ttctattctt tttttcctgc agttttttaa ttttgtttta catacttatg ttaatattat 132960gatatacatt ttttaatctt gttctcattt tatttagcat aaaataagca tttctcagac 133020catttgcaaa ttctttgtga agccatttta atatttatgt aataggttat ctaaataata 133080cactgtagct tacttaacca tatggtcaat tttgtggtca aatttgccac attgatgaaa 133140atattactgt atcattaccc aagtactgaa aggcagaggt ttgaaaaaac tgtttatata 133200tatatatatt ttaccataag gagaatttat ggaataagta atgtgtaata tttaatgttc 133260tttaaagata catgtagaca gactgtttgc gaaaataatt agatttaaaa tttcatcaac 133320cgtgtgtgaa aaaatttgct ttatactaat tggcactgtg tactatctta aaaaaataaa 133380cagaatactt taattaatga attaattatg tttttttgag atggagtctc gctctgtcaa 133440caggctggag tgcagtggca tgatctcggc tcactgcaac ctccacctcc cagattcaag 133500cgattctcct gcctcagcct cccaagtagc tgggactaca ggtgcacacc accacaccca 133560gctaattttt gtattagtag agatggggtt tcaccatgtt gaccaggatg gtcttgatct 133620cttgacctca tgatccactc gcctcggcct cccaaaatgc tgggattaca ggcatgaacc 133680accgcgtctg gcctactttg atgttttgat aggaaaatat tctatttcct ggctatttca 133740gcttctattc ttccttattt ctttttagac tagagttata tactaattga aacttgtttt 133800tacaacaaga ttatttgacc atttattgtg cctacaattg cttttttaaa tctaaaacac 133860atgtaattac ttcaaaagag aatgttttaa acgtttaaag caaacaaatt tattacttat 133920attttactcc tcacaccata cacaaaattt aaaaaaaata atccccatcc atgcttattg 133980catctttctc tctctattct aacagataat gaaagaacgg agttggacat tttattgcaa 134040ttcctgcttc cctgaatttg catatttctt cccacctgag aaggataatt atatatttta 134100atttggatta tttcttcatt tttatctttt tattttaatg attgttttgt cagtaatacc 134160catggagatc aactttatta ttataatcca tgcctctgaa tattagattg gtttcttgga 134220tgggattttg aatatgcatt taagaagttg ggaagaattt cacagatgat gattggagga 134280aaagtgatga aaagaaagac ctgtgttcca ggagttttct ccaacttcaa acctttacgt 134340gaatcttaac caaagtggac atctttacat ttcatgatag cttgcttttg caatatgagt 134400ttgaaaaatc agtataagct tatgatggtg aaaagtcaac atattgagag tgataattca 134460attaatagga tatgaactta acgacatata aaagcaaatg agggcaggag ggaatcgtga 134520caaaaaatat ttgtgcccaa ttatgattaa tgttttagag atgttgtgtc cctgtgggct 134580tagcatggaa ctaaaagttc ctaagtctca attctagtta tgtgtcattt agtaactcag 134640gaatctgctt aattgttatt tctaagcttt tgatgacaaa ggagtgatgc agctaagggc 134700atccttggag tgtcataaaa aacaaatttg aggttgaatg attagctgcg cagttagagt 134760gataaaaaaa tccaggtagg ccttctgatt caccatgacc aagtcaggat ttcttaatat 134820ttcttttctg gcagcatata caaaggcaaa attaataaat aacagttgtt gaataacaaa 134880ctttattacg tttttataaa ataaaagaat ctattttgtc tgtattaaaa taaaaaactt 134940tgtggacttc taaaagatat acttgagtca tctaatagtt ttcaacggat atgccgttac 135000ctgcttttaa attcaatcag cttttcttct tcttgtcctt ttttttctct tcctcccttt 135060cttctgtccc ctcatttgct taattcacca catatttact gagtgtctcc tctttaggta 135120ccatgctaaa ggccatgctg tcacctatag gacatcttgg gaagcgatga aaatatggta 135180tcagaagttt tgctgtagga tcatatgctg tgactacata tcgtttctgc caactataat 135240gactcaatag tagagtcctt cttaaatagc agaagcagtt gtgaattagc tataaactat 135300aactattttc ttccaaatct atatttaaaa tggtcttaac tagcacaaag ctgatgctgt 135360acacaatccc tttacttagg tttaaattca gtgaatttta ttgagtacat agtatttgcc 135420aacatcagaa gaatgtccat tcttctgtta cttctatctg ttcctcattc acctgtgtga 135480ctagctgtca tctattcact attttatttt tacaaaagat ggtacagttt ataaagggct 135540taaccgattt ttcataccta acataagtaa gaattcaaat tcaggttcaa gtttttaaaa 135600ttccaggtgt actgttcctt cttacagcac aatgggatga aagatttgct gcagtgatat 135660tctgtttaca agacaaagtg atgtggtaac agaagcatga gtttggaatc aaacttggat 135720ttaaatctgg ctgtgccact cacaagctct gtgactctga gaaagttaat tgcatctcaa 135780taaattaatc tataaattga agataatagt acttacctgt agggtaattg taataacgta 135840cataaaatga gtgttacacg atagatcctc agaaagtatt tactatgatt gattctttac 135900ccagacttat ttctacctat ttaatgtgaa tctcaatttc aactataaac cggcttcttg 135960actctgtggg tttaaatgtc atgaacggaa tcaaccccaa ccatcctttg gatgctattc 136020ttgtttttaa gacctcacga taatttttgt ccatgcaaaa cctacccacc tcccacatgt 136080tcagatctca ccttgtcctt aggccttgaa tactttaagt atttttgttt tttccttacc 136140tctgtctgca actttgaagt tacatagtac tcattttgac tattgtattg tactattcct 136200tgaatatttt cattgtatat gtctcttccc atgtgacaaa actgtaattc atggtggaga 136260ggactcgttt cttaattttt ttccacaggt ctcttggcac tagtgtagtg tttgacacct 136320agaaagatgt tactaattaa gtaaattatc agcgaaggta acatttatgt acaagattaa 136380cataagtgaa catgatagct ttttgttttt atgttgttgt tcatgttagt ggttgttgtc 136440atttcacgtt aaactttcct tgtttaaaaa tgtctacatc cagggagaat aatcacaaga 136500agtgcttttt gtttgtttat ttgtttgttt ttaagacaga gtctcactct gttgcccagg 136560ctggagtgca atggtgcgat ctcgggtcac tgcaactttg gcctccccag ttcaagcaat 136620tctcctgctt cagcgtcctg agtagctggg attacaggca cccaccacca ctcccagcta 136680atttttgtac ttttagtaga gacggagttt caccatgttg gccaggctgg tcttgaactc 136740ctaaccttaa gtgatccact cgcctcaacc ccccaaagtg ctgggattac aggtgtgagc 136800caccatgctc agcccacatg aagttttaca tagcatgttt attaaaggtt tttttgttaa 136860agtaataagt aacaaaataa ttagaaaagt tagaaaaata atgactattt ggcaaagata 136920cttctcatac aatgagaaat atctttgtcc aattagttta aaatctgatg tagagaaact 136980gtatatctat atatacatat cagtatatat atatatgata cttaaaaaac tactttagtt 137040tttagagtag ttttagtttc acagaaaaat tgagcaggta cagagaacat ttttgtttac 137100aatctagaca aatgtatcca ttgtgtacaa attcattgaa aataatgtta tattatgtta 137160tatgtgtact caaatactct gggttgtaat tcagcaaaac actggttttt aacaagtagc 137220ttcatcttca tttttgttat tttcaataaa cacaaattct tgtcattatg caacaaggtt 137280ataataaaga tagaactaac cctcaaaata aagtaagatc cttaagaact ttgccaaaaa 137340aaagttactt gtgaagaact gatgtttgat ctacagtttt attttaatta tagtaactac 137400tgtaaagttc tgtgggatac atatgtgaaa caaacatata acattaatat gctttaagtg 137460ggctgtatgg acatgagtcc tgactataca tataatatat tccacgtact tttaaactta 137520cagcttttgc attggaagaa atgaactgct gcctactcca gcccccattg tattgcagca 137580ttagtgcttt cattcagcat cagtgcaacc caatgtaaaa gaaaataaac aaacaaataa 137640acaaaaaact cagctgtgct gttcccaaga gaggttggac cagggtaatg tgaacaagca 137700tgccaagcca tgccgagatc tgttagccag ggatacgtaa gagaacagag aatattgtgt 137760ttagaagctt tggcttccgt cagaaggtat atttatctaa gttctcccaa ggagggaaga 137820gaaaagtaga gtcattatct tttacataat aaaatatatc cacagaatat atgaaaattc 137880agacagagtt gacttgggtc tgtgttttta gtatctaaaa gaacaatttt caaaaattaa 137940ataaataatg aaataaaagc tataaggaaa tcagataaac taagagaaaa agtttttcaa 138000catcctttgt aattggaagc acatttcaaa gaaaactgtt cttaagataa ttgtcattat 138060gcaggtatct taaggatctg tataaaatat acgcttatca ttatattttt agttatgact 138120acagaagtgt ttaattgcca tactaaaaat atctgtcctc atatttttca ttaatccagg 138180cttttactga ctattttatt aatacaaaat atttctaggc tcatcctaga ttttcgacag 138240ctttattttc attatctgaa ttttttttac tttaaggtaa tttgtacaag atatattttt 138300aaaaacttct gtatttttat ttaaatctca accacattgt aagctattgt gggcaggact 138360taaagcacct acagagaacc tatagagcct agcagagtca gtactaagca catagaaatt 138420gctcaataaa aatgtgatga atagtctcgg tggaccagac tataatataa atgaatgatg 138480cctaagacaa gtaggtgata tcacactgtg agtaatggtg gcatttagtt ggcagttgat 138540tctctattta catttctttt ttgatattat gccattttgg cagtcttatt taagtagtac 138600aaggaagtct ttccttagac ttcttgaaga aaacatctgt atttttttta acatggcatg 138660acatgactgc tgttaagaaa aaatgtaaat tagttcaatc attgtggaag acagtataga 138720gattcctcaa ggatctagaa ccagaaatac catttgaccc agtaatccta ttactgggta 138780tataccccca aaatagaaat cattctacta taaagacaca tgaacatgta tgtttattgc 138840agcactattt aaaatagcaa agacatggaa ccaacacaaa tgcccatcag tgatagactg 138900gataaagaaa atgtggttca tatacaccat ggaatactat gcagtcataa aagggaatga 138960gatcatgtcc tttgcaggga catgaatgaa gctggaagcc accatcctca gcaaactaac 139020acaggaacag aaaaccaaac accacatgtt ctcattcata agtgggagct gaacattgag 139080aacacatgga cacagagaag gaaacaacac acaccagggc ctgttggggg gtcgggggtg 139140aggggaggga acttagagga tgggtcaata ggtgcagcaa accaccatag cacatgtaca 139200cttatgtaac aaacctgcac gttttgtgca tgcatcctgt tttttttaga ataaagaaaa 139260aatgtaaaca atgaaaaact agataaatat aagcctgcat attaaataac ttcttttttt 139320atattcattt tttaaaattt tttatttcta taggttttgg gggaacaagt gttatttggt 139380tacatgagta agttctttag tggtgatttg tgagattttg gtgaacccat cacctgagca 139440gtatacactg aaataacttg ttttagccag ctatctaatt tcttaccttg acagaagagc 139500tgaattttaa ctttacacct acaggcagat gaaggtattg cagaagatat atagcctgta 139560caataaaatg aacataattt gcacacatga gaacaggaga aaatataaat tgtattactt 139620ctttgagcaa agtatagtgt tctctttaca gaaataagta caccagagcc ttagtaagtg 139680aataatagtt ttcagtttat ttccagataa aagttggtga ttgctaatta tggctttaat 139740cagtttattg gtttaacata aaactcaaat gctaaatctc tttagatgca ttggaattac 139800ataagaaata tttgtggagc attactttga gaaagtagta aaaaataaaa ttccaagtac 139860atatcaaaaa cctgagaact taagtaacaa gctctgaaat cctatggtcc tggaagagca 139920gaagaaaata cacgctaaac cacagaacaa tgaaaaatac aggataaagc aaaagtaaag 139980cagagaggaa tgtaaatttg tcacaaaaca agacaaagga taatttatta agaggtgctc 140040cataaacact tgctgcttgt tgtggtggtg gtagggagat gctgctgctt gatggtgttg 140100gtaaagaata agacataggt attcaagtaa aattagctaa ctgtcatatt aagtatggag 140160ggaatgggga aaagatagta atatagtctt aaaaaaactt ttgaaaaagg aagaaaaata 140220aaggattgag ttagtctcct ttctatgtat ctaatcatcc tgtaagttac attagttcta 140280caagtattac tcaattctgc ccctccgtct ttgctggcat ggtctacatc taggtctcca 140340tcatttccca tctgcattgc ttcagtaaca ggtgtctttc ttctggtcct ggcccctcca 140400tccattttcc acacctgctg ccagagttat gttttgttaa taaaatgaaa atctgctttt 140460ttttcttttt taaacatctt taatattttc ctgtgacttt taggataaag ttaaaccaac 140520taaacttagt ccacagagct ttctacaacc tagttcctgc aaggctctga ttttttttac 140580atccacctgg atttacagca atgcctacct gctctctcac atctctgtgc ttttcatatg 140640ctgttctatc tacattggat actcccttct ccctttctgt atgtgactaa ccctcactca 140700tctttcatat ttcaacagaa gtaccacttc cttcaagaaa cctccttcct ttagaaaacg 140760tttttctgtg tttaatttgt tggtttattt gtccatcttc tttgctatcc aagatgggaa 140820ccatatttct cacttccacg tctggtgtgt ctatcacggt gtatggcata gagctggtac 140880aaacaaattc ttgctggaga aagaaagaac ggaaagaaga tatgcaacaa tcaactatga 140940aagtttgagc aaacataata attcaagata attcaagact gaaaccatcc ttttgttact 141000taaaaccaat agttagtcac attttctgaa taaccgcttg atatggtttg gctctgtccc 141060tgcccaaatc tcatcttgaa ttgtagctcc cataattccc acctgtcatg ggagggaccc 141120actgggaagt aattgaatca tggggcgggt ctttcctgtc tgttctcatg atagtgaata 141180catctcacaa gagatgatgg ttttataaag gggagttccc ctgcacatgc tgtctttcct 141240gctgccatgt aagacatgac tttgctcctc attcacctcc tgccatgatt gtgaggcctc 141300ctcagctatg tggagctgtg agtcaattga acctctttcc tttatagatt gcccgtttta 141360gctatgtttt tgttggcagc atggaaacag actgatacag taaattgagt gttgctataa 141420agggcacctg agaatgtgga aacaagtttg gaactgggtg ataggtagag gttgaaatag 141480tgtgcagggc tcagaagata ggaaaatgtg ggaaagtttg gaacttccta gagaattgga 141540gggctcagag gacagaaaga tgcgggaaag tatggaactt cctagagact tgttgaatgg 141600cttggagcaa aatgctgata gtgatgtgga caatgaaatc caggctgaca tggtctcaga 141660tggagataag gaacttgttg ggaactggaa caaaggtgac ttttgctgtg ctttagcaaa 141720gagactgacg gcttttagcc tctgccctag atatatgtgg aactttgaac ttgagagaga 141780tgctttggga tatctggtgg aagaaatttc taggtagcaa aatgttcaag aggaagtgaa 141840acattaaagt ttggaaaatt tgcagcctga ccatgcaata gaaaagaaaa acccattttc 141900tggggagaaa ttcaagcctg catcagaaat ttgcataagt aaccaggagc ccaatgtgaa 141960tcaccaagaa aatggaaaaa atgtctccag ggcatgttgg agaccttcct ggtagcccct 142020ccatcgcagg cttggaggct taggagagaa aaagtggatt cctgggtccc cctgctgtgt 142080gcaggccagg ggcttggcac cctgcagctg ctccagccat ggctagaggg agccaaggta 142140ccgttcaggc agtggcttcg gagggcgcag gccctgggcc ttggcagctt ccacacggtg 142200ttgggcctgc aggtacacaa aggccaggaa ttgaagtttg ggcccctctt cctggatttc 142260agaggatgtg tgcagatgcc tggatatcca gagattggct acagggtcag ggccctcatg 142320gagagcctct gctagggcag tacggagggg agatgtgagg ttggaacccc catgcagagt 142380ccccactgag acactgccta gtagagccat cagaggaggg ctcccatcct gcagacccca 142440gaatgtggat ctcccaaagg cttgtgcggt gtgcctggaa aagccttaga tactcaatgc 142500cagcccatga aatcagtcag gagaggggat gtaccctgca aagccagagg gacagagctg 142560tctcagatta tgagaaccca ccttttgcat tggcgtgacc tggatgtgag acatggagtc 142620aaaggagatc attttggagc tttaagcttt gactgcctca ctgaaattca taattgggtg 142680gggcctgcat ccccttcatt ttggccaatt gctaccatgt ggaatagctg tatttaccaa 142740atgcctgtac ccccattgta tctaggcagt aactaacttg gttttgattt tacaggcaag 142800ggacttgcct tgtctcagat gagactttgg actgcagact tttgagttaa tgctgaaatg 142860agttaagact ttgggggagt gttgggaagg catgattggt tttgaaatgt gaggacatag 142920atttgggagg ggtcaagagt ggaatgatat gatctggctg tgtccccacc caaatctcat 142980cttgaagtgt agctcccaga attcccacat gttgtgggag ggacctgagg ggaggtaatt 143040gaatcatggg gtcaggtctt tcctgttctg ttctcatcat ggtgaataag tctcatggga 143100tctgatggtt ttataaagac gagttccctt gcacacactc tcttggcctg ccgccatgta 143160agacatgact ttgctcctca ttcaccttcc accatgaagt gtgaggcttc cccagacata 143220tggaactgtg agtcaattga acgtcttttt tttttataga ttgcccagtc tcaggtatgt 143280ctttgttggc agtgagggaa cagactaata cactgcccag ttgatggctt tcctgtaaca 143340gacagagcta attttacatt tagaatcaaa gcacgcagag tcctgttgag atagcaaaca 143400aaactaagga tgattgttgt aaccttttct cacttggtag aatttaaaca caatcttgga 143460aactccagga aaaataacaa attaaaatgt tgctctcaaa cctgacccca catttcttca 143520aaagtagaat agagtcgtct tagaggagca gcttaggctt gtaagttgga tagctgtggg 143580tttgaatcct gactgatact aggcagttgt ataatcttca agactctata tctgatcttg 143640tttagcctca gttttcttat ttgtaaaata gagtaacatc tacttgcagt gctgctgcaa 143700aaattagagc taaactatat caggtcacta tatttagaat cattacaaat ccccaaaagg 143760caaagaaggt ccaaagaaag ccatttacag ggtgcctcaa ctttgagttt gtggctggaa 143820tttggatcta agagatagga attatatgtt ctcaaaagca gcagatgggc atgatagggc 143880cctaagcaat tttactacaa tccttttgga aatctctaat tgttctctaa agagttaact 143940tcaattatca ttagccatct gctgaaccaa tgggatttcc tctgcaagtg ccagcaagaa 144000tattcctgcc aaataaagag aaagtgcttt tgaaataaaa cttgtagagc ataactcaca 144060cgtaaatact ttcaaagtta agaggaaaca gcctgaggct agagccacca ttaatattca 144120gagcaaaata tgcatgttgt atgataagaa agagtgaaag ataagcagag aattaccagt 144180gaaggctaca tcatctgagt tggtttcaga gtggcctctg gatgacatca tgataccagg 144240gccagttggg attaaggaag actctctccc aaggcacatg ttcataaaaa gaaaaagcag 144300ggtactgtgg tcagccaatg ggatcaggaa attcaaggta cagaacctag tacttgtgct 144360actcttagct catcacatag ctactgccat attgcaaaat tcactgaaac caagtggaaa 144420aacaacagat gctaaaaaag ggaatttata gagatttggt gtagcatttt ccaaactcac 144480ctaatcatat taatcaggca ggattgttga aatagaaaat tttaggctct tccctggagg 144540ttgagtcaga aagtctggca tggggccgag gattttgcat ttttttaaac cttccaggtg 144600attcttctca tcaggcaggt ttgacattgt tacagtccag tggttcttaa agtattgccc 144660ccagatcagc agcatcagca ttacctggga actcattaaa aatgcaagtt attgggaccc 144720cactccagac ctatgaaatg gcaactatgg ggtgcagtgg gaagagagac agcaatctgt 144780tttagaagac ctctggtgat tctcatccat attaaagttt cagatctaca tttgtcaaat 144840gtggcccaga gaactagaag tggggagtgc agctgctgat taggaaattc tcctggccca 144900aattctccta ggactaagaa ggtcatgaat taaatccaga tactaatgtt gcttttccct 144960tgacttcatt atctcctttt ctatttttta aggagcagtt taattttgtg gcccttttta 145020actttcccac tgatcaaggc caatcccctt tctgcttgat agcaaggatt ctttattatt 145080ttcctccttg atcctcttgc ttctttgctc tcatcttaaa gacattgata gaatgacatt 145140ggtagtatgg tctctcattt ctcatatttc tgtcaaatgt gttttaatac tggaacgtat 145200ccagagatct gctataatat tttttaatgg caagttttag cagatcttgt cctccttacc 145260aagcctcact tttttttcac cttttggaag ggcaaggcaa tgcttaaaga tattatccat 145320ttttaaaatg gccatcattt tgaaggatag attctggcat caatgatatg caaatattcc 145380cactcaatgt acaaaagcag aaataataag ggagtcacta atcagctaat gagacaattt 145440tctggcacaa ctgtgaagaa agtgtattag tgttagtttc ttagggttga catcatgaaa 145500taccacatgc tgagtggttt aagcaacaga aatttattac ctcacagttc tggaggctgg 145560aagtccagga tcaagttgtt ggcactgttg gtttcttcca tgagctgtga gggaaaaacc 145620tgctccaccc ctttcttata gcttctgcca gcaagctttg gagtttcttg gtttgtagat 145680gcataccaat ctatgccttt aggttcacat agtgttctcc ctgaatgtgt gtgttcccac 145740tttttaaaga acaccagtta tattgattta ggggcccatc ctacctcatc ttaactaatt 145800ctatctacaa tgaccttatt ttcaaataag gtcgcattag gtaccaggaa ttaggacttc 145860aatatatgaa tttgaaaggg ggtgacaaaa tcaatttata acaaggaggg ttcatagagg 145920gaggaaacca aatctaaact ccaaaatcat aaagacctga catgatgttg tctgtgggac 145980ttaaagtgtt taaaaaattg aataaaaatc tcaatgacac gtcagccctg gctacaatta 146040agaaatctat aaaacataga tgaaaaaaat tgaagagggg ccgggcacag tggctcatgc 146100ctgtaatctc agcagtttgg gaagctgtgg cagacggatc attcgaggtc aggagttcaa 146160aaccagcctc gccaatatgg tgaaacccca tatctactaa aagtacaaaa attagtcaag 146220catggtggca gttgcctgta atcctagcta ctcggaaagc tgaggcagga gaatcataga 146280atcatttgag cagggaggtg gaggttgcag tgagctgaga tcttgccact gcactccagc 146340agcctgggca acagagtgag actccatctc aaaaaaaaaa aaaaaattga agaggacaca 146400aataaattga aagatatcct atgttcatgg attggagaaa acaatcatta aaatttccat 146460attaccccaa tctacagatt caatgcaatc tacagattca atgcaattct tatcaaaata 146520ctagtgacat tcctcacaga aatagaaaaa acaattttaa aatttatgtg gaaccacgga 146580agacccagat agccacagca attctgagca aaaaagacaa agctagatgc atcacactat 146640ctgacttcga aatatactac aaagctctag taaccaacac agaataatac tggcataaaa 146700atagacacat agatcaatgg aatagagaac caagaaataa attcatgcat ttacagccaa 146760ctgatttttg acgaagatgc caagaacaca cattggagaa aggacaatct cttcaataaa 146820tggtgctgaa aaaaatggat agccacatgc agaagcatga aactagaccc ccatctctca 146880cagtatacaa aaatcaactc taactggatt aaagactcaa acataagatc caaactatga 146940aactactaca gcaaaagata gaggaaatgc ttcatgaaat tgaactggga aaaggttttt 147000tgaataaaac ttcaaaagcc ctggcaacaa aagctaaaat agacaaatga gattacataa 147060aactaaaaag cttctgtaca gcaaaggaaa tgatcaatga agtgaaaaga aaatctacag 147120aatgagagaa atatttgcaa actatgcatc tgataataag ttaataccct gattatataa 147180aaaactcaac aactcaatgg ccaaaaccca aaaaatcaga taaaaaatgg caaaagacct 147240gaatagacat tttttcacaa gagtacatac aaataaccaa cagatagatt aaaaatgcaa 147300aacttcacta atcacctgag gaatgctcat caaaaccaca atggtatcac ctcactgcag 147360ttaaaacagc ttttttcaaa aagacaaaca tgctggcaaa ggaaagggaa ctcttacaca 147420cgattggtgg aaatataagg taatacagcc attatggaaa acagtataaa gtttccttta 147480aaaactagaa atagactacc atttgatcta gcaagctcat tactgggcat atatcaaaga 147540aaatgaaatc ggtatgttga tgatacatct gcacacccat gtttattata gtgctattca 147600cagtagtcaa gatatggaat gaacctagat gaccatctac agatgaatgg attaaaaatt 147660gttatacaca caaacacaca cacatgcaga cacacaatag aataccattt aaccataaca 147720cagaatgaaa tcatgtcagt tgaagcaacg tagatgaacc tggaggacat tatattatgt 147780gaaataagct agacacagaa agacaaactg cataatctca ctcatgtgaa atctaaaaac 147840attgatttca tagaaataga gagtaaaata gtggctacca aaggctggaa ggggggacaa 147900caaaaggttg gtcaattgct gcaacgttaa attatacagg aagaataagt tttggtgttg 147960tattacacaa tacagtgact atagaaaaat aacattgtac tacatattca agatagctag 148020aagagaagat tttgaaggtt gtcaccacaa agaaatgtct aaaatgatgg atatgataac 148080tactccaatt tgatcattac actatgtaca tgtgcctcaa aacatcacat tgtaccccag 148140aaatatgtaa tattattgtg tcaattataa atttaaaaat aaattttaaa aaggaagaaa 148200attcaaaagt ggtcagattt ctgaccctgt attatctgga agttttccct gcaaaaaaat 148260aaatagtaca aagtttttaa agcacatggt gggaaagata tttatgttgt tcataatgga 148320ttttaaatga tcaagttaaa tgaaggtcaa attgctctat ttatattgga gaggatgaaa 148380tgttgtagga atgcctgcag ttacgttatc aactcaggtt gacttcactg ctttgaatag 148440tcagatttct ccctggtagt ttccaaatca gaacaatata ctcatttagg ggttgaagtt 148500tgaaaggaaa tcagatgatg cccatttaaa agatgattaa ggtttacata attctttgga 148560caaggggaca ttcgaataga tgtatgatgt tattaccgaa aacagaggtt aatagttgtc 148620tcactgttgc cttcttgact cagactttct tatttgggga ttttaattta cgattctgtc 148680ctgtacacag acaaatcttc acaagaatat aacaaacagc tttacttcta ttgttactat 148740tattaagaca gagtcttgtt ctgtcaccta ggctggagtg cagtgacaaa atctcgcctc 148800acaggaacct ccgactccct ggctcaagcg attctcctgc ctcaacctcc caagtagctg 148860ggattacagg catgggccac ctcacctggc taatttttgt cgagatgggg tttcaccatg 148920ttggccaggc tgttctcgaa cccctggcct caagtgatct gcccacttcc gcttcccaaa 148980gtgtcaggat tacagctgtg agccaccaca cagcctatat ttatttataa aagtttcatc 149040ttgagtttgg gtcaactgcc tctgctggta tcttgactcc atagtccagt tgttggcacc 149100aattcatctt ggtgctttag aagcctctaa aggcagttca tgatcattca gaacattgct 149160cagtgttgtt tctaatgctt gtattgttag tcttatcctg acctttacgt tgtgccagta 149220aaattccatt gattatattt tttcatttca tcactgtatt tgtgtactca tgccccactt 149280tttaaagaaa aaagtcatgt ttgaaagaca tgtacttgtg ttctaagaag tgatgcaact 149340atttttggaa aaaaaaaaaa tgaaacttta agcatatttc caaagttctt tttgtatcaa 149400cacttcttgc tgtgttttcc cagttacggt ggtatttgac aaacacctgg ctgatactaa 149460tctggtacac agctggacaa atcattttct taaaatggtt cccccaaaag gctaattata 149520aatataaatg cctgtaatta cttagagtat atacaaatca ttataggaaa tatagaggga 149580actgcattcg tatgatccac ttaacaacat tcacgatatc ctgtaatgaa ttcacacttc 149640aacttcactc agacttcaaa attatacaaa aaattaactc tttctcatgg tggagatagc 149700tgcccaaacc tgtaatggct gtcctctaca gtgaagttgg aaatagctac ttgagatgcc 149760atagacagcc aaagtccagt taagatcaat cctagactgg gtgttgaaaa atttctttag 149820gtctttcagg tttacatttg gaatctggac agttgtccaa aaaataaatg tataattgtg 149880catagtgatg ggattagtga ccctagaata tatttctgta acaattccac ccttgaatga 149940atgataaaat caaatagggc caaatatata tcaagcaaga agttctgctt tgagaactaa 150000tgaccaattt ctagtgatat tatcagaagt gttataaaac tttttttatt tcctgtattt 150060ctactgggga aagaggacaa gggcaggatg gataactcca agtacttcca atctctacta 150120acagaacaat attagatcat gcagagaagg aacatgtctc catctgattt tagtattgtt 150180tttcttagta ggacttttcc cctctctccc ttcctcctta tcttccttcc cttcttcctt 150240tcctccatct ttcttctctg tttctcgctt tctctctttg aatacttact aagtgaataa 150300aatccacttg attgtgcact acttgggaat aagaatcagg ttatttctag aatgatgaga 150360atctatctga tgcaaagaga aaagggaagt aaactttaaa aaagtttata atctataagt 150420gctttatatc tactatgtca tgtgatagcc accacctttc tatgagatta ttattataaa 150480ctttcatttc agagataaga agcttgaggc tcatctctaa tttatggctt aattaactta 150540cttcctcaag gcctatagat ttatgtggtg ttattagaat tcaaactcaa gtttgtctgg 150600ctacaaattc tgtatttcca ctgtaagttt caaaacattt tcaatgttta caactgactg 150660caaatagatt gatgttagaa attatgttag ccataagaaa ataattatag tttcttaaag 150720aaatgatgag actaaaatct tagcttcctt ccctattcgc ttctctcttt tttcatatag 150780gtagagatta aaattctctg aaactgtggg tattatttta gccagaaaac atgacaatat 150840agaatacctt actttaattt cattttcagt agtatatttg tagtttcttt tttttaacgg 150900tcatgtgttt tttcttcctt ttagtgttga atgtagaaaa cagaatttca aattcccaac 150960tatgtaacaa caaaaaagaa atatttgtat tacatgatag caaaatgcca tcaagagacc 151020ctattgaatt tactttctca cacagagaaa actgtaatag tgaaagggga aaaaaggata 151080ttaaacttga aatcagaggt actggttttg gaatatagac tcttccacct atcatatacg 151140tgaatttggg taaatgattt aaaagcttta agcttcagtt ccatcatcta tgacaaagat 151200cttaatgccc atttgcagga ttgctgtgag gattaatatg aggagactat gttaagactg 151260tctgaacatt tttaagagta atgtcgacat acagcactag tactgccagt ccccactcct 151320gaccccacag aagatcctgc caaactataa tggtagtctt tccagcagat ctggacagct 151380tcacaaaatg aagcctcata aaagtctttt ataaagactg gtccaataga ccttcctagg 151440ttagtggacc caaaaagtgg acataattaa gctagatgga tcctcttggc cagcctgggt 151500tcaggtgaga gatatccact gcccttttcc acctaaggcc taaaatacta agttggagct 151560ttagcccttt aacttaattc ctgttaaaat gcagctatct tagtgagtga tttctatata 151620ctttcctggg agcagcgtgt gacagaggag tggggacggg acagggaaag catgagttga 151680acacagaaga gacagaaaag catgcctatg actaggagat gcttggtagg cagaaccgag 151740gggatgaatg acagtgttcc ccatttctgt agtctcttca gattcagcca tgcccatgtt 151800gtttatcttt tgctatactt ttagtctatt taccattacc cctcacagaa atagctattt 151860tcattgaaca tcatctgtgg tttagcatcc ttctaggaac tttgcatatt aaattatagc 151920aagcttatac tgttattact ctttttcaga tgagaaaaag aagactcaga gagcaagtaa 151980gttgcaaaga tagtattaaa actagttgca tttgaatcca aacctcttca ctttctgcca 152040actgtactgt ttgtcatatt tccttaaatt gaaagttcgt gttttccttc ctatcaagaa 152100tgtgaaggaa cctatcccag tcacaaagat taggatatat agtagcagca gcaacaacaa 152160caacaaatcc ccaagtctta gcatttaaaa atgacaaaag gtctttgccc ccatataaca 152220tgtctgttgg ggatcagctg tggggacttg gcagcacatt gtcctcatca gagactcagg 152280ctattggagg cttcaagcat cactagactc cactgcacac agaagggaag aactgtatgg 152340cagctcttaa atactgccag accaagtatg tgatgccaac ctaaagaagt atggttgttc 152400ctcggtatcc actggggatt agttacatga ccaccttccc cctaccaaaa tcccaggatg 152460ctcaaatccc ttatataaat tagagtagta tttgcatata acctatgcac atcctcctga 152520acattttaaa taatctctgg attatttata atgcctaata caatgtaaat gttgtataaa 152580tagtgattat actgtatcac ctttttattt gtattatttt tattttcata ttgttatttt 152640ttattatttt cttttctcaa tattttgatc tgtggttagt taatctgcag tttgcgaact 152700ctcagataca gagggcaact gcacattgtg ggcaagtaca aacctatcgt gtgctgaaca 152760gagaagaaat ggaggtactc gtgagcagca ttaatgtgta caccagaatc tcaacagtaa 152820agacagaata acatgaagaa aataactaca ggagtgctgt gttttaaaaa agcaagctcc 152880tccagctgag tttgtgactc taatccctgc ttattttgag cactttccat gtgttgattg 152940actcggaaag gtgggagatt accagcaagc caggtcattt gggcaaacat gggcatattt 153000ttagcaaagc tgaacaaact ggttggtcac tcgaactacc caatttagac ttatagtaag 153060aatagcacat ggttcagaat tgccaagact gctgctttag tcaacagaaa ttttttcata 153120tgcatgctgg taatgcagtg catctctgga agaaatgcaa gagtatttta acatttggtc 153180cagaagaaat atgtaaaggg tgtttgtaga atttgatgcc ctcttcagtg acataaggtt 153240atgggtttgt gaatggaaaa tctgttattt gttcaggtta ttaaaacaca ttctaattca 153300acatcattag actggcaaaa aacttccact tttaggactc tataaaacct acattaacct 153360tgaattaaca aaactaggaa gtgtgatgta gtagaagggg ctttaaggaa ggcagggttc 153420ataaccccat cacgaagtta cttactacaa gaactttaat tctgaatctt tcaggccatt 153480tctcatctgt tatgtgagaa tattaataag taaatgtggc aagtactgtt gactctacct 153540tcaaaatata tcttctacta ttcatttctc tccatctcta aaactcctaa ttcaggcaaa 153600actaatttct cagctagacc accacacctg ctcctaatac ttctccactt gcccccacca 153660gcccactcat tactcagcag acataaggat ctttcctgcc ccaggttctt tgcacatgct 153720atcccacttt tttccctacc tcctgcatgc tacagctctg tttcttcctt tggttaacag 153780cttaagtact ttctcgaagc attttttctt gatattccta tctacagtag gtttctctat 153840tatttactat ctcagctcat ttgtttcctt catggtgcct gtcctattta gtaatttgtt 153900tctgtttgtc tgtctacctt tttgttttct atattagaat atgagtttcc caagggcaga 153960gacaggcttt gtcttgtctg tccttgaatc taccaagcac agtgcctggc acattggagg 154020tgctaaggaa atattagtgt aaagagtgaa ggaattgctg tgaggaatat gtgagttaat 154080taagtgcatc tttaggtacc tgggtagtgc tcagttaata ttgttattct tttccttttc 154140tttaccagtg ctgcccaatc aatctccttc ttctactccg tatatttccc tgctgaaggt 154200atactagcat gtttccccat catatgcaaa tatcctacaa atccaaagta aaaggcaaag 154260atattgaaat acagggaaca gaagaaattg ctcctgactt tgatacattg ctcttttaat 154320acatctgtgt ttttgtgaat tatgcactga aactcttaca tgacctttcc tacaccaaat 154380ttagaggttt gtaatcagca aacaagtgtt tgtttcttat gatttctttc tataggttat 154440acagtatctc taagttttct tgaacaatgg tgttaaaaat aattttaatg ctttattgtt 154500attttctttt attattcaaa aatgggtgga aaatgaagat gcaaaaatgc agaaagtagt 154560tataaaaagt taggtaagga acagtagagt taagacaaag gtaaaagtaa ttaagcatgc 154620aatcgatagt tagcatttag tttctattgg acacattaaa attaaactga ttaatagtgt 154680cttcatttac aaaggaagca aatttaaaga atagcatgaa atgggcctgg cacagtggct 154740tacgcctgta attccagcac tttggaggcc gaggcaggca gattacttga gatcaggggt 154800tttaggccag cctggtcaac atggtgaaac cccatctcta caaaataaaa acaacaacaa 154860aaaaaatgaa aattagccag gcgtggtggt aggctcctgt agtcccagct actcaggagg 154920ctgaggtgag agaatcgctt gaacctggaa ggtggaggtt gcagtgagcc aagatcgtcc 154980tgggcgacag agcaagactt catctaaaaa aaataaaata aaaagaagaa gaagaagaat 155040agcatgaaat gctggaattt attgggtatt atatctaaaa ggagaggcat atttatgaaa 155100tttaaatcag ttttagctct ctgtagaaca gaatccccac tctaaaatac attatctctt 155160gcatatttga ctttcttata tgtttttagt ctgattttgt ttgaaagtag tggattgcca 155220atacgcttgc tcctttttcc cgtgaaaggt gagcaaaccc accctgtcat ttacttatga 155280ggaacttgtg acccagtgag gtgaaggaat tagtcctact ggaatgcctc cattctttgt 155340aatcagttgc ctgaattatt ttagagtctg tttattttaa aggaatcttt catagttctg 155400gcaaaaattt atttataaat ggctttgagt ttgaggtaga aaatattaac tattagacag 155460ctgattgttc tagatgtgca agaagtaagg cactcaggat gtccttaact ttgttttcct 155520cttggttggt taatccctcc atatgcaata ggttgtttac ttcaatctgt ttctcattta 155580agaccactga aagttaatcc atggagctca cactctgtac cttctgaaat aactctaaca 155640aatcaagtta aaattttcct gacccttctt cgtgtcttcc ttcactcctt aactcctgcc 155700agtacgtgct gggaatttgc ctctggactt tgacacattt actcttttag atttgttatg 155760tcccacatta actgaacttt gccatattct taatgttgat ctggatttgc agaggtggtt 155820ttggagttct gcccatattc ccagtgtcta atccccagca agcctctctc tattttcctc 155880cactatccac tgccttacat taaattacca ttatgctagc tacatttcaa gttcttggta 155940gccacatgta actactggta atcatattgg acaggataga aagttgtatt gaacagtgct 156000accctagaat atctagacct tcacctacct gtgccagcgt cagtatctag aaaactagaa 156060tgtctcaagt gtccataaaa tcatttggca agttaagtct cagtaaaaat gtccttgatg 156120tttttttctt tgtagttaat gatgcatttc attgctttcc aaactctttc acaaggctta 156180caaaatccat catgaccttt accatttgtc taacttcatt atttctcttt cccttgaact 156240gtactctttt gaactatttt acattccttg actccaaggc taaacttgct actccttctg 156300tctggaatat tcttcctttc ctgttcagtg tctcaagtat tctctggtgc tatgggacac 156360tttaagagga ggagcttaat ccctttggac aacttagaga tgtggaatta atttaatgtc 156420gcttgatgat ttctatcatt caacatcaga aagcaattta gggcacaggc aatgtgaaac 156480tgcattttgt acacagaaaa ttatgaaata ctaaacttct taagggagga gccattaacc 156540tcagaaactt agattgttcc tattgtttta taagaaacaa atctttcgta tatttatccg 156600tagatgtgtg tttgtgtatg taactccctt ccgccgcata aacttttccc aacactcctt 156660taccccacgc ctgaagagac aatggaggag agaatggagg agctctgtga ataaaatgca 156720aactctattt gaatccagtc caatatttaa ctcaaagaaa aaattttaat actagccaga 156780acttttgaga agcgtgctct aattcgtcct ttctttttct ttatgacatc tgtctaaagg 156840gaacatttgt ggaagagact catccacaga gagagtaggg ttggcagaag caatgcagat 156900aggcttctgc agataggctg tggtccttcc tgtaaggcag catttcacaa ttcagatgaa 156960atgactcttg tcatttttct tttcagttat tgcaaaatct gcttcagtga aacatgctta 157020tctgtttctg tataattaaa aaaaatcttg tacaagtaac gaagtacatg aaatcaaaca 157080cacttgctaa actaatggca tatggagtta ttatgcacgt gagactcaag tctcagtgat 157140tagattatgc ctctcccctt ccattatgac tgcaaattgg tatgcagcca ttttcaaatt 157200agcgactgta ttagttagtt cacagcctgc tgtaaaggtc ttcttgagac tgggtaaatt 157260ataaaggaaa gaggtttaat tgactcacag ttccacagag ctggggaaac ctcaggaaac 157320ttacagttaa tggtggaagg ggaagcaaac acatccttat tcacatggca gcaggaagga 157380gaattgtaga gcaaagcagg gtggggaaaa gtccgttata aaaccatcag atctcatgag 157440acctcactca ctatcacaag accagcatgg aggtaaccac ccccatgatt caattacctc 157500ccactgggtc cctaccatgt ataattcaag cagagatttg ggtggggata cagccaaagc 157560atatcagtgg caacgtataa atctcaaagg ctcatgtcta gttttgcaga gcattggttt 157620tatatgatag cagttactgt ccctatgcag tgaagtgtga tacaggcttt ttctaaggca 157680ttacaggaaa cctctaccat ttcatcacac tcactatgct aaaagccatg atgaaatcca 157740tgactgtgct ctctagcact taattatgtt ttgattacta caaataattt atttttttac 157800attttgcttc ttagaattcc cagccacaaa cacccattca tcctatcaaa acagaacaaa 157860ccatagcaca tatatgtaaa catttttaga acatgtacac gtagataaac aagtgtatag 157920aacgtaacaa agtttctaaa ccatatcaca ttgtttattt tagtcaccag tactcaacaa 157980tgattcaaga aattcagacc ccaacagcat taatgtctca gcaatgtaac ctgaaactca 158040gcaacccaaa tctacagtag tgggaaagcc tgggtttgtg cattgtccga tgtgaaggtt 158100aattcgaatt atttttccag acatatggag ttctccttta ctttcagaaa aagaaagaaa 158160gaaaaagttg ttgcacacct actgtattag tccattttta cactgctgaa aaagacatac 158220ccaatcaaga ctgagaagaa aaagaggttt aattggactt acagttccac atggctgagg 158280agatgtcaga attatggtgg gagctgaaag gcacttctta catggtggta gcaagagaag 158340atgaggagga agcaaaagca gaaacccctg ataaacccat cttatcttgt gaggcttatt 158400cactatcatg agactagcat gggaaagacc ggcccccatg attcatttac ctcccactgg 158460gtccctccca caacatgtgg gaattctggg agatacaatt caagttgaaa tttgggtggg 158520gacacagcca aactgtatca cctactttag cttaagcatg gtgttagata tatccacaga 158580agttaccatg tttaatttta acaatgaccc tgagagtaag aatttttatc cctattttta 158640ctgatgagga aaactaaggc atagagcaga tcattgactt ccccaagaac acagtgctga 158700ggctgagata caaacctagt cctggaactc ttgagtttat tccttttcta atgtaccgtg 158760ctatcccagg tcactgaaaa cctacttatg gagttactgc acctggaatt atataagtct 158820gctcaaggac aagcaggggc aaatcaacca gtgaaaataa agtatagcag aaccgggaac 158880ataagacctg tgagcatgca tatctgattc ctggaatgca aatggtcagg accccagcca 158940aatgaactga aggttagttc tgggccacag tccaattgga aatagaagtg tcaaatagaa 159000aacctatagc agaagttccg gtaggcaaac tgtgccagtt gagtggacta ttggagatca 159060ttttggtgtg gtgggtccat gaacacccag gacctaccag atacaccagc tttacagcaa 159120cggactaact aattccagac tctgcagact ggcttactat atgcctgcct gttaaattgt 159180gtaagatgtt ttatgcagat tatctcaatg aaacctcacc acagcaggaa gaagcaagat 159240tgttttatta cttgccatag tttaacatgg caggttaagt agtggtaaag agcacgcact 159300gtagaaccaa gtttcctggg tctgtcttct ggttcttacc aattctgtta tcatctattt 159360ggggaaattt gcttaactct gtctgtgacc cacttcattt gtaaactggg agtgagatag 159420tgctcaagtt ttcagctttc caagagtata aaggcttcaa atggggcctg gctcaggaaa 159480ttgctaggca agtgtatgat aaataaagga gaaaactaag taacttgggt aaattcataa 159540aagtgatatg tggtggggct gggacttaaa tccagagata tctgactctg caagtgaggg 159600acctcaggga cttcacctct gaggggacta gggaaagcag atatcaacac aatcccaaga 159660gtcctcttat tttctctctc atcctatatt ggctaccgtt tcgatggaag tcatgaaatt 159720actaaaagta atttcagaat ctcctttgca taaggccctt tccaatgtta gctgtgcact 159780taaaatattt aactttgcaa taccaaatcc acctccatct tacttcaata ctattttgca 159840ttctctggtt tctgaggaaa tcacaaaaat gagccctagg caaaaatttt aggttagagt 159900atgaagaacc ctattgagtt tatgcacatc agtaatgcct tctccaaaat aagaaatatg 159960ctttcaattt acatcataaa atgagatttt ttaaaaagtt aaaggtttta ttgatgcact 160020tccttaaact agtatttcaa aagataaaaa cagcttttct ctgttaagaa ggaatggctg 160080ttttatgagt gttttcttct taacttgaca tgaagcaaat ttaagcaata tatagctaga 160140tctgttcagt ctcaccaata tatgagaaag taaattttca taaaaaaaac tttacctgtg 160200ccaaatcaag tcctattagg ttgtataaga tctgttatcc catgactccg agtccttgga 160260atgcatccca gttcagtcta gcagagttac tttctctgga cttcatatgc atagacatac 160320gcttggtaga cagagacaca gtgtgaattt gaaagcaggt attttcaagc aaaagccatg 160380aatgacagtt ctaaatgaga gcaaaatctc ctgtgttcct tttcacaaaa ctgcaaagct 160440gtaagaacaa cacgggtgtg ttttgttgca ttttcttgga tcagcttttg tctcactgtt 160500actagtctct ccaagtttga agaggccatg tttagtttgt ttttcaaccc agaaaatata 160560agaattatat catgaagtga tgcaatatgt ataacagtag ggaaattaag caaagatttt 160620gaagtcaatc aagcccctgt gggttcgaat agttcctcag ccactagcta actataggac 160680tttaatcaag ttatttaagc tccccaacag attacagtag aaacaatctg gagacttgga 160740gacctctcag gaggctactg cactggtacg gatgagaagt tctctttaga atggagatac 160800tacaatgaag agggaaaatc tacttatgat ttggcctttg ggaggcaaat attctgattg 160860gctctggtga caaggaccca aaaccctttt tacctaattt ctctgatgaa aatccactga 160920ttatagagca cagatttagt acttctgatg aagaggtaag gctataaaaa gacatatcca 160980caaatggcac aataggtagc atttttcatc ttcattcagt ctcagcagaa agttcttgag 161040catccactta tttcagatac agggttaggc attcaatttg gtgttaggta tacataataa 161100aacttcacca gctcaaagac atataaagat atgctatcca tgtttatctg gacttttttg 161160tttcaaacaa caccaaaatt cagatttaaa aaatgacatt tattgatgaa ataactgaaa 161220agtctaggac ctcagaaatg catggacctt ggtgctcaaa tgatgtgtga agaatctggg 161280tttttctgtc ttttgccctg acttcttcta tactggtttc attctcaaag gtactctttt 161340catggtggta acagggcaac caggagcttc aagctgttgt ctttactatt tgagaaattt 161400cagtggtaaa aaaattgctt tgacttctca caatttctca gaagttctag caaatattgc 161460tgatagagtt attgacctag tttaggtcat atatctctat gtcccttctt gagctaatta 161520ccttgtacta ggcttgaccc taatgagctt tatagactag aatagaggaa agtggctcca 161580aaaaagatga ttgaataatt attactagca tcaaggaaga atgacttgag tgattaaaaa 161640ttacatatgt caactatact aatttaaaaa ttacatatgt caactatact atctaattga 161700tttaagtaag tactcttttt tattgcaaat gacagaaatt cattccagat taaaccaaat 161760gaaatttgat ataaaaatgt catggaaccc aagagcaagg agtgccatag gcctcataag 161820gaaccgaaat gagaaaatga aaattaactg gtattactgc tctctctctc tctctctctc 161880tctctctctc cctctctctc ccttttcaca tctgtattta ggatatgtct ttgtcttcat 161940tcaaatatct ctccatctca atgcgcatga ttacccaaca attctcagtt tcagccactc 162000agaagagatc aaaactagtt tccctcactc tcaattcaaa atttgtggaa aaaaatatta 162060ttagttaaac ttgtatctag tgaccactct tgtccaacca agtatgacat agagactggg 162120ttaaaccaac tgcttggttt gagggtaatt cttggaaaaa gaaggctatt gtaatgttga 162180caaaatagca acagcagtcc actgcaacat cacaaccaac tttactgaca gaagatcttg 162240ttcctgctat aaaattcatc tttatcttct ggactttagt aaatctgcaa aggtatagaa 162300actatacctt taaaaatgtc cacaaaacaa tatgaaaaat ggcaagtcaa tctccaatga 162360tgaaatcgtg tatcaaaatc aggctacatg attgtcaccc tggccatata tgtaaagaaa 162420gtagacaaca aataaaaact caaactactt gattatttgc ttgctggtaa gttgcaagtt 162480gtggagaaga aattaatcca ttctaatgaa catttatcca tttcttatga atttccaaac 162540atattgtcag gagttaaggc agcagatttg tagacagagt cactttccct gaaagactga 162600cagactagtt gaggagacag acaagtaaat cactgattga aaaatagcaa agatatttga 162660taaatggggt atgctcagga tgctatgaca tttcagagaa gaaaatatcc tgcttcaagg 162720ggtatattag tttactaggg ctgccgtaac aaaataccca cagactgggt gacttaatcg 162780aaatgtatat tcccacagtc ttggaggtga aaagtctaaa atcaacatgt tggcaggttt 162840agttttgttt gtggcttttc tttttggctt gcaggtgaac accttctctt tgtgtcctgt 162900gcctgcacat cccttgtctc tctctgcatg ttcaactttc cttttcttat aaggatacca 162960gtcaaattgg attaaggccc accctgaaga cctaatttaa cctaattact tatttaaagg 163020ccctgtctgc aagtacagtc acattctatg acactgagag ttaggccttc aacacacaaa 163080tggtggggtg agggcgctgt gcacaatcca cccaaaacaa gagaaatttt cgagagtagt 163140gttgcttgac ccagtcttaa agaagagggt ataaccctgt ggatagaaca gcaggaacaa 163200aggtataaat gcaagagtga gcattgctta tctatggaat tttaaatagt ctggtaatgc 163260tggagcttag gatgcaaatg aggaattgaa aattggtaaa acatgaagtt ggaaaggtaa 163320ttaaggattg aattataaaa ggttttgtac tgagatgggc taaattatgg ttaaaaaatg 163380cctcaagata taatagcaca aacacaacac aagtttattt ctccttcaag gaatagttca 163440gggttggtgc tacaggtcca ccagcaactc tcttccacaa agtgattcct ggatcagact 163500ccattcatct caaggctctc atctgtgtga tcatgcctgg gtggctgtgc tcaagacttt 163560cactttaagg tctgaaccag taagtggcac acttcactcc tgctgtcctg ccattgctga 163620gaactattca catgacctct cctggttgct gggttatggg gattgtaaca taagggggtt 163680ggctggataa ccccttccca gctgtaattc tattattatg aaaagacagg cattttgtac 163740ataggagctg tcgtcatcac agtcttgtaa gtcatagtaa ggagtttggg ttcttataaa 163800tttgaggatt tttaaggagg acggtcgtga ttaaatttta attttaaaat gtaatgctag 163860tagcattata aagaaagaat ttgtgaggca atgtcagaag cacagatata atattgagga 163920agctatttca gtaattctgg caagtgattc tgaattaagg cagtgctagt aggagtggag 163980aagacaaaac agattccagt gatattttag aaatagactc atcatgactc ctgactgatt 164040tcttttgaga ggggaagaag aggaaaattc tagtaactct taattcctgg cttggatgaa 164100tgatgagatt ttggtaacat taaatgagga ggagagggct tgggaaaggc tcagttaaaa 164160acatactgag gtttgagata tctgtggaat atcaaagcag aggtgccagt aagtcataca 164220cataagacat attttcaggg gctgaaaaaa tacatttcat ggttattata atagtgacaa 164280aatgtaattg gaatcacatg gagatgaggt caactatagt aatcatagat ttatataatg 164340acataagaat ggtgatttcc aattttcatt ttatgcccaa acaaaaaagg tcttaggcaa 164400aacagaagag tttagatact accaaattat gaaagcagtg gtttagccat tttataagta 164460ttcaatggta aaaaacacaa aaatttcccc ttaaaattca gagtttcaaa ttctatagaa 164520agatattcat aaaacagaaa atcattacaa gttagaggag tttttagact tgacaactct 164580ttatatatga gtattgcatt agatatgcac tgtcattgaa gtagacattt aattaataac 164640cctatgctgt tgcaagtatc tatcaagtat attgaaaaaa caagaagcaa ccaattttat 164700tcttccaact atcttgctgg caaataactc gctcccttct ggaaatgctg aatcatttat 164760ctttacattt ttgttaacct gctcattttg taggataggc tcaagatagt tactactaaa 164820ttaaagatga tcatgtgacc atgagttaga aacatgaatc taagacccaa atgcaagatc 164880ctataacatg actgttaaga aaatgacaaa caactaagga atacccaggt gatatggttt 164940gtctgtgtcc ccactcaaat atcaacttga attgtatctc ccagaattcc cacgtgttgt 165000gggaggggcc cagggggagg taattgaatc atggggcaag tttttcccat gctattctca 165060tgatggtaaa aaagtctcac aagatctgat gggtttatca gtggcttctg cttttgcttc 165120ttcctcattt ttctcttcct gccaccatgt aagaagtgtc ttccacctcc tgccatgatt 165180ccaaggcctc cccagccatg tggaactgta agtcaaatta aacctctttt tcttcccagt 165240ctcgggtatg tctttatcag cagcataaaa acagactaat acagtaaagt ggtaccagta 165300gagtggggca tttatgaaaa gatacttgaa aatgtggaag caactttgga actaggtaat 165360aggcggagat tggaacagtt tggaggactc agaagaagtc agaaaaatgt gggaaagttt 165420ggaacttcct agagacttgt tggatggctt tgccccaaat gctgatagca atatggaaaa 165480taaggtcgag gctgaggtgg tctcaggtgg aggtgaggaa gttgttggga aatggagtaa 165540aggtgactct tgttatgttt tagcaaagaa actggtggca ttttgcccct gccctaggga 165600tttgtgaaac tttgaacttg agaaagttga cttagggtat ctggcagaag aaatttctaa 165660gcatcaaagc attcaagagg tgacttggat actgttaaaa gccattcagt tatataagag 165720aagcagagct taaaagtttg gaaaatttgc agcctgacta tgtgatagaa aagaaaaatc 165780catcttctgg atagaaattc aagccagcta gagaaatttg cataagtagc aaggagccga 165840atgttaatcc ccgagacaat ggagaaaatg tctccaggct atgtgagaga tcttcatggc 165900agcccctcct atcacaggcc cagaggccca ggaggaaact gatttcatca gctgggccca 165960cagtccctgt gttgtgtgca gcctagggac ttggtgtcct gtgtcccagc cactccagct 166020atggataaaa ggggccaatg tacagctcag tctgtggctt cagagggtgg aagccctaag 166080ccttggcagc ttccatgtgg tgttgagcct gtgggtgcac agaaggcaag aattgaggtt 166140tgggaacctc cacctagatt tcagaggatg tacagaaatc ccggatgccc aggcaaaagt 166200ttgctgcagg ggcggagccc tgatggagaa cctctactag ggaagtgcag aagggaaatg 166260tggggtcaga gcccccacac aggggcccta ctggggcact gcctagtgga gctgtaagaa 166320gagggccacc ttcctccaga ccccagaact gtagatccac tgacagcttg caccatggtc 166380ctggaaaagc tgcagacact caacactagc tcatgaaagc agctgggagg gaggctgtac 166440cctgtaaagc cacagaagtg gagctgccca agaccatggg aacttacctc ttgcgtcagc 166500ctgacctgga tgtgggacat ggagacaaag gagatcattt tggagctttt aaatgtgact 166560gccccactgg attttggact tgcacggttc ccgaaactcc tttgttttgg ccaatgtctc 166620ccatttggaa tggctgtatt tatctaatac ttgtacccct attgtatcta ggaagtaact 166680agttgccttt ggttttacag gctcacaggg ggaagggatt tgtcttgtct cagatgagac 166740tttcaattgt ggacttttgg gttaatgctg aaatgagtta agactttggg ggacagttgg 166800gaaggcatga ctggttttga aacgtgagga catgagattt ggaggggccg gcgtggaatt 166860ttccatatgg tttggccatg tccctaccca aatctagact tgtatctccc agaattccca 166920cctgttgtgg aaggagccta atgtgagata attgaatcat gggggctggt ctttcctgag 166980ctattctggt gacagtgaat aaatctcatg agatctgatg ggtttatcag gggtttccac 167040ttttgtttct tcctcatttt tctcttgccg ccaccatgta agaagtgcct ttcacctcct 167100gccatgattc tgaagcctcc ccagccatgt gccaccgtag tccaattaga ccaccttttc 167160ttcccagtct caggtatgtc tttatcggca gcctgaaaac agactaatag accagatgtt 167220tgaaaatatt tgaaatttct tagataatat taatgaattc aggaaatgaa aatggaaaat 167280gttttttttt aagacacagc attatcattg agtattttcc aaaatattta aattcttttt 167340cagtgagaac cattatagta cattttataa atcattgtaa ttttagtaaa ggtaacttac 167400aaattatatt tgagttcatt gtactgaaca actccatggt gatttgactt tccctgtcta 167460gtctggtact caggctcaag tatgatgttt tggtattgac ccttgggcac tgaatttgat 167520gtattagctt ctgatgctct tctaagttct gattttctat ttttatttct atagcatgaa 167580gcttttaaaa taagactttt gctaaaattg acattgacac ctcccacccc ccaccaaaac 167640acacactctg ttctttaaag aggagagtgt aaacagtatt tcaaaataga catcttttaa 167700tttggactgt aaagtctttc agttatacaa tttgagtttg actccaaaat gtcatatttc 167760atctcaaaat aaaggccaat actctaaata agaactaact cttacccatt tggtgggaat 167820caaactataa aaccaccttt ctattttttt ttaataaaag tgaaatatgc ttaaaagcaa 167880aatgagacaa ttgcttcaga tgatgcataa acataataaa caatgattaa cttttatttc 167940tttaaaaggg tattctattt tcatgtttta tatttaatta accattaaat aaatggttgt 168000aagatgataa agaaacagta tgtttttaaa agggatatag agagtcacag cactatgtaa 168060atagattttc tatgtgagag tgaccacttc aaatgactaa ctcacaagtt tccttgggac 168120taatgatacc taaaaggcag tttcaagtac atccaaattc caaattctca ggatcttctt 168180cacctaacta ttccttttga ttttctcagg gattcatctt ggtccacttt gcatagtgta 168240ctttatggat tatgtggctt aaatttatgt ccatatgctg atgattccca agtctctgtc 168300ctgttcagac ctcccacctg cacatccaac agctggctgg acatcatatt caatttaacc 168360ccacacctag tgcccgccaa ataaatgatg tccattaaat ggtttattgg gaatgttaaa 168420tgctttacaa aaacactgga ttataaacat gtttggcaac acaaaataaa aaccacatca 168480gcttacagca attttcatcc caaattttca actgccataa gtaatttcca cctgttatcc 168540ctccctcacc caaaattcac tatgcttata atcataacat tcccctctat cttactctga 168600gacaaatttc tagatctgat cctaatttcc tcgtgtcttt aaatggcgcc aaccttgttt 168660tgggccaaag gagccctatt caagtgtaca agttgggaaa aatatatttt gggcaggaac 168720ttcttcccct tttagatgct gtgtttttct gctcttgtcc cttcattctc ttcccctcct 168780actgaaatat tgctttcagc cagttgtcaa tttccttctt tactggattt taattgatac 168840agtatttact ttcctaggaa actgatgaaa aaaactcaaa agaatgactc tgcaataatg 168900aaattttaag cattatgcta aggataaagt agatatactt ggggagtgag gtctttcctg 168960ataccttcca aataatacag attccactac agagaagtat gaatttacag tgaagaacac 169020ctaaaatttg tctaagccac ctttgttctc ttgtgttaca tagtcttacc acaaacttcc 169080taaaatccta ttttatttat gttgctgtcc aattgaagaa catacaaagc ctcttccatt 169140ttgtaggcca ttgcaattta aaatgtctgt cagatcctca gtctgatctt ccctttacag 169200tcattttttt tcttcactat gtaaagtatc cagactttac actttttgga attgtactac 169260aggattaaaa tccccattct atacttgtta gtaaaattgt ttcattaaat gatgaattaa 169320taactgaaat aattaatgaa taaaacattg ctgtgcctgc tctctgcatg ccactactac 169380tctcacacca gcctctacac ttttgtgtgt gctattactc atacctggaa agtgcacttt 169440ctacctctgc agctatggct gtagtgtgca ctaaaatcac ctagagggct tggcaaagca 169500cacagtgctt ggtctcatcc tagcccagag cccaagggtt tgcactcaag caagttccca 169560acagatgtta atgatgctga tccaaggacc acactttgaa aacacagttt agagctcata 169620ttatctagag ttctgagatc cactttgtat tatttcacat gaatttttgt gatgtccttt 169680tctcatacta gatgtttttc atacttctag aaacttgtta cgtattggtg cattctccaa 169740aaacttggtg ctctcacagc gttagtccta accatgctga actttttaac tccctgtgaa 169800aagaccatgc atttttgtaa tttcctgcta ttcccttttc ccagcttact tcccattcgt 169860ttcatcttgg taaaatctaa gacctagttc tactgtcctc tcttttacca agactccttc 169920aaatttcttg gaaatctcca cccatcccaa tatctttgat cccatggatc tctgtacttg 169980tcaagaccac tgagaatatc tttgcatgca cattctgaat gcttaaaagg cagaaagcag 170040aacatatata tatatatata tatatttata tttgtatcct tagtaccaac acaatgtctc 170100aatagaagta ttttcaaagt atcccaatta tttgtaaaat atttgttaag taaaagcaaa 170160aataaacaat aaaagcaaac caaaatattg caaaaaggaa tgtgaactta catactgagt 170220tgtcatgcag aatattgaaa atcagagcaa tttaggtaga ctctcccaat aaggcacaac 170280ctcagcttct attcatggct aaaacatgcc agatagtata ttgatatatt tattgctatt 170340tatgtatata aataaaaact gagaagaaag agtaggcaga aggaaggaag aaatacctct 170400gcttagtcta aataatactg actagatgat aattagagga ggttaaagaa tatttagatt 170460cttgtatatt ataaatgtgt aggaaaaaag cattgctgat gtcattgaca atgagaactt 170520tctgaaaata gctcctatga cctcttatga attatttctg aatttaatgt gcttcttccc 170580aaacacagat ttgattcttt gcctcaaatc ctgtagatac tttcttgcac ttgactttag 170640gatcgaggca tcttggtggg ctcactatgg acttatttct ctttttctat ctccagaata 170700taaaccacat gtaacttcaa caatgctttg tacaagatgg atctgtttag agctggtgat 170760caacatttac cttgaagggc caaaagggaa agtttagaac tgctaggtta aacaaggtca 170820atttgaggaa gcaatcattt cgacggctga cattatgtct tgtaataaac ttggccaact 170880gttttttgtg gaaattttat tgtttctgtt gttatggctg cttctgtcag ggaaggtgat 170940ctggacagtg ttttagaaag catgcttttc ataattgaaa tgataaatca tcttatgtaa 171000ctatcacact cacttatatc ttcacattga ggtaggcaaa tctctggaaa gtaatgttgt 171060gtgtgtggcc tgtatttatg tatcaatgga gacatcctaa ttttccccac agttgatcca 171120tgagcatatt tatagtttag tatttttaaa tctatgttag tgatggacag gccactttca 171180ctgatatgga aactatgcag tgagaaactg agtttcccaa gcacagagtt aatgagaaac 171240tacagagtca gaggatatag aagattaaaa taagctagtg ttgccaaact aaatttcctt 171300agcagaagta gctgaattcc tgaaagggag ccagtcactt tgagacatgt gttttattta 171360cgctgaggca tcttcgtgat gatgcatatt actcctgtgt atactcagag acacatgact 171420gtgaagaaaa aaaaggaaca atatgcggta gcctaatgtt gttgggtgac ttgtcaaagt 171480cttggttgga gttgttggtt gagattattt tgctttgtgg gtagtttagt gtgtgctcta 171540atttgttccc ttttccaggt aattacgagt ttagttttct aatttttggt ttacaatatt 171600gtcaagataa ttaaataaga atatattgtg aagagattag cacaaggctg gcatatatac 171660attgccagta aatggagctg ttattataat tgtgagaaaa atgagatatt tgaagatttt 171720attctcaggg acaggagcca tcctattctt ccatgtgcat acttacttca gcacttagag 171780catacttcaa cttttcaatt aatttccatg aaagagtctg cctcacctat ttccaatgcc 171840tggcagtact gtcaccattt gggggacagg gtagtaaatt aaaaagttaa tgtttctcca 171900atttctgagt taaaaaaaac ttccattacc ccttccaaag tcattatttc taaggatagc 171960taggcattgg aagaaaaagt atagagtgac tctagctatt aaaactaggt gattataacc 172020gctttcactc ccagtgtttg ccagaggaca aattgttgcc aacagatgaa caaagaaaag 172080ggtcttctgc ttctgaagta gtcactttct cccttcatgt tcccagcaac tgtttatttg 172140gtacctccta tgcgctgagc ctaggcaaat aaaaatgaat ctgagatcat ctctgtgctg 172200cataaaaact ttgcattgtt taatgatgtt tatttgggga aaatatttgg gaaatatatt 172260aaatgatatc ggcaaatatt aaggagaact tactttttac aaagcacagt gactttgagg 172320gaaaagataa taaaagaagg tatgatcttt tttatgatct ttttatgata gggtggagga 172380aatggaatta aatctaggaa actcagtgat agcagtcagt cacaagcaag aaataaagtg 172440ttatgagaac tcagagagga cagagtttat tcctgtctta gatgaatctg caagacttca 172500gagaggcaag ggaaactgct ataagtctta aagaatagta acatcattca ctcttcaatc 172560aagcaattat taagccatta ctaaatacca ggtattactt tacacaaata agtaagatac 172620agagaagaca cagctttgtc ctttaggagc tcatggtcta gttatagaag agacaggtat 172680ataattaaat aattataaca caatagatat aatattaaaa tataacatat gatactgata 172740taatttagta tagttactga gagaacccct gactctgcct tcagtctaca gttgtgtgta 172800tgtgtgtgtt cgtgtgtatg tttttgtatc tctatgtgta tatgtgtaca gaagggaaat 172860ttagaaagtc tttccagagg agacatttga actaagtctt gaggaagggt agaattcagc 172920aagcttccct gagaattggg ggcatagttg aaagagggag gtgatatagt ttggctctgt 172980gtccccaccc aaatctcatc ttgacttgta ctcccataat tcccacgtgt tgtgggaggg 173040accaggtggg agataatata aatcatgggt gcaatttccc ccatactgct ctcctggcag 173100tgaataagtc tcataagatc tgatggtttt atcaggggtt tttgctttct catcttcctc 173160attttctctt gccgccacca tgtaagaagc acatttcacc tcctgtcatg attctgaggc 173220ctcctcagcc atgtagcact gtaaatccaa ttaagcctct ttttcttccc agtctcagat 173280atgtctttat cagcagcatg aaaatggact aatacagtaa attggtacca gtagagtgtg 173340gtattgctga aaagataccc aaaaatgtgg aagcgacttt ggaactgggt aacaggcaaa 173400ggttggaaca gtttgggggg ctcagaagac aggaaaatgt gggaaagttt ggaaccacct 173460agagacttga tgaatggctt tgacaaaaat gctgatagtg atatgaatga taaggtcctg 173520gctgatgtgg tctcagatgg agatgaggaa cttgttggga accgcagtaa aggtgatcct 173580tgttatgttt tagcaaagaa actggtggga tttttcccct gccctagaga tttgtggaac 173640tttgaacttg agagagatga ttaaaggtat ctggtggaag aaatttctaa gcagcaaagc 173700attcaagaca tgacttgggt gctgtttaaa gcattctgtt ttaaaaggga aacagagcat 173760aaaagttcag aaaagttgta gcctgacaat gcagtagaaa agaaaaaccc agtttttgag 173820gagaaactca agctggctgc agaaatttgt ataagtagca aggagctgaa tgttaatccc 173880caagacaatg gggaaaatgt acccagggca tgtcataggt ctttgtggca gcccctccca 173940tcacagacca ggaagcctag gaggaaaaaa cagttttgtg gaccagggtc agggttccca 174000tgctgtgggc agcccaggaa cttggtgctc tgcatccaag ctgttccagc cattgctaaa 174060aggggccaac atacagcttg gcccatggtt tcagttggtg caagccccaa accttggcag 174120cttccacgtg gtgctgagcc tgcaggtgca cagaagtcag gaattgaggt ttgagaacct 174180ccacctagat ttcagaggat acatagaaat gcctggatgc ccaggagaaa gtttgctgca 174240ggggcaggac cctcatggag agcctttgtt agggcagtgc agaagtgaaa tgtggagtct 174300gagcccccac acagagtccc tactggggca ctgccgagtg gagttgtgag aagagggcag 174360acgtcctcca gaccccagaa tggtaaagcc actgacagct tggactgtgc acctggaaaa 174420gctgcagaca ttcaatgcca gcctgtgaca gcagccagaa ggggggctgt accctgcaaa 174480gccacagggg cagagctgcc caagaccatg ggaacccacc tcttgcatca gggtgacctg 174540gatgcgagac atagagtcaa aggagatcat tttggagctt taaaatttga ctgccctgct 174600gatttcagac ttgcatgggc cctgaaaccc ctttgttttt gctaatttct cccatttgga 174660atggctgtat ttacccaata cctatatccc cattgtatct aggaagtaac tagcttgcct 174720ttgattttac aggcttgtag gctgaaggtg cttgccttgt cttagatgag actttggact 174780gtggactttt gggttaatgc tgaaatgggt taagacttag gggaactgtt gggaaggcat 174840gattggtttt gaaatgtgag aacatgagat ttggaggggc caggggagga atgatatggt 174900ttggctctgt ccccacccaa atctcatctt taattgtact cccataattc ccgtgttctg 174960tgggagggac caggtgagaa aatttgaacc atggggcggt ttccctcatg ctgttctcat 175020ggtagtgaat aagtatcatg agatctgatg ggtttatcag tggtttctgc ttttgtacct 175080tcctcatttt ctcttgccac tgccatgtaa gaagtgcctt ttgcctccca ttgtgattct 175140gaggcccccc tagccatgtg aaactgtaag tccaattaaa cctctttttc ttcccagact 175200caggtatgtc tttatcagca gcacataagt ggactaatac aagaggaaaa caaattttct 175260caggaaactg gaaagaatgt gagtgtatga gtacagtgta aggagatgga gagtgagacc 175320tgggagtggg aattataatg tcaattatga tttagatatg agggagaaga aactaaaact 175380caggtaagga caagataata agaggctttg tacaaaacag cagggaattt ttgaggtggt 175440tgtggttagc cacagggtga cagccctgcc actggggagt ctgggtcagt aggacggagt 175500ctgctttacc catgccccaa cttcagtcac ctcttgttcc cagtcccaca cagctgtgtt 175560gcaaatatat gacctcaatt ttaggagcca tgaagccaaa atctattgtt attgtttgaa 175620atactttggg aacatgaaat aacactttac ttagtttgga actaagtaag cttcaccttt 175680tgatatgaat gtaatttgcc tgttagcttc atcttttgat ataaatgtaa tttaggtctg 175740aaaatcaaga gacataaaac agagcaatat aagaggaagc atttctaagc tgataagaga 175800tcttatattt tttgagtaat atagttcaga atggtttctt ctaccttgag ttactcagaa 175860tagttaattt ccttttttct tgaaacagaa tatttgtcca tatttcattt ccgtttaact 175920catttatctc tctcactgca gaggtttccc tacaaatact ttttctccac tggaggcttt 175980gggaataaat aaaacattgg agataaattt gcaagttaaa actattgttt atttctccat 176040cgttcgtcag taacttaaag ggtaacagag ataagagcta ttccttggga acttctggga 176100atttggtagg ggattatctt ttcacctgtt ttccagttca tgaatataaa cactgctcag 176160agagacctgt tttagggtaa aagcaatgtt attttgtaca cagagtgctg tgtattacaa 176220agtatttgat taaccaggtt gaccagtaac aaagttaatt atttagtcaa tattttacta 176280agtgtttcag tcattaccca tttaattttt aagtacttta tttttctatt ctctccatct 176340acacatctga tgttttacat gaatgtattt aaattatgaa atcaactgca aaagctagcc 176400acaagatttt aagattagcc cagtaaccaa gatgttactg ctgaatgata gacaagaact 176460ccacagtttt tacagtttga aggtaaatgg ggaaaaccct tttgaaggtt aatgtcatag 176520gaaaagtgcg gaatcaaaac acagctgtat ttattcgtct ttagagctat tttatttcat 176580aactttggat tagtcattta aaaatgccac agaagtcata tgttttctat gacagaagat 176640aataatacac acaaggtcaa tttacaaaac tctagtttgc agctgtcctg agaaacggag 176700ttctgaaaaa tctctgatct ggaggactta gttcaaaata acaagcagaa attttgttta 176760agtcaagtgt tagatttggg ttccctagtt cctgggttgt gaatatatgt gtttatatat 176820atgtatgtgt gtatatatat agtgtgtgtg tgtgtgtgtg tgtgtgtgta tctgtaaatt 176880cctagggtgg aaaaacaggc taatgaagca atgtgaattt ctgaaatttt ctattgtgta 176940cttgttttta aattataatt atcagtaatg aatgctaatt gtagcaagtc aaacaataca 177000gagcatttga agaaaaatat ttaagtttct ccggtttcca attccttttc ccaagaatag 177060aaagtctctt gcgcatatcc ttcgtttttt gttttgtttt gttttcagac ggggtttcat 177120tctgtcaccc aggctaaagt gtagtggcac tgtcatggct caatgcagcc tcaatctctc 177180aggctcaagt gatcctccaa ccttagtccc ctgagtagct gtgactataa gcacatgcca 177240ccatgcctgg ctaatttttt attttttgta tagatggggg tctcactata ttgcccatgc 177300tggtctcaaa ctcctgggct gaagtgatct ttcccccttg gctcccaaag agttgggatt 177360gtaagcatga gccactttgc ccagcttcct cttcatttct tccccctaac ccactttttt 177420tttttgtaat caaaggtatt ttaacctcat taacatgatt tataatttgg aatttctata 177480caaggcagta gttttcagcc ctactgatat ctcttacaca catttatgta gacatatata 177540taaatatatg tctataaata tatatgtcta taaaaatata tatgtctata aatatatata 177600tataaatata tatatatgat gaatggattt cttcattgtt aacagaaatg tttcataatt 177660tatttttaaa gccataatct ttaaacagta ctttatttac atagtaccta gtagataaac 177720gttgctcagt atttgtggaa tgaaaaatga tatccatcta accattagaa ttagctctag 177780cacactccaa ggcacaactt aaaaatcatt agatcagtgt atagggtaaa tacaagaagc 177840taaggcactt ggtttagagg tttgggtttt gcatagcccc tctgtgtgtc agttacaatt 177900tctattttac ttgatgtatc tggagacaga taacatcatt ggccacttgg ctgtttcata 177960ttttcctgtt ctcttctatc cccctgagag tttctccaga gtctccttta tggtaaatga 178020cctctgggaa tccagcatag ctctcttctc cttttttact tgatatgctc ttttttgaat 178080gatgtcatct acttctgtgg ccttagttac tgatgtgttg atgatattta gatctatatt 178140tccagagcct gccattctcc tgagatccag gaaatatttc taaatgtggc tgaacaagct 178200catttttatg ttatgtagtt gcttcaaact ctccaatata taatgctatc ctctctatca 178260cggtattcca tagctatgct ttctttttta ctgtcttctc tatcttggat aatggcattg 178320gttgttcaag ctagaaaatt aaaagtcatc ttaaacaatt gtcacttctc ttacatatca 178380tcaactcctg taggttctat ctgtaggttc caaccaaaaa gagtccagga ccagatggat 178440tcacagccga attctaccag aggtacaagg aagaactggt accattcctt ctaaaactat 178500tccaatcaat agaaaaagag ggaatcctcc ttaactcatt ttatgaggcc agcatcatcc 178560tgataccaaa gctgggcaga gacaaaacca aaaaagagaa ttttagacca atatccttga 178620tgaacattga tgcaaaaatc cccaataaaa tactggcaaa ctgaatccag aagcacatca 178680aaaagcttat ccaccacgat caagtgggct tcatccctga gatgcaagga tggttcaata 178740tacacaaatc aataaatgta atccagcata taaacagaac caaagacaaa aaccacatgg 178800ttatctcaat agatgcagaa aaggcctttg acaaaattca acaacgcttc atgttaaaaa 178860ctctcaataa attaggtatt aatgggatgc atctcaaaat aataagagct atctatgaca 178920aacccacagc caatatcata ctgaatgggc aaaagctgga agcattccct ttgaaaactg 178980gcacaagaca gggatgccct ctctcaccac tcctattcaa catagtgttg gaagttctgg 179040ccaggacaat tagtcaggag aaggaaataa agcgtattca attaggaaaa gaggaagtca 179100aattgtccct gtttgcagac gacatcattg tatatctaga aaaccccatt gcctcagccc 179160aaaatctcct taagctgata agcaacttca gcaaagtctc aggatacaaa atcaatgtac 179220aaaaatcaca agcattctta tacaccaata acagacaaac agagagccaa atcatgagtg 179280aactcccatt cacaattgct tcaaagagaa taaaatactt aggaatccaa cttacaaggg 179340acgtgaagga cctcttcaag gagaactata aaccactgct caatgaaata aaagaggata 179400caaacaaatg aaagaacatt ccatgctcat gggtagcaag aatcaatatc gtgaaaatgg 179460ccatactgcc caaggtaatt tatagattca atggcatccc catcaagcta ccaatgactt 179520gcttcacaga attggaaaaa actactctta aatagttctt aaatactcca cctcatataa 179580tttgtcccta caaccactat gttgggtcag aagctcaaca tctctcacat ggattgtctc 179640tcctctacaa tgttgataac aatattttct ttttaaaaca gaaatatgag cattttattt 179700cactactaga aaagtatcag tgacatcctt tgcctaattt ggtagtctct gtgtagcccc 179760aggggataca ttgtggttca aaaatgcaat attgagattt ctacttacat tcactctttt 179820ttagtgcaat atttaatatt cactaaagaa tgtaacatat attttataaa gttcaataat 179880gaaatgaatg cctatgaagc cactaccgac ttacatccta gaacattgcc agaactgttt 179940catctacctt cacttttacc tctctcttcc tcttgcttcc actaacaaaa ccccacttaa 180000gataaatttc atttgttata ctttccttat ttttcatgta tttacaacat atgtatttat 180060ttataaatat tatgttgttt agtttgttct tgaacatttg aagatttact cttatttttg 180120catgaagctg tggttcatta atttttttgc agaattaaat tttattccat taagtgacta 180180catcaaaaat gatttgtcta ctctcttttt gatggacatt agcttcttta taagtttagc 180240tattatgtac aatgctacaa tggataatat tgtatatcat cttgttacac ttgtatgatt 180300ttcaagaata tatccagcag tgcatttatt gtattacagg atagacaaat gttcaaagta 180360taagatatta gtagtgacac atatacatag cttattttaa aatgtattaa agttatatta 180420agagtgagtg cgtagctgag catggtggct catgccttta attccagcac cttggtaggc 180480tgaggcaggt ggatcaactg aggtcaggag ttcaagacca gactggccag aatggcgaaa 180540ctccatctct actaaaaata caaaaattag ccaagcatgg tggcagtcac atgtaatccc 180600agttactcgg gaggctgagg aaggagaatc gcttgaatct aagaggtgga ggttgcagtg 180660agtggaaatc atgccactgc actccagact gggtgacaga aacaatactt tgcatccttc 180720aatccaatca tgttaacact caatattaac catcacaata aggttaggtt aaaacagaca 180780gataactgaa ctaggaatta taccagattt gtctcagttc atctttggct agccatattg 180840ttacaggaaa gaggacagga ttcagacccc aagagagggt tcttggatct cacgcaagaa 180900ataattcagg gtgagttcat ggattaaagt gaaagcaagt ttattaggaa agtaaaggaa 180960aaaaagaatg gctactccat agacagagca gccatttaat ttagagcagt ttaatttaag 181020agaatgttta gtaagtttct cctgaattcc tacaaaaaga gtacaatagc aatatattcc 181080acaagaataa agcaaaagaa gtagaatttt ctcaagtaaa caaaattaga aggctttcca 181140tgaatggggc aactgttgga actaagctaa tatgggactg ttagctgatt ctaatgtgcc 181200cacaattaga atactcatac agatttttac attacccatc cctcttggtt gttctgacca 181260gcagtcagag atcactggtt ggctcacaag aataagcagg gttagcctaa attacagaaa 181320cacatttaaa aacaactgat gagactaaaa tttaataaca agtataccat agttcttgga 181380acataatttc tctttccagt ctcccatttt tactaaagac aaatcatggt aagattgatt 181440tgctttatta tatttggcct gattatttgt ataaagtata gcaagaataa atatttgcca 181500taagcttttt aaaaaatggc ttcaatggaa ctctgttcca tagaaaaaat cttagataaa 181560taagactttt tttttaagcc aagccctacc atgtgtatcc acactcagat acctacaagt 181620tgggtaaatt cctctctttt tgaggtccca agataacttg gggctcctgg gcctgtgatg 181680ttctttactt accacaggtc aggaagctca tacagggatg gtgtagtcaa ggtgtgaggc 181740cagttcccca agggactttt attggctcta caagtcaaat ttgatttctt aaagaaaagc 181800atgccattca ggtaaaagcc ttggtaaaat aaccagtttc tccaattgtg tcccgttgca 181860aaagaaaaca gattcttatt gcagtcatgc aaataactat gttgccataa attaagaata 181920ttcacagata gtttccaaat tctggataaa actggtagag agaaacaaat atgctccaaa 181980ttttgttcat aggagtatac tttactctat tgctacaagc tacaaatagc ttaaaggaaa 182040agttttccta actctgaaaa acaaaacaaa ggatcagtaa tgttttaaga aaaatttaaa 182100agattacttt agacttctat tagtttagtc catgcagtta acttctgttt aatattcatg 182160aacatttcag ctttccaaga gtgttctcaa agatttttct ctattctaac gtaataatct 182220ccaaagttat cagaaacctc catttaagaa cacctgttag aattgtacag ttgactataa 182280aatcatcttc caaagaggat taaaacagga caaaaattgt ctgtggatga taaaaagtct 182340taggacagcc actattaagg ccacaattga tatggaaatt tggtcacttc tgtggcatat 182400agcaatttca cataacaatt ataactatta ataacataca ttaagccata tcagaattag 182460aggagtttcc cataattttg gaacatatac caataacaca tttatacaaa tagagccaaa 182520aaaaaaaacc aagcaccatt ttgtatttgg caatgcttct tgtatgattt ttataccaag 182580taagccaaat gtcactgttg tgttagtgca ttattgatgt tacacccaat agacaaatat 182640attcaataaa accttataga caaatatatt caatcttaat cagtttgacc ataggtaaga 182700ttttaataaa ccttttataa gcctttacaa tttttcttac aggtcagatc ataagcaggt 182760ttttgctcta agaaaatctt gttatgcttt tattctaatg ctcaatttac agaaaaaatg 182820gaataatacc cctttaactt tagccagtgt gttcacacac agaatttctt ttacaattaa 182880tttttcacaa accttccaca acttgcataa cacttcagct ttattctaac ttaaaacaat 182940cttttaaccc tttaatctag gcagaaaaat tcatattccc aagacttctt ataatttttt 183000tacaaaaaac acattttact ttctttacat cccttgtgta tagaactggt ttttttttag 183060tagtctcaga tacatgttat ggtgttaact cttagcaact tttttttttg gtgaaagcct 183120tggtaagtaa gggatttttt aattatgtac tagtgtggag cctaggaccc agacagaagt 183180gcagataagg gctggctttt tccagcacag ctagtgggca tggctaactc cacatgtccc 183240aggccttacc tagctgtaaa gcaaggaaat tgtatagtaa gagccatagt ggcattttat 183300gaagcattta ggaggcccat cacctttaaa ttgtacaatg tttcttgcat aaattcgttt 183360tcataaattt tttcatgact cacacagacc atctacaaca tgcttggact ttctgacttg 183420tcataaacct tcctcttgtt aaacaaccag tcattttgct ttaggacaag aatttaccat 183480agaagatcgt ttcttatata aaatctcttt ctttataacc ttctttgcat agctagaagc 183540tatgcttatt tcacatgtcc ccaggcatta tctagaatct gatatctcca aggaagatta 183600aaatgaacag tttttaaaag ccaaagaagc agtttatgac cttaaagcat ttagcaaact 183660taatatctga cctgtataat ttagacctaa tgtttacatt tttgaaaaca ttttaatttt 183720accaacaaaa ttgtccttat tttaaaactg tctttatttc ccaaagatta cttaagtcac 183780atgaactaaa aggcattaca cattttactt ttctgacaaa atatttgatt tagaacttat 183840ttttaagcca atcaatcaaa gctctttcat atataaatat cacacaaata atacatataa 183900atacatagac agaagataaa ggaccaattt tccaagccag gaattaaacc ttgaatccgg 183960gctgccattg tgatggcaga gaccaagaga aagtactgcc acctggttac aaggtcaagc 184020tcccaaggac atacaagaga caagagggaa acttcatcca gttattttta tttttattta 184080tttatttttt tttagggacc tgcagcaaag tttataactg accagtttgc tgggccatct 184140tgaaaagcag gtttaggggt gtcctaagcc catgttctgt tctgaggtat gccttattat 184200gacagaacaa tacagaaaga cccacaaagc acagcagatt tgctacagcc tatgactagc 184260ctcacaaatc cttttttcca ttaattaaaa ctttacagag aagataaaca gtgattttta 184320ccattcattc gacaggtttg cagagagaga gaaagaaaag cattgcctga ggcaagatgg 184380ggaaagcgag gcactcaggg aggccaggaa gacccaccct ttgcagtgac actgaaaagt 184440tcgggcggcc acttgtcggt tttctccttt tacattaagt gatatacact tgtgtttctc 184500tgagtacata tctacctctg ctactagatt gtaagctcct tgagggggaa gactttccta 184560atttttttaa ttgctggtac tcagaatagt atgtacaaca taatagattc ttagcaaagt 184620tgctcttttt tactcatttt tcgaaaatca tattgagcac ctattatgct tatttataat 184680gtaaatgatg atatgtgtga acaagtataa aggacatgaa ggattttaac agtgatagca 184740ctgcatgtat ctaaacatta ccagcttctt gagcagtctt gtctgtaatt agtttatttg 184800taagagtaag agttagtctt gtatagaata tgcatagaga caacaacata caacaacata 184860caaaaattgt caggattctt gatttcctct ttagtgcttc ttgcccttct tcatttaacc 184920acatttcaca gtcactaata aaatatgaac aactgatgtc aaattcagga tcctgtttca 184980gaagggaaaa cagaaacatt taccttgttc ttaggacatg ctatgctctt tcacaagtgt 185040ctgcccatct tgactagtgt tgttgaaatg gctttcttaa tcaaataatc tcaatctcca 185100gctttgtagc tatatctaaa gttcccatat tacctatact ttgggctata gacactgata 185160ccatgtgtca acatgcaaac ataatctaga gaggaaacaa agtaaaaacc ttaattaatc 185220attaataact ttattcaaat tggtttctga gatttctttt agtaagcaga accctaaaaa 185280agtgaaacct tgtcaaaatt atattggaac cctgctcaat cgcagtagga ccttgaagtg 185340aagtcctgaa cagctgagtt acacaagggc tagaaatctc tccagttctt catttctcca 185400gcctattgaa agggtgccat tttaacaaga aagtaaccac aagtctggaa gggcatgaaa 185460gatgagctta gtctggagta gagaatgaat ctccaacctc aagtttcttt agagacactt 185520aatttgtttc ttgaacattc atttcacctt gtcactcata ttcttcattt caccttgtca 185580cccatattct tctgaaaagt ttgtgacagt ttctacatat atcagacaaa ttgagacaac 185640tgtcagcatt aattgggcct caaactcagc cccatgaaat atcagaatca acagagtatt 185700tgcaaaagac aagaaaatga ctgtgagcca ggcttgcccc agtaaaaccc agagctcatt 185760tataattggc aagatatctt accttaggct agcggaactc aatacttttg gtaagatgtt 185820taatgatttc tttacaactg taataactgg gtgctcacct caattctctg taattatttt 185880tcttatgaaa gcctagggca atacaaatgg aactctccct gaaactattc tggcttctaa 185940tgattttcct ctctactaca cagagtatag aatagtaatt ccattaaata cagattcagt 186000aagcaatggc catatcttat attaatcctc ttttatcccc cagttgtagt aaaactcaaa 186060gtgaaaaaga gatttttctt cgggagaagc agagattaca aatgcccact taacagactg 186120aattttagtc actgtgggtt atcatcacaa tgtttgctac ctgaattgaa ataacattca 186180atacatttaa atacatacaa gtagatgtat tagattggtg attaaaatct aaatggagtt 186240ttctttaggt aaatatgctt tgtaagatat gctttctatt ctgtattttc tgaccagctg 186300tgttcccaga tttctcagct cctctcagct cactggaaag gaaagatatt tcatgatggg 186360ttctgagaac tagaaaatat cagggaacag actctttgtg gcataatcca aaacacctcc 186420atctaaatat acagagagaa atttcctttt aagaaaggaa agaactggca aaattattac 186480tttttcatca tttcaaaact cagggtctat ttggattggg ttagatccca acagactaaa 186540ttgctggaca aagttgggtg gtggtttaca tttttagaga aataaattgc aatgggcctg 186600gcaggggcag agggataaaa gaaatataga gcccatgctt tacaaatggc ctacttgaac 186660agaatgtagt gtcatttcaa ataagcccag gcaggagtcc caggctctgt cattcatggc 186720tctacttctt actacaaact ggaacctcta tttgtatctc tgaatacgtg tccaagaaac 186780tataactatt tcattgaaat ggatattgca ggcttaatgt aatagcaaat tgtcctaatt 186840ctctgcttca tttttcttag gtatttatat accatgtatc tagtatatag ggagagctac 186900ctatatatta gcaatcatca tttgcttcaa gaacttcaat agaagtacat cacaaattgg 186960aaagctattt gttcatgttc aaaattcaac tgtagaatca acacattggt gctgtcagga 187020gctagaggat cacctattct aatgttttct gggttttggt gaggagagta aggcccagat 187080tgtgaagagg cttgctcaag atcatacagc aagttagaag gataaccagg tctggatgtg 187140agaattctga accctatttt ctgtgactca agtccggaat gatcattgcc ttgattaaga 187200tgctctagag aaaaggtaga ttcttgtttg ggaaaaaagt gaacagcttc caaattagat 187260tgactttgta ggtagtctcc actttattga ttgtttcctt gactgtgaag aagctttttg 187320gtttgatgta atctcatttg tgtatttttt attttgttgc tcgcctgtgc ttttttagat 187380cttattcaaa aaaattattg ccctgcccaa tttcatgaag catttctcct gttttcttct 187440agtagtttca tatcttcaga ttttacattt aagtatttaa tctatctaga attgattttt 187500gtatatggtg gagagataga ggtctagttt cattgttcca catgtggata ttcagttttt 187560ccggaacaat ttattgaaca gactatcctt tccccagtat gtcttgtgtt gttggtacct 187620ttgttgaaaa tcagttggct gtaaatgcat ggatttattt tgggattctc tattctgttc 187680cattggtcta tgtgtggttt tatgccagta ccaagctgtt tagttactat gggagaagca 187740tttgcaaact atacacctga cttgcaaact atacacctga caaggcatta atatccagat 187800tatataagga actcaaataa ctcaatagca aaagaactca aataattcca tttttaaaat 187860tggcaaaaga acgaagtaga tagttattgg aagaaaatcc aaatggctga cagatgtatg 187920taaaaatatt caactttact aattatcagg aaattgcaaa ttaaactaca atgagatgtc 187980accacactcc agttagaatg gctattatca aaaaggcaaa aaataacaaa tgctggcaga 188040gaaaaaagaa ctcttatacg tggttggtgg aaatgtaaat tagtacagcc attatggaaa 188100acaatttggg ggttcctcaa aaaaaataaa aatagatcta tcatataatc cagcaatctc 188160tttactgggt atatatccaa agaaagtgaa atcagtatgt cagagagaca tgtacactca 188220tgtttattgc agtgctattc acaatagcca agatatggaa tcagcataaa tgccattcta 188280tggaattgat tgtatacaca cacacacaca cacacacaca cacacacaca cacaaaataa 188340aatactattt agccataaaa cagaatgaaa tcctgtcatt tgaagcaaca cagatgaacc 188400tgaaggacat tatattaagt gaaattaaac aggcacaaaa agacaaacag cacatgatct 188460cttgtatgtg gaatctaaaa aagctgactc tgaaagcaga atctgaaata ctagtaacca 188520gagactgagg agagaggggg aaagcggggg ttgcaggggg accaggaaag attggtcaat 188580gtgtacaaag ttacaattag acaggaagaa taagttttgg tgttctatta cacagtagag 188640tgactctagc aaataacaat gtagtgtata tttcaagata actagaagag agaagatttt 188700aaatgttgtc acaaagaaat gataaatgtt tatagtgatg ggtaaggtaa ttaccacaat 188760ttgaccgtta tatcatgtat acatgtgttg aaacatcata ttgtatccca taaatatata 188820caattacgtg tcaattgtga atttaaaagt catattaata tccaaacaaa ttaagttagc 188880tttttaaaaa aaattttcaa cttttacttt agattatata tgcagatttt ttatatgggt 188940atattgcaac caggtagtaa gcatagtgcc caataggtag ttttccaacc cacattctcc 189000tcctccccca cgcccagttg tctgcagtat ctattgttcc cacatttatg tccgtgtgta 189060ctcaatgccc cacttacaag tgagaacata tagtatttgg ttttctgttc ctgtgttaat 189120tctcttagga ttgtgacctc aagctgcaaa cagttgctgc aaaggaaatg atttttgttc 189180ttttttatgg ctgtgtcgta tcttatggta tatatgtatc acattttctt aatccagtcc 189240accactgatg ggcacctagg ttgattctat gtctttgctg ttgtgaatag catggggatg 189300aacatatgag tgcatatgtc tttttggtag aatgattatg ttgctttgag tatgtaccca 189360gtaatgggat tgctgggtcg aattgtagct gtttaaagtt ctttgagaaa tctcccaact 189420gatttccatg gtggttgaac taacttacat tcccactcac agtgtataat tgttcccctt 189480ttctctgcag tctcaggagc aactgttgtt ttttgacttt ttaatagcca ttctgactgg 189540tgtgagattg tatctcattg tggttttgat ttgcatttat ctgatgatta gtggtggtaa 189600gcattttttc atatgtttgt tcgttgcttg tatgtcttct tttgagaagt tctgttcatg 189660gtctttactc atttttttaa attgggttat ctgttttttg cttgttgagt taagttcctt 189720atagattctg aatattagac cttagttaga tgtacagttt gcaaatattt tctcccattt 189780tctagttgtc tgtttactca gttgatagtt tgttttgctg catagaaact ttagtttaat 189840taggtctcac ttgtcagttt ttgttttcgt tgcaattgct tctggggatt tagccaaaaa 189900ttttttgcca aggccaattc tgagaaacag acacatagac caatgaaaca gaaaactcag 189960aaaaaaagct gcacatctac aatcatctaa tctctgacaa gaaaaacaag cagtggggaa 190020aggacaccct attcagaaaa tggttttgag atagctttaa attttggttg aaattggcca 190080aatggacaat tatatttgag acacttaatg actgctaaca catttgggaa ttcctcaaca 190140gtcaagtaaa gtttattgtg actttaagat tgttatccaa ttggcagaag acagtcaggc 190200tctccaaact atctaaacag acaacaatct gacttcaaat tcagtgacct taaccagtat 190260gctctgtttg caacctcgtc tcttctttct cagttgtttc atcatcatca ttttcatttt 190320catgatcttt tttaaggtct ttgtgattct tagccagtat ctttcctttc caaactctcc 190380atgcattaat gtgttcattc attcatttat tcaataggca tagataattt aacacattcc 190440atgaaagaga tatataagac aactctcctc agaattcata gcctagtgag ggggacattg 190500attgtaacag aaaatgacaa tttaaaaaaa tctaagtttg aagctcacat accaatggca 190560ataatctatg cagttcttga tttttttcta ataaagcaga ttacaatgta cttttatgta 190620aatgctcctc atgccaagct tatgagatga tagaataaaa agaagtccca aagaggttaa 190680agcaaggtca gtaagtccca ggaactgtat gtgtcctgtt taccacttgt gacttgtacc 190740tagcataggt tcccggcata tacttggcac ttaataaatt tttgactgaa tgactgaaca 190800cagctaataa atgtctaaac taggccttaa acacaggtct cctaatcttg attccagtat 190860ttattcaatt gcatccattt tctgtgcccc ctgtgatcat taccttactt tactcctcag 190920agccacctct ctcattttct tcaagtccca tctacttgat ataaatcatc ccagttaact 190980aatgattggg aagctattat tcaatatatt aacaaactta aaaacagtct cagctgaagc 191040agaggatgat tgattctcta ggtattgcac ctcagttgag tagataattt ggatcaggag 191100gcccatcaaa tcctatcaga gatataatca catattgctc ttttgtagaa tcccagcctt 191160gggagtcctg tgacaagttt tatgagaaga gtaaggggtc tgagatgtga attttttaat 191220gacaaacagc tataaatttg tttttgaaat gcttatattt ttattattaa atggttgaaa 191280ttatgaaaat agttatgatg atgtcattga tagcaggaat gaggttgata attataagaa 191340aatatgacaa ccatttactg agatccaact ctgttccagc aatgtgccaa atgatttaca 191400catgttatct caatcaaatc ctagcaattt catgactaga tatcaactgc agtttatagg 191460taacagaggc acaatggctt ccattaactc cgaatattca acaaacacaa ctcatccttc 191520acatgattca tccccaaaca gatgtactgt tcttgtttcc ttatagccac attcatccat 191580ttccctcaca cacacataat attagaatac agatattatg tatattatat gtactatata 191640tgtatactat atatacttat atatgctata agaaaggcca aactggcagt caagtaaaca 191700aaatattgaa gaccatagaa tactttccaa ctcattctat aaagctggct tctataaagc 191760tggcattact ctgataccaa aactcaaaaa agacgttaca agagtgcaaa ctacataaca 191820ttatctttca tgaagataga cacaaacatt tttgttaaat tagttaacca aatccaataa 191880tatatataaa gcataacaaa tcatgactga gtggagttat cccagaaaaa taaggttgtt 191940taacattcaa aaatcaaggt acttcatcat attaacagac tcaagaagaa aaaccacata 192000atttttgaat acatgaagaa aaaacatcga aaaaaaatct atatttattc ctgataaaat 192060ctctcggcaa actgggaatt aaagggaact tctttttcct aataaaaggc ttgaataata 192120ttctgtggct taatatcata cacaatggta aaaagctgaa tgtgttcttc tcaagactga 192180gaatgaggca aagctgtctc cttcagtctg catttactgg agattccagt caggataatg 192240aggcaagaat aagaaataaa aggaatacag atcaggcatt taaaaacgaa ctttctattc 192300acagacaact tcatcatcta tgtaaaaagt cctcaagaat ttttaaaaac ctgctagaac 192360taaccagtca acgtagcaag gctgtagaat acatggttaa aatttacaag tcaatttcat 192420ttctgtatac tagcaatgaa ctactaaaaa tttagattta gaggacaata ccatttataa 192480tagcatcaaa aatatgaaat attaatagga ataaatttcg taaaagattc acaaaaaaat 192540ggtacactga aagctacaaa acattgctga gaaacattta aaggaggcct aaataagtgg 192600agacatatac tgtgttcaca aaatgaaaaa ttaaatattg ctaagatgga aatcctctca 192660aaacttatct ataattcaag gcaatttaaa acaaaatccc agcaggcatt ttctataagc 192720tgattataaa attcataaga aaatgtcaaa aagctagagt agccaagata atttttcgaa 192780aaagaggcca aattgaagga cttatactac ttgactccaa aatttattat aaagctaaag 192840ttaaataaaa agcattgtaa tactgatata aagatagaca tatagatcaa tgaaactgaa 192900taggaattaa gaaatagtct cacatttata tggtcaactg attttcaata aaagtgccaa 192960agcaattcaa tggtgaaaga tcatctcttt aacaaagatg ctggaacata aatctataaa 193020ggctagaaaa taaaaacaaa aacatgacaa aatagaaggg tttttgcatg tttcagactg 193080agtgtaagta gtacagggct tatatgttgg ttccatgatg ttgaggacgt ggcctttttc 193140aatcttgcag ctctgaaatt ctgtacatgt acctttcatt ttgagattta aaatagtggt 193200tccaacccct tctgttatgt gtatatgata gtcagtagga aagaaagaaa agcaatgaaa 193260aacaaactct ttaatttaac ataaactgga cattgaacac atcacttctg ttatcatccc 193320actgaactga gccataacaa atataaacat atatgaaatt tcacttgaac cagttcaata 193380aatgagctgt tgattatgtt acagtttttt tcaaagatca tagaccagaa acattctcta 193440ttttgctttt catatgtaag atgggaagag ttaggcttta gattgaggag aaaatgaatg 193500gtatcctaga tcctgcaatc agagttcaaa gctgtgtaga ctttgagaag atgggaaaat 193560ttcacataat tttgtaacat tttgtaatga tgttatctac ttaagaacac cgagttacca 193620taacttcgag ttctttgatc catggccatg caggtccacc aatatttcta tgagtcagca 193680caaaatgctt agatctcaat gaaacatgag agtaattttt gatgtcatta tcagctattt 193740tctggggagt gatttttttc tttgatcata atggtgaata tttgggatca cgaaagtaca 193800aaaaatttta caggtctgca acaaaatgtg gatctctttt tctccctgaa atttttaata 193860aaagatgaag gcaatgtgct gaaagtgaaa aatgattata aacatttatc atctggggca 193920ttttgttcat tgtggagttc aaaacaaaca aataaaattt ttatttcgag ttacgttgta 193980acacgatttc ctctgagttt ttcagtctca tgaaatctaa ccttgtctaa gcttttaata 194040gaattggcta aaaaccttca ttttataagt tcctatcttc ctagttcccc tcctcttaaa 194100aaaatgtcgt cactttctgc catcctacat tttttgtctt ctcccacttt tttccctgtc 194160ttcagagaac tcacactttc tgagtacttt taatttaaag gttaatactt gccattttta 194220gagggacaag atcagtatga acagacaaaa aacctatcta taactgtcac ctgggtacta 194280ccagatttct aaaacttggg gagaaaacat ttctttaaga aaatgtgttc tgttgttccc 194340gtaagaatat ttgatcttat caaaacatgt gctccagttt ttggattgga aaacaaaaat 194400gtcactgtaa ctgtgggggg agacatttca tgcattagta tgactgagta caaaaaaaag 194460tctgacagtg aggtatgaag aaaagggtct ccaatctagt atgtttgctt gtctctctgc 194520gggtctacca gtgtgatact ttgttcagat taagcacttt tggttctcaa tctagaatga 194580taaatttgac acccataaca tcattcatgt gtatgtgtgg tatttgttat ggttaattag 194640aatttttttt ttttttttaa atggagtctc gctctgtcgc ccaggctgga gtgcagtggc 194700gcaatctcag cttactgcaa cctctgcctc ccgggttcaa gcgattctcc tccctcagcc 194760tcctgagtag ctgggattac aggcacacca ccatgcctgg ataatttttg tattttttag 194820tagagatggg gtttcaccat gttggtcagg ctggtctcaa attcctgacc tcgtgatccg 194880cctgcctcgg cctcccaaag tgctgggatt acaggcgtga gccatggccc cggccggtta 194940attagaattt tacaaccaga acgtggctca ttgacatttt ttctaaccaa tgctcgctta 195000atttgtttct tctgttttgt aaatgttgcc tctttgcaag cgtcttcagc agaaaggatg 195060gttgagataa ttttttaaca taaagttagg ttgaacagtt tttagatcat tgtgtttccc 195120taggcaaaat taatattctg aattttttaa ggtattcatt caaagctaca gtcagtggct 195180catacctgta atcccagtca cttgggagac tgaggtgaga gaatcacttg agaccagata 195240tttgaggctg cagtgagcca tcatcatgcc actgcactcc agtctggatg actgacagag 195300tgagactcca tttctagaaa aataaaataa aataaaaaat aaagtcacaa acaccatcag 195360ccatggttca ccaacctggg tgtaattggt ggaccagaga ttatggcaca tatgttgagg 195420ttgacagatt ccaccactgt caggcagcct tgtggcttta ggcagaaact gggtgtcact 195480tggggttttg cttcagagaa agccaatgag atcattttga gtgtggggtt ttcttttgga 195540gtaaaactgt agaccttcat tttcctagag aagtactttc cgttcactgt tttcagttcc 195600ttatgatatc attaaggttg tgatcatgtt gcagttgcta tttgtcagca cttattagca 195660atagtaacat gcaatgatta ctgagtgcct gctaatccca agcaatgtat tatagtaact 195720gctttgcatg aattatctca attgatcctt acattaattt tatgaaggtt gtattcttat 195780gtgttttaca gatgaataca ttgaacccat agtgggtaag tgattaagta agcatttcaa 195840atcagttctt tctgattctt aaccctctga ggtgagcgag agtgaagaaa gctaatactc 195900tttataggaa gccttcttga aacatttttc ttttcctttc ttgtaatatc ccatgtggca 195960cacgtggcct tcaaagatat tataaagact ctgcagaaaa atattttttc aagtaactat 196020tgacaggcct gggtatttca cttcagttgg gagacactgt atctacaaat tatcttatct 196080caaccttcct agcctcattt cctgcccact ccacacaaca aactgtacag ttacccaaca 196140tcttgtgaac tctaacactt gaggagcttc aaaaatactg ctgctgggag agatcttctg 196200catttactca cacagtatgt ccttttatgg ggccttctga gactcttcca ggaagaatga 196260attactcctt ccatgtaaat tcatatatag cttgattaca caatattttt catatggctt 196320tttgaaagag taatatatac ttacattaac acaataaaaa atggctttca gtgtaaagta 196380tgtctccctc tcatgcttaa agcacaggct tctcttaagg gaacaactat tacctgtgat 196440ggttaacacg cacagaaata aacacgtaca catagctctc tgggctttgc aagattggaa 196500gcctgtgtaa tttggtgttt tgcaccttgc atttttcact taatatcttg gacttctttc 196560tatattagta cataagcttt atttatagta gctctaaata ttttattata tgacatccca 196620taatttatgt aaccatcctc ctattgacat ttggattgtt tccagctgta ataaacaatg 196680cagaaataag catcctcatt tgcatgctta tacagagagt gagtggatgg gccttgaaag 196740cagatttcca ggttttgaat cctagctctg cactactagc tcctatactc ttaagctggt 196800caacctctgt atgcctcact tttctcttat gtaaaaatgg atatgtaatt gtaattgcat 196860agggctttct gagtattaag ggagatatga catgaaaaag agctttaatg cctagtatat 196920agtaaggact taatgatgtt ttactactat tgttacaaag atcatgcctt tgggggcatt 196980tgtgagtatg acttcaggat aaattcctgg aagtggaaat cttggggcaa aagacatgtg 197040catttactat tcttatagac ccctggctta attgggttca cattgaagca gatcttgaga 197100caaggatctg aatacaaata ggttatttag atggtggagg aaagtccaac aggggaatgg 197160gaaagtgaaa caggaaggga aaatagccaa taatgggtgc attattgagt cagttatcac 197220ttagtgtact tggagctcaa tcctactgag gaaactctgg gagccagtac agatgcaagc 197280atcagagtta tcctgctcca ggggagaagg agctgaggta tcagtacacc gcctcctgtc 197340ctggtgggtc agtcattggc tgagagctgc tcctaagagc agtggcatcc agattagagc 197400actgggaaat ctctgcctca ggttctggca ataatgggat tgtgtagatg attttaaaag 197460agcagaaaaa ttgaccaaaa aatcattcag atgcttattg tcatgatacg ctgggaattc 197520tctacaatct ttgttataaa ataattgtct cctcggggac accttgcctc tatgtattcc 197580acaccctggg aggtactcat ttccaacact ttcggcctat tgcacaagca gacaaagtgg 197640gtataattaa tcagaggatg aaaggaatgc aagtgccgac tattggaagc ctgtcttgca 197700tgaactgaag ggagagacaa tatggtcaga gaatcaacag tgaacctgct ccactcacac 197760tgtctacaca agctgatggc ctgatggatg tacttcttga agctctgccc gcagtagtga 197820gaatttcccc tcactactct ctttcctggc caccgctggg tggagctgtg ttgcccaagc 197880agtccgcttt cagctcacat gaaatattga gccacccatc tctgaaacag acctgggttt 197940ttaatcccct gacagctggt tatgggaact ctctgtgagc tctcaggtgt gaactgaggg 198000agacacattg gtttaacaag ttaagtaata tgtgggactt agctgccaat ttgtgtaatt 198060tagttgctcc cccggacatg ctcatgcctg atcgcaaaag taccattcat ttcatgatgt 198120gttactgatg tgcctgccca aactactgac ttggaagatc tgacagtaaa gagctcatga 198180tgagcaaccc agtcacacaa ttacttgatg tgccatgagc aggctcaatt tccactagtc 198240cagtagcata ccaaaagctg ctttttgaat ggcaaatggt tctttgaagg cttctggctt 198300tcgcatcatg tcctgattga tagtagagct tgtccattct ttttctctgt tacctatgac 198360actaacagct accagctgat gccactgtcc ttgtaattct cattatcccc taatctctaa 198420tattctgaag atattgcata agctagtgta tgcccttcat cctgtaaccc aacccatttc 198480agactaagga atgaagatga aaatgctagt ttggaccctg aaatgccttg caatgtagag 198540gtgattttcc atagctaaaa cagaaacaac agcaaaagct gttaccatat tgttaccaat 198600atttgaactt ttctaccatt tggtgagtga catttggtca ttataggcac ctcacattta 198660gtgaactgat gtcttgtgac tctaatctaa tacaacttgc taagacagga aacacttggc 198720acttccctga ggcactgatt aatgaggaaa cacttggttt tacagttaat ctgaattatg 198780caacccctgg gaacctatta attttatgag aagatagctt tatatgatga ttgcatttgt 198840taacatctgc attcataatg caaaagctac atttgtatat gaacttacct caaattaaga 198900aaatgcaatt tggagccaat ttgtggaaat gcatgtatat gtcaaatgct aactctctcc 198960ctctcccttc tttccttttg tctttccaat ggcttctaga tatgaacaga atggggaaaa 199020ccaaactaac ttgaacttac tatcccactt ttatacatat ttaattcaat ccttacaaca 199080gcattatgag tcaagtatta ttattcctat tttaaagtaa tttgcccaag atcacacaat 199140ttacaacata caaattataa aattactata tggaatttat gcctagatca attgatgcca 199200gagctcattt tttccactcc tgcaggagtt ctcaaaaact ggctgcagtt atatgactga 199260tttgggatgc tttttaaaaa taatgatgct tgaggcctac tcctagagat tctgatttaa 199320taattctgtg gtagaatgta ggcatccatg actttttcaa gttccccagg tgatactaat 199380atactgttac atctgtacca ctcttctgca ccatttccct ttactctcat aattataatt 199440cacattttgc attgtcctta cagttttcaa agttttgaaa aaatacatag ctcattgtat 199500tagtctgctt tcacgctgct gataaacacc taactgagac tgggaaattt acaaaagaaa 199560gaggcttaat ggaactcaca gttccacatg gctgcggagg cctcagaatc atggtggaag 199620gcaaggagga gcaagtgaca tcttacatcg atggtggcag gcaaagagag agcttgtaca 199680gggaacttct atttttaaac ccatcagatc ctatgagatt catccactat catgagaaca 199740gcacaggaaa gagccacccc cataattcaa tcacctccca ccaggttctt cccatgacat 199800gtggcaattg tgagagttac agttcaagat gagatttgag tggggacaca gtcaaatcat 199860gtcattccac tcctggcccc tcccaaatct cttgttctca catttcaaaa ccaatcatgc 199920cttcccaaca gtcccccaaa gtcttaactc atttcagcat taactgaaaa gtccacagtc 199980caatgtctca tctgagacaa tgagataatg atcctatgag gctgtaaaat caaaagcaag 200040ttagttgctt cctagataca atgagggtac aggcgttggg tagatacagc cactcccaat 200100gaaacaaatt ggccaaacaa agaggttaca ggccccatac aagtccgaaa tccagcaggg 200160cagtcaaatc ttaaagctcc aaagtgatat cctttgactc catgtctcac attcaggtct 200220ctgtgatgca aggggtgggt tcccatagac ttgggcagct ttgcccctgt ggatctgcag 200280ggtacagcct ccctcccagc tgctttcatg ggctggtgtt gagtgtctga ggcttttcca 200340ggtgcatggt gcaggctgtc agtggagtta tttttctggg gtgtggagga tggtggtcat 200400cttcttacag ctccactagg cagtgtccca gtagggacac tgtgtgggtg ctctgacccc 200460acgtttccct tctgcactgc agaaggttgt ccatgaatgc cccacccctg cagcaaactt 200520ctgcctgggc atccaggtgt ttccatacat cttctgaaat ctaggcagag gttcacaaac 200580cccaattctt gacctctgtg cactcccaga ctcaacacca tgtggaagct gctaaggctt 200640ggagcttgta ccctctgaaa ccacagcctg agctccatgt tagccctttt aagccacaac 200700tggagcagct gagacacagg gcaccaagtc cctaggttgc acacagcaca gggaccttag 200760gcccaaccca ggaaaccatg ttttcctcct aggcctccag gcctgtgatg agaggggctg 200820ctgtgaagac ctctgagatg ctttggagac attttcccca tcatgttggg gattaacatt 200880cactcctcac tacttataca aatttctgca gccaacttga atttctcctc agaaaatggg 200940attttctttt ctactgtatt gtcaggctgc aacttttctg aacttttatg ctctgcttcc 201000cttacaaaac tgaatgcctt taacagcacc caagtcacat cttgaatgct ttgctgctta 201060gaaatttctt ccaccagata ccctaaatca tcctcctcaa gttcaaagtt ccacaaatct 201120ctagggcagg ggaaaaatgt agccagtctc tttgctaaaa cataacaaga gtcacatttg 201180ctccagttcc caaaaagttc ctcatctcca tctgagacca cctcagcctg gactttatcg 201240ttcatatcac tatcagtgtt tttctcaaag ccatttaaca agtctctagg aagttccaca 201300ctattccaca ttttctgtct tcttcagagc cctccaaact gttccaacct ctccacctgt 201360tacccagttg caaagtcact tccacatttt caggtatctt ttcagcaact ctactggtac 201420caattactgt attagtctgt tttcacacta ctgataaaga catactgctg ataaagacat 201480agagactggg aaatttacaa aataaagaga tttaattggt ctcacagttc catgtggctg 201540gggaggcctc agaatcatgg cagaaggcaa ggaggagcaa gtcacatctt acattgatgg 201600cgggcaggca aagagagcat gtgttcaggg aaactccagt tttaaaacca tcagatctca 201660tgagactcat tcactatcac aggaacaatg aaggaaagat ccaccctcat aattcaatca 201720cctcccactg ggttctttcc acaacacatg ggaattgtgg gagttacaat tcaagatgag 201780ttttggctgg gaacacagca aaaccacatc actcatctaa tctttaaacc aaccgaggga 201840agaattttta tgctcatttt acagaagaga aaactgagat tcaaggagtt tgaaatctta 201900cccagtatta caagagtaag tagctgaaac ccaggataat tggaaaaggg ctgtttttat 201960ccttatttag aataccgtaa ttgtgatccc ttttggttcc ctctctcttt ttccctcctc 202020ctctggttta ttccccagtc tgggcctgca aaagactctt ggatgccatt taaagctgtc 202080aaccatgtat gagtgggaaa agtggtagct ctgaggactg attcattacc aaagaagcat 202140gggttcgtag tgaaaacagg gagatagaga tagaagtttg aattaactta agaaggtgat 202200tttatttctt tgaagcccag tttcctcaac aataaatgga aaaagaaacc ataatagcta 202260cctattgttt tgtcatgaga ataaaataaa attatctgta caaagatttg taaatcgaaa 202320ggcattatat ctatgttagt cattactatc tcacttataa tgattagttt tgaggtgtta 202380tgttgctttt ccagggctgt tgtaataaat taccataact ttgatgactt aaaactaccc 202440aaaactattt tcttccagtt ctggagacca gaagcctaaa attaaggtga cagtgggggt 202500cacatccctt ctgaaggctc tagaagagaa ttcattcctt gcctcttcca gcttctgttg 202560gctgttggca ttccttggtg ttccttggct tgtagtcaca ttacttcaat ctctgccttc 202620gtcttcccaa caccttcccc tctgtgtctg tctcaaatct cctgctgctt tctttatgag 202680ggcacttgtt actggatttt ggacccagct gaataatcct agatgatctt ttcatctcaa 202740gatcttaact attatatatg caaagtttct ttttccaaat aagctaactt ttacaggttc 202800tgggaattag gacatgaggc atatcttttg gggggccagc attcaacccg ctacaggtat 202860tatgagtgtt tctcttttct taggctcaat atccccctct ctgattcact aactgccact 202920tgctagtgga tttcagttcc aaatctttga tttggtaaat aagttgtcca gtacataagt 202980cagctctgcc tcctgcccta accaactaca cagaactttc tggtgttttc tgaatatccg 203040ttgtactttc ctaccctgtg caattccttt gatgcctttc ctctctttct ggaactcaac 203100actgttttcc accctcttct gtgaagtatt tctaatcatt aagaaagact gctctctctt 203160ttctctccaa atctgttaga tttttgggct ttctgtcttg attccagcac ttatcacagt 203220gtgaccttta ctgttgtagc tgagcacctg tctgcttctc catgaaactg agtgttgttt 203280ttctcatgtc ttcaagatac agatagtgac tcattcatct ttataccacc tactgcctat 203340ggccagttgc cagacatgtg gtaccttttc agcagaggtt ggtgaacctc atggaaccgt 203400gtagaactgc atggcacctg acaacctcct ccctcaggtt tacctggtct actaccctgt 203460ggaggcagct gaaataaaag ctgtagagtc ttattgcctt ttcagtggtg taaattctac 203520tgataacttt gtcttattct ccctatttgg acatttcttt attattatta ttgtggtggc 203580agtaattatt gttattttta aaaattgact tataggtatc ttttcttcct tttttaagga 203640aggttttctt ctgtgaaaat atattttttt cgggtggaga gtctatacct tcactgtcag 203700taataactga tatttcggat atcatcttac tttgcttaac tcttggttga ggtaggcgaa 203760ggtacagaga agctgtttga gtctctattc tctcctcaaa tctctgccct gagctttgat 203820ttaggctgat atttttgaag tttaataaac tggtttttct ctttactttc ccgtatcctt 203880tttgtagtga aatccttact ccatcttatg tttaaaatcc aaaactatgt ttgggataaa 203940gtaaacagaa gattgaattt tgtctcattt tctaaacatt accaatgctg tatgtggtag 204000gtacacagtt gccttcatga tttttcaaaa gcttttcctt ttgaagcgaa cacctgtctc 204060agaatctcct agattaaata gttatactca catattcctt aaactgtaaa ttctttgaag 204120gtaggtactg cctttgctct tcacaataat catggtgcca gtgcagcatc tggcacataa 204180ttggtattcc aaaaaggtgg ataaatcagt taatacaaat aactattctg ggagttggca 204240caacatcaat tgacaatgga ttcctctgat ttcttatttc tactataata caacttaatt 204300tcgtggtagt atgttaatgg acataatctc tctttttacc aacacaatat ccagggaagc 204360ccaaataaac tcttggttga aaccaacata tttcacaatt cctgttcttc atatccaacc 204420tccacataac agctgatgat gacatgttgt cttacagctc ctttagtccc actctgcgaa 204480tttagggaca gcttagattt gtgagcattt gcggaacaag acagaagcct gccccactcc 204540aaaagcagtt aactttagtc cacatacaac agtgtcccaa gtttcaggcc taccacttaa 204600acctctaaac tcagaatgag atctctgggg atcagccagg agactaaggg gttttctctt 204660cctgtttttc ctatgggctt ggacatcagt cttaccacat acggcatggt ttctcccagg 204720agaagtccat ttgaacctca ctaaacaata atttctgggc catacccttg cagtatgtct 204780aagacactcc tatctgtttc ctcctactgg tccatcatac atacttgcaa taaataatca 204840aatagcagga caatcaagtc ccgaccctca ctgctttgta acttgggctt ggagaggttt 204900atccaagata aattggttca tgcattatga tcacctccca gggcatgtgt gacattcttc 204960atagagtaca ctaagttctg tataatgggg gtgctctaca gaaggctgtg ctttcctctc 205020tcattcctct tctgccttca aaatgaaata tagagctgaa aggccatacc tgtcttaatg 205080acgtgccaag ctgcatacat tacattttgt atctcacctt ggacaagaaa gaaataggat 205140ctttaaagaa atgctcaaca cactgtgttt acaacctctt tccgctctaa attcatcatt 205200tgtatattaa tataactatg atataaaatt gccaacaaaa gagatataaa agcatactga 205260aatagtctct aggagttcaa tctacatttc ccatcactca gagccagaag cactcaatca 205320tttgttcctg catgtaaaag aggctgacac tcagaatgag aatacaaagc agctgtcctt 205380caaggtccag ccctattgta tacatagtaa gaacacccct tccccccaac tccaacaacc 205440tgaccactga cagagtagga aacacggtca tgataggagc tgggggcttt gcagtcaccc 205500agcaaagaaa taggggcagt cgtctttaag ataataaatt aaagttaaat catttagtaa 205560aataagacaa aaataaagat aaaagtaaag gcaatctgaa acaagctttg tatcaaaaca 205620acaacaacaa catgctgaag aaaataagga agaaaattgg tagctttagg ttttttagtt 205680caagcttgca cctctgtggg aaaggctagt gtcactgaag tgtggctgag gtgttttcag 205740aatccttata actcacaggt tgcttctgac atattcaaaa ttcctaccat tacagtcatt 205800gcatccagaa agtctagtga agtattgaag agtctaatac tgagtcattg aaggatatag 205860tacaattaca ccctatgaca attggtgtga acttttatct attttctctc ctaatctctt 205920caccaggctt tttatttgct tatctccctt agttccagta cttaggattt ttatgggggt 205980tattgtaaca aacttacaaa ctgatttctg tttctactct agtcccctgc aacttaccct 206040tcacacaact actagcataa aacccaaata tagtttgttc cctccctaaa actcttgaaa 206100agctccttaa gagtaaaatc ctgcagagta aaatccaagc agaagcataa aagcaccata 206160taatctggct catgccgatt tcctctaacc cctcactctc tcagaactcc ttgcaattgc 206220ttcaaaacac attagtgatt catgcttcta tgtcttctct catgagattt cctgttagcc 206280tgggatactc ttctcctctt ctaaggctga tcaattttca ttcatgcttt gagactcagt 206340ctgggtgcca tttcctttgg gaagccttct ccttttcctg gagtgaatta tatatatgca 206400ccacaagtta ccatagtgtt tttttctccc tccaacacta ctccttacat tgtatcacaa 206460ctgtatgtgt aattgtctct attccccact agacagtaaa cagtaggaaa tcatggactg 206520aatctccagc acccagtaac ataatgctag gaacagaaca gatgtttaat aaacattcct 206580ctaatgaatt cacaagctgt tcctgagaca gattctacct ggagcatttt ggcatatatt 206640atgcctttta atctttcagc aacaaatcca atcctttcct tttatatgag aatagtccat 206700ttcttggagc attcttcaag catcaggcta aggtaggtga gtattattac accaggcaat 206760tgaaggaaag agaggatgct cagatgggag gtgagtgatg aaagaggcaa tagttaagaa 206820agaggaacat aaaacagaga tcttaagaca ttacaacttt gatgttataa gaaaattgta 206880tctccttttt tgagatttta gagggaaaaa ggaacatgag attatatcac tgtatattat 206940taactaggta atatgttcac tattttatat gcttttatgc attaacttac ttaagcctca 207000aaaaactcca gaatataaat actattatta ccctcatttc acagaggaga aaactgagga 207060acagagaaag aactaacttt tccaaggtac atagctagta agtggcccag tgatgattat 207120cagcctgagc agtctgaaac caagagactg tgctcatgac cactacacta tgttctttct 207180gcatgatgca gttacagtgc agaggggtaa atattttaac aagttgtagc agcagcactg 207240atggaagact ggtggtcaag gaaggcattc gagaggagat gaggaataat gaagtcttag 207300aggagcagga attaaccaaa gggcagggag tatatgagca ttttaggaag aaggagtgat 207360gtctgtagag atgagcaggc tgtggagaag aatctaccac cttgatctgg agttgtagtt 207420atggctgaat accaaagaaa aggggaaagg agaaaaacag gaagaccaag atggacatca 207480gtgtgtgttg ctcaagttca tactcagtag aattggcctg ggttgcccag cagggatgta 207540ggagaagtcc ttcttgataa gaccacagtt cccctcacat gaggatgctg ggcaacaagg 207600ctcaaatgga caattcccag agccagcaaa ggactgggat gcataggaga cactaaatac 207660acactattaa ggtaaaaaat atccatagag accttctttc caattaccca ctccccaacc 207720ctcacttttt tttttaaatc agtgaggacc acatgttcaa atacattaag tgatatgttc 207780aaatagatta agtagtatag ctccttggca gaaaggttgg gactagaaac aaggaaatgc 207840agcctttttc tttgaagaac tgatgatatt ttagggagat acaaatcaaa caaaaacatg 207900ttgaagtcaa taacagcaca gcatctcaat aaatgcaaaa caaacaaaca aacttatttc 207960acaatttaag ttctaggtgc tgaagggttg actgagtaaa ggtggaaacc aggaaacatt 208020cctggagcag gtgagctttt aaccaggttt ttgtgaagtg atggaccttg gctggaggca 208080aagaggactg gaagaaatga gctcctgaag gggagacatg tcttatgata ctatgatata 208140tgccagtttg gtgggttgta ccggcaggca gcttgataga atggaaacag cagacacgtg 208200ggagtcataa agaactgagg tcaagttgct gacattcatc agctacagga ccatcagctg 208260tggttcaatt atttgatccc atgtgttctc ctctattcaa cgagggtaga ggttctcatc 208320acttagtggt tattgtagtt acagagtcat ttggtgacag cagggattga tatccttaga 208380actcctcttt tttactattc attgaatgaa ttcacaaatg aataaatgag tgaatggatt 208440gccggttaga ttttcttcaa gctaaagaac acttcaaccc cttcaagcaa tggaaatgtg 208500acaccagtgc attctagcat aatcagataa aacatacctc actgtcccta acctcctctt 208560atgtagcttg cctgggagga caaaggaaag gggaacgggg tctcaagatc gctgagtggt 208620gggctgtttg ataatacaaa tataaaaagg aagaggcatt ccacagacct caggcactta 208680tccctaagct tctgagaaac accgttcctc atcggatcca tgggtggagg cacatctaac 208740ttacttttac atcctataca aggagtgcct agagttttac tcctactcaa actacaggga 208800aagaaatcca gcgtccgcat ttagagtatc aagccatatt gtgggatatt ttctgaggtt 208860tgggtctgaa acacctaaat gttgtgcttg tttgtactct gctatgtcaa ggagagtctg 208920aaaagctcca gtcctggagg aacactaacg cctagtcaaa aaattagaaa agagctgttg 208980cagtgtaaaa tatcataaaa tcttagggtt ggatgggacc ttgcagccac ccctgataag 209040ggttttcaga ctctgtgatt ttaagaacta gtagaatttc aaaaagcaaa acaaataaaa 209100acaaaaataa gaatttgacc taagaaggct gtattagctt cctagggctg ctgtaacaat 209160ttaccacaag ctgggaggct gaatggaaat ttactgtctc acagctccag aggctgtaag 209220tttgagatca aggagtcagc atggctggtc tctactgagg actgggaggg ataatctgtt 209280ccaatgcttc tcttagcttc tggtagtttt ctggcattcc taggcatttc ttgacctgta 209340gatggcatta tccctgtgtg ttcacatcat cttcctcttt gtatacatct gtctctgtgt 209400ccaaatttcc cccctttgat aaggacacca gtcttgaatt aaggcccacc ctaatgtcct 209460cctcgaaact tgatcatttg caaaaccact atttccaaat aaggccacat tcatagatac 209520tacggattag gacttcaata tctgttgtgg ggacacaagt taactcttaa cagtagccaa 209580ctagtaattt aattaagtaa ggacatttga aaaaaagaga aaatacatgc atcctactat 209640cttcatcatt ttgaagaaaa gagattttaa ctcctaaaat tttaggaatg ttatcatttc 209700actgcaaagg gcagtctttc ccagaaagag tagctggaat cttacaccag acaacatgca 209760catatgcact gcagcactct tcttcctaat cttactccag gttgtcagag acattgccac 209820caaccactag ggatctgtga aaaagtttta tttggttctc ctggtctagt acaactgttt 209880gtccactgct taaacctctt ttgcaaccac cttagcctga gctttgaaac tatagttctt 209940acatgtgata acagacacaa tcaaaacaag agcttatgct gttaccttaa catcacactt 210000ctgtacctaa aattatgtca gaccaaattc ctttcctgtt ctctgaaatt tcttcagcaa 210060gtatgctatg ggttgtttct gtgaaacaga aacttgtttt tgtgaaatct cacatcaggg 210120actgagccag gcattctaaa gcatctttat ttatttttat tttttgcagc atctggagtc 210180tgaatttcag ctctgtgtct gttcacttgg tttacaaagt gatgctgatt taatcagctc 210240ttacactgga ttttatgtaa atgattttgt agttgtatac catgaaaagt aaccactatt 210300caaaggcaat gagtaatcat aggcagccac ttaaattttg ggactacttt tcttttattt 210360gtagccaaag acaagtttcc tcaagctggc agaatactaa ccacatgaag cttggattgt 210420ttgagttgct attcatgacc agcaactgac tttgccttat aaatgtccag agatcctgat 210480acacaaagag tgtggactat cacttgtcaa atattttgag aaaataagaa aagaatgagt 210540aaaaattgtg gcttaaatat tatttgtaat ttctcctatt tgtcatttct tttgttaaat 210600gctatgctct tgctcctggt gctgggggga actcagtatt gagatgtcaa taatcaattg 210660aaatcaataa tcaattgaga gtcatttggt gacagcaggg attgatatcc ttagaactcc 210720attatatttg aatcatcaaa cagcccacca ctcagtgaga tgtcaagaga aaacctttga 210780gagaaaacta agggaatgct aagaaagtca tgctgtaggt gaagagattg gattttggtg 210840tcagatatag gtggtttaac cccccaactt tgccattcag ctttgtgtga tttcaggcac 210900aatacttaaa ctccctgggt attagtttta tctcctatac aatggaatta acgtctactc 210960cttaagactg ttataaggat tcacatacct ctactggaga tagggtggga tgagtgggga 211020gaggactttc aagaagcaaa cagtaaacta aatatacttt tgcctaaagg tgaggcttga 211080tcaaattcca caggaattgt tatccataat agcacttcat taaaaatatt ctaattttct 211140tccaggagtt caataattat gtaacatact ttttgggaaa aaaaatgagt ttgcctccag 211200caagagtaga agaaaaccaa atacgcttca taccagacaa ccaaagtgtt ctgaaatata 211260ataaactatc caaggtcatg tagaaaacta ggcattcaat gtagttttct ggcagacttg 211320caggctcaaa atgctatttg gtaaacagag ttaaaatgat gattgtcaca aaacttcata 211380agaaaacaaa tttatcaggc actctttttt tttttttttt ttttttttga gatggagttt 211440tgttcttgtt tcccagactg gagtgcaatg gcatgatctc ggctcactgc aacctccgcc 211500caccaggttc aagtgattct cctgcctcag cctcccaagt agctgggatt acaggtgccc 211560accaccacgc ccggctaatt ttttgtattt ttagtagaga tgtagtttca ccacgttggc 211620caggctggtc tagaaatcct gaatttaggt gatccaccca ccttggcctc ccaaagagct 211680gggattactg gcgtgagcca ccatgtccca cctatcaggc actttaaaag attatttttc 211740attctgacca aaaccaaatt gtggttctta acatttaata attattttgt aataaggctg 211800ttatgtgtag attaaatttt ttattttatt tttaataaat attgttggat ttaagaaaat 211860gttgaatggg gtatggagga tatagaagat gtaattaaaa tatcaaggaa gcaacaaaca 211920aatgaatttt ctctgttgct cttattccta gcagcaatag actttggaac atttgcatac 211980gttcatcttg gcagtagttt tcaaaggaat tttttcctga cttattttaa cactttgtgt 212040aataaaatcc tataaattcg cagaaaagtc ctgactattc agggaagagt tcttactcat 212100gtaaaaggca tttcacattt ctgcatgaac actgacatat gaacttaaaa gctagaattt 212160aacttaaaca atggtttgtc tgtgctcttg tcacctagtt ggataaggca gatgctgggg 212220aaagtcctgg tgatgtagcc tcctcagctc ctcaggaatc acaaggaaag acatgcagaa 212280aaaagaccct actaccattc ccaggacatt ggccttgtcc tgatgtcttt ctccacgcca 212340cagtcctata gctgaaacaa gaggcggtgt cctgtgggag gaggcagcag gctgttctag 212400aattaaatct aagcattggc acttagctgc atgactttag gcaagtgtct cagactcttt 212460aagcatcagc ttctcatctg taccatggag ataccagtat ctaccccaca tggattatta 212520gaaaattagg ctagatggta caaggtgcct ggcacataat gggtaaatta tcagcttcta 212580acctcaaacc acacagttat ggcatattgc atgaggagaa tgaggatctg agaaaagctg 212640gtaaactaga ttccatttgt tccttgttta gttctagttt gtactcaata gtaaggaaac 212700gttttttttt tagttggaca ttgtttctgc tttccaagag gcaatcacct actcacagaa 212760agcacataga tcagatatta tttaatatgt attacaggat ctgctgtaga tgcagtatta 212820ttttacgtgc taggaagata tgaaggtgaa gtaagaaatg ttcctactct tttttttctt 212880tctttttttt tttttttttt gaggagtctt gctctgtcac ccaggctgga gtgcagtggc 212940acaatcttgg ctcactgcaa cttctgcctc ctgggttcaa gcaattctcc tgcctcagcc 213000tcctgagtag ctgggactac aggcatggcc caccacaccc agctaatttt tgtattttta 213060gtagagacgg ggtttcacca tgttggccag gctggtctca aactcctgac ctcaaatgat 213120ccacccacct cagcctccca aagtgctggg attacaggtg tgagccacct cctaaggagg 213180ttacaatatg tagaggaaga agacagggga tgaggatatg ttactccttg aatagctagt 213240acaatctatc tatgaacact ataagcacac tttcattcct aacagttttt ctgtatatgc 213300ttctcatttg acactagtca tacctggtgc cagctgtgtt aaatacatgt caacagatgg 213360cagtcaccat ttactattca ttctgtgcca agcactgtgc tgagcacttt gaacacctta 213420tcttgtttaa accttagaag gaacatgtga ggtagggtta tcgtcaacat tttacatgcg 213480gaaactcaag ctcagagagg ttaatgaata tgccccaaag caacagctca taagtgatag 213540aattgggttt gaaatgcagg tctgtcttcc tccaaagctt tctttccccc tgaaactcca 213600tattttacaa cctcacctct gtgtattttt cacaagaggt tacatagtag gtgctccaaa 213660gtatttattc ctagataatt ataaagaggt aagtagctgg tctaggaagc cctttcaaca 213720ttaactcagc cctgttgtgg atttcccctt tcagctgagt gagatttcat agtaaagatt 213780ttgtttccgt ataacattac tacctctaag tgcaatttca aaggtctttt cctaagactc 213840atggcacatg tctctcatac atggttttcc atattatata aagcatttat taggaccttt 213900taaggtctcc atcaatgatc ttaaattggc agaattttgt aatccttcct tccttagctt 213960atgactaaga ttacaagaaa gtccatgcta tttttataat tgttgttaga gaagaagaat 214020gctatgcaag cctgcaagct ctaaaacaag cagactgtat caagaacaca gctcctgccc 214080tggagggtat ctgatctaaa accacaaatg ggtgtattat atgacactga ccatttccat 214140acccttgggg cagcagtaaa gatcatttga tgtcctccta ttttaaaaat ggacaaattt 214200ttcttttttt ttttcttaag atagactttt accaattata gtgaaagagg ggaagaatag 214260aatagcaaga actgagaaac agagcagtgc taggagtgtt gagcctttaa agttcattga 214320aatgtttcta gctgcttcat taatatccaa ctgtaggtct gaggatggca tctgtagttt 214380gtgagtgggt gtgtttacac caaatgggaa ttcaggaaat gcattccaaa atcaaataga 214440aataacttgg aaattattgt gtctttattt tttcattaaa caatccttaa ttgagtgcct 214500gtgtgatgac tgatagtccc tgctgctgga agctcatggt ctaaggccag agacaacaaa 214560ggcactgctg gttgacacag aatggggtga gttttatgac agtggaattg aaggttatag 214620ttgaaatcca aaggagtgcc acttaataca gccaggtagt gagtgattta ggaaaaccca 214680gcctggagaa gctgaagctg agttttgcac gttgagtaaa agcaactgca ctcaagaatc 214740aggttccaga gaggacagca tgtcaatagg tatgaagaaa gtaagagggc ttattatgtt 214800taaggtactt aaggaacaaa acaaacaaaa attcctataa atatataact gggttctcct 214860gcaggtaact ctagagataa aaccagaggg aaaaaccata gccatatctt gaaggacttt 214920ttatgccagc cttaggagtt tggactttgt cctaagaaga gtgattaaaa gatttttaat 214980ataaacatga taaaaaccaa tttcccttat agcaccatga aggataggtc agggagggct 215040gtgtgtgaga acaggctgtt acagtaagaa aacatggtgt ctgtggagat aaatatttaa 215100gtgctaaaac tgataagaca aagtaactgg ttaggataag gttagacgaa ccatgcagct 215160gagtggatga ttgtgccgtt taccaagata acatacacaa gaaagaattt ggaaggggtg 215220atacttaaag tagattgtag cataagttaa tggaccagta ttttgtatta tagttgtttt 215280gatgtccact tttttttcta tcatcctcca ccagtttaca gaggttagaa gatagatttg 215340ggggtcattt caaatttcac atcacttatt ggctttctga tcttaagagg atcacttaat 215400tctataagcc tcagtgtttt cagtggtaaa atgataagaa tacttatggt acaggggtgt 215460cagaaataag ggttaaaaga gaaaatgata tagagtcctg aaacaaaata agtgttgtta 215520aataaatggc agttgttatt attacttttg ttgctgtctg ttgaatgctc actgagtgga 215580gggtctatgc ttccttcact ctgtgtttct gcaggacctt gtctagtggt ctgtgtataa 215640aagactaagc cgtaatcatt gaattgagct gaattgaagt ggtacaacat ggaaatgcca 215700aatgaagttt ctgaaagaca tgaagtgttt atgcatgttt aatgtacttc agttaaataa 215760gtcactcaga aagggtcaag agtaacattt ggagatgaag gacaggaaaa gagcaatgat 215820attaattaag caagatcgga agtggctcct aacagcaaaa aaaaaaaaag taaactgtcc 215880caagggaaga agcctaagaa caagtcctca cagacatgtg gggctttcaa gagaagtcag 215940tgatctgatg gttctcctca gctcaaggtt ttacctagat gtcatatcag caattatcta 216000acatattgac cactgcaaga agaaaactat agatcataag ccatatgaaa agaattaaac 216060actagcctag tgtagaaacc taatctgaac attgatgtat tgtccaattg tcatcatgat 216120tcttatttta tttttctttt ggtcatttaa ttttctctga atgtgagcca tggtaatatt 216180tatatgtaat tttaggttgc ctttgcattt tatactaaat ttgtccaaca gagaaatcaa 216240aattatattt atcattaaca taaaataaat aaaacataag ccaacaaaaa cagagaacaa 216300tctttttttt tttttttttt tttgagatgg agtctcgctc tgtcgcccag gctggagtgc 216360agtggcgtga tctcggctca ctgcaagctc tgcctcctgg gttcatgcca ttctcctgcc 216420tcagcctccc gagtagctgg gactacaggc acccgccacc acgcctggct aattttttgt 216480atttttagta gagacggggt ttcaccgtgt tagccaggat ggtcttgatc tcctgaactc 216540gtgatccacc cacctcggcc tcccaacaga gcacaatctt aagtggaaat ccgattgagg 216600tactggtgct gatttagatt tgaggggtta atttgcctgt cgctaaagag caagttagca 216660tatatctagg catagtaggt aaatctaagc caatggggca tgaaaaggga ggaaatattt 216720aacaccacat aaattcagct gttcttacgt gctttctttt ctaaaaaacc tactacactc 216780ctgctgtgca ctatggccca ccctatctca gggctgctgc aaattgttat gcacattatt 216840cataccacga gggtgccagg cagaggagag ggaaggcgaa tcccaaagaa acaggcatct 216900ttttctaatt tgcacaaatc tatggctaaa caacagcaca actacatctt atgccccaca 216960gtcttttcca tcagaaaaaa aaaatcccca aattcacaaa ccaaagccta gtttcttagc 217020acagattgtg ctttttttca catctcggat gaatttaaaa gcctgagatg ggaggcagaa 217080cagggagaaa gcaggttaaa ggaaacatag ttgctgttat gtaggataaa taagtctaga 217140gatctaaatt atagcatggg gactatggtt aatgatattg tgtactggaa atttgctaag 217200acagatttta tgggctctta acaaacacac acacacaaag gtaactatgt gaaatgatag 217260atacattaat ttgcttgaat gtagtaatta ttttactatg caaatgtgta tcaaaacatc 217320atgttgtata acttaaatat atgcaatttt taaaatacct gggaggctga aaaaaatagg 217380atctacatag atacattttg atgttcctga acttccctaa ccctctcttt aattgtctcc 217440agaagcctat tatttatcct tttaaatggt ggtttttcag agtctttgga tctgcccgaa 217500ttcataaaag gccatcactt tgatgcccct tcacgattgt gaggtgccta ttgcaacaca 217560accatgactg ctacgtctgc taagagggct ttttgagtct gttctctgct cctggaaact 217620cagcttggag aaaagccagg agaaagcttg ctagtgtaca tggtactctt atgctgattt 217680tcaaatagtc aactcccaca gctttgcatg ctttaacacc aaaatgaaag acagaaacag 217740tgttattgct tcattgcttt ctggtcagga acaaacacat atgaagctgc ctttacccat 217800acctattttg agttactcag aaagatctaa atagaatctt atatccaaac ataatggaag 217860aaagaaaata tgtttctact ctttgctctt gtttacatag tggtctgcag tttgggtctt 217920aatatgagtt ttggaatatt atgaaacagc ataaccaccc agctagaaca taatagctaa 217980tataaatcag ccagctcaca agcaaaacag atttttaaaa tctgctttat attctctaat 218040gcaccacaga gaaaatagca agaatttgaa atttatatac agagaaaaat tatttagaga 218100ctttgttgaa aaataagtat atataggagc aataaacaaa tctatcatat tagaacctag 218160aattgcacta aaaggaagca gatttcagtt atttcccatg ccataaaaaa aatatcaaaa 218220actatagatt tagaaacctg agccttcctc attccaccaa aaatggggga ggcaggaatg 218280ttaccaacag gacaaattac acacatttgg gttgaaagaa gagaaagaac tgtaataagc 218340ataaccagca acatgcagaa aatcagcaga agagcttgga gatcaaagca gaccataacc 218400taaagctaaa gcctttaaca gactatacca agtcatgcat gaaaagtgcc aggaaggaat 218460gtatttacta aattatccac ttagtcccct tgagatagca gtattctgta gaatttcaat 218520ggaaatgcaa caagatccaa agtgttatta gagtccatat aaatgtcctt tcccctctca 218580gctgttctca agccctcacc attagtactt tgaggggctc gtaaccccac tcagtaagct 218640ttttcttcat gcccaaagga aaaattatag acttctcttc caagacttct agatgtttca 218700ttattgatcc caaactattt ccctggtttc atcttcagga ccctccttct cacatcccct 218760atgttgcaac ttattctgtc tctagtgtta acttattctt ccttctaagc agtgaggaga 218820agtatttaag aactgggtca atggggtcaa acaaaccttg atccaaggac cagctctgcc 218880atataccaaa agtatgacct tggcaagtca cttcattcat tcattcatga attcaaccat 218940atttattgag catccagtgt gtgccaggat ctgtgcttag ccctggagat aaagaacagt 219000acaaaaattc ctgtcctcat gagacgctta ttctagttag gggagacaga aaatgtctaa 219060taattgccat gatgaaaact aaagcagagt aaagatgcag agagtgatgt gggagtgaag 219120agaagagaga gaggaaagtg gttgtgcttt cgacagattg atcaaggaag gcttttctga 219180taagatgaca tttagatgga aacctgaatg aatgagaaag tgagccttgt ggtatctggg 219240agatagtgtc ccaggcagag ggatgatgag tttgtggtct gatgatttca gtctcttctt 219300taggaactac tctccattgg tattgtatcc ctgttttggt cagtttggga agctataaca 219360agataccaca gactaggtgg cttaaacaac agaaatttgt ttctcacagt tctggaggcc 219420ggcaagttca ggatcagggt gccagcagat tcggtgtctg gtgagtgccc tctttttggt 219480ttgcacatgt cagtcttctc attgtatcct caaatggtgg agaaaacaga gaagggaagc 219540aagctctctc ttgtctcttc ttaaaagggc attaatccca ttatgtgggt ctcaccttca 219600tgacccagtt atctcccaaa gctctatctc caaatgtcag cacactgggg attagggttt 219660caacatatga atttggtggg taggtatggg ggagacaaat atgcagtctt tagcaatctc 219720tgatcacatg aagttgttgt gaggatcata tgaggtttat tagtataaaa gtacttaggg 219780tcattcctga agcataagaa atggtcacaa aaggacagta ttattttttc tatcaggttc 219840agcaagaata tcccctttga aagctccttt gccatatgct tcctcccatc ctcattgccg 219900gcattattcc tctctccatt tggtttgcat tgcatgttgt acacaactgt aatagcattt 219960atcatattgt atatctttat tcttttattt tccctttttt ttgtttcctc agctagactg 220020tgagcttggt gaagggagtg actgcacctt attcttgggc cctcagagcc tagcacagtg 220080cctgacacat taaagtttct aaattcatcc ttgctgaatg aatgaatgag cttaaatact 220140tcttgttcca tgaagttttt aaacttattc tcatctctat ctccacttat attttgtatc 220200agtcatatta cataatatat gtaccactta acccatattg ttttgtatgc aaaatttttt 220260gcatctatat cttgtttcca actagatcat aaagtcttat ggaacagaaa tggtatatta 220320tattctttgt atctttctta gagtgttatg tacatacatg ggttaattat cattgtactg 220380ttaaaagaaa agcttaatga ataaatgcac agatggactt aaaaaccatt tgatggagta 220440taacacattt tttatggatg gagtgagtaa tgatgttaga atataactac tattgcttca 220500gagcaaccac agagaacctc agaagtccaa ctcagagaag ctttagaatg ctctcagaat 220560gaagtggaac aataaacatc tcaatattat ttcaaaactc aaaaacttat tcctggtcac 220620taaaagaagt aaaaagagtc taattcttta gaaacagaca aattttggct cttgcatttt 220680caggaaaatg atcttggaat agttagacaa agtgccaaaa atttacagaa ttcagtggga 220740tgtgatttta acatctagaa gagttgtgtc ccaaataaag gaagcataca acaaatatag 220800aggattcccc tcatgttcct atttacaata atctaggaac gtgatggagt tcctttacat 220860agaaagacac ctaaatatct cagccagaaa atgatgcaaa attctatagc agactgtata 220920gggtttccaa tctctgtaag tgaacagaat tacacatgta tacactgaat ttaagggaca 220980cacatgataa gaccacccca gttagagagt gtattaattt tctattgctt cctaacaaat 221040caccataaat ttagtgattt aaagcaacac agttttacta tctcacaatt tctgtgcatg 221100aatctgggta tgcattagct tggtcttcag ctcagggtct cactaggctg aaatcaaagt 221160gtcagcctag gctgtggtct catttgaggc ttgagatcat cttctaaggt cagcaggaaa 221220gtggtgatgc tgctgctgct cttgctcctg cttatctctt ttaaggtttt gccagattaa 221280atcaaaccca cccaggaact tttccttttc aattaactca aaatcagttg actaggaaac 221340ttaattgcat ctggaaaata cctttggcaa tataatgtaa cataatcatg ggagttatta 221400cccatgatat tcccagcttc tgctcacact caaggggaga tgattataca gcatgtgtat 221460accagggggt ggatatcttg gaggctatca gaattttgtc taattcagag ggcactgaga 221520cagataaata agaatcaggg tgtcccacac aacagcatta cacataagtg tctcagaatt 221580acttaggatg gtaaaattac tgaggatgat ataattgtgt tctgatcctt ttttgtttgt 221640ttgtttgttt tgagatggag tcttgctctc tcacccagac tggagtgcag tggagtgatt 221700tccgctcact gcaaccttca cctcctccac tcactgcaat cttcgcctcc tgggttcaag 221760tgattctcct gcctcagcct cccaagtagc tggaattgca ggcatgcgcc acaagcctgg 221820ctaatttttg catttttagt agagatgagg tttcaccatg tttgccaggc tggtcttgaa 221880cacctgacct caagtaaccc acccaccttg gcctcccaaa gagctgggat tacaggcatg 221940agccaacgtg cctggccatg ttctgatcgt ttaatgatag caacatttag tattatagag 222000catgaaaatg tcaaagtcgc aactctaaaa tgtttttatt tagaaatatg tgcctattag 222060aattttagtt actagaatta tttaagaaaa ttggacgttt tataataaca ggtcaacatt 222120gtaattccaa gctaaaatgg gtgagtaatt gctttaggct tgtaatttca gattaaaatc 222180ttacttgtaa catttaagag aacttcaggt tcatcatacc tataaattta atttattagg 222240cttataagag tgacttaagt tcttgggaag tttcttaagt cacctaattg gtatacttca 222300aaagaaaaca gaaatatgtt acattaataa atgtgcttaa atatttaagg aatttaatta 222360agttgtgaaa tcttccttca aagtaattat aaaatttgct atgtttatga atgtgttaat 222420aagatttata aactaaccta aaagcttaag aagaacaaga gtttaaataa attgaatact 222480aattttgtac caaaattacg ttaaataggt ttttttctag gcataaaaac tgccctcagg 222540aacataaaaa tactcatgtt caacaagttt caactgtgct attttgtatg acctctgtca 222600agttacttaa cttctcttag cttcagtttc ctcacctaca aaatgggtat catcatatta 222660acttttaggg ttgctgtaag gattgagata ttttgcataa agttgttgga acatgtaagg 222720ccctcaataa tgtcagctca aagaaaaaga gattagaggg tccaagatga tagactagaa 222780gcagctcacg tgtgctgctc tcagaaagga aacaaaaggg ctagtgaaca ctgaccctgc 222840aggccaaaca tctgagaaac cacattggga tccatcaagg cagcagggga cacagagcag 222900agagaagcaa agctggacac cagcctatct tggcttagtg tgaaaccagg agaacctctc 222960ccacatggta aagggtgagc gagtgagagc cccctagaag attcgcactc tccacaggga 223020cctatgcaag actgggaatg gaaaaatccc tctggccccc cacgctccca tctgtgataa 223080taaactgagg tagagagcca cctggtcatt ttgtggaggc aacttttgag tccaagaggg 223140cctctgcaag cctttggccc caaagcagac cagcactgtc accatagctc caatagaggc 223200cacatttgca gtgtctggga gaaataagat tgatctatcc tcatttcatt ggacagggct 223260cactaccagc ttccagccca gtggtgctgc ttctgcctga acttggccag cagccatagc 223320ctcctgctgt cccaggaagc acccagatgg cagaacaagt gatctcaccc atccccacca 223380ctgataacca ggtggacaat gcctgttaga gcttccagcc gaacagtccc acttctacat 223440aaactcagcc aagagtgcag cattctgttg tcctgagaaa tacccagatg gtagggtggg 223500tgacaccaca cacccatgcc aatggtagcc aagggggaca atgcctgcta gatcttcaag 223560cccagaaaac cctcttctgc ctgaactcag ctagcaggca aagcctgcct ttttcctgag 223620aaacactgag atggcaagaa gggtgaccct accaacccct accactggaa gccaagtggg 223680taatgcttgc cagagtttcc aattcacagg ttccacctct gtctaaattt gttggaggtg 223740cagcttcctg ttgccctgga aacacccaga tggcagggtg ggcaattcca cctacccctg 223800cctctcatag caagatgggc cacatctgct agagcttcca gctcagtggt tctgcttttg 223860cctaaactct gtagacaggt gcaaccttat gtttccctga gaagcactca aacaacatat 223920tagggccaac ccagcaagga aatggcttgt ctgctaactg caacctctgc ctaaggaagt 223980cctgtggacc agaacaccca acaaaagaaa cacagtcatg gagacagtaa ttggaggggt 224040ctcctccaag acccaggagc agactataat tgaagccagt tgtctaatcc cactgagaat 224100cacaatcaaa ccctcaaggg caccaaaaaa gataaaagca aaaagcccat ccaaaggact 224160gcaacttcaa aggctgaaga aacatgagcc cacattgatg agacaaaaac agtgcaggaa 224220ctctggcaac aacaacaaca acaaaaaagg caaaaatgtc ttcttacctc caaatgacca 224280cactagttcc ccagcaaagg ttgttaacct ggctgcaatg accaaaatgt cagaactagc 224340attcagaata cggatagaaa tgaagatcat tgatattcag gataaagttg aatcctaatc 224400catggaatct aaagaatgta ataaaacaat acaggagatg aaagataaaa tggccatttt 224460aagaaagaat caaactgaac taacagaggt gaaaaactca ctttaagaat ttcataatac 224520aaccagtatt aacagctcca tcaaccaagc tgaggaaaga atctcagagc ttgagagatt 224580gcttctctga aataactccg tcagacaaaa ataaagcaaa aacaataaag aatggataaa 224640acctctgaga aatatgggat tatgtaaaga gaccaagaga ccaaatctat aattcattgg 224700catcccaagg aaagggagag aaagcaagca acttggaaaa catatttgag gttatgatcc 224760ataaaaattt tcccaatctc actagagagg ccaacattta aatccaggaa atgcagagaa 224820cccctacaag acactatata agacaaccat ccccaagaca catagtcgtc agattctcca 224880acgttgaaat gcaggaaaaa atgttaaagg cagctagaga gaaggggaag gtcacctaca 224940aagggaaccc tatcaagcta acagtggaac tttcagcaga aactctacaa gccagaagag 225000acttaggggc ctatattcac cattcttttt tttttttttc tttttttttt gacagagtct 225060tgctctttca ctcaggctgg agtgaagtgg cacaatctca gctcactgca acctccgcct 225120cctgggttca aaagattctc ctgcatcagc ctcctgaata gctgggatta caggtgcccg 225180ccatcatgcc tggctaattt ttttattttt agtagagatg gggtttcacc atgttggcca 225240ggctggtctc gaactcctga cctcaggtga ttcacccacc tcggcctcct aaagtgctag 225300ggttacaggc ctgagctgcc tcgcccagcc tcagcattct taaaaaaaaa aaaaagaaaa 225360aagaaattcc aaccaagaat ttcatatcca gccaaactag tttcataagc aaaggagaaa 225420taatatcatt ttcagacaag caaatgctaa gggaatttgt tactgccttg cacagtcatt 225480aagggagtgc taaatatgga aaggaaagac tgttaccagc caccacaaaa gcacacttga 225540gcatataggt cgttgacact ataaagcaac cacacaatca agtctgcata ataaccagct 225600aacaatatga ttacaggata aaacctacac atatcaattt tttttttttg agagggaatc 225660tcactgtcac caggctgaag tgcagtggta caatcttggc tcacttcaac ttctgcctcc 225720caggttcaag tattctcctg cctcagcctc ccaagtagct gggactgcag gtacgcacca 225780ccacgcccag ctaattttta tatttttagt agagacaagg ttccaccatg ttggccagga 225840tggtctcaat ctcttgacct catgatccac ccactcggcc tcccaaagtg ctgggattgc 225900aagtgtgagc tactgcacct ggccacacat atcaatatca accttaaatg caaacagaat 225960aaatacctca attaaaaggc acagagtgac aaggtgaaga agaaagcaag atccaactgt 226020atgctgtctt caagagatcc atctcacaga caatgacacc catagattca aagtaaagga 226080acagagaaaa atttaccaag caaatggaaa acagaaaata gcaggggtaa ctattttaat 226140ttcagacaaa atagacacta aaccaataat gatcaaaaaa gaaagaggga ggctagacgt 226200tgtggctcac ccctataatc ccagcacttt aggaggccaa ggtgggatga tcattagagt 226260ttgggagttc gaccatcagc ctggccaaca tgggaaaact ccatctctac taaaaataca 226320aaaattagcc agatgctgtg gctcacactt gtaatcccag ctactcagga agctgaggca 226380caagaatagc ttgaacctgg gaggcagagg ttgcagtgag ctgagaccat gccactgcac 226440tccagcctgg gtgacagagt gagactctgt ctcaaaacaa aacaaacaaa aaacaaaaag 226500aaggacagaa gggcattaca taattacaaa gagttcaatc caacaagaag accttactat 226560cctacatata tatccaccca acacaggtgc acccaaattc ataaagcaaa tacttacaga 226620cctatgaaga gatttagata accacacaac aatactagga gattttaaca ctccatgacc 226680atattaacca tatcattgag gcaaaaatac taacaaagat attcaagatg tggacacaac 226740atttgaccaa atggactgaa cagacatcta cagaacacta taccccaaaa cagaatatac 226800atttttccta tctgcacata gcacatactc taaaatcaac cactcaatag gacataaagc 226860aattctcagc aaaccaaaaa acaaacagaa atcataccaa ctacattcac ggactacagc 226920acaataaaaa tataaatcaa tactaagaaa atcactcaaa acaatacaat tgtatggaaa 226980ttaaacaacc tgcttctgaa tgacttttgt gtaaataatg aaaggaaggc agagatgaag 227040aaattctttt aaagtaataa gaaaaaagat acaatatacc agaatctctg ggacatagct 227100aaggaagtgt taagggggaa gtttatggca ttaaacgctc atataaaaat gttagaaaga 227160actcatatta ataactacca tcacaattag agaaactaga gaaacaagag caaaccaacc 227220ccaaagccag caaaggacaa gaaataacca aaattagagc aaaatgaaag gaaattgaga 227280tgcaaaataa aatacacaaa agatcaatga ctccaggagt tggtcttttg aaaaaattat 227340taagacagac catgagctag actaataaag aaaaaaggaa agaagattca aataaacaca 227400atcagaaatg accaggggta ctttaccact gatcccacgg aaacacaaaa acacctcaaa 227460gattatgatg aacacctcta tgcatacaaa ctagaaaatc tagaagaaat ggatgaattc 227520ctggaaatat ataacctccc aagattgaac taggaagaaa ctgaatccct gaacagacca 227580ataacatgtt tggaaattga atcagtaata aaaagcctac caaccagaag aagcccagaa 227640ccagacggat tcacagccga attctaccag acttacaaac ctatgacatc cataggtttg 227700taatgaagaa ctggtaccaa ttctattgaa ctattccaaa aaactgaggg ggagggactc 227760ctccctaact cattctatga ggccaacatg atcctaatac caaaacctgg cagacacaca 227820acaaaaaaag aaaacttcag gccagtgtcc ttgatgaaca tagataaaaa atcttcaatg 227880aaatactagc aaaccaaatc cagcagcaca acaaaaccta atccactatg atgtagtagg 227940ctttatccct gggatgcaag gttagttaaa catacacaaa ttaaaacgtg atttaccaca 228000taaacagagc taaaaccaaa aataacatga tcatcttaat aggtacaaaa aagctctcca 228060ataaaagtca gcatcccttc atgctaaaaa ccctcaacaa acttggcatt ggaggaacat 228120atttcaaaat aaaagccatc tatgacaaac gcatagccaa caccatattc aaggggcaaa 228180agctggaagc atttcccttg agaaccggaa caagacaaag atatctactc tcaccacccc 228240tatttaagat agtactggaa gtcctagaca gagcagtcag gcaagataaa gaaatacaag 228300gcatccaaat aggaagagaa gaagtcaaac tatatcagtt tgtagacgat atgattctat 228360acctgaaaaa acccatagtc tcttcccaag agctcttaga tctgataaaa aacttcagca 228420aagtttcagg atacaaagtc aatgtacaga aatcagtagc atttctatac accaacaatg 228480tccaagctga gagccaaatc aagaatgcaa tcccattcac aacaccacaa aaagaataaa 228540atatctagga atacagctaa ccagggagtg aaaaatctcc ataatgagaa ttacaaaaca 228600ctgctcaaag aaatcagaga agacacaaac aaatgtaaaa acattccatg cttgtggata 228660gggagaatca atattgttaa aatgaccaca cttcccaaag caatttacag tttcaatgca 228720atttctatca aactaccaat gaaattcttc acagaattgg aaaaaattgt ttcaaaattc 228780atatggagcc aaaaaagagc ttgaatggcc aaggccattc taagcaaaaa gtacaaagct 228840ggaggcatca cgttacccaa cttcaaacta tactacaagg ctacagtaat caaaagggca 228900gggtgctgat acaaaaacag acacatagac caatagaaca gaatagaaag cccggaagta 228960aagccacaga cctacaggca tcttgttttt gacaaagttg acaaaaacaa gtaatgggga 229020aaggactccc tattcaataa atggtgctgg gataacttgc tggccatacg cagatgattg 229080aatgtggacc ccttctttat accatataca aaaatcaact caagatggat taaaggctta 229140aatgtaaaac ccacaacatg aaaaccctgg aagataacct aggaaacatc attctggaca 229200taggctctgg cagatagttt atgaagacac caaaaacaat tgcaacaaaa acaaaactgg 229260acaaatattt tctcccattc tctagtttat ctgtttattc aattgatagt ttctcacagc 229320aaaagaaact atcaattgac taaacagaca acctagagaa tgggagaaaa tgtttgcaaa 229380ctacacatct aacaaaggtc taatatccag aatctacagg gaacttaaac acattaataa 229440gcaaaaaaaa aaaaaaccca ttaaaaagta ggcatggaca tgaacagact gtgtccaaaa 229500gaagacatac acatggccaa caagcatatt ataaaatgtt caacatcact aaccagcaga 229560gaaaggcaaa tcaagaccac aatgaaatac catctatacc agtcagaatg gctcttacta 229620aaatgtcaaa aaacaacagg tgctggcaag gttgtggaga aaaggaaacg tttatacact 229680gctggcggga atgtaaatta gttcagccat tgtggaaagc actgcagtga ttccccaaag 229740aacttaaaac agaattatca atcaacccag caatcccatt attgagtatg tgcccaaagg 229800aatataaatc attctaccat aaagactcat gggtgcgtat gtctattgca gcactatatt 229860cagtagcaaa gacatggaat caacctaaat gcccatggat ggatatacaa catattacac 229920agccataaaa agaactagat catgtccttt acagcaatat ggataaagct ggaggccata 229980atcctaagca aactaatgca gaaaaagaaa cccaaatgcc acatcctaac ttataagtgg 230040gagctaaaca ctgggtacac atgaccataa agaaaggaat atcaggcact gtgtcctact 230100tgaggctgga gggtgggagg aaggagagat ttgaaaaatt ccttatcagg tactctgttg 230160attatctgga tgatgaaata atctgtacac caaacctctg tgatgtgcaa tttacctgta 230220taacaaacct acacatgtac ctctaaacct aaaataaatt taaaaaataa aaagaagaaa 230280aaagaaattg tattcttctc ctaaaacaag gacacaggcc ttttttacag tctgtccatg 230340ccactttaaa agttcaatcc tccatatact tgcttaagta ctgattagtt tgttaccttt 230400ctaggcacaa atgatgttta gagaattaat tttaatgtca acagcaggac agtaacctgt 230460caaaccccag ggaggttgat cacacccttc agatcttaac cagaaagtgg ctgaaggaaa 230520gccattgcaa acccatgtct catttttgtc cctaaaattg agacatagaa ggcagaaagt 230580cagcatcagt ctccaaggac tttttgcaac cagtatttct gtgccaacat cttaggccaa 230640attattggaa tgaggtttaa aacactaggg gaagggagaa gctgaatatt ccccctaggc 230700taatagttgc ctggtgaaat gtcactgcat ttgtgtcaga atgcgcagag gaatgcaatc 230760ctgtctgggc agaaggcaaa tagaaaaccc aaaggagagc tgattcaatc actctgtatg 230820atttagaatg tattatggtc aacatagaca ctgatctact tttcccaagt tcctatattt 230880agggactgaa actgctgttt caaataaaaa aaaaacataa aatgaattat tctgaacact 230940tatacaaatc tgttagtgtg ttcaatttct cctgggaaaa tgcaatgatt tgatgacagt 231000tctggaaggg aacaaacaga agtatagaaa ggggtatggg gaagtttcag agagagagac 231060agagacagaa ggagagacat aaagagatag aaaggagaaa tacagaaaca gagtctcaaa 231120gaaagaaaac aaagaagggc agattcagag acaagttgtg aggcaagaac cagacagaga 231180aaacaaacac aaagaagtaa tatttagaac acagagaggc ctaacactga aaaccaatga 231240cataaaaaca aatgaaacac tgaaaaaatg tatataactt ttaaaaaccc aaggaaccag 231300ccagaggagc tggggaggga aaagagagaa aggaagataa cccatggcaa cacgcaattg 231360agtcaatatt tattgagtgc ccacagacag catggcaacc tactggaact taagtagaaa 231420taaattcaat tctctccggt cctgaagggt ggggaagctc aggtctgcca ttctgcctag 231480gggctgagga agtgtgagta catgcagttc tgcactgagc tctgcttgct gcagtttggc 231540cccagctcca cttccttggt ctgacctgca accttcttcc ccaaattcca ttgaagaaaa 231600acaaatactt aaagagacac aaagttttgt ttgtttgttt gtttgtatgt ttgttttttg 231660gtatttgtaa catcaggtct aaaacaagta ctattattag taataatgct atatttcttt 231720atgaaacata aaaatctgga agctagatgt tttcaaaaca gaaaggttaa aattttaaga 231780agagaccaaa caagagacca ctttcattac gtatcttccc tcccaggtcc tgacatatat 231840aataatacat agtattttaa aagcaccaaa accccctcct ggccaacatg gtgaaaccct 231900gtctctgcta aagatacaaa aaattagcca ggcatgctgg tgcgtgcctg taatcccagc 231960tactcaggag gctgaagcag gagaatctct tgaactcggg aggcagaggt tgcagtgagc 232020cgagatagtg ccattgcact ccagcctggg tgacagggca agactctgtc tcaaaaaaaa 232080aaaaaaagca cctcacatat cgccactaaa gaacttactc atgtaaccaa acaccacctg 232140ttcccccaaa aaacctatga aaattaaaat aaaataaaca aaacaaatct ctctaaattt 232200tatacaattt agtctagaaa atacgttgta agaatgtcag agtacacaga cacatatctg 232260atcaaaatgt ctccaaaaaa cctgcagaaa gggaaatgta gtctattata tgactcaccg 232320tgcccatgaa ataaaaataa accatggttt ctaagaaata caaccatttc tggcagtggg 232380tgctagaaaa tagatttgca aagaaaagac acaataatca aagctcatta tgttccatca 232440atggaccata cctattcaga ccaaactata cctggtaaac ctaaagaaag aaggcacata 232500gcagaataat ttgtggaata ttagcttatt tattcttcct atattttcct acctcacaac 232560tggaatttta gaactgctct gcctctagga tttttacagg gtggaaaacc aaaagacact 232620taaaagagat tttttttttg tcatattaga acttcttgaa aagactatgc tactattatc 232680ctgaggatgc aggatagaga gaatactttc agaagcttgg aaagagggag ctatgcctcc 232740tgaaccaaaa agagggtcaa agtcaagggc ccaggcattt ctcagtagca aaataccacc 232800ccagggaggt gttatgtgtt agtggaaagg atcacacagt ctggaacaga gagattctta 232860atggtaccca gtggacaaag gaccaatgac tacatctcag atgatgtggc actccctata 232920actaacttta ggaattcagc acaactctat ggtgtggcaa gtcccagtag tgacttcaac 232980tgaattctct accactctag caaaatagac tatgagaaca atttttgtta attttataaa 233040aaatgagcta tttcatatac attggagttt gtggtctaga tgtcatatat gctacacaaa 233100ccttggcgtc aaatagaatg aggtctgact taacttttgg tcatcttact taatttctcc 233160aagtcttact tctgtcatct gtaaatggag accaaaggta gaattttatt catttgttat 233220tataagttgc gagcaatagc tgtatgctca gtgcttagtc cagtacctgg aacatagtca 233280gtgttcagta atggtcatta tttttattca gtttgaaaaa tgtctcactc tagtaagaag 233340tcaagcaaaa ttttgacaca aagcttatac gtaactatta cagagggaaa caccatcagt 233400tatgcacaac tgtttacaag atttaaaaat gtagactatt aatagttctt caagagaaat 233460gcccctaacc atttcaggaa ggacaatatg aacatacaaa ccaagaaaaa caccgaccat 233520ctcccacaac tttctcagca gaggcccgtg cccattcaga tcaacgtgtc caataaacct 233580caaactcatg ggaaagaaaa attttaaaag caattcaaag ctttaatatg tcaatggctt 233640ttaaaaagtc aactgtcaca cttcttctcc ttttcttccc tcccttccct caacacaatc 233700gtgtttgagg ccaaagctat ttataattct tggagtgact gacgttaaaa gacttttgac 233760aagcagtcta gtgaccttga tgaaagtatc ttaatctgac agatagactt ctcaaatgga 233820atgtgggagg ggagagcata ctttaacttt tggtaagcta aaatagcacc ttgaaaattg 233880aatgacccta gatatggatt tagtagttgt acccatttca ggttataaaa gccctaagat 233940taaaaaaaaa ttgcaagtcc agctcaggat agaggaaggt tgatatttct gcatctaatg 234000tatgcctcta gctcattagg taataaggtt tgcattcaca ttaaaattct tagtgtgact 234060tcaggctctc ctcatttttg ctttcctcta cccttctcaa atgatttggg actaatcatt 234120ggtttcagtt ctctcaccaa ggggcccatg gtgaaaaaat catgacttaa caattcaaca 234180agcatttctt gagcatttgt tatgaactca ataccaggaa taccgacatg gacatagtct 234240ctgctgtcag ccctatctca aaactctggc agaggcttct ccagttatat agtttggata 234300tttgtccctg ctcaaatctg atgttgaatt gtaatctcca gtgttggaga tggggcctga 234360tgtgaggtat ttggttcatc caggcagatc catcatggct tggtggtgtc ctcatgatgg 234420tgagcaagtt ctcatgagat ctggttgctg cgaagtgtgg cacctaccca ccccctactg 234480tctctcttgc tcttgctttt gttatgtgaa gtgccttctc ccacttcatc ttccaccatg 234540agtaaaagct ccctgaggcc tccccaaaag ccaaacagat gccagaacca tgcttgtgtg 234600acctgcagaa ccatggacca actgaacctt tttttaaaat aaattaccca gtctcaggta 234660tttctttaga gtaatgcaac aatggcctaa tacagaaaat tgatatttag aagtgaggca 234720ttgctataaa gatagatacc caaaaatgtg gaagcagctt tggaactagg tgacaagaag 234780atgttagaag agtttcgaca tctcagaaga agacgacagg aagatgaggt aaacttcgca 234840aatttttaga aacccattaa atggttgtaa ccaaaatgcc gatagtgata tggacaataa 234900aatccaggct gaggagattt cagactgaaa tgaggaatta ttgggacctg gagcaaaggt 234960cacccttgtt attccttagc aaagaatttg gctgtattgt atccatgcct taggaatctg 235020tggaagtttg aacttgagag tgacaaccta aggtatctag attgaaggaa gacatttcta 235080agcagcaaat tgttcaagat gtggtctgag ttcttctaac agcctatgtt cagataccag 235140agcaaagaaa tgacttaaaa ttgaatttat atttaaaagg aaagcagagc ataaatgttt 235200agaagatttc tcagcctagc catgtggcag ggaaagaaaa agcatttttc tggagaggaa 235260tccaagcagc ctgcagagaa accacttgct agagaaattt gcataaataa aagggagcca 235320agtgctaata gccgagacaa taggaaaaca attttgaaag catttcagag acttttgaga 235380cagcccatca caggatcaaa gacctaggaa ggaagaatga ttttgtgggc caggcccagg 235440gctctgctgc cctgcacaac ctcaggacaa tgctccttgc atcctggctg ctccagcact 235500agccatggct caaaggggcc caggagtagc tcaggaagca gctccagagg gtgcaagcca 235560taagtgttgg tggcttccac atggtgttaa gcctgcaggt atacagagtg caagagtggt 235620ggaggcttgg caccctccac ctaaatttca gggaacatgt agaaaagcct ggatgtccaa 235680gctgcttatc agagtgagcc ctcacagaga acctctattc aagtagtgca gagaggaaat 235740gtggcatagg agtccccaca cagaatcccc accagggcat tgcctagtgc agctgtggga 235800agagggccac catcctccag acccaagaat ggaagaacca ctggcacttt gcaccctcag 235860tgtagaagag ccacagacat gcaactccaa cccatgacag caaccacagg gaccgaaccc 235920tgcgaagcta caggccagag ctgccaaagg ccttgggagc tcacaccttg caccactgtg 235980tccagcatat ggggcatgga gtcaaatgag actattttgg agctataaga tttattgact 236040gccctgctgg gtttcaaact tgcatagagc ctgtagcctt tttcttttgg ccaattttcc 236100ccctttggaa tgggaatgtt tacccaatgc ttgtactcac attgtatctt ggaagtaaat 236160aaattatttt gactttacag gcttataggt ggaaggaact tatctccaga tgaaactttg 236220gacttagact ttagacttgg aacttttgaa ttaatgccag agcaagttaa gatttggggg 236280tactattgag aagtcatgac tgtatttcgc agcatgagaa ggatgtgaga tttaggggtg 236340gagaagaggg gtggaatgat atagtttgga tatttgtcca tgcccaaatc tcatgttgaa 236400ttgtattccc caatgtagga gatggggcct gatggaaggt gtttttgtca tgcatggagg 236460caggtcccca ggacttggtg cagttctcat gatagtgagt gagttcttgc aaaatttggt 236520tgttgtaaag tgtgatacca cccctgcccc gacacacata cagtctctct tgctcttgct 236580tttgccatat gaagtgcctt ctgttttgcc ttccgttatg agtaaagcat gtacaagcat 236640ggcactggaa tgtactgcag gctgtaatac gtgagactgg ctcatttatt taaaaaaaga 236700ggtttaattg gctcatggtt cggcaggctt taaaacatgg cactggcatc tgcttggctt 236760ctggggaacc agcttctggg aaccatgaac ggcctaatac acccagcttc ttatagctgg 236820gcccattcat tcttgtctac cacccaatct cttgagccac ttcctgtggc tcccacaacc 236880tcccactatg gcattgtatt tttctttctt gtacagttac agagcagcta taattcaaga 236940tttatgaact tttgaggttc attcttctga tgatgattgg aactcatgca tgagtttcca 237000ctaatgttcc attttataga aaagggcttc catttttatt ttggcttcca ttttcattct 237060tgttctagaa gtcttcatga ttttctagat ccttcctacg aagagtttcg tttcactcag 237120acagtcctgc ctctctggag ataaggcttt attatccaga gcaaaatctt agtggctgtg 237180atctttctac ctgcacacaa gcttccctaa tgacatctgt ttggatttct atgttctatt 237240tgtacattac tttgtgccca cctccaggag aactcagtgt gagcttgtaa ataataaaca 237300gacactgtgt catattctaa agctatggag ataagacaca gaacctacaa tcaacaacta 237360cagtctcaaa gggaaaacaa taaggcaata gacaattata agtgatttat aactataagt 237420agcagaagta ttcagaaaca ccatgggcat agagaggaaa agcccaagtc ttctcatgaa 237480agtgaaggtg ggcatcacag aggaggtgag gtttgagtgg aattgtaaag gtgacatagg 237540agtatactag atgaactaaa tggttgatag gaaaaacggg actgggtgaa ggtacacaga 237600ttggaggtag tggggacagt gtatgcaaaa gcctgggata tacaacagca tggtatatct 237660aaggtataat aaccctgtat gttttgctta ggagtttgac ttttatctta taggccaaaa 237720cagcaaataa aattaatctg gagggcctac agactcctgc ctctcatggt atttagtaat 237780gtctattgtg gatgaggaag acaggactgt gaatcaaatg ctgtctgttt gcaatctaag 237840catagataaa cttgagccac tgaaggattt tattaatctg taaataatgt acctgcaagt 237900ttctgtcttg tgactccaag taggagagct tccttaagat ctagcctggc cttgtggatg 237960tatttactga ccatgtcagg atttttatct ttccagactt ttttttttct ttttagatga 238020agtcttactc tgtcacccag gctggagtgc aatggcacca tcttggctca ctgcaacctc 238080tgcctcccag gttcaagcaa ttctcccacc tcaccctccc aagtagctgg gactacaggc 238140gcctgccacc acacctggct aatttgtttt tgtattttta gtagagatgg ggcttcacca 238200tgttggccag gctggtctcg aattcctgac ctcaagtgat ctgcccgcct cggcctccca 238260acatgctggg attacaggtg tgagccaccg caactggccc agacattttt aattgcaagt 238320aaacagcctg tagatttctt tgaaggtttt aatatttgcc ctcaaagtga gaatattaaa 238380ctatggaaga ggaataagca aacaaataaa tatgaatagt gtgataagtg ccataataaa 238440ggtacattct taaaagcctg caacataatg cagagagaaa aactctacct ggatgattag 238500agaactcttt atcaagtaga taatacaaaa gccttgcatg acaagttaga actcttagag 238560gtggagaagg gaatttgcag aagaggaaac agcctaagct aagagcacta gtgtgggggc 238620atctgtggtc tattcaggaa acagcagaga attttgtatt gacagtgtgg tggtaaacca 238680ggactggtgg cagatataca actgtaaagg caggttgagg caattgtgaa aaaccttgta 238740taacatgctt tgagcagagc tgtttcttag acacacctct gatgtggtaa ggagaacagg 238800ctgtaactta gcatggagac agagagagta gtttaaaatc cagtaaggca ttcggagcag 238860agaagctgat ccagaagtaa gagcatgcac cccctatccc gctgcctgga aaaaatgcag 238920aagtccagat tggagttaca gttccatcac aaatggccta tgattcaggc tgtgggaaca 238980cctcaagcaa tctcagcctg atagagccgc cagtgccctc tgatggtggc ctgtttgatg 239040gccagaaatc aaggcaccat ctaattcctc ttccatttcc caaggtttta ccaaatgctt 239100ggctgctgtg ataactgctg gagccacaga tcttcaagac attggtcttt caatcgaaaa 239160gctcatagtc tggtcatcct gatgtaagac acagagatga gcatatcatt ggtctatgca 239220ataaaaaaat atgcataaga aagttgtata gaaggatgat taactcagga ctcgggggct 239280ttgatggtgg tttgatctct gggttggtag tgaaaatata cccatggaag atgtaattgg 239340cctctgaaat aagattagga cttcagaagg taattaatag gaaggctttc attgagacca 239400acatgggtta tttggaaaac tgaaagtaag tctgtgttct agaagcatca acagttggca 239460cagattttgc agtggctctt ctgaggaagt cttaatgata ctcaatgttt cctacagggc 239520ttcactcagc aaatatgtat taggcatctt ctacaggcca agccctctgg tgagcaatgt 239580gatatgggac ccccaaggtg cttacagcga agcattaaga cgggcgttca gacagaaaga 239640tatgaagggg tactttcttt aaagttcaag ttgtacacaa gtagcagttg gcagcaaagg 239700gaaacaggct tttacatgga agggggcagt caaggaaagc ttcccagaga aggaagtgct 239760tgagttgaac tttgaagaag aaatgagtag gaatttcccg gatgacagtc caggaaaggt 239820gtggcaattt tgtttctgtg atgtggctct agatgagtgt cttcctgcct ctctagtcat 239880gtcatctctg ctcttctctt caggctcctc ttcttcccac ctctactgtg attgatcctt 239940caaggttctg tgctcagccc ttccgttggt tcttggattt gtcctttccc gccatgtctt 240000catcacatcc acaacttcca ctaacacttg cgagcttcaa acttcttagt aagtaaagac 240060tccacattgc caagtacagg actttttacc caggaagtca gcctatctct aatgaaacat 240120gctccaaaaa taacttacca tctttcatct ctgttgtccc acctatcttt ttgtggcctg 240180attttataag tcaagaaccc tgaagagatc cttagcttgc ttcctttcta ttcccaacat 240240ccagtgactc tccaagttct gcagtgctct taaatccttt ctgtgtccac tgttaattcc 240300ttaaaggggt ccctcataat ctcttgcctg tctgattgca gtcacttcct aatctgtctc 240360tctatttcca gctcacactc taaacctatc cttcctcact actgctgggg tggtattccc 240420aatcagcaag tctgattttg gatcatatcc cacttcacca cacccctact gcttttgata 240480gtcccatgtt cctgacaacc tttggaatta ggtctcaatg ccttagggtg gtagacaaac 240540ctctcctgct gacctcatca gtagctttat ctgttgtcct ctcccttcag gaattcacca 240600gaaattctaa acggattgta gatctcccaa aatatcattt tttttcatat tttcttgcct 240660tgaaatgcta tcacctgtaa tgttttccct atcactcttt ccacatgaca ctgtcacgtc 240720cccttccttg actctgcctt ctggatagat ggccactttc ttttctgggc tacttctgga 240780ccaggtattc tgaactatct attattatgc cactttgtga aaatcaaaga agcaatatag 240840cttaggagtt aggaaaaagc aaccaaatcc cagctctgac acttaccagt gtgaacttag 240900gcaagttatt taatttctcc ttggcttaat ttctacaaaa cttagctata aaatgggact 240960gataatagta tctgcctcat aaaattgttg tccagttaat gtatataaag tgctttaaac 241020aaaaaagtac tgggcttata gtaaggatta tataagaatt tgatgattgc caactgtgag 241080catctcgatt tgagggaact atgtcttgtt catctccatg ttcccagggc ccagcaccag 241140tgtcttacac acagggtatg tggaacaaat gtttgtttac agaggaatga ctgaatctct 241200ggcttttcct ttcccttggt gtaagaccca gtaaatccct gagtcatgct gattttcctt 241260tccaatgtcc tccgtgttta atttttttct atggtaacta tcacagctcc ttcatttatt 241320cagcaaaaag ttattagtca acagtgtgca aggcacccta attgctagag ataactgctg 241380taaacaagac agattccttc tgcttatgga cattgcattc tagcagagga cacagacatg 241440aaataacagc acattaatta cctgatcaag agtgatatat ataataggag tcttggggag 241500gtcagaaaat ttctcttaga taaaaaattt gactaatata aaaaaaaact agaagtaaat 241560agctagaata aggtggtaaa agctgtggtg gagagtgacc caggcaaagg gaatggcatg 241620tgtaaagctc tgaggtagga agaaatttaa catgtagatt tttagttccc tgtgtctgga 241680ttattgctac aactgctacc cgatttctgt aaagaatgat cgtccccact gctctcatat 241740taaactttcc atcatgtaga ttccctgctc aagaaccttc catgattctc tattgcctac 241800tgaatgacat gctaattcct gagttgtttt tcatgtccca atataatatg ggtttcagtg 241860attatccaat tttatcttcc acaaatcatc taccatatac tcaccatagc catcatgaac 241920tacttgatat tctccacaca ttctttatgt tttcctacct ttctaccttc gttcaggttg 241980ttattgctac caaaatgcct ttcttctctg ttcctatcca tgcttgtttc acaaaaaaaa 242040aaaatccatt tgctatatat gtgatgtgct ttgatatttt ctattctagt ctattacatt 242100ctagttcagt ttttaagaca aacaagcaag atgtaagcca cataagtgat ttcgtaactc 242160atgaatggat cttgacccag ggttgaaaac attgtcctaa atagctttaa agattttttc 242220atgatctatg attttgaaga gggttggctt tactagagtc aaaattttct tcttgcagta 242280gaatcctcta accagctaca tctggattat tagtcatctg tattctctga agacaggaca 242340gtgaaattca gcctgaacac atgatcaatg gggaagctaa tcatacagat ccaaagcacc 242400ctaaggatgc ttagtaatac atcttgattt gtaggtgata tcacactttg ctatttcaat 242460cctgccaatg atctgaactg aagttgctaa ttgtattgaa ctgttccgga tcacatcttg 242520ggcaaagacg aacatgggag agcatttgtt ccagctgaga ctgagaaaaa aaaaatcctc 242580ttttagcaaa taaatccata gcaactagat gcaactgtag ctcatgttat ggcccatcat 242640ctctttctct aagacagaga acctgggcat cggccgcccc cttctttaaa taacctacca 242700ccaatcctct tgactttaca tcctacaagt gtctccgatc tgttgacctc tctccaactt 242760tcctgccttg cctggagctc aagcctccta aatgtttatt ttgcaatctt tcttagtgtt 242820tcacacagtg aaagatctat tgtgccatcc tcctgctaag aaccattcag ttatccgctg 242880tttgttccct taggagaggg tccagtggat actacataaa attgcttgca aagcccttcc 242940tgatccatcc tcagtaagct ttcctgtctc attcttactc ccttctttct tcttttcctc 243000ctccttgtct tctcttccgc cattcatctt ctaccctctg atcattaaaa acaaaaagct 243060ttgttcctca aacacttcat gtttgttttc tttggtttgg ttttctggtc tagagcattc 243120ccttctttat tagacagctc ttatccttcc ttcaaaacac atcttggata tttcattcct 243180tgagctttct aagtcaaagc catgagactc tcccctgtcc tcccttaatt ccctatagtt 243240caccattaca ccatatccaa attatccatt cacttcactt caacagtgtg aaagcaggac 243300tttttcagca caatatttct attgcctatc acataatagg agaaccctgc agtgtccagc 243360taagctgatg acttgaagat aaatctgcct aaccctggga tgaagtatct gtgaactatt 243420ttgacagcag atgtaagtca tgatctccaa agaacttacc agttactatt aatatttttt 243480tttacttagt atgtttaaat ctccatttgt tttatgtatc tcatttcata aattcaggag 243540tgggtgaaaa aaagaggtgc taattttggt tccctgaaat tccaatattt caggtaatct 243600ctcttagaca ctaaatttta cttcttacca ggcccaccct ggccaatata tagatcctca 243660gattaatggg gctttcacaa cagttttatt caattattct caatcatcca cttttcattg 243720attaaacaac tactaagtgc tagatactgg aaatactaga tggttaagat gtcatccctc 243780ccctcaagac ctagtctatc ctgagctcca aggagggaag ggaccaaaca gtaagctatt 243840atgaaataga agttcttaca taaactttaa cagccaaaga gttccacaga agcataaaaa 243900tgttaagaaa tgagagtttg ctttccgtac aaaaaaagaa aacaccaagt aagttttatg 243960aatgataaat attctgagag agaaacacac agcaagctgc agataccata cagagaagtt 244020tttgtttttt tttttgctaa tggaaccatg aagattccca ttgcatccac tcacatttag 244080tcagtgctat tttagttaat tctaatggat tcttttctct ttaagtttta tttatgtttg 244140attgacacac aatgattgtg caaatttatg gagtaaaatg tgaatgtttc cctttataac 244200tgaaaagcaa gtagcctagc aaagtctaca aatttcttca aaggcataaa agcaaaatat 244260agccaaggtg atgcagcagg tgttgcaggt tgttgcaggt gttgcaggtg ttgatgggtg 244320gtcaacattt acaggttgtg ctgattatta aactttgaat gttaatgtgg aggctgctaa 244380tgctaacact agaaagttag aaatgtattc tgaaggattt ttgacttaag taaaattaga 244440aatgagcaaa acctttctgg tcagaatgat tctacaattt tcacaagcca aaaattattc 244500aaaatgcatc tctgaggctt ttgcataagt atttaggacc tctttgaatt tcttgctatt 244560cctgtaacat atttgcttta taatacgtta tccctttgca gttctttctg gttggaatag 244620ctccctgccc ctcaggtgcc cagtaaactt aattgtcact tctgtgaagc cttacaagat 244680cagtgggaat cccttaggct gaattaataa cttcccactc ctagtggcca taccactcta 244740tgactttcct tttaaattgt ccttactcca ttatagtatt ctttgtagaa ttgtctctgt 244800tccctaacag actgtgtaag ctacttaata attccataat tccttagcta caggacccaa 244860cagtgcctta tataaatttc cataaaggca ggtttaatgg gtgaatcatt ctactttttt 244920ctttgcctct tctgtgtcac catagtacta agtctctgtt ctctttcatg gtcttttctt 244980ctccagtttg aaaaacttta tgatgtacaa taaggtctca cataacatta ttggtaggtt 245040cttgaaaact gcaactttaa gtgaaatgat gactattgaa gtcaatttta ccataggcta 245100actaatataa aaaagagata aattcttaga gtgtatttct gctcacaaaa atgtcaccaa 245160acttctaact aaaaaacaaa acccttctaa tcttaaacct tgagataaat gtaagctata 245220catccattta agaaagatta atacaaacaa gtaagataat tatttaccct attatttcag 245280ttcatggtca caggtggcca aagtccttcc tgacagctca ggtagccaag cagaaaccca 245340tcctggacag aacaatgtgc aaactccaca cagacagtag ccccgatggg gaatcacatt 245400tttttctcat caatgttata atgaaatgaa gttgaataaa atggcattga ataaaatatt 245460atttgagtgc ctgttgtaca ttactaaaaa gttctaattt gaccattaga atatatatta 245520tttttaatta gttacaaatt tatcttagaa aaaagtagga gttctagttt aagagccatt 245580tttgtcagtt tgggacaatc actttacttg tcttgacttc agtttcctca tccaaataac 245640gaggacatta ccaaatgatc tgcaaatcca gcgcccactc ccccccaccc actctaatat 245700tacggtccca tactctttct gaattcatga aaaacgcata gtctaggtca cataatagta 245760cacttaatta acagttttat tatcttgtct cattacttct ttctttgctc ttcctcaaaa 245820acacataacc aaatagtaag ttctatgatg tctgaagaac tcagaaagtc aaaccgatac 245880agatttaagt cctgattctg ccatacgaaa catctttctt gtcaacttcc tcaacatgga 245940taaggctcag tttctcatgt gtaaaatggg aataataata gtagtacttt ttaaatagag 246000tcatagctag cctccttctg catttcttac gagttcctgc aattggcagt cgtgtaagaa 246060tgcaaggtct taataacaca gatacgttca ctgatgaatt aatcactagc ctgtgttttc 246120gcccgttatg tcaaagcatt tttatctgtt tcttgctgat tgccagatac tgtaaatacc 246180taatattaca ataaaattac agtgccaact acatatagtg ccaactaaaa ttgtaaatct 246240tgcaatatat gcaaatcttc atgaagttga taaacacaga gctaaataat tgtaaggaac 246300acaggtgaag ccattgacaa attgaaaaaa agaaaactaa tgagaatatg acagactagg 246360caccataaag acactaaatg gaaaatgctg attaaataga tacatgactc taacattttt 246420cacaaaacta aaatcatgtg gcaaatcttt aaaaaggata gttttgtatt gttatgcaaa 246480tgctcaaaat taattattta attcattctt tgctctttct aaggaataat ttatggaagt 246540aatgagcaag aggatgaaat tcagcatgag aataggagca tctcacagtg ggcactgcag 246600agaaagtggg ggacagtgag aagcagcaaa ggcagcccac tcctcattct caattctgac 246660acaggccaac tctggtgctt acccttagca gcaaaatgag aaagcagtgg acaaaaacac 246720aaggcggatc attgaactct gtgtgtctga tacctaacac cttattgaag tttcaaccta 246780agcaagtcta ttcaaatatt aaaaaactaa taatgtttaa acttccataa aaccttagtg 246840aacactaatt atttctatag cattttgcaa taaataaaag taattttact gctttttgtt 246900caacgaataa ctctgtgagg tagttgaaac agatattacc tctattttac tactgaaaaa 246960ccaaatgttg gtaggagctt actcaggtac tcagagaaag aacacaggtc ctggacccca 247020gggcatattg ataaaaatgt tttatgtcat tttcatctga ccagtaatgt agttcaaatt 247080ctcattataa tctttttagg gtatattttg ttggtcccaa ttttctgaaa gataagaaat 247140ctgaggattg aagagattca ataaactggc ccagagtcag tggtaaatat cagtttccta 247200attccatgat ccctgctttc tccaggacac tctccccttt cactatgtca ggttgtttgc 247260taaaactcag aatcaaatgt ctttattaat aataggaaaa ctataccaac agaaacgagg 247320ttttcagaga ataatagaaa tttagaaagt ttgtcttaga aaaagtgact gcagttcata 247380cctcaattat aattactggt acatttagga actgaagttt aaaagtgatg catttgtttc 247440aacgttgttt cctttttctg tgtacttcct ataggtgata ctaaagagca atgaaataag 247500caaaagctat gttgtaccaa aaaaaaaaaa aaaaagaatc tgtgttttgt attaatccct 247560gagctcagta gatcattgcc tctatcatcc cattttacca attgagatgt gaactataga 247620gattaagtga actttctagg gtcacagagt tccaaatcgg ctgagcaaat atatcagtgg 247680ttcttgagac attcactgta ttttatgact atcttttctg taagcatatt gtagtaaaaa 247740tataaaattt tgtttaataa agcaacgaag agaatacata agcagaaatt attcccagta 247800aactggcctg aatttatgtg ttgtgagtga tgagtatgtt tctactacta ttgcaactgt 247860agtcatgaat tatttgcgaa caagggccaa gaatacatag tttaggttat attaaaattg 247920cagttaagaa aatattttcc tctgggaggc tgaggtgggt ggatcacaag ttcgggaaat 247980cgagaccatc ctggctaaca cagtgaaacc ccgtctctac taaaaataca aaaaattagc 248040cgggcgtggt ggcggtgcct gtagtcccag ctacccggga ggctgaggca ggagaatggc 248100gtgaagctgg gaggcggagc ttgcagggag ccgagatcat gccactgcac tccagcctgg 248160gcgacagagt gagactctgt ctctaaataa ataaataaat aaataagagt gaaatccaaa 248220cacaagaaat ccatgctaat ccacatcaaa ataaaaagaa aactaagaac aaaaaaatct 248280tgaaagcacc aagaaataaa tgacacctga actatcaggg gaaaaaaaaa tttgaatgac 248340agtggatttc tcatcagaaa tcataatgat cagaaggaaa tggtacaaca aattcaagtc 248400ctgaaagaaa agaactgtca aacccaaatt ttgtatgtgg caacattttc cttcagaaat 248460gaaaagaaaa taaatgcatt ttcagattaa tgaaaattaa gagaatttgt cacaagccac 248520atacattaaa acaatggcta aagaaacccc ttagagaagg aatcttaaaa catcaagaac 248580aacaaatata agtaactaca aaagacgttc cttctcctct tgtgtgttct acatttagtt 248640tagtagtcaa agctaaaagt acaatatttt ccaatatgaa tctaaatgta tgtagcaaaa 248700acatttaaga gaactatatt ataaatgggt tgggaagata ggtacagaga cttaaaagaa 248760ggcaagtttt ctatgtttga cataaattgg taaaatatta acaccaacaa atctttgtgg 248820tggtggaaga attctgtatc ttgcttctag cggttgttac aggaatgcat acatatgaaa 248880agatggcata gaactataca ccacattgta ccaatgtcag tttcccagtt ttgacattgt 248940actattgtta atacaagatg taaccattgg gagaaagtga gtgaagggta cccaagaact 249000atccattttc caaattatga atccacaact atttcaaaat aaaaagtaaa aaatgtaagc 249060ttttaaaaaa ttcttattaa gtcacattct gaaacacctt ctaaccttaa aaaaatggga 249120aaaagcccat aataaagagt tggtataaaa ttacagagca aatttcttca gtaattgatt 249180gttttgcttt agttaggttt ttgatcatca tttgtgacct acttgaattg gggaaatgca 249240actctgtttt gatgctgatg ttactatcaa tgtctgcttt tttttttttt ttttttgaga 249300tgaagtttca cttttgctgc ccaggctgga gtgcaatggt gcaatatcga ctcactgcaa 249360cttccacctc agcggctcaa atgattctct tgcctcagcc tcccaagtag ctgggattac 249420aggcccctgc caccacaccc agctaatttt ttgtagcttt tttatgtctt caataatcac 249480attgtataaa agctaatgag ttatcttggc taggtcataa agataactga caactttgtt 249540aacagttcct aaataaaaat attatttata atcttcagct atcagagtat agtgatagac 249600accttggaag tctacaagca tttttttctc tttcagagaa gtgaaaaaac tagcataaga 249660gtctctggat gtcttagtgg cacaactaga tagtggcatc aagctatgaa acatccaggc 249720catattacca aactttttaa tctaccgtga aaatccttgt ccttttaatc tgtagcaaac 249780atttttctgg aaactctaaa agcccatatc gctcctctca gaactgaccc cacttcccca 249840gtacaactct atttggatta tataatctcc actggtctca ctggactagt taatatgatt 249900gtctctccag ataattgaga attatgaccc atagattcaa actgggtaaa cttaaggttc 249960tatgttgcca gttggctact gctattactt ttggatttta tcaatgatga gatattccaa 250020acacacagaa aacacccatt tgctcccttc cctgaatgaa cagatattaa tactctgtca 250080taattctttc aaatttcttt ttccctaaaa attaaaaaaa ttttgatata gacacaaaat 250140taaagtagta ctagatatac caatcaccaa gataaaacaa taatcgatat tttgtcgcgt 250200tcacagaact aatttattta tttattttta aaattatggt agaaaacaca ttttaacata 250260acatgtttaa cataaaaatt agcatcttga ccatttctaa gtgtatagtt caatagtgtt 250320aacaatagtc acattgttgt gcaatagatc tctaaaagtt tttcattttg caaaactgaa 250380actctatacc catcgaaaaa caactctcta tttccccttc ctcacagccc ctgacatcca 250440caattctgct ttctatgagt tggaccaatt tagatgcctt atacaggtgg aatcatacag 250500tgttggtctc tttgcgactg ggcttatttt acttaccata atgtcttcag ggttcatcaa 250560tgttatagca catgacagaa tttccttctc tttttaagct aagtaatatt ccactgtatg 250620tatatactcc attctctgta tccatttata tgtcagtgga catttgggtt gctttcacct 250680cttggtattg agaataatgc tgtaatgaac atggatatga aaatacgtca tcaagagtct 250740gtcttcaatt cttctggata tgtacccaga gtgggatttc tggatcatat ggtaatcgca 250800tttttagttt cctgaggaaa ctctatactg tttttcttac tggctgcatt gtcaattccc 250860attgacagtg cacaagaatt gcaatttctc cacattccaa taccacttgc tattttctgt 250920ttttatgaca gtggccatgt gtattagtcc attctcacac tgctataaag aactacctga 250980gactgggtaa ttgaagaaaa gaggtttgac tcacagttcc ctaggctgta cagaagcatg 251040ttttggaggc ctcaggaaat ttacaattat ggcagaatgc aaaggggaag caagcacatt 251100ttaccatggt gcagcaggat agagaccgag agaaggggga agtgtgccac acaactttaa 251160accatcagat ctcatgagaa cacactaaca gcaaggggga aatctgtccc catgagcaat 251220catctcccac caggcccctc ctctgacatg tggagattac attttgacat gagatttggg 251280tggggaccca gagccaaacc atatcaccat cttaatgggt gatatctcat tatagttctg 251340atttgcattt ctctaatggt tagtgacatt gaatatcttg tcacttgtta gccatttata 251400actcttcgga gaaataccac tttgaggatt ttacccattt ttttaactag gctatttgct 251460tgttgttaaa ttataggcat tctttatata ttctgaatct aactccttac cagatatatg 251520atttggaaat attttctccc atctcgtagg ttaatttttc actctaagga ttgcttcctt 251580tcatgtgcaa aaggctttaa gtttgatgta ctctcatttg tctatttttg cttttattgc 251640ctgtggtttt ggtgtcatct ccaagaaaac attgccaaat ccactgttat aaaatttttc 251700tcctgtgttc ttcttatagt tttatatttt aaggtcttat gcctaggtct ttaattcatt 251760ttgagtttat ttttgtatat tgtgtaaagt aaggatccga cttcattctt ttgcatgtgg 251820atatccagtt ttctcaacac catttattga caggactacc ctttaagccc attgtatggt 251880tttggcactc ttgtcaaaga ttgattgagt gcgtgcacaa gggtttatct gggacctcta 251940ttctattcta ttgatccata tgtctgtcct tatgtcagta ccacaccgtt tagattactg 252000taacttggga tcacttaacc tatttaaaac acgttttttc tttttcttat ttgaagtatt 252060tttcagcaaa tatcagatgg tattatttta ccccttcata attcagtata actctaaaaa 252120ttatggccca ctacaaaacc acaatattat tatcactcat aatgtttctt tggcataatc 252180tcatacctgg tcttaatcaa atgtatggat tttctcaaaa atgtgttttc tggcttactt 252240gaattaaaat ctaaacaagg tccacatttt aatatgtttg ttttttaggt cactcgtaat 252300ttagaatagt tcttcctctg ctcccttttc cttcagccat tcacttatag aaattagttt 252360agttgttcta tagaatgcct cccattctgg atttctctaa ttctttatta tgtcttttaa 252420cactaaatgg aaattagcta gtggcttaac tctgtggtca tttaactata aattgaaatt 252480agcctagaac cataatgttt ttatgtctgc aagcagtttt ttaatcttat ctgcaaatgg 252540aagattctga ggacttttcc cagtttcact gggcattctc acatgtctta ccatgtttcc 252600acccaaggaa tataggatct tcttctttta gtagatgttt gtagaaaaat tttttacacc 252660ccgagtttgt ggcatacata ggttgtccct attcattttt cactattact gtcacacaac 252720ttcgttcttg ttcttgttct tgttatactg ctgtaagtga tttaccattc ctgttcataa 252780tctgaaattt atgagaattt attgcctttg atttttattt aagatattaa cagtgcattt 252840attattggct atcctaattg atttgtttgt tttccaggag gaattttggg gaaattaaaa 252900cctgcctttc tgccaggatc acatcactgg aagctccatg actctctttt tgtaaaagaa 252960aaaaaaatca cagaaacacc cacctcccaa actattctct tttacttctt cccccaagcc 253020cacccccaaa tataactgtt atccagaagc tgttatgtcc tgtttccata catgtttttg 253080tacttttact atatctacat acatcaatta aacttatgtc ctattgtttt gtgaatttat 253140atttgcgtat acattatcat atgtaaaatt tgcatttttt tattgaaaat tatgtttctt 253200gagatttatc cacattgaaa catggagctc taaatcgtta attttaaccg ctatagagta 253260ttccataatt tgaataaagc ataatttgtt tgtacaatct cccgccaagg gaaaattatt 253320tccacactca tcatgacaag gagcactgca aaaataaaaa taaaaattac attcatacat 253380gtttgcctgc acccatctgc ctgattttct ctaaaataaa tatctaggag tgaaagtgcc 253440aggtcaaaga gcatgtgcat cttcttatgc ccacatatat gagagttttc cccaagtata 253500tctacatcaa cgtctatgat gttttaattt tctacatcct tctctgtatt catttgtttt 253560catgctgcta tgaagaaata cccaaaactg ggtagtttat aaagacaaga agattaattg 253620gctcacagtt ctgcatggct gggaaggttt cataaaactt acaatcatgg tggaagggga 253680aggaaacacg tccttctgca catggtggca ggagagagaa atgccaagca aaaggaggaa 253740aagcccctta taaaaccatc agatcttgtg agaatgaact caccatcatg agaagatcat 253800gagtgtaact gcccccatga ttaaattacc tcccaccaag tcctcctatg ataaatagga 253860attatgggaa ctaaaattaa aggtgagatt tgggtgggga cacagagcca aaccatatca 253920ttgtcaatgt gaaatagtac tttattgtta ctctattata tattttattg aatgaaatgt 253980gaggttgaga acttttacaa gccctattaa tcattttgtc ttctttttct ttttctgtgt 254040atggtctgtt tataacattg ataatctctt gggttttatg tcaatttctt aatgatttgt 254100gagggtttaa aaaatattct agataatgat tcttcttgat atttattaaa tacaatgtgt 254160tctcataaaa tcatctatga attcttcctc ctatcctcct ctccattccc tttcctctgc 254220cttcgtctct atctctacat ctacctctac ctctacctct agctctattt ctagttatag 254280ctctagctct atccctgtcc ctatccctaa ccctatctct acctctagct ccacctctac 254340ctctacctct atgtctagct ctatccctac ctccacctct agtactgata ccagctctag 254400ctctacttct gtctctacct ctacttcatc tctatctcta gttctacctc tagttctacc 254460tctagctcta gctatactta tatctccacc tcatctctat ctctagctct agttctagct 254520ctacctctac atctagctct agctctatct ctacctattt ctatctctat ctcgatctct 254580atatctatct cgatctctat ctctatttcc ataacgcttt ctctccctac agttcagatt 254640ttgttcttgg aaacagcctg ggaaacaggc agtttactat actttctatt ttcccaaatc 254700tccatctcta accacacctc acccctgcta ccaccacaca gatttcttcg tggaacatcc 254760cttgtttgtg aagcattgac acttatgcaa aaaggtatta aaagtaagtg tctggcaggg 254820cacggtggct cacgcctgta attccaagca ctttgggata tggatcacaa ggtcaggaga 254880tcaagaccat gctggctaac acggtgaaac cctctctcta ctaaaaatac aaaaaattag 254940ccaggcgcgg tggtaagcac ctatagtccc agctgctcgg gaggctgagg caggagaatc 255000gcttgaaccc aggaggcaga ggttgcagtg agccgtgatc gcacaactgc actccagcct 255060ggatgacaga gcaagagtcc aactcaaaaa aaaaaaaata gtaaaggttt cctgccttta 255120tggacaacct gtttgtcaaa tatgatcaca ttccataaat tagttttaag ttcaactggg 255180gtttccctcc tttcagcatg catattatct cttcccttcc tctggacctt tcctatccat 255240gtctttacta ggattattac atgtctggtg agtgctgtaa acaaaatgtt atgtgtgcaa 255300ttaatgctaa tttggatgat aggaaacaga aatattaatt ctaattactt ttcaaacata 255360gctcatgcaa taataagaga atctgatgtc actgcaagac gatttggttg agatgataat 255420gctaatttat tgaaatgggt gggtgtcata atcagtttga aaaaaagaga aacatttcta 255480agacatacca gatcatatta atacatcacc gccacacaca aaattggtgt taacatacaa 255540attcctctgg acgtttaaga agcaatgcct ccttaaatat tgagatctag tgaatgcacc 255600ttttcaaatt gtaatatata ggtcagaggg ttttttgttt gttttgtttt cctatctttc 255660tatttggctt ttctctttac atagactaga gaaataaagg cacatgcaaa tgcatagatt 255720tatttattta gatttcaatt gaaatttttc attatgttta ttatttgtaa acacaggcat 255780ctgcttggct tcatgtgcaa agccctgaat tcattctctt cttactgaag ttcttgaaat 255840gaaagtaata ctttaatatt tgataataat agcccaagaa ggatggtaac atccaattgt 255900aagtattaca tgtcgaagaa taaatttttt acaggaaaat gcattatatt aagaatcata 255960gaagttttta ctcataataa caaatatgga agaaaaaata gcttgcgata aacataccca 256020gtccatattt gtatccaaca tatatttttt ctctaaagca gaattacaat ataaaaacaa 256080aatttagatt ccaaatggta tgtcatatta gagagaaaaa tagtgcaaat gaacagttta 256140aaactgtcca aaaatatgag taattttatt tgaaaaatga caagcattct tcagagttct 256200gggccaccca attatttcaa taagacaaat tgtcttggag aatggctcat aaactctgag 256260ccattcatta gtttattttt gtatcatcag ggagttagct tgggaaactg gcaaaatcct 256320atctggcttg aaaagtaaat gcccttggat tagggttctg tgcaacaaaa attgcaaaag 256380gctaataatt ttacttaaaa attttatgat tattaatttt cattctgtca gcctacatca 256440caattaaaag aaataaatca ttttggcggt ttgtgcctga tttttcccaa gtttctttgt 256500ttgtttgctt gttttaatat taagtatatt tctattagct ggcaacagct gttatttggg 256560tgcctcagct gactgctccc agaataccca cagcttcagt gtgtacactt tactacaaat 256620tcttgatgtc tggattacta aataattaaa accattcttg cacatgtcca atataagagg 256680gagtagataa acttcttgct tcataccttt ttccaaaagc ttttattttc aagtaaaatt 256740gattcttatt tgcttttaat ttaccagcca caaccctaat atcatttaac tagctagtta 256800ataaagcctt ataattaaag cttcgtatta aatctttgta tacacaatga tttatttggg 256860acttaaattt tgaattcttt cctccttaaa ttaataaaat tttgagtttc tgttttaatc 256920ttcaaatatt tcaattattt gttttttaaa caaacaagcc tgaactaaag gaatgattga 256980aattcataaa tgccattttt tctcttacat gatcatacta ttaaaactat aaagtttaca 257040catcaaaata agaagataac taaagacaaa ataaaaaatt gtgaaagcac caagaaataa 257100atgacacctt acctatcagg ggaaaaatct tatggactct ccataagaaa gagtagtaac 257160tcctccaaga tccctgaagg ataataatct gtgacactac agtagatact cagacataaa 257220gtagttccaa gaacaaagac tattgtctgt caagagctgc ttattttctt attttcatga 257280cagccagaaa tacattgaaa aggtatattt gtaatacatt agccacagtc taattgtgct 257340accactggag agctgctcag tgtctagttc atcacacaat aggggatgga agttgaattc 257400attctttttg aaggtttgta tggtctgact gtgtcctcac ccaaatctcc tctccaactc 257460ctacatgttg taggaggaac ccagtgggag gcaattgaat catgggggca agtctttcct 257520atgctgttct catgatagta agtctcatga gatctgatgg ttttaaaaac aagagttccc 257580tgcacaagct ctctttgcct gctgccatcc atgtaaaatg tgacttgttc ctccttacct 257640tctgccatga ttgtaaggct tccccagcca cgtggaactg taagtccagt aaacctcttt 257700attttgtaaa ttgcccagtc tcggggatgt ctttaacagc agcgtgaaag cggacaatac 257760aaaggtctaa tcagagttgg atttgtgttg cttttgtttg taacactctg ctaagactga 257820acacaaggag gtccaatttt ttccatgtca ctagcttttt cacctctttt ccacagtagc 257880ataaattcat gctaggaagt taactgtgtg ggcatgagag cttgtctata ccatgatctc 257940caaaaaactg attttaattc tttttacctt tcctaccaat atacttgcca ttgctttcct 258000cttcaaacct caactattta gtcagtggat tcagctttaa tcttgtcacc ttatatgtac 258060acagacattt ttacatgttt tacgtgaaac aagatagagc atgagtaagt aaataagtgt 258120gtgtgtatga tgcacatgtg tgtgcagaaa aatgtaatct caatttatta ttattatcta 258180gtgcaattag agagatggtc aagcacaggt aacaaaaggg tttaacagaa aactgataac 258240gtatgtcttt agggaagaaa ggattacaaa tttcacgctg gtcaaaattg aaatttcata 258300gaataaacat ctgcagttta tgctaaaatc tgcatgtagg gttaaaatat gatattatta 258360taggtagatt tcaacctttg gttgcagctg gctgaggggg ctgctggcaa cagctgttat 258420ttgggtgccc cagctgactg ctcccagaat acccatagca gttacatgct gggaggagaa 258480ggaggcaggt gctatcagat gactcctcag gaatgtgccc taaagggaac actatttagc 258540atacaggaga cttgtcatgt gactttcttt ggagggtgat tttaaagact tgtctgcaac 258600taatgatgtc tgtgatctgt acagaggtca ccttcaataa cttctcttcc tgtaaacagg 258660aaaaggaatg ctgaaaaatg ggtaagagag gaaaacaaca gggagcccca gtcacttctg 258720gacctagtag ccaatgtgac actgtttgct atccctggag ctgagtttta gtaatcctta 258780gatattttca gtttactttg tttagaaatt gaacttcaac acaatgaaca ctagtgaggt 258840tggatgttgc agccaaaaag cttaggattt gaaggaatcc tatgaatcca tcttcactac 258900ttcacagctt tgtgacactg gtttcatcat ataacttacc tacatgttat tatcccagtt 258960tgtaaaatgg tgattataat aactgcctca atacattaat ataaagatga aatgaggaaa 259020catgtaaggt gcctggcata tattagatgc tcaatattta tggttccttt ttcatcatgt 259080tgtaggctaa ttgaacccaa tctttcaacc ctcagagact gagttgggta ttagaagaca 259140ttcacatgtt tatgctagga atcaattcac tccatccaca ttggccatcc ttgttgctcc 259200ttaaacatgg taggcattct tctaccttag ggcaatggat ctggctattc cctcctctgg 259260accttctccc aagtatgcat acaaactgac tcacttcctt caactcttca cttaaatgta 259320ttcttctcat caaggtttac cctgacaacc ccacttaata ctgtaaactg tacatggccc 259380tcttcactgc tccctcagtg ccatactcct gagtcccctt accatgctcc tttgtttttt 259440tttttttaaa tacatttatt gccttctagc acttagtaaa attttaaaat tatatttact 259500gtttattctt tgtttttccc cagtagactg ttgtattagt ccattttcat gctgctgata 259560aagacatacc caaggctggg aagaaaaaga ggttcaattg gatatacagt tccacatggc 259620tggggaggcc tcagaatcat ggcaggaggt gaaaggcact tattacatgt aggtggcgag 259680aaaaagatga gaaggatgca aaagcagaaa cccctgataa aaccgtcaga tcctgtgaga 259740cctactcact gccatgagaa cagtatgagg gaaaacaccc ccaaaattca aatgatctct 259800caccgggtac ctaccacaaa acatgggaat tatgcaagta aaattcaaga tgagatttgg 259860gtggggacag agagcgaaac aatatcattc tctccctggc ccctccaaat cttatgtcct 259920caagtttcaa aaccaatcat gccttcccaa cagtccgcca aactcttaac tcatttcagc 259980attaacccaa aagtccacag tccaaagtct catctgagac aaggcaagtc ccttccgcct 260040acaagcctgt aaaatcaaaa acaagctagt tacttcctag atacaataga ggtacaggta 260100ttgggtaaat acagtcattc taaatgggag aaattggcca aaacaaaggt gttacagggc 260160ccatgcaagt ccaaaatcca gtggggcagt caaattctaa agctccaaaa tgatctcctt 260220tgactccatg tctcacatct aggtcatgct gatgcaagaa gtagattctt atagtcttgg 260280ccagctctgc ctcagtggct ttgcagggta tagaccctct tctggctttt ttcacgggca 260340gctggagttg agtgtctgtg gtttttccag gcacacagtg caagctgtca ttggatctac 260400cattctgggg tctggaggac agtggccctc ttctcacagc ctcactaggc agtgccccag 260460tagggactct gtgtgggggc tctgacacca catttccctt ctgccctgcc ctagcagaag 260520ttctccatga gggccccacc cctgcagcaa acttttgcct gggcatccag gcatttccat 260580atatcttctg aaatctaggt ggacgttccc aaacctcagt tcttgacttc tgtgcacctg 260640caggctcaac atcagatgga agctgccaag gtttgggggc tcgcaccctc tgaaaccatg 260700ggccaagcta taccctggcc ccttttagca atggctagag tgtcttggat gcagggcacc 260760aagtccctag gctgcacaca gcacagggac cctgggcttg gctcacaaaa ccatttttac 260820ctcccaggct tctggatctg tgatgggagg ggctgctgtg aagacctatg acatgccctg 260880gagatatttt ccacattgtc atagggatta acattcagct ccttgttact tatgtaaatt 260940tctgcagaca ggtttaattt ctcctcaaag aaatgggttt ttcttttcta ctgtatcatc 261000agactgcaaa ttttctgaag ttttatgctc tgtttccttt taaagtggaa agcttttaac 261060agcacccagg tcacttccta aattctttgc tgcttagaaa tttcttccaa cagatacctt 261120aaatcatctc tctctagttc aaagttccac tgatctctaa ggcaggggca aaatgctgcc 261180agtctctttg ctaaaacata acaagagtca tctttgctcc agttcccaac aagttcctca 261240tctccatctg agatcacctc agcctagacc ttatcattca tatcactatc agcatttttg 261300tcaaagtcat tcaacaagtc tctaggaagt tccaaacttt cccacttttt cctgtcttct 261360tctgagccct ccaaattgtt tcaacctctg cctgataccc agttccaaag ttgcttccac 261420atttttgggt atcttttcag caatgcccca ttctactggt accaatttac tgtattagtc 261480tgttttcatg ctgctgatga agacatatgc aagactagga agaaaaagag gttcaattgg 261540acttgtagtt ccacatgtct ggggaggcct cagaatcata gcaggatgtg aaaggcactt 261600cttacatggc agcagcaaga aaaaaattag gaggatacaa aagcagaaac ctctgataaa 261660accatcagat ctcgcgagat ttattcacta acatgagaac agtgtggggg aaacaacccc 261720catgactcaa attatctacc actgggtcct tcccacaaca catgggaatt atgggagtac 261780aattcaagat gagatttagg tggtgacaca gagccaaacc gtatcaactg tatactccat 261840aagggtttaa aatctttatt cacttcactg atgtatatca tgcacctgtt tccttgtaga 261900tagggcagaa taaatatttt ttcatgaatg aatgcactga ggcacttcag aatacatcaa 261960tctacagtgt ttaggaattg actatgacct gaattaatat gctttgttca cctcacatcc 262020ccagatcctg gagtgtagta agtacgacat gtatagtaga atgaataaaa aataatggat 262080tcaggcacaa cacagcttcc tatgaggcta gggaattacc cagactcagg caaaattcat 262140tactatcatt ctcctcttta tgaacctgac aaccaaacat aatggcttag gaattttttc 262200tgttaaatat atttgattga tattatatac ccagaaggtc aagataatgt ggaaaatatt 262260atctactgcc cttgagtcaa ggactctgat gtatatcgtg gtgaatattt atgcttacta 262320tgtcatttca agtttgaatg ttgacattta aattaaaata atgtccctca tttttgtaac 262380acaagagcaa tagaaaggtc agttgtacct taccagtgga tagtactaca ttgctcccgt 262440gtcattttga gctttgctga gcaggagact ggcttctaat ctcatggagt gatcctatgt 262500acatcaccca gttgaagaaa cacaactgat taggcctttc ctaaaaagct attctttgtt 262560cttgaaagtt tctgctctac atagtagcaa gatggcatta tctagtacaa ccttaagaaa 262620acacaccctc tcaacactca agatgaaaat gctcagagga tttgtataga gtcaaatcca 262680aagtgaaaaa ttactttata gttgtgttat tgggaggagt ggtggcaata agacataaaa 262740taaggagggg taaatgttat gaaaacaaga tagaaagttt atggtaatca tagtaaattt 262800ctgtcaagag agctgcaatt cagatatgct ccattctcac ttgaccttta tttacctcta 262860ctcccagtca ttaaaatgat ggtctttctc ctgagatagt tctataatgg atactttggg 262920acatattaat ctgatttcac acaattgctg tgctcagtca tctgtactca atgtgtcata 262980tatgcctgaa caaccagaca tcatacaaag gctttatgtg atgctagtat gtgatcttgc 263040cttataacaa cgcatttatc acttaaatgt gcctttgtct tttctcctgg aattaagaca 263100gctaaagatt tcacaagttg cacctaagaa tgatacccca ggaaagcact ggacctgttt 263160atattccacc tcatcaagaa cacagtcatt cacttatttg atcacacagg gtaaaggaga 263220tatgcaacac ttttctgaaa ccatgtgaag agaggagaat aaatcaagca ttctggtaaa 263280caattcatgc catcaaaaac atgttttaat gtgtgcaact gtctttaagt gctccaaacc 263340cagacatctt actttacagt cagagctccc aagtggcaaa caaaagcacc aacttgctca 263400tgattcattc taatcttttt acataaaact ggaactagga accatagtca aggaataaga 263460aagctgatcc agaatctacc actataaatt attcagctaa aaaaattaca taaatgcatg 263520cagaagtcag gaagaagaaa tcaaaacata agatcataat cccagtagta tgctgttaaa 263580acatgaatga acaaagattg ttggttctat gtagagatac tgtattgaat ggaagacgtg 263640aaaagctgtc ggctcccaag actctgatga cataagtatc aaagaatgag aaatggactg 263700gatgattagt gctaaaagga acttttaagg acatctaatg caaacccagt aattttacag 263760aggctttaaa tagatttgga gaagttgaat aatgtgctct caatccataa ttagttgata 263820ctggtgaata taccagaaaa acagaacaaa ccatctttgc cattaaagtg tatctgtctt 263880attaggttta actaatatag tcagacatca ggtcatgtga taccaaatgc ttgaactggt 263940caggtgttgc actttttgtt cccagaattg atagatggtt gttattccat tctgccattg 264000cctttgtttg ttatcatagt aattataatg ccctatttac ttcagctaga ggattaatgt 264060ttccggatat cttttcttct gcctagacat agattatttg tataaatact ctataaaatg 264120gagttcccag gttttgttcc tattcagtta ttctactttc tatcttgcaa ttaactacaa 264180agaggtgaat acatagcact gagaatgagg caaaccaagg gcctgccatc aggtgatttt 264240aaacattgaa cattggcaca gcaattaaac tcaaccaact gtagagttaa taaaaataca 264300gatccagatt gctagtcaac tatttcagat gcatggttct ggttacacag tgaaaactta 264360ggagccttaa ggacactata gaggttgctg cagccagttt agtcaacgcc ttacaaacca 264420ttacagctga aagtgctgag aatgatctag tagacaattc tcactcaagg tcagaaagat 264480aactgatggc tggaccagaa tggataccaa gaatttctca cacgagacca ggaatctctc 264540caaacaactc ttaccttgag gataatgtaa cttcgaaaga aatttgtaca tatttttctt 264600agttctgtgg aatatttaaa tggtcccaca taatttcaaa taaattctgg ttgaagtttc 264660gtggatcttg gtttttttca atctacaagt tatttacaag ttttaaaact actgagttag 264720gttttctttt ctttgtttat ttaagaaaag ggaggacaaa tagcccgaaa cttcaagctg 264780ttcatgctga ggaattggac attatatatt aaggacacaa gaggtatgag aattcctcag 264840ctacagtcgg cagcaaatgt gttcgacaag ctgaaaggtt tagcactaag tacaaggcaa 264900gagagataga agataaggaa ggaaagctaa ggaaataaat ttttcttaca aaactcctct 264960aaagtattct ttttataaca ggtcttgcca gaacttagat aagccagaat gactacgtta 265020ttcttccaga aaactgttta tgaatcagtc tgctattttc tacccaaaaa aaaaaaaaaa 265080aaaaaaaaat cacagtactt cagcactgag gggattagtg gctgagcaat ctccttcaga 265140gtgctgctca cttaatttgt taaaggacaa agtagttgaa tcatcttact tgctgaatct 265200acaggaaaaa tactgtcact tctttattgt tgtcattttc cagtgtttat tatgtcataa 265260aatgttaggc tttgaagtcc caatctgact gtttagagca gggatctcct gccaactgca 265320ttatgtcatc aaatgagcaa acataataaa cataattaac atagcaagca tttttcaagt 265380cactggaatt taaaagttgt ttgttatagc tactggtgtt acttaccctg acttatgcac 265440aaaggaaaat agtttcattt caaataggta gttttttccc aaatagctat acatattttt 265500taccaatatt ttattctcct cttctgtata cctagaggat gtgattattg tcttccatga 265560aactgaataa agtattgtga ctagatctag ccaataaatt gtaaatgaaa attattaggt 265620tggtgcaaaa gtaattgtgg tttttttcca ttacttttaa tatgtgtggc tgtgattgtg 265680gatatcgtgt gttcaattat ccaggctttc ttttggtgat cgagacagtt tcatgttcta 265740aatgttgcag ctaccacata gtaaagcccc tgttggcctg ggtttctgag tgactctaga 265800gttgagtccc atgtcaacca aaaatagtta cacaatttga gtagaaaata aggttatatt 265860gtgttaagct gctgagattt tcagttggtt atcatagtac aacttaccct acacaatata 265920tatgactcat gggataaaaa atatatatgt atatatttgc atatgtgtgt atatttgtat 265980ataagcatct atgtgtatgt gtatatatgt acatatatgt atatgcatgt gtatatgcat 266040acatagtaca tatatacaca cataagttta tctatgtgta tgtacataga cacataaata 266100catataaaag tttattagtc agaatatgca tgtatataca tgtgcataca tgcacatgta 266160tatataatat ggttactaaa ttgttgcata aagaattctg ataatatatt agtgctttat 266220tttacagaaa cgtttcttaa ttatgtcttt aaaagaaaac agttttcaac aatttaggtt 266280accttttata acattatacc aaaacgcaag caaaccatag cctagatggc aatctctgct 266340aaaggttctg caggtgctaa ttatacccct tgatcttttt acattttaac tagtatgaaa 266400ataaatacga aaaatatgtg gaattgtgag tctcaaaata ttagcttttc accctaaacc 266460tctgtactac ctaatatgaa ctgactcatc cttctactta cagggagagc cgctggagga 266520ctgtgggctt ccctttgcag agctaaaaat gctcttctct agtgagttta ctgcacagag 266580gagtaagcag aggaaagcag gcactgcttt aggacatgtt ggagagggag caagagtctg 266640ctgtcttgca ccacaatgat actcacctca tctcatcctt aggagaagta gaaaacaggg 266700caattgggaa gacagactcc tggggttctt ccaacattgt ttcctcttgg aaagactcag 266760aaattctttt ttagctatga aattttaaga agttgattaa tatgctttct cttccacccg 266820caacaacctt gtatgagtct tccacatctt tgccattatt accattcaac attaaatgct 266880gtgtcctctg actgaagaga catgatgact aggtgtatgt ggtgttctca attggatact 266940gaaacagaaa aagaacacga gtagaaaaac agacaaaatt ttaacaaagt ccgaagctta 267000gttgatagga atgtactgat gttaatttct tagttttgat aaatatacca tgcttatata 267060aaatgttaac atttggggac actggatgaa gggtatgtaa aaactctgta ctacttttgc 267120aactcttctg taaatctaaa attatttcaa aacaaaagtt taaaagaaaa atattttgtt 267180ttacaggatg gggagcacaa tcattagaaa tttatcttgt gagtagaaaa ttcatggcaa 267240agcctgccta aaggcaaagt gaattaggtg ggatgaacct aaagagatat tttggggctc 267300ctcttttaat attctaattt aagcataata ataatacgct tcttgtaatg ctctgtgact 267360ttgaatagaa tgggaggcag gtttgcccta agcaatatcc agctcgagtt ttccttagtg 267420attttggagg tccaagatat tttcctttca caggtcaatg aataagaaaa ggagcaaata 267480cattaaaaag ctgacctacc gtatgcggct gacttctcat aattttgtga atcctaaaag 267540gtattctttc ttactttgtt ctcagcttcc tttcatctga gaaagttctt aaatacctag 267600tatccaagaa tcttatctac ccttctcaac tcctagatga aggggggatc ttaaaagttt 267660cttcctcacc attccttgaa gggggttcta tagacatatg gagttaccct ggctggatga 267720agccaccctg caattaacct cagtgttcat gtaatagata tgagtttgtc acttaatttc 267780aacagcaaac aacctcgtaa aagtcaacac agaagggaaa aaaatggtta ctagctttgg 267840aattagaaac aactagattt aaatagcaga tgtgtcactt tctagccagc ttctattaag 267900ccaaaaaatt tatcagattc tgagtcagtt tgtttgtctg taagactgta atagcctacc 267960tacaaggttg ttgtgagatt taaatgacac agcatatgta aaatagttcc tgggattaag 268020tagtgtatct caatatattg gttctacatt ctctgaaaaa agcagcgttt gattgtagga 268080tagcttgaaa gtgtgaccgt caagttggag ggaggggccg ggcgcggtgg ctcacgcctg 268140taatcccagc actttgggag gccgaggagg gcggatcacg aggtcaggag atcgagatca 268200tcctggctaa cacggtgaaa ccccgtctct accaaaaata caaaaaatta gccgggcgcg 268260gtggtgggcg cctgtagacc cagctactcg ggaggctgag gcaggagaat ggcacgaacc 268320cgggaggcgg gagcttgccg tgagccgaga tcaggccact acaccccagc ctgggcgaca 268380gagcaagact ctgtctcaaa aaaaaaaaaa aaaaaaattt ggagggagga agaaaatgtc 268440agtgcaagaa gtctaggcat acaactgcgt ggtggtttta ccgaaagata tgtacagtac 268500ttcacaatta tataaatgtt ggattatggt cacaggtata gcttaagcac ctttcaatct 268560attttcaccc tcatgccact gctcatggca tcatagaaag gtgtacaaga tgcatgccct 268620ggcagtatca agagtcattg aaagacacaa ctcagctgcc tctaaatggc aacttctttt 268680tatgttaatt gtataataaa gactatttag tcaaaatgag tttatcagtt gttactttaa 268740gactttcagt actcccattt ggaacagaac aaattttaaa ataagattga tacaaaatgg 268800tgttcatttg catgaataga aggtttttgc ccaaccagat caaagcacat tagtggattt 268860tgagctcaaa tttcaagcaa tttgcaactg tatacctgac aattcacaga ttagaaataa 268920ttttatgaac attgatcata gtggaaatag ctgtaataca aacaagagct cttagcaaaa 268980ggtttcaagg ccttataaaa ccaactaatc atgtaatgat tttttgttct gtttaattac 269040aagtcataat gcaatgtaat taaacccagt ggcaggagga taacagtacc aagatgcaga 269100caattaagtt aactacaaca tatgtaagat atatttatgt acccgataaa tcttctgaca 269160ggaaggatct agatctacct taattaaaat aagcattttt cttgcatttg cacataataa 269220aaagatacac agtttccata ttccaagcag taggataaac agtcaatgac tgatgtttaa 269280gaaacatgtt taaaaacctg aatttaagag tctaagaatg atttcttcta attcaggtgt 269340ttagagaaac cctgggtgaa ggggaaaaaa gtaatattgt ttggtaaaca taagtgtctc 269400ttttaaaaaa ttgatttgca gtttattaca cattctgggt aataattatt tgttatatat 269460atattgcaaa tatctactcc cagacctgta actgtttttt tacctttatc ttatcttttg 269520aaatacagat ttttacattt taattttgac atatttatca atctttctta catggcctgt 269580gcttttgtgc cttatttaaa aatacctttc caaccatata gtagcatata ttctcccaca 269640ttttctacta ataatttttt tgtagttaag ttttttattt ctctgatgtt aatttttgtg 269700ttttgggtga aattagggcc aatttcactg ttttaatgga aatccattaa ttccaacacc 269760attttcttac tttcttattt tgttcaactg agacaagcta gttatttttt tttcaccagc 269820accatttttt tttactgttt tataacatca tttctgtcaa atctttatat atttatgtac 269880gtattatggg attctctact ccattacata tctctattcc tatactaata tataactatt 269940ttaactttta taacattgta ataaatcttg gtatctagca gcccagccct cactcaaatg 270000agtcttgact tttcttactc attcagtcgt ccatattaat tttagaattc tcttctcatg 270060ttccatgaaa aataattgcc ttttttatga tattgagtct tccatataca tggcataaat 270120ctccatttat tcatattttt atatgacctt taataatatt ttttaattgc ccccacaaaa 270180ttcatgcaat tgttatattg atcaattcca atgaaattca taattttgga cactttttag 270240taggatattt tttctcgtat gttttctaat tggtcgaaga gcaggagtag gcatccttgt 270300ttttgacttc aatgagaatt tttctaaata gtaacaaata ttaaaaaatg tttaaccagt 270360agactgcaag ctccatgaca acaaggtctt gattcttttt attgctatat cacaatgctt 270420aagacaagta tctgataaat gtggatataa aataaatatt tataatctga ataaataaag 270480atatgatgca ggtttttttg gtagaaatct tttaactttg taatcaagtt ttcttctttt 270540gccagttgtg ttttaaaaat catgaactac tgttgaattt tgtaatttat tctttatata 270600ctgatataat tgtatgagtt tttgtccaat aattgctatt gttttgaact ggtgacttcc 270660ctaatatata actatcttta ctagtgataa ggtattacat ttttgtatat tgtagaattt 270720gttttgctga tattttaata tatttccatc tctttcaaaa gtgatattgc tctatgcttt 270780tgttgagtct gattatgtgt cttttctaga caatatgtag taagaccatg ttttattaat 270840tcattttgcc aacttctgct tttgactaga gtgtttaatt cctttacatt taatataatt 270900actgataagg taggatttat atctgccatt ttactttttt tctatatatc ttataccttt 270960cttgttcttc taatcttctg ttctgccctt cttttgtgtt aaatagatat tttccacttt 271020acctgtatta gtccgttttc acactgctga taaagacata ctcaagactg ggcaatttac 271080aaaagaaaga ggtttaattg ggctttcagt tatacgtggc tggggaaggt tcacaatcat 271140ggcataaggc aaggaggagc aagtcacatc ttacatggat ggcagcaagc aaagagaggt 271200gtgcaggaaa actccccctt ttaataacca tcagatctca tgagacttat tcactatcac 271260aagaacagca tgagaaagac ctgcccccat gattcaatta cctcccacca ggtacctcct 271320acaacatatg ggaattaaag atgagatttg ggtggggaca cagagccaaa ccatatcatt 271380tggccctggc ccctctcaaa tctcatgtcc tctcaaaacc aatcatgcct tcccaacagt 271440cccccaaagt cttaactcat ttcagaatta actcaaaagt ccacagtcca aagtctcatc 271500tgagacaagg caagtccctt ccatctatga gcctgtaaaa tcaaaagcaa gttagttact 271560tcctagatac aatgcgggta caggcattgg ataaatacag ctattgcaaa tgggagatat 271620tggccaaaaa aagggggcta caggtaccat gcaagtccaa aatccagcag ggcagtcaaa 271680tcttaaagct cctaaatgat ttcctttgac tccatgcctt acatccagat cacactgatg 271740caagaggtgg gttcccatgg tcttgggcag ctctgccctg tggctttgct gggtatacct 271800ccactcctgg ctgctttcat gggctgacat tgagtgtctg tggcttttct aggtgcatgg 271860tgcaagccgt cagtggacct accattctag ggtctggagg atggtggtcc tcttctcaca 271920gctccattag gcagtgcccc agtagggact ctgtgtgggg gctccaaccc cacattaccc 271980ttttgcactg tcctagcaga gatccatgag ggccgtgccc cagcagcaaa ctttgtctgg 272040gcatcaaggc atttccatac atctgatatc taggttccca aacctcaatt cttgacttct 272100gtgcacctgc aggctcaatg tcacatgtaa gcatccaaaa ctaagggtgt ctaccctctg 272160aagcaatagt ccaagctgta ccttgccccc ttttaatcat ggctggagtg gctgggaccc 272220agggcaccaa gttcctagac tgcacacagc acagggacct tgggcgcacg aaatcatttt 272280ctcttaggtc accacactca tgcctgtgct ttgaaggggc tgctgtgaaa acctctgaca 272340tgccctggat acattttccc attgtcttgg ggattaacat ttggctcctt attacttatg 272400caaatttctg caagcagctt gaatttctcc tcagaaaatg gatttcccca ttgtcttggt 272460gattaacatt tggctcctta ttacttatgc aaatttctgc aagcagcttg aatttctcct 272520cagaaaatgc attttccttt ctattacatt gtcaggctgc aaattttctg aacttttatg 272580ctctgcttcc cttataaaac tgaatgcctt taacagtacc caagtcacct ttagaatgct 272640ttgctgctta gaaatttctt ccatcagata ccctaaacca tctctcaagt tcaaagttcc 272700ccaaatctct aaggcagggg caaaatgctg ccagtctctt tgctaaaaca taacaagggt 272760cacctttgct ccagttccca acaagtcact catcttcatc tgagaccact tcagcctgga 272820ctttattgtc catatggcta tcaggctttt agtcaaagcc attcaataag tttctaagga 272880gttccaaact ttcccatatt ttcctgcgtt tttctgaacc ctccaaactg ttccaacttc 272940tgcctgttac ccagttccaa agttgcttcc acattttcag gtgtcttttc accagcttcc 273000cacactactg gtagcaattt tctgtattag tctgttttta tgctgctgat aaagacatac 273060ccaagactgg gcaacttaca aaagaaagag gtttaattgg atttatgttc cacatggctg 273120gggaagcctc agaatcatgg tggaaggcaa ggaggaggaa gttatgtctt acgtggatgg 273180cagcaagcaa agagaacttg tgcaggaaaa ctccctctta taataaccat cagatctctt 273240gagacttact cacttttatg agaacagcat gggaaagacc tgcctccatg attcaactac 273300ctcccaccaa gtccctcaca caacatgtgg gaattcaaga tgagatttgg ttgaggacac 273360agccaaacca tatcagtact attttaattc tatcattgtt tctttaatta ttatatattt 273420ttgtgttatt ttctcagtgg ttgcctgggg actgcaatta gcatcttaat ttaaaacaac 273480atcatttgga ttagtaccaa cttaattgca atagtatatg aaaactttgg tccaatatgc 273540atccatttgt ttttgttctc tgtgttattg ttatgcaaat tatatcttta tatataatat 273600gcctttaaac atgttttata attattgtgt tatacagttg tctttttcac taccatattg 273660tttcctttat gagtactatt tctttcctca tgtggattca agttaatgtc tactatcatt 273720ttatttcagc ctaaaggact tcccttagta tttcttgtag gacaagtctg ctaacaacaa 273780catatcttag ttttcatttc tctgataatg ttttaattta ttgaaggata tttgaatata 273840gaattcttgg gtgatagtct ttttccttta gcagtttaaa tatatcatcc cactgtcctc 273900tgatcattat ggtttctgat ggaaatcagt tttaatctta ttgaggatcc cttgtatgtg 273960atatttccct ctgctgtttt caggattctt tgtctttcaa tagtttgatt ataacatgtc 274020tagaaacaga tctaattaag gttctcacct ttgaattttt aagcttcctg gatcaatgtt 274080tttcacccaa ttggatgaag atttcagcca ttaatttttc aaatattctt tctacccctt 274140tctctctcct tcttcttcag aaactccctt tgaatgtatg ttggtaagct tgatggtgtg 274200cacaggtctt tgttaattat tcttgattat cttttccctg taccccagac tgggccatct 274260caattgatct atcttcaatt ttacggtttc cttttttttg gaggggaggg tgctatctta 274320aatctgtttt tgagcttctc tactgagtat tttcttttaa ttactgtagt ttaaaacttt 274380atagatatat atataatatg tagtattgta tatataattt ctgtctcttt attgatattc 274440tctatttagt gagatattct tatatcatac ttttcttttt taaaaataca tggcttcttt 274500tagttctttg agcatattta caatagatta tttgaagtct ttttctagta agtccaaatc 274560tggacttatt caagaacagt ttctactgac attttttttt ctatatatga gccatacttt 274620ctttgttgca tgtctctttt tttgggtgtc aactcaacat tttaaatatt attgagtaac 274680ttttgaaatt atattaacct tctttccagt gtttgttgtt ttggttaaat gctattttgg 274740ttatgattgc tgtggttgct gttttttctc tttgtttatt tagtaacttt tcttttctga 274800ttatgtaaat tctgagtttt ttggctgatt ataccactgg acttcagtaa attcttcagg 274860acagagacta tgttctgtac caacaatgcc tcagatagtt tctggccatg ttagaagctc 274920aatacatttt ttatgtaaaa gatgaacaaa tagtatcaat aacacttttc tctgatgtgg 274980ataatgagga gatggaaata atagtataca atttgggtat tgccatttat gtggtactta 275040ctactaattg acacatacac tataatgatt ttaaatacaa acactgagtt atgatttagt 275100aatgttttaa ttttgaattt tcttattctg ctttaataaa atgttggact atcatcattt 275160aatatttgaa agctgaagtt tttatgcagc tttcggtcac tagcagttaa aatgcccctt 275220gcaatttttc tctatttgct tttaaaatta aaccaattta aatcgtatct atggaatacc 275280tgttactccc aaaagggctt gaaagtctca acattttctg ttattcgcac ttggagaaat 275340gaatttctgt tgcatgtagc tagcatcgac tgccacaaga gaggtaatgg ccattggata 275400tacattcaca tctctgacag accttctagg acaaggagtt accaattaga ggtttctcag 275460gaagctttta ccccaacagc taattgataa aagagaccgt ggtttcccta gtaatagctg 275520tagacttttg aaattgaacc agacttacac ctctgcagca gatataccag cttattaaat 275580aggtgatata gtttgaagaa tcaaaaactt ttttaaaaat ctgaatattt acctttttca 275640agtgaaaata ttgaaagttt ttaacagcct ctgagcaaac aaaagttatc ataaaatcta 275700gcatgtaatg tgtccaatat attgaagtgt gtcatttatt ctggactaag tcaaggtaca 275760cataaatgta tacatatacc atgagaaagg cagataactg tggtacagca gaaacaattg 275820gatataactc atgaatgaga agtcagagtt atcagcctgg aggggagcta gaataactat 275880gaatgcttag ctgtaaataa cacaaagaag acaatcattt gaaggagttt caaatttgcg 275940aagctgatgt gaagtggaga acaaattatt ttttcattaa tccagtctga taccttgtgt 276000gatattgaca agtgcttctg cattctatgt taatttaata aagtcatctt gaagaatctg 276060cactatttac aacactcaag attcccctta atccttagat cctcataaat gtggcttttg 276120catcctctgc ttatttacga ttagatgtaa cattacaaat ttaaaattta tctttctccc 276180tcagaaagaa atagaagtca cagatatcag cattccctac acttgccagc cacatgggag 276240ctctggagta aagaagggac aacacaactc agaaagcaat tgctttgctg aaagagctga 276300ctataggtta aggcacagct cagcaatgag aaaaagaata aaaattatgt ttttgtattc 276360actgttgtct agtcctgaaa attaaaaccc atttccattc ttattttcta aatagtattt 276420caaagttttg attctcataa aaaggaaata ttataaatct ttaattgtat taatgaataa 276480tgaatgatta tattccaatt aacactaaat catcaagttt ttcatgtcat tattagtgcc 276540aacatacttg agataattac aagtttacct tcagacaatg tggtttttat tgcatcacat 276600aaataatctg gtctcatccc caccaactcc ctacaacaaa ataaattcct aaaaagctga 276660aattcccact tatcaaagtc tctctttatg aagcaaacat tgatgtacct agaagtttct 276720tgttcaaata tcaatagact gaaagccgag tatatcaaga cttagaaatc tttaacactt 276780tagtgtggat agctcatcag tgtctttttt ttcttccaag aaactgggaa gagaaaattc 276840tgaggttcat aagtgtcctg agcagttaga gtttcaccaa ataactcaca atcaactgtt 276900aactagtagc agttcagagg gagtgagaca gactggaaag acattcttga tgaagtagaa 276960gagggagaga taggtccata gcttctcaca cacactaacc tctaggtttt tacaccttct 277020cctctactga aactctctac tcctctgtcc caaccccacc tctctacctg gctaattccc 277080tcacctttgg gtcttagtat aattatcatt tcctcaggca agggttctct ggctcctaag 277140attaggatgc tctcaaaggg tacttaaaat ctctggtttt gcctatatca aacaccaatt 277200aattctctat ctaattatgt tttaatatca tctttccaac tgcactgtaa gaaacaccat 277260gacagagcca cagctgtttt actcatgatt ctttcccagc ccctgaaaca aagtaataac 277320ctaaaaatat ctatggactg actgttgggt gcaatgcaaa tgcatgcagt aagcttggca 277380gcaagttctg catggcaaga cagggataga aatctgggtg gttgtgtaag ccttggaagg 277440tcatgattag gaatggaagg gaaagagaag agaaaaggag agaatatttt caacttcaca 277500atttgcctaa actttgttca tgctatttct tattggcccc cactttttct atttcttgcg 277560gcctcctctt tgtgtgtttt ggtaggaaca atgctaaaat tatataaaaa cttatgtagt 277620gctcgccagg tgccaagcac tgttctaaat gctgcacata ttaactcatt tggtcataat 277680agtagccctt atagaaaaat aactttgtta tctccatttg cagttgagga aactgaagcg 277740ttgagagggt aagtaatttc ccaaattcac acagatagaa tattgtagac ctagggttgg 277800aactcagcac tctgacttta gaatgcatgc tcctttaaga ccatcctggc taacaccgtg 277860aaaccccgtc tctactaaaa atgcaaaata ttagccaggc atggtggagg gcgcctgtag 277920tcctagctac ttgggaggct gaggcaggag aatggcttga acccgggacg cggagcttgc 277980agtgagccga gatcgcgcca ctgcactcca gcctgggcga cagagcgaga ctccgtctca 278040aaaaaaaaaa aaaaagaatg catgctctta aatgctttgc taaggttatg tggaaagata 278100gtgtcgatga catccctgct taattaacat ctgtttccaa acttcttttc ccttgcccat 278160atcctttata ggaaaagtta aatttgattc tttcccagtc tcttgcatct agtggtggct 278220gttcatgcca gttctgatac atatggcaca agtagatgtc ttttgaagag agaatacttc 278280tgggaaagca attgcttttc tgaaaacata ataggataaa tgtgggtggt gccatatttt 278340cctttgtttc ctgtctggaa tacagatatg aagcctggag ttctagtagc cattttgtat 278400ccatgaagga aaaccaaaga atcatagcag tcatagccga agccttgata atatagaggc 278460aatgaaccaa tgtcagcacc taacggtata acttgtcctt attacgtcat taaaaataaa 278520ctacagtttg cttattcact cttggtaatg ttttctgtta gttgtaattg aagacaagct 278580gaaattatac aagcaacaaa aaacattatc aagagtgata aacaaattga gatttctttt 278640ttaaattaac ttcctaacca aaagaatgga gacctaaggg acttctgaag caataccaaa 278700ttataatgat aatttgtaaa ggaaaatatt gtgaagggct agaattatac cacactatac 278760tagtcagaat ctcagcagag aatagatcaa acattcagaa ggcttaacgg aagacagtgt 278820aaataagatc atttatagag ggattgacag ggtcaaggaa actaacaatg gatggtgaaa 278880caccaagaat caagcaatga gcaggaagct gggcctgata aagctaacaa gggaaaagag 278940ttaccagagg ccagaaacac ctggatttgt gaaagcggct ttaccacaag ggagttttgt 279000atgtagaaat acttagtctc cggcagacca cagtagtcag ggctggagaa gggggtaggt 279060acgtaactac accaatactt atcccttctg actcctgcag gaggcctccc atgagtaagc 279120ccaattagaa gccagaggac aggagactct aggagatcta agtggtagag attggcctgc 279180tgggcactga gagggcagag aaggactgag aatagatctg gaggtgggaa agtgggtgga 279240agggatacaa atggagaata taatcaagct tgctctggca cagacagcaa atagacttcc 279300ttcttctctt tgtcttcttt tcttttacag atatcccaga aattagagta gaatatttga 279360taaaaataat aaaggtaatc agttctacct ccccccaccc acaaactgtg tgacctgggg 279420caagttacta gccactgtct caatgcatcc caagcatgct ccatatcctg gtggcttcag 279480tgtcctaaaa aatggctcat ctgatactct agttatttgc tttcttgatt gactcaatga 279540ccttcattca ctcccactca tgactacact ttggaccttg ttttcactgg gaaccgctcc 279600actattgaaa gtctaacaaa aataacccac aatcggcata caatgttgtg gtaggttgaa 279660taaagaaccc ccgtccccca aaatgttcac atcctaaacc ctggaaccta tattacctta 279720tacagcaaaa aaaaaaaaaa aaaaaaaaag ttgaagatat aattaagtta aggatgttga 279780gatagggaag tcatcgtgga ttatccaggt gggaccagta acataatgac aggcatcctt 279840ataagatagc tactactgca gaagaggaaa cagcaatgtg atgatggaag cacattggag 279900cgatgtagac accagaagtt agaagataaa agacagattc tccctggatc ccccagaagg 279960aactagccat ggagacacct tgattttagc cccgtaaact cttcaaactt ctagtctccc 280020aaactgtaag agaataaatt tctgttgttt taacccacta agtttgtagt aatttgctaa 280080agcggcgcta ggaacatgac acaaatgtcc tctcttttaa agctccccat actttcagta 280140actcagaaat gctcactggt gctttcactt tcccctagcc atgtgctgtt gtccttattc 280200catctctatc tgcaggctca cctgaacacg cctcccatca acatcatcat ttcccaagca 280260tcacccccct ctcctcacac ccactcagca gacaccaact ctggggtcag tccttgtacc 280320cgtcttctcc attcctacac ctggaattcc aagaacttct gaagaaaatg tcaccatctc 280380aggaaaaatc tatgttctcc acctagtttg ggatagcttt tttctctaat tttcgtaaac 280440atctatgtca gttctttacc actcttcttt atcccctcct ccaatctcct tctattatat 280500ctcagcaggt aacctcacct atttcagaaa actgaagaaa actgaaacca gtcatgaaaa 280560acaaggatat tttctttggt attttctttc taaataccta tttacattct tttccacctt 280620tacctctttt tgttcaaatt tctgtttcct gcctacgtgc ttagaagtta tatgaaaaga 280680tatacgcata aatgcagaga taaatataaa caaaagcaaa atgacagcag cagactatcc 280740ccaccaacag cagtagagaa ggcaaaagtc tggcacattt cttaaaaact gcactaactg 280800tagtaggctc tttggagtct aagattctat tgcaacctat tgggagaagc aaatatttaa 280860atgaatacat tataatacaa tgtgcaagtg taataggtga tgtgggtatg gatatgggag 280920aagcagagag gagggagtga gcaactctgc tagaatagtt aggaaaagaa gcccaacatt 280980ggccagaggc ttcatgggga ctactatgat ggaacaggac cttaaaggtg gagtgccaaa 281040gaaagtattc accaaagagg gaccagtaca tccaagagct ggaggcattg aaatgcatag 281100tgtttccagg gaagagtcag aggactggaa ttgctggaac attccactgg tgttaggaac 281160tggaaggaaa ggttctggag acatagtcgg ggccaggttc tgcaggctta ggctgagttt 281220caatattaat ttgaggctaa ttttcccaag ttgacctgat caaaagaatc acataaaaca 281280tttgttaaaa atatacattt tcaggacctt tctctaaaga tcctgactta agtataggat 281340taagtattgc ccccaggaat ctgtattgta atgaaatgta ggaacggaaa gaaggaaaaa 281400ttttagaaat aaattttaat tttaattttg ccacctatta aatataagat ccttagaaaa 281460acacataacc tccttgagtt tcacctttga aaagtggaaa atgacctcta ctttgcagaa 281520ctactatggg aagaagaagg gatgtaataa agtgcctggc atacagtaga gaggccaaag 281580taatagctat tactgttact tttgccatta ttatgtcccc tattgttcat gagttcaggt 281640cctctctttt tctggactat tatagtaact ttccaacaag tcttcttacc tccagtgtca 281700cctgaacaag cagcaagaga ctgtgcaggc acaggaaatt aaaggctggg atgaagcata 281760agaggtaggg gccaaaagaa agactgggca aaataagtca gacacagaaa gacaaaaact 281820gtatgatctc acttgtatgt ggaatttaaa actcagactt atagaagcag agtggttgac 281880aaggatttga ggggaagaga aaatgagttt ttggtcaaag gctacaaatt tgcagttata 281940aatacagtaa gttctgagga tctaatatat aacatggtga ccatagttaa taatactcta 282000ttgtttactt gaaatttgtt aagaaggcag attttaagtg tcttcaccct ccctccgaaa 282060aaaaacacaa acaatggtaa ctatggttag tgatagatgt gttaatttga ctatggtaat 282120taacactcac acagggttta catatatcaa atcgtcatgt tgcacacttt gaatatgtac 282180aatttttatt tgtcaattaa ttatttcaag aaagaaacaa acaaaagact aagctaagcc 282240atagctctca tatcaggcaa ccgaactctg aagaattcac atgtgcttct aagaacagtg 282300tcagaggaag agtgaaaacc aacagacagt atttcagatg tggagtttta aattgtacag 282360tactatggtt tacaaacaac caagaggtac agaaccttcc caaggtggtc ttcaggggaa 282420gaaaaggaga tttaatttag caccactgaa ggcagagatt ctgaaaaaaa cctaaaacca 282480ttttaggtgt atgccccttc aatttcagac tgttcaagaa tttggtaaga gggctgatgc 282540tatcaaagat gacatgcagg tctctggcct gggaaaatag gtgaatagca gtgctgtgac 282600atgaggtgga aatatagaag cccaggtctg ggcatagaga tttgtgagtt gaatggctga 282660aaatgttagt gaaagcatca ctggggatgt gatgacatgg gcagagtaga attaagggga 282720tccaagaaga aaatcgtagg aagtcttaca tttaagaggc agtcaaaaga aaagtagtca 282780atgaaggcac cagagaagag agagaataat acacaaaggg gagactatgg ctcgtgaacc 282840caatttcact tccacaattg tgccaagggc cagactagtt gagcaagctc tgcttttctc 282900attcccgcca ggaagacaaa gggttttgtg tttcaagaaa gttctgtgat gtgtactaaa 282960ggtaggcaat atacacttgt cattttcttt ggcttagaac tctagatagg ttcactggtc 283020tgtaaaacaa atgcccaaga aagaaaacac ataatgtatt gtttcctaca tgatttgggt 283080acatcaagca ccaattataa tacattgcct cttcatacac tgcactgacc ctagagtcat 283140aattctataa gccaggaaaa agggtagcta gaattaaaaa aatgcatttg tcatgagttc 283200taaaatgttt ttctcaattt gagaacgcca gattgtgaac tggtagacag atttagtgct 283260gtcttctaga tcacatgtcc ctctgtccaa ctcataggag aatgtaaata aaatatattc 283320aagatatatt tattgtgaac ataggtcagt tagagctaca gannnnnnnn nnnnnnnnnn 283380nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 283440nnnnnnnnnn nnnnnnnnnn nncatgaaga caagtgtcca ggtgctgtgg gggccctggc 283500atgttagcaa gtgccctgca atggaggttc tgtcattggt gatggtaggt acataggtat 283560gtggtggtga ctgcaccatc tgatcattct tgtttcagca aattggctgt taagatctct 283620ctcaattcct gccaatccta atcctccatg cttcctagat ttctgtgagc tatgaatgct 283680ttctctacct aagttaacca gatctaatta tagacttttt tttttggtgc aacaaagggc 283740ccaaactagt acagcactac ttcagactta gtcttcctgg caatttgcct tcagggagct 283800agttctatag tgttttagaa taacatataa gacttgtggg atgggaagct tagtaaatat 283860ctatggtatc tgtttgccta gcatttcttt ccctgtgttt ctactaaggt acactcttac 283920tctggttttc ataaagacac atgacctaaa cttgatcaga caaaatattt tatcattact 283980ttgtgtcata gtgatataaa atgatataca cttacagggc gagtgtaacc taaggcttga 284040caatcagact tgagtctaga attgctttgt tgagagagat aggtaagggt aagaaagagt 284100ggtaggaagg aagagaagat gtagtagatg gtgttcatac ctcacccact tccttataaa 284160tctctttacc attttcatac acctgcatgt cttggttgga ggattgccct caggatgtca 284220gattcacttt gtacgtgcac atatttagct gaaagatctt agaaatttat gattccctgg 284280aagcagctct taactaatga ctaatatttg gttatcagga caaggctgag gggaaatctc 284340cattttggag tcctcctggg gaattgagcc aaggggattc acttgatttc acacctgtgc 284400accttagttt gtctgccttt ccttctctgg ccccatctct actccctcac cagattttcc 284460cagaaacact tcctaataaa ttcctttcaa ggtctgcttc taaataattt aatctaacat 284520accagagaag ccagaagtta tccctacttc cccgtggata acaatgcttg agaaaataaa 284580gccaacaagc agagagataa attgaatctg cttccgatca ctccagcctt tccaacagat 284640ggaatgataa attccctttt atacctaagc taaattgact ttgatttctg tcacttgcaa 284700tcaaaagata taattcatca gggtgatgca gagtgacccc tttccttgct taatccaaca 284760acaaatctca cagatatgat gtacatgata tcattgtgaa gggaaataaa tcagcaatgt 284820tttctgaaca aagttattag gaggccactg tcatatttac tgtgccttag atgccatgtg 284880cccaattcat gatttgtgat ttttgtacct ttcattagag cttattgtgc tgtttccaaa 284940cttttccaca gcataatcaa attaaaaaat tcagtctatc ttgatatatt ccatatcaca 285000tatcttgcct atgttaggaa agaaaaaaag aataaagagc aaaatctttc tgaaatgcaa 285060atatgatcat gtcagaccaa gtttaaggac cttcaatacc tcattcttgt cctcaagata 285120aggttcacac tccttaaaat agcctcaaaa ttcttcatta tgtggggtct gttgcaatct 285180ctgcccacag tcttcccatc caccccacat tctactcaca atgaaaactt ttcactcctt 285240gaatgacatg ctctttcttg gccctgaacc tttgtccttc ctctttctcc atatgcccac 285300accctcagat tctcaattca ctcagattat cacattttag gtttcagaac ttaactaagg 285360tgttacttct tccaggaagc cctctttgat gtcctgcctt cccccagatt cagattaagg 285420tttcctccta ggtgctccca gtattccctt tactttccta taattgtata tgtcaccttt 285480ttttgtgaat aaatagtgtt ccaattaaga atatatggcc tttgacagaa gattttgtct 285540gtctgtcgaa tgctatattt ctggagttta gcacagtgtc tgtctcacag agtcacaata 285600gtattgaatg aatgaatgaa tgaatgaatg agagatgttg aaagagaaga aataatatct 285660ttttgaacac cagcatagct ggtcattaaa ttccaagcag gaaacatctt cttagaggaa 285720atgtgctgat gggaaataga tgactacatc tattcctccc cagcatatgt ctccctgggt 285780atcatctctt tatgagagct aaagttaata accataaaat gtgtgctcag caacaagata 285840actcctgtga tttaacaaca ggaactacag tcatttagcc atgatacctg gcttccctcc 285900cagaataaga tacccaagtt ctcttaaaaa aaaaaagcaa ctaagtgtac ccacagctga 285960gagacttagg ttgatcactt aacctcttta ttgttatatt aatttatgga ttaatgtcca 286020gaggcagcta gctgtaaaga cttctcttca aaacaatttc atttttaaac ttgtctcatc 286080tagtagccag acagattaag gaataaggag actgatttat ggagcaaggt gtaagaatgg 286140aaatcagaag tttgtgaact atgaagaaaa ttgtaatata ttgtttctta gcaggagaaa 286200gactagacag cacctgtgta tcagattgaa gaacagcatt tattgagagg aaagttacca 286260tacacacaaa tggaaaacac acataacatg atgaccttca ccataaggta cagacagcca 286320cataaataga aggcactatc tatgtatgct cttttttatc tagtgaagta agacacttac 286380actctctcaa caagttgatt tgttttaagc tgtcaggttt gccctgacca ggatcactat 286440gctggatgtt atgtactcaa acaataggct aaccaatctc agaaatggaa gctacgacta 286500gtactgccct cctccgtgca gtatccttga ttgttttgct ggatatcagt catcatttct 286560taggagtatt aatcccaaat cagcataact gaggaaaaac atcaaaataa actttcttcc 286620aatcctcaaa aacttttatt agaaaagaat agaatgagga gaaaaagtat tacaagtctg 286680aggattgaca gtggcacgaa tcataatgca tgcttatgaa tgactgggta gaagaccagt 286740taggttgaac tgaagagctt gtgtaatggg gtattgaggg aaaaatgctg tagattgaaa 286800gactttttta aaaagtgtgg ttatagggta gaggcttgcc aaacttgtgc tttgggaata 286860agaagctgga aatgttgtac aggatacatg gcaggatgga gagagtacag gcaagtcata 286920tgtagtattc tatgcaaaaa tcatgatggt aagaaaaaaa gatgttaagg gtagcagaga 286980catttcaagc agatgcaaca aatgcttaca gcatggatgg tgaaatgtag ggctaaaatt 287040taaagatgat taatgaaagc ggatgacttg tcctttgaag tgaaattact tatttaaccc 287100aaacacatat gaatacctaa ttccggaaac atttgttgtc atcattctat ttatagttca 287160tgacttgcaa gaataaaata aggcatcttg cacaaatagc tttcttttcc tgacaaatct 287220tattgaataa gagattgtca gatataaaga gtttagtcat tacagatgga aatgagagct 287280gtactgctaa gactctgact tatttatctc atttattggc agtttcccaa aagggaagac 287340agagatatat taaggtttac ttatgttact tccctttgtg caatctagtt caccctcagc 287400ctgattcaag ggaggcagca ttatggtcag attaatcata tatttagaaa aaaaatgttc 287460tctaggttac ttagcattga aaaagttata tatttatatt aagctcttga actcagatca 287520gttaaaccca taaagaatga gcttgaaatg caagaaacac atcctggagt cattctgatg 287580ggattgctaa acttttcatc ctgggcatag agcagataaa aatgatgctt ctctgattta 287640acaaaagagt aacaggcata agacatctgc tgatgaaagg tgcaagcatt ttgacttaag 287700ggggtccctt tataatgttt gaatggaaac tgtgatacac cgtatcttat catctttgaa 287760agtgttcagc ctcctccaaa atgaagtgat tattctgttt ctagggaaag ataatgcact 287820ggtttaataa aagctgaatg ccaaagacat caatagtata atgatggtaa acttggcatc 287880tctgtccttt agttactgag agaaaaagtg tgttgtacat atttaggaca taataaatgc 287940caaacacttt gaaaggtaca ttcacacact tttcttattt taacctcata acagacttga 288000aagtattatc gttattattt ttacagataa agaaatgttc ctcatatcac atagtaagga 288060tcaggcctag aatttgaata caggtctgta cttttctaag ctatcttcta ttaacaaaca 288120gcgataaagc atgatgcttg agagtgtggt ccctaacagc cacttacggt gaacactggg 288180aaagaattct ctttgtttga ggtaaaaaga gccatggctc agcagaacac tgaggctcag 288240aggaaagcct agagttacta ctgtgatctc cctagatctg tgggaagtga gaggacttga 288300gaaacattcc cagctgaccc cacatttcca cacatatcag gaaacagtac ctgacagaat 288360gtgggttaga attaaatact agcgatgatg gtggagttgg aatttcatta acaaaaccag 288420atggaggtta ccctcacacc tctgaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 288480nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 288540nnnnnncccc ccaccccaca acagtcccca gagtgtgatg ttccccttcc tgtgtccatg 288600tgttctcatt gttcaattcc cacctacgag tgagaatatg cggtgtttgg tttcttgttc 288660ttgcgatagt ttactcagaa tgatgatttc caatttcatc catgtcccta caaaggacat 288720gaactcttca ttttttatgg ctgcatagta ttccatgttg tatatgtgcc acattttctt 288780aatgcagtct atcattgttg gacatttggg ttggttccaa gtctttgcta tcgtgaataa 288840tgccgcaata aacatatgtg tgcatgtgtc tttatagcag catgatttat agtcctttgg 288900gtatataccc agtaatggga tggctgggac aaatggtatt tctagttcta gatccctgag 288960gaatcgccac aatgacttcc acaatggttg aactagttta cagtcccacc aacagtgtaa 289020aagtgttcct atttctccac atcctctcca gcacccgttg tttcctgact ttttaatgat 289080tactattcta actggtgtga gatggtatct cattgtggtt ttgatttgca tttctctgat 289140ggccagtgat gatgagcatt ttttcatgtg ttttttggct gcataaatgt cttattttga 289200gaagtgtttg ttcatgtcct ttgcccactt tttgatgggg ttctttgttt ttttcttgta 289260aatttttttg atttcattgt agatttcaaa accgctcaac tacatggaaa ctgaacaacc 289320tgctcctgaa tgactactgg gtacataacg aaatgaaggc agaaataaag atgttctttg 289380aaaccaacaa gaacaaagac acaacatacc agaatctctg ggacacattc aaagcagtgt 289440gtagagggaa atttatagcc ctaaatgccc acaagagaaa gcaggaaaga tccaaaattg 289500acaccctaac atcacaatta aaagaaccag aaaagcaaga gcaaacacat tcaaaagcta 289560gcagaaggca agaaataact aaaatcagag cagaactgaa ggaaatagag acacaaaaaa 289620cccttcaaaa aattaatgaa tccaggaact ggttttttga aaggatcaac aaaatagata 289680gaccactagc aagactaata aagaaaaaaa gagagaagaa tcaaatagac acaataaaaa 289740atgataaagg ggatatcacc accgatccca cagaaataca aactaccatc agagaatact 289800acaaacacct ctatgcaaat aaactagaaa atctagaaga aatggagaaa ttccttgaca 289860aatacactct cccaagacta aaccaggaag aagttgaatc tctgaataga ctaataacag 289920catctgaaat tgtggcaata atcaatagct tgccaaccaa aaagagtcca ggaccagatg 289980gattcacagc cgaattctac cagaggtaca aggaggaact ggtaccattc cttctgaaac 290040tattccaatc aatagaaaaa gaaggaatcc tccctaactc attttatgag gccagcatca 290100ttctgatacc aaagctgggc agagacaaaa ccaaaaaaga gaattttaga ccaatatcct 290160tgatgaacat tgatgcaaaa atccccaata aaatactggc aaactgaatc cagaagcaca 290220tcaaaaagct tatccactac gatcaagtgg gcttcatccc tgagatgcaa ggatggttca 290280atatacacaa atcaataaat gtaatccagc atataaacag aaccaaagac aaaaaccaca 290340tggttatctc aatagatgca gaaaaggcct ttgacaaaat tcaacaacgc ttcatgttaa 290400aaactctcaa taaattaggt attaatggga tgcatctcaa aataataaga gctatctatg 290460acaaacccac agccaatatc atactgaatg ggcaaaagct ggaagcattc cctttgaaaa 290520ctggcacaag acagggatgc cctctctcac cactcctatt caacatagtg ttggaagttc 290580tggccaggac aattagtcag gagaaggaaa taaagggtat tcaattagga aaagaggaag 290640tcaaattgtc cctgtttgca gacgacatca ttgtatatct agaaaacccc attgtctcag 290700cccaaaatct ccttaagctg ataagcaact tcagcaaagt ctcaggatac aaaatcaatg 290760tacaaaaatc acaagcattc ttatacacca acaacataca aacagagagc caaatcatga 290820gtgaactccc attcacaatt gcttcaaaga gaataaaata cctaggaatc caacttacaa 290880gggatgtgaa ggacctcttc aaggagaact acaaaccact gctcaaagaa ataaaggagg 290940atacaaacaa atggaagaac attccatgct catgggtagg aagaatcaat atcgtgaaaa 291000tggccatact gcccaaggta atttatagat tcaatgccat ccccatcaag ctaccaatgc 291060ctttcttcac agaattggaa aaaactactt taaagttcat atggaaccaa aaaagagccc 291120gcatcgccaa gtcaatccta agccaaaaga acaaagctgg aggcatcaca ctacctgact 291180tcaaactata ctacaaggct acagtaacca aaacagcatg gtactattac caaaacagag 291240atatagatca atggaacaga acagagccct cagaaataat gccgcatatc tacaactatc 291300tgatctttga caaacctgag aaaaacaagc aatggggaaa ggattcccta tttaataaat 291360ggtgctggga aaactggcta gccatatgta gaaagctgaa actggattca ttccttacac 291420cttatacaaa aatcaattca agatggatta aagacttaaa cgttagacct aaaaccataa 291480aaaccctaga agaaaaccta ggcattacca ttcaggacat aggcatgggc aaggacttca 291540tgtctaaaac accaaaagca atggcaacaa aagacaaaat tgacaaatgg gatctaatta 291600aactaaagag cttctgcaca gcaaaagaaa ctaccaacag agtgaacagg caacctacaa 291660aatgggagaa aatttttgca acctactcat ctgacaaagg gctaatatcc tcctccttct 291720tcttcttctt ctccttcttc ttcttcctct tcttctcttc ttttttttct ttttcttcct 291780tttcttctcc tctctctctc tctctctttc ttttgctgct tggtaactcc taagaaattt 291840tagctcagaa aacacttatt tgatacattt gtatgtgatc tttacttccc ttctgttctt 291900tctttcctct cttccttcct gtaagttttg ccagtttcag aggcagaatc aaatagaaaa 291960agactaccct tcatgaagcc agtcttaaaa gagagactgc tgggactctg ttgaagtgga 292020agactttttt aaccaagatg acaaggaaag cattgctaaa ggtagaatca aaatcaaagc 292080agtgtttaag agggcacatt cccatgaacc tgggaagttg atgtggatgg tcaattctga 292140aaagtgaggt gaggatgaaa ataggaggat gaaaagctct gggaatatca agagtaagac 292200aatgaatgtt acaggaataa gtcaggacaa gctaaagttc tggaaagaag agagtgctga 292260tgctctaatt tttatgtggc atttggtatg cagtgtaggt gattatgcaa agaaatttta 292320taggactggg tatttatttt gtattttgtc aaatatcttg tgcatcaaaa agcttcttat 292380ggatgaattc tacttataac atattgagcc tttctttgtt gttgttttga ctttgaaaaa 292440tatttgacct actcataaag aaaatatatt gacagttatt ttccccctag agggagtata 292500tgtgtaggaa ctagcaagag tgaagtcata attttggacc taatcttagg aataccatga 292560tgtgtcattt ctctgacttc taaaattgaa gtcaaagaga gatgcaggat cattttctat 292620tctttggcaa aaggtattat agaaatgcag agtatcggcc tatcatttac acagccttga 292680actgtgctgt cctcagaata caaaacagaa aattgcagtt ggaggctatt gcaactgact 292740tcctctgatt catgaataat ttccattcaa ctcaaatagc agaaataacc ctcaataaga 292800aagaggcaca tgatagctcc acctatcaat ccatgcaggt gaacataaaa ctaccaactt 292860ttacatggac agatgtgatt tgttatctcc atcctagtgg aaaaataaaa tcaaatttat 292920actttaccgt gcttaagaag acaccgactc acggttaatc gctgtgattc attcctaagc 292980cattgctctt ccttcaaagc tgaagcatga cccagtcagg ttcactacta attcatgtag 293040cagccacatt tacaactgaa atgcaagggt gggggttcaa ggtggggtga aggtggaggg 293100gaggggaggt gagggggaag tagctctagg aaatatcaga tgattatcta cttatctagt 293160tatcagatga ttatctgtta tctagttaat agaaaagtga taggtaacca aagagattaa 293220atgtaagaca catatttgta attataggtt acaatttgta attgctattt gttatttgta 293280attgtaggtc aatccagaaa tggagacaat tcctggctgc atacatccac ataatgtaat 293340ttactgcata gattattaac tgcatagtac ctagttcact gcaataaact acaataattt 293400acaataaaca tttttttcca aaagcatcaa gttatatttt tgctgttgtt tagatgtgtc 293460tggtatataa atggaacaac attgctctaa gtagaattgt ttctttaaaa gtaagatccg 293520ggaaacgttc ataactactc tgcatctctg ttacaatagt aagggtgcag aacatgtcag 293580ctaaagggtg tctagtcccc agcatgatgc ctggcagtaa atcaaggatg agccttcact 293640gcttcaagtc ctttcatcta gaatgcttca gtgagttttt atggggaaat catttccccc 293700tctttcttaa gatcatttat catttagttg tataaactga tatttacatc tgtcacaaat 293760gctcaaccct atcccagagc atattaaggg aaacttgtgg aatatttatg tgttatgtgt 293820atacaaaaag aggttgtcta caaaacctct ataaaagaca agttgccatt tcccatacct 293880tgggggaata gtagaagcac ttttctcttc tcccctatgc tgaatttact gccattttct 293940tgccttcttt ataggaaact ctaccttttg tgtatttaaa acactgctag gtgtttgtgg 294000aaaacagaag aagaaacttg ctactttcta atttttgttg ttgagaagac acaataatat 294060ggtattttat agttagggtt gtcaaaagca aatctgtgct tcgccacata acagcaggac 294120ctttggaaaa ttatttgact gttctaaatg ccagttccct ctctgtaaca tggaaacata 294180atagcattga ggtctcaaag tttctgtaca tgaaataatg tacatgaagc acattgtaca 294240ttgcctcttt aattctcaaa ccactccatg aagtgaaact attattattt ctcattcaac 294300agatttgttc attaaatttc ccaaggtcac tctgttaact agtagtggac atggtattca 294360aacccagctc tgcctgactc tagggcctga actccttatc actcatgaga ctcctcccta 294420ggtacttcac tagtttctaa caaggaagga tttaagtcac cgtcagagct ctgctatata 294480atcaattctt gtatttgtga tatttgtaat ttggtctagc cttattgtat agaaacctgt 294540tgtattagtc cattcccaca ctgctatgaa gaagtaccca agactgggta atttataaag 294600aaaagtggtc taattgactc acagttcctt atggctgggg aggcctcagg aaacttacaa 294660tcatagcaga aggcacctct tcacagggct gcagaagaga gaatgagagc aagtagggaa 294720aatgtcaggt gcttataaaa tcatcagatc tcatgagact cactatcacg agaacagcat 294780ggggggaacc acccccagga tccaattgcc tccacctgtt tctgcccttg acctgtgggg 294840tttttaagga ttataattca agatgagatt ttgggtgggg cacagccaaa ccatatcacc 294900tgtctccagt tttcatacca tactacagat acagccattt cacatccaat cgtgggttat 294960tgttttaaaa tcacacatct aaaaatctcc tcaagcattg ctctaatttg atatctaaca 295020ggtaattccc aaaggctagc tgcagtgaag gttctggggc tcagggtagg cagagcaact 295080cagcagctgg tataaaatgg gctcttctcc aggtgtcctg caggttacac ttatattcac 295140aaatgttaat ccagctgcta cctgtttgaa attgctaata gagttataac agattacctt 295200tcttcaagcc aataccagaa aattcaaacc agagacaaaa ttagtcctgc aggtactggc 295260agcttaataa tagcttatcc ctgaggatct caatctttgt ggtctcagaa aaagtgcttg 295320taaaaagcat tgaagatcca aaagtagtta tgtgtgttaa ttatgtatta ctgggctcca 295380tattaaaaat taaaactgat acttttattt gaaataacaa taataaaccc attacatgtt 295440agtataaatt aatgttaaca taaacacctt taaataaaaa atatattttt caaaacaaaa 295500aaaattagtg aggagtatgg cgttgttaaa catttttgca aatctccttt atggcttaat 295560aaaagataac tggattttta catctgcttc tgcattcaat ctgtcacagt attacacttc 295620ctgtagattc tggaaaatgt cattcttcac tcatgaggga ataagaagga aagaaaatat 295680aacattttag aatgatgatt aagatagttt tgaacttaca gaatccctgg aaagatcttg 295740gggacataca agagtctcta cccgacattt tgagaaccac tagctagaag tatacttgat 295800atagctagaa ataaacttga tgccataagg aatgtcctac tgatgcagtt ggtttgattg 295860atagttcaat aatattcatg actaaaatca ttcaagcttt aaaaagtaga ctatttagta 295920acacaaggtt tgaatttgtg agttccaaaa cttttacaca agtagtggga aggtctggga 295980cctttgtcat tatagctttt gcagatgtca gaaataatag tatttctttc ctatgatctt 296040tgttcactta gagtgtgatc tgactggtgt caaaagtcac ttaaaagact ggttgtgaga 296100ttcaaatata tattccttcc aagagtattt actggacagt gaggtaccca tcttcaaagg 296160atccagcagg catctggtgt gtaaacaagt ctgtggtggc ccagttggct ttcttttcca 296220caggttctga gggagtccac atctccccct tttttccact gtgtccttta gcggaccact 296280tcagcacaca tagcctggtt ctcttgcatt gtaacaggcc cacagaatct ctgggcactt 296340atgctttcct gcttctgagc atattcctta tagtttccat ggttacattg gtgaagctcc 296400aagtagtcca gatggctgca gaacacagtt ctttgattac ctctcctcag agtacccacc 296460tgctttcttc cgtcccattt gattttcttt catccttggc ccaaggagat ctttaacagc 296520ctgcagttcc tagtgacctt gttaatttgt gaagctcatt ccaaatagtc tccattagta 296580atattcagct aactctgcag ttagttaggc atataataaa ccttgaaaat aagtctgata 296640ccaatgttat caatatttat aaattttctt aaatcttata aaatcatcat tattgctgct 296700atgcacaagt tcttaaaatt taacatattc atatcatgca tcttgcaatt tgtaggactt 296760tcttagaact actgtgatat aaatcataac ataaaatatc agttatgatg tatcaataag 296820tgataagcta acatatttgt aactattttc agtttaaaaa gtcctttcat agacattatt 296880tcatttaatc cccaacaaat gctctttcag atagttacct cttatgccta ttttacaaat 296940gagaaaaaga aatgaggcaa tgggaggtta attaacttgt ttgagtaatc aacaggctgt 297000tttccaatta tgggtcttca gattaacaac ttagcccctt tctgttatac tggtgttttg 297060tgtatttaag gtatttccat attatttgcc atatccatgt gctcgaattt gctgtatcaa 297120ataaagctat ttaattctag ttggaaactg ttgcctacta aaaaaacaaa aacaaaaact 297180tagggggaaa atagcatgag gttgagaagt tgttaaagtc ataccaatac caaactgtgg 297240cttcctcttt aagaactgaa aggaaattga ataaggaata actttaacaa tacactacgt 297300ataatccttt taatatctac ccatgcttgc tcattgaatt ttgagcactg tcttcaatca 297360ttctgaatcc cttgcctcaa tgagttccaa gtctcccata aagcaggtga tcaaccaaaa 297420tcttttaaaa ctgtttgaaa gtggagaccc ccccaaaagg aaatcaatat gtgactttca 297480gcataaattg tggcatggca tatttgaaaa agtaacggaa gtaaaacaca tttagttagc 297540gaagagcatc aagtttatca tagaagtgat tgttgagaat gtcacccaaa ctcacccaaa 297600aattcactgc aattgtctag gaggaaggaa gtggagacaa catatagctg attcaggtct 297660catttcaaag agcttcttta taggagagaa acatgtatat caagttagga ttccagttag 297720gaacaaagat aggatgagcc catgtttccc aatctcttct gtgagagaac actgtaatac 297780tggaataaac ataaagaggc tgcggtagct gtaagaactt ccttagaggc taagagttcc 297840tgagtggctg ccagagtctg gggatcaata tctctggaaa cttgcagatc acttgtagct 297900cactaggcta cagcaccaca ttggtgggaa gctctgctgt aggaaactgc tgagaggacc 297960atggaataag aagaaaaaaa catagcccct gacaacaaag aattaattct ttcttccaga 298020aaaaaatgta caaagcattg atagtggcag tggcctgtct ggagcagcca ctgtgaggac 298080accggctgta gcaggggagt catagccagg gctgcatgct ccatggagct ggcaggggca 298140gggcacaggt gatcccagtg ggagtcccac accctacaga gttggcagag tggaagccca 298200cactcctggg tgaagctgca gtcactcagc tgtggctctg gacctgggca tccctgtgct 298260ctcagggaac ccaggaagct cccagggacc caggaagctc cctgcccctg caggctcaga 298320agtgcttctc ccactccctg gcctctccct gctgccagca cccactatgg atgtgtctca 298380acagccaagc ccaggcactg ttgtgacccg gctgggtgtg tgcacactca ggatggtgct 298440gacatgccag ccccctgctt cctcgacccc cccaaaaaat aacctctaag gctgaaactt 298500tgggtgccaa tgagcacagg agggaggttg ggggactgag ggtggctcaa ggcaggcctg 298560cagttgcccc ttgatgcaga cagcctgggc accatggatg gcatgttgat ggtggcaaaa 298620ggcagaaagg ttcctaggca gcaaggggtg ggtccccact gaaaccccac cttcaagcca 298680gggatggcct aaagtctggg ggctgggctt ccagttccag gtaaagtacg agaccctgag 298740tgagaacttc cttgatgcct ttcggccaat cagatggtgc tttttccagg cctgcccatg 298800gccgctcatg gatcaatcag catgcgcttc caccaaagaa ctagtcagca tgcacatcct 298860tcattctgag cccataaaaa ccccacacac agccagactc acacacttat tgggactacc 298920tgcctgtgga taggagcttc ccactttagg tctcctctgc actcgttggg actacgtgcc 298980tgtggatagg agtaaccact tcaggtttcc tctacactcg ttgggactat gtgcctgtgg 299040ataggagcta accactttag gtctcctctc cgctgagagc tgttctgtca ctcaataaag 299100ctgctcttca acttgctgac cctccagttg tccatgtaat ttcattcttc ctggatgctg 299160gacaagaact caagacctgc tgaacagagg gagttaaagg agctgtaaca cattcctggc 299220tggcttgctg agctgcaggg ataacatgct ctcggactgt ggtagtgaag aggggtgacc 299280cttctggggg ctcagacctc aggatttccc cagccagagc tgctgtaaca ctatagtcat 299340cctgccctcc accagagcca ggcagccatc ccatgcaacg ggaagcagca gcagggctag 299400gagccatggg ctggagaggg gcagtgggac tgaaagagtg gtaacacaaa caggctgaaa 299460catacccccc cttccatttg ccgcgctgag ggtgacgaga aggagagaag agctgtggcc 299520cttctgggag tccagacttc aggctccctg agccagggct gtgacatgct gtaacaccct 299580tttgagggct ctgcggttcc tcgtgtctcc aagcttttga tcgctaccat gttcccctca 299640ccagtcagtg gtaccctcag tggaaaccac ttacaatacg tctggcccag ctgtaacctc 299700atacaaagct ggtgcctgtg ccagcgcctg gagctgcctg ccctgctgca gcagccagca 299760tgcctggctg tccacagtgg ccagaccccg cactcacaca cactttgctg caccatacct 299820ggcttgccct tgagaggtgt agaaatgcag catctcaggt tgctgcctgt ggtgtgagcc 299880aagcacagac tcccaggtca agcatgtgga acaagcccag cgggcacaag caaatctcaa 299940gcagagttgc tgctggccac agaggtttcc agctggagaa gtgacaccca aaggaccctg 300000tgactgcatt ttgtatgagg aaggcactgt aatagatact atggacataa ggcagatttg 300060tattaaacag aattttcagt tttaccttgg agaaggaaaa cattgaccta caacttgtga 300120cacatagtga cagacatttt aaatttctca gagcttacaa gtattcaaga aatgactact 300180atatatttct ctgccaaatt aaatttcttt tccacactca atttttatat actttacagc 300240cattaatttc atggtatgca ttttacttct tccaattgcc tcatcaaata atatataaac 300300actttaatct aaatttattt ctctctttcc aggaaaacag tttattgggt actaataata 300360taaggggtat tccacttgtg ctttatataa actattttaa acttacatca accccacaaa 300420actggaataa tcgcccaatt ttacagatga gaacattttg gccaaagaaa aaaagtaaat 300480aatagacaat aacccagcat taaaatggag gcattcctga cttaaaagcc aatccctttt 300540cactgccata tatttcagtt ttggaatcta tctccattaa tctttgtatt gagttaataa 300600aacaaatata gaaaattatc cactgttaga ctaatgttct tcctctattt acttcacaga 300660ctgacagatt gataatcaca tacatatacc tatacatatg tgtattagtg cctttttatg 300720ctgctaataa agacatacct gagactgggc aatttacaaa agaaagaggt ttaatggact 300780tagagttcca catggctgga gaggcttcac aatcctggtg gaaggcaaag aggagcaagt 300840cacattttat atggatggca gcaagcaaag agagagcttg ttcagggaaa cttccatttt 300900taaaaccatc agatgtcatg agacccattc actatcacaa gaacagcacc agaaagaccc 300960acccccataa ttcaatcacc tcccactggc ttccttccac aacatgtagg aattgtggga 301020gttacaattc aaaatgagat ttgggtggag acacagccaa accatatcat tctaccctgg 301080cccctcccaa atttcatgtt ctcacatttc aaaaccaatc atgccttccc aacagtcccc 301140aaaagtctta actcatttca aaagtccata gtccaaagtc tcatctgaga caaggcatgt 301200cccttgcacc tatgagcctg taaaatcaaa agcaagttag ttactttcta gacacaatgg 301260gggtatagac attgggtaaa taacagcagt tccaaatgtg agaaattggc caaaacaaag 301320gggctacagg ccccaagcaa gtctgaaatc cagcagggca gtcaaatttt aaagctccaa 301380aattatctcc tttgactcca tgtcctggat ccaggtcaca tttatggaag taggttccca 301440tggtcttggg tagcttggcc cctgtggctt tgcagggtac agcctccctc ctggctgcct 301500tcatgggctg gcattgagtg tctgcagctt ttccaggcat atggtgcaag ctgtcagtgg 301560atctgccatt ctggggtctg gaggaaggtg gccttcttct cacagctcca ctaggcagtg 301620ccccagtagg gactctgtat gggggctcca accccacatt tcccttccac actgccttag 301680cagaggtact ccatgagcac cctgcccctg cagcaaactt ctgcctggac atctaggtgt 301740ttccatatat cctctgaaat ctaggcagag gttcccaaat ctcaattctt gacttttgtg 301800catctgcagg gtcaacacca catagaagct cccaaggctt ggggcttgca ccctctgaag 301860ctatggctca agttgtacct tggccccttt tagtcacagc tggagtggct gggatgcagg 301920gcaccaaatc actagactgc acacagcaga ggggacctgg gccaggccca tgaaaccatt 301980ttttcctcat agccagtgat gggaagggct gccatgaaga cctttggcat gccctggaga 302040cattttccag gccagtgatg ggaagggctg ccatgaagac ctttggcatg ccctggagac 302100attttcccca ttgtcttggg gattaatatt cagctcctca ttacttacac aaatttctgc 302160agcctgctag aatttctcct cagaaatgag attgtctttt ctatcgtgtt gtaaggctgc 302220aaattttcca aatttttatg ttgtttccct tttaaaactg aatactttta acagcaccca 302280agtcacctct tgactgcttt gctgtttaga aatttcttct gacagatacc ctaaatcatc 302340tttctcaagc tcaaagttcc acagatctct agagcagggg caaaatgcaa ccaatctctt 302400tgctacaaca taacaagagc cacctttgct ccagttccca acaagttcct catctccatc 302460tgagaccacc tcagcctgga ccttattgtc tatatcatta tcagtatttt ggtcaaagcc 302520attcaacaag tctctaggga gttccaaact ttctcacatt tgcctgtctt cttctgagcc 302580ctccaaactg ttccagcctc tgcctgttac ccacttccaa agtcgcttcc acattttcag 302640gtatctacag cagcacccac tttattggta ccaatttact gtattagtct gttttcatgc 302700tgctgataaa gacataccca agactgggca atttacaaaa gaaagaggtt tagtgaactt 302760acacttccac atggctgggg agatctcaca atcatggtgg aaggcaagga gaagcaagtc 302820acatcttaca tggatggcag caggcaaaga gagagctttt gtagggaaac tcccattttt 302880aaaactatca gatctcgtaa gactcattca ctatcatgag aacagcacag gaaagacctg 302940cccccatatt ttaatcacct tccaccagtt tcttcccatg acatttggga attgtgggag 303000ttacaattca agatgagatt tgagtgggga cagaaccaaa ccatatcaac atgttaagaa 303060tataactttt aataatatac tttgaaaata taaaatcatc ttttcatata aaaattaatt 303120ttggggcaat aggagtagat ctgtaaaaac tcttcaatta ttaagaaatg tatatttata 303180ggatcccaat aaaatccttg tctgatgtac tgaaagtcac atattgtttc cttttggggg 303240atctccactt tcaaatagct ttaatagatt ttggttgtgc acttgcttta taggagactc 303300tgaactcagg attcagaatg attgagaata gtgcctaaaa ttcattgagc aagttcttac 303360tcagtagatc tgaggtaagg cctaaaatgc tgcatttata gcaagctcac aggtgaggct 303420aaaactgctg gtcctggcat cacactctga ggagtagggg tccaggatat acgcattaaa 303480ctatggtcca aagaccagtg ctagtcagga aactgttggt tccttatctg caatgagttt 303540atggtagaga ctgagggtga gcatttagac aattttagca ctttgtggag taattttatg 303600tttgttaaac ttaataaaat aaaaaaattg gggcttgctt ctatttcata tgactttttt 303660aaaaatttca tctttccagt aattattttt atcatatttt ataaaaatat tggtctgtga 303720taggtggaag atgatatata taactagtta tatataatta attatttata atatgagtat 303780ttattacata tataatatat tactatatat tatatattag ttatatataa tcaattaatt 303840ttatatagct agttatatat ataacttgct atgtatataa ttatatacag ctggttttag 303900atataattat atataatatg taacataaat atataataca tttaatatag ctagctatat 303960ataattaaca tattatatag aacacatata actagtttat atataacata cataactact 304020tttataaatt aatatatatt atatataaca tatataaaac tcattttata aaatatatat 304080aaaactagta acctttgtgg tgaaggacta ctatatgtgt atagcagtcc tatatgtata 304140tatatgtacc atatgtatat agtagtcctt caaaaatgtg ttaatacata tataccatag 304200aatactatgc agtcataaaa aagaatgaaa tcatttgtgt tgaaggatta gtcatatata 304260tataaaacta gtccttcacc acaaatgatg taagaagcat gagtctagga caatgcccct 304320ggctcatgaa acttacaata caatgggaga actatgacaa gcatcaaagg taccaatgca 304380gaaagaaaca tgtagccaca atataatctt aaggtttgct tacaaaggtg tgtttctcca 304440tcaaacaaac actccattca ccgaaagaaa acagcttatg atgcatcctt aataaagaat 304500gcttacattt gcctttttag cttgacatgt attcttttct gataccaatg cagaaagaaa 304560catgttgtca caagacaaca caaaggagaa tgtgatgaag accatttaag aaatgaagag 304620ttctaagcgt tctgaggact gaacagaagt aaaaggatcc agcattcgtg gagtatctgt 304680ttatgtacac acataatgtt aagtccttaa catttaagcc acataaaatc ccattttgaa 304740aatgaggaaa ttgaagacca gggagtttaa gtaactaact gaagattcag aaggagcagg 304800gctggaattg agatctagct ataatgtgta tgtatgaact gtagatcctc atatttttga 304860acaccctctc tacattctgc taggatccca cactgtgagt ctcattttcc taaggtgaac 304920tccagaaact agatttccag acttcattac agtgagggtg caggtacatg atgtaggttt 304980tgtaatatga agactggaca gaccagagct tggaaacata ggcaccagac tatggatcag 305040acagaagaca ctgttggttt ctgatgtggg ttgaattgtg tccctgtccc caccccttag 305100gttcatatat tgaaatctta accctagtaa ctcagaatgt gacttcattt ggaaataggt 305160tcattgcaga tgtgatttgg attgagcagg cccctaatcc aatgtgactg gtgtccttat 305220aagaaggaga tttttttttc attttttttt gtttatttct atttttataa tctcaacttt 305280tattttagat tcagagggta catgtgcagg tttgttacat gggtatattg cattatgcta 305340aagtttggga catgaataat cctgtcaccc agatagtgag catggtactc agcagttagt 305400ttttttaacc tctgtccccc cattagtctc cagtgtctat tattgccttg tttatatccg 305460taagttccca atgttggcca ggcacggtgg ctcacacctg taatcccagc actttgggaa 305520gccgaggcga gtggatcatg aggtcaggag ttcgagacca gcctggccaa catagtgaaa 305580ccccgtctct actaaaaata caaaaattag ctgggcatgg tggcacacac ctgtagtccc 305640aggtactggg gaggcagagg caggagaatt gcttgaaccc aggaggcgga ggttgtggtg 305700agctgagatc atgccactgc actccagcct ggtgacagag caagtctcca tcaaaaaaga 305760ataataataa caaaaaataa ataaatacca aagttcccaa tgtttaactc ccacttgtaa 305820gtgggaatgt gcagtgttgg aatttctgtt cctctgttat tttgcttagg ataatggcct 305880ccagctgcat caattttgct gcaaatgaca tactttcaat cttttttatg actgcatatg 305940tattccatgg tgtatatgtt cattttcttt ttccagtcta ccactgatgg gcacctaagt 306000tgattccatg tctttgctat tgtgaatagt actgtgataa acatttgagt atgtgtcgtg 306060ttggtaggat gatttatttt ctttggatat atacccagta atgggattgt tgggttgaat 306120ggtggctctg tttcaagttg ttgaggaaat ctccaaactg ctttccacag tagctgaact 306180aatttttact cccaccaaca tggataagtg ttcccttttc tctgcagctt ctctagtatc 306240tgttattttt tgacttttta attatagcta taatgaagta aaacacccct cagcaaatgc 306300aaaagaatgg aaatcacaat gaacagtctc tcagaccaca gtgcaatcaa attagaactc 306360aggattaaga aattcactca aaaccactca actacatgga aactgaacaa cctgctcctg 306420aatgactact gggtaaacaa tgaaattaag gcagaaataa ataagatctt tgaaaccaat 306480gagaacaatg acacaatgta ccaaaatctc tagcatacag ctaaagcagt gttaagaggg 306540aaatttatag cactaaatgc ccacatcaga aaactggaaa gatctaaaat cgacacgcta 306600acatgacaat taaatgaact agagaagcaa gagcaaacaa attcaaaagc aagcagaaga 306660caagaaataa ctaaaatcag aacagaattg aggaagatag agacacgaaa aacccttcaa 306720aaaaatcaat gaatccagga gctggttttt tgaaaagatt aacaaaatag atagaccact 306780agccagacta ataaagaaga aaagagagaa gaatcaaata gacacaataa aaaatgataa 306840aggggatatc accactgatc ccacagaaat gcaaactacc atcagagaat actataaaca 306900cttctatgca aatgaactag aaaatataga agaaatggat aaagtctggg acacatacat 306960ccttccaaga ctaaaccagg aagaagtcaa atccctgaat agaccaataa caagttctga 307020aattaaggca gtaattaata gcctatcaac caaaaaaaag cccaggacca gacagcttca 307080cagccaaatt ttaccagagg tacaaagagg agatggtact attccttctg aaactattcc 307140aaacaataga aaaagaggga ctcctcccta actcacttta tgaagccagc ctcatcctga 307200taccaaaacc tggcagagac acagaaacaa agaaaatttc agggcaaaat ctctaatgag 307260tatcgatgaa aaaatcttca gtaaaatact agcaaactga atccaacagc acatcaaaaa 307320acttattcac cacaatcaag ttgacttcat ccctgggatg caaggctagt tcaacttaca 307380caaatcaata aatgtaatcc atcacataaa cagaaccaat gacaaaaacc acatgattat 307440ctcaatagat gcagaaaagg ccttcgataa aattcaacac ctctttatgc taaaaacact 307500caataaacta ggtattgatg gaacatatct caaaataaga gctatttatg agaaacccac 307560agccaatatc atactgaatg ggcaaaagct ggaaacattc gttttgataa ccagcacaag 307620acaaggatgc cctctctcac cattcttgtt caacatagca ttggaagttc tggccggggc 307680aatcaggcaa gagaaagaaa tgaaggtatt caaataggaa gagaggaagt caaattgtct 307740ctgtttgcag atgacatgat tgtatatttc taaaacccca ttgtctcatc ccaaaatctc 307800cctaagctga taagcaactt cagcagtctc aggatacaaa atcaatgtgc aaaaatcaca 307860agcattccta tacaccaata atagacagag agctaaatca tgagtgaact cccattcaca 307920attgctacaa agagaataaa acacctagaa atacaactta caagggacgt gaaagccctc 307980ttcaaggaga attacaaacc actgctcaag gaaataagag agaatacaaa caaatgaaaa 308040actttccatg ttcatggata ggaagaatca atatcatgaa aatggccata ctgaccaaag 308100caatttatag attcaatgct attcccatca agccaccatt gactttcttc acagagctag 308160aaaaatctac tataaatttc tttttttctt tttaattata ctttaagttt tagggtacat 308220atgcacaacg tgcaggtttg ttacatatgt gtacatgtgc catgttggtg tgctgcaccc 308280attaactcat catttaacat taggtatatc tcctaatgct atccctcccc cctcccccac 308340cccccaacag gccctggtgt gtgatgttcc ccttcctgtg tccatgtgtt ctcattgttc 308400aattcccacc tatgagtgag aacatgcggt gtttggtttt tcatatggaa ccaaaaaaga 308460gcccatataa caaacacagt cctaggcaaa aagaacaaaa ctggaggcat catgctacct 308520gacttcaaac tatactacaa ggctagagta accaaaacag catggtagtg ataccaaaac 308580agatatatag accaatggaa cagaacagag gcctcagaaa taataccaca catctacaac 308640cacctgatct tcagcaaacc tgacaaagac aagcagtggg gaaaggacaa cctatttaat 308700aaatggtgct tggaaaactg gctagccata tgcagaaaac tgaaactgga ccccatcctt 308760ataccttata caaaaattga ctcaagatgg attaaagatt taaacataaa acttgaaaca 308820taaaaaccct agaagaaaac taggcaatac cattcaggac ctagccatgg gcaaaaactt 308880catgactaaa acaccaaaag caatgttaac aaaagccaga attgacaaat gggatctaat 308940taaactaaag agcttctgca cagcaaaaga aactatcatc agaatgaaca gggaacctac 309000agaatgggag aaaatttttg caatctatcc atctgacaaa gggctaatat ccagaatcta 309060caaggaagtt aaatttacaa gaaaaagaca accctatcaa aaagtgggca aaggatatga 309120acagacagtt ctcaaaagaa gacatttatg cagccaacaa acatatgaaa aaaagctcat 309180cattgctggt cattagagaa atacaaatca aaaccacaat gaggtatcat ctcacgtcag 309240ttagaatggt gatcattaaa aagtcaggaa acaatagata gggaggatgt ggggaaatag 309300gaatgctttt acactgttgg tgggagtgta aattagttca accattgagt aagacagtgg 309360tgattcctca aggatctagc atcagaaaca gcgtttgacc cagcaatccc actactgggt 309420atatacccaa aggattatac atcattctgc tataaagaca catgcacgtg tatgtttatt 309480gcggcactgt tcacaatagc aaagacttgg aagcaaccca aatgtccatc aatgataaac 309540tgtgtaaaga caatgtggca catatatacc atggaatatt atgcctccat aaaaaagaat 309600gagttcatgt cctttacagg gacatgaatg aagctggaaa ccatcagtct cagcaaacta 309660atacaagaac agaaaactgg gccgggggca gtggctcacg cctgtaatcc cagcactttg 309720ggaggctgag gcgggcggat cacgaggtca ggagatcgag accatcctgg ctaacatggt 309780gaaaccctgt ctcttctaaa aatacaaaaa attagccggg cgcggtggca ggcacctgta 309840gtcccagcta cttgggaggc tgaggtagga gaatggcgtg aacctgggag gagaagcttg 309900cagtgagctg agatagagat tgcaccactg cagtctggcg tgggtgaaac agtgagactc 309960catctcaaga aaaaaaaaaa taaagaacag aaaaccaaac acggcatgtt ctcactcaca 310020agtgggagtt gaacaataat aacacatgga cacagggagg ggaacatcac acactgggtc 310080ttgttggggg tagggggcaa ggggagggag agcactagga caaataccta atgcgtgttg 310140ggcttaaaac ctagatgagg gttgatgggt gcagcaaact actatggcac atgtatacct 310200ctgtaacaaa cttgcacatt ctgcacatgt atcccagaac ttaaagtata ataaaaaaga 310260taataataat agctataatg actggtatga gatggtatct cattgtggtt ttgatttgca 310320tttatctgac cattagtgat gctgagcacg ttttcatatg tttgatggcc acttgtatgt 310380ccttgttttg agaagtgtct gttcatcctt tttgcccact ttttaatggg gttgtttttt 310440gcttgttgat ttaagttcct tatagattct ggatattaga cctttgtcag atgcatagtt 310500tgtgaatatt ttctcccatt ctataggttg tctgtttact ctgttgttag tttctgttgc 310560tgtgcagaag ctctttggtt tacttagatc ccacttgtca atttttgttt ttgttgaaat 310620tgttttaagg ccaatgtcca caagggtgtt tcctaggttt tcttctagaa ttttctttct 310680ttctttttat ttttttttta tttttgagat ggagtgttac tctgtcaccc aggctggagg 310740gcagtggcac aatctcgatt cactgcaacc tccacctccc aggttcaagt gattctcctg 310800cctcatcctc ccaagtagtg ggattacagg cataagacac catgcccagc taattttttt 310860atttctagta gagatggggt ttcaccatgt tggccaggtg ggtgccgaac tcctgacctc 310920aggtgatcca cccacctcag cctcccaaag tgctgggatt acagatgtaa gccaccatgc 310980ctagcttctt ctaggattct tacagtttga ggtcttatat ttaaatattt aatctgtctt 311040gagttaattt ttatatatgg taagagaggt ccagtttcat tcttttgcat atgtctagac 311100agcaatccca gcaccaatta ttgaataggg agtcctttcc ccattgcata ttttcatcaa 311160ctttgtcaaa gatcagatgg ctgtaggtgt gcagctttac ttccaggctc tctattctgt 311220tccattggtt tatttgtctg tttttatact agtaccatgc tgtttggact acttagcctt 311280ataatatagt ttgtagtcaa ggaatgtaat gtgatgctta cagctttgtt tttgttgttg 311340ttgttgttgt ttgtttgttt gtttttgctt agattgcttt ggttatgcag tctctttttc 311400agttctatat gaattttaga atagtgggaa ttttttttac tctgtaaaca ataacattgg 311460tagcttaata ggaatagtgt tgaatctgta gattgctttg gtaagtatgg ccattttaat 311520aatattaatt cttctaatcc acgagcatgg aatgtttttt atttgtttgt gtcatcttga 311580tttcttttag ctgtgttttg tagttctcct tgtagaggtc tttctcctcc ttggtttaat 311640gtattcctag gtatattatt ttggggaggg ctattgtaaa tggaactgca ttcttgattt 311700ggctctcagc ttgaatgtta ttggcataca aaaatgctat tgatttttgt atgccaataa 311760cattgatttt gtatcctgaa actttactga agttatttat gagttccagg aggctcttgg 311820tggagtaata gagtaatatt gttagtaaag agagatagtt cgacaatttc tttttctatt 311880tggatgcctt ttatttattt ctcttgcctg attgctctgg caaagaaata aggggatatt 311940ttgggtatag acacacatac aaggaaagta ccatgtgaag atgaaggcag atgtcaaggt 312000aatacaagag aagccaagga atgccgaaga ttgccagcaa accactagaa gctagaagag 312060agtcatggga tggattctcc ctcatagacc gtagaaggaa tggaccgtgc tgacaccttg 312120atctctgatt tctagacccc agaactctga gacaataaat tgctgttatt taagcaaatg 312180ttaacaattt ctagaagaaa tattgcagct atcaaattga aaaatctccc tacatgtggg 312240gcagaatttt aaaaaaaatc acccagtact atcaaagtag catggcaaaa catcaaaggt 312300aatgggaaaa atattaaaaa ttgatataaa ttttatgttt tgtgtattct gtcataattt 312360ttaaaaattg caaaaaatga tcagatagga aagatataag tgaacatgtg agtttaatct 312420tggggtgatt ttatagggtc tgagaaaggt gtggaaggaa gcaagagcaa aggggatctg 312480cttttcaaat attggcaata cctccatctc gacttcatct tcgttggatt ctggcagcac 312540agtagctggt gaagctcgtg tttggccaca gaatgcaagg gtaggactag aaaattaagg 312600gattgtcagg gatgtgtgtg tacacatgta gggaaaactc ctctttccat aattagtagt 312660tcctggatat gttggaaaga ataaagctca atatatcagt atatatccat tcagtctgat 312720atatatatat taaatacctc atctaagcca ggcactactt aggtttatac acacaaggat 312780aaataaaaca gagtttcttc cttcattcgt gggtaatttc actctgttgt actatttatt 312840acagttacag aaacaagata caatttttga aggtaatcta ttcagggtaa atattccatt 312900caaatggctt taaggatatt tgctggaacc tttcttgaat gtagaccttc ttttctagaa 312960ctttgctaca tgcctttgca ctccatcttg atgagatggt taagaaaaaa aaacataaat 313020aagcagatcc acgtgcttat catttaggta tcaatattat tgcctcaatt ttttctaaat 313080actttaagga ggcagataaa gatgatttat acactgaata tacttgagca tcggtgtaaa 313140aattcagtat taagacatta ctaaatttac tttcaacgtc caaactaaat gtgttttcaa 313200attaagtcga agcaactgca gagaagtcaa atatttgctc tcattaataa tacactggta 313260ctaaaaagaa gaataatgac actcttataa aggttttctt taacatctct aaaagattac 313320taatcatatt ttattcatcc ttaccacagt ttgtttttgt ggactacttt attagattta 313380tttcatggaa gtttttctca agtttcagca attggctaaa caatcaatac tttagctatc 313440aaagaaaact ttcctgagga ggaagaaatt tcttcttgaa tgaccttttt ttcaggagag 313500cacccatcac agatgaagaa ccaggtcact ctggaagtca agtctcacta aagcttgaag 313560cttctttttc tttctttctt tctttctttc tttctttctt tctttctttc tttctttctt 313620tctttttttt tagacagtct cactctgttg cctgggctgg aatgcagtgg tgcaatcttg 313680gctcactgca acctctgctt tctgggttca agcaattctt ttgcctcaga caccaagtag 313740ctgggattac aggtacatgc caccacgctt tggctaattt ttgtattttt agtagagatg 313800aggtttcacc atgttggcca ggctggcctt gaacacttaa cctcaggtga tctgccctcc 313860tcagcctccc aaaatgctga gattctagga gtgagccaca gctctggagc taaagcttct 313920taaatagaga gctgtgtcac acagaaaatg ccccatgggc agcaagtcat gtttctagga 313980ggctttatta ggtgataatt gcgcagatat cgatggcatg cttttttgtt gttgttagca 314040tccagaagaa aaaaaaaatc tacaagaggg gttgagcaaa gggacctgaa ttcgggaaaa 314100ttaagtgtaa gaatgcataa aatttaaagt aagttttact gaagcttttg tgctcagaga 314160gatatgtgtg gttagattcc ctctcaaagc caaacattat ctcctaatta tggccttaac 314220aaataacact agtatataac aacaatgaca agttaaaaaa aaaaacaaga cctagtttat 314280tttgagatct aaagtcaaaa actagtccta tttagtgcca ataggactag caatgttgtc 314340tacaatttgt gaagggaggt tctaataatg ctacttttat tttttaaaat ggtgatagaa 314400gaatgctaag catttcatgc ctataaacaa ggcactgaaa atgaaaggta aggtaagata 314460atgtcagcag ttaattaagg atggctttta atcttttctt tgatttttct tttcttttcc 314520tttagcctta aaaaattcca aactataaca aggagaggaa aaagccagca gaaggagagg 314580aagagggact cactatgatg gagtatcagc attggaagag tgggaagaac agacgggttg 314640gaaatcagaa gacatggcac ttgcttcctg ggcacagaga ttttggggaa ggatgcttac 314700tgtctttgta tacccatttt cttagctgta atatataaat aacaatatta gacagtgtga 314760tgaacaccct tgcaaactgt cacacactat acaaacagca gttattatcc acacacctag 314820agcccttgta aactgtcaca cgctatacag actgcagtta tcattatcca cacacctaga 314880gtgaacacgg tgaaaaacct ttgccttaag ggaagtgggt tagaacctct accacatcac 314940tgggccacat tacccagtca cagatgaaca actaggccac tctgcaggtt gtcccacaga 315000agcttctaaa atagagatgt gtcacacaga aaatgcccca ggagctgcaa gtcatgttgc 315060tgggaggctt tcttaggtga taattgggca aatatttttt gttactttta acagccagaa 315120aaaaatgtac aagagaggtt gagcaacaag atctgaaggc aggaaaatta agagtgcctt 315180gtgtgtaaag cctaaaaaga gaatgctgta gataagaaat tgagaaatac cacaaaatgc 315240ccacagggta caaggaacac tcccttttca cctcccatct gagctgcaat gcccatccga 315300gtgctgaata ctgactggaa ataaggcacg gtgatgcatc attctgttag atgtgcagcc 315360tccctgcttt tgtccacttc cctcacaaaa atactgattt caaacgccac ttgtcctctt 315420agtcttagta tctattttta actatacctg gagaagttct ctaattttga catctgaaat 315480catattttcc ccatattaat tctctctctc ttcttcccac aagaatataa gaaccaagaa 315540gccgtgaatt taaagaatgt atctctactg cctagaacag tgacatattg taggcattga 315600agaaatgtgt tgatgagtga ataaatgaag aaagtgatga aagacttagt tactaagcag 315660ctacaaataa tttacaaaaa ggaaatatta tgatcttatt atttccaaca tacatagatt 315720cagatgatat aaaacatttg ttttcccaaa aagtccctac taatgtctct tggtggatag 315780ttagcagtct ctttgaagtc tggtctccag tgtcttttct cccatttctt cctcctccca 315840cctttccttt ggtcttttat tcccttctat tcttccttgc ttcatcacct aatccacagt 315900ctcatttctg tcctggagag gagctccatc ttagatggta ggttttactt tccacctagg 315960agtagattca agtgttcaaa gtcttaaggg aggaacatct ataagaaaag caactcttgc 316020taaattaatt atacttccat agcagagttt ccatagcata ttatgctagt gaagtctcta 316080ttgctttcgt tttcctgtca tagatgagga agacaaggga aaaaagacaa ggctagaaac 316140tagtgcagaa aattccattg tgcaacaaga gctcacctta tggagatggc aactcaagaa 316200ctctacaaag ttttctaaaa ctgcttctca aagtttaatg cacacaacaa ttacctgaga 316260tcttgttagc aaattttgtt tcagtagttc tggggtgaag cttcaagttc tgcatttcta 316320ataaacaacc aggtggtcca tgggtccaca ccttgagtag tcaatctcta aaagaaaacg 316380tttcattttg gacctctact ttacagtcta ctgaaagtag tctactttgc attatggtct 316440actgaaaata cactgtgtaa gggaaagagc aagaggattt gggttcatct aggactgggt 316500ttgaaatctg gctccgctat tccttaagag acactgggca aggattattt ttaaaaatat 316560aaatttccta agcttcagta tcatctggaa tatgggtata ataatatctg tcttatagga 316620ttgctataaa aattaacttc ttttaaggaa ctttagcatg gggcaaagct tgatcccttc 316680ccctttcttt tcccatttca aattcttcaa aatggcccga ttcagcttca aattctgttt 316740caaattttgt ctatttctat tcttgaaaac tgaaagcata tacacccaga cagcttactg 316800tctatattaa cacaatttaa cttttcaacc tgtgcattaa cctgacttaa ttattccagg 316860aaatccttgc ccaggaagat ggagttgcaa ataactgctt atttcagaat cttcaaaatt 316920tatttatctg aaaaatcaat tcattctctt taagtaaata gcctcttctc gaaacaatag 316980attattaagc tgggcccaac aaatgattat ttactcttaa agactcttca gtgtactccc 317040tttactgtgt ccattaatta tacattatta tattataaca tctttcagta ccaatcagac 317100cacttcactg aaagacatgc cttaaagtag actctaaatc tttacaaatg ccagttttga 317160ttcttccctt gcaaaacacc actaagactc caccaaggta gtcagtggat tcccttacta 317220cagtaagaaa taaacttctc tttgcttgat caaacatgtg attttgctgg tgtcttgaga 317280gttggcagtt gacaatctct tctaataaaa ctatcatcca gacaaacata cttaattact 317340tatttcctgg aggtggcatg ctttttaata ttccatgctt ttgcacaatc aatttctttt 317400ttcctggaat atcatctttt atttaacctt caagactcat actaaatgtt agtctctctg 317460tgaaatattt attgaaaccc tttagcaata tagatttttt catgcttctg ttctcccata 317520gcattgagta ggccttttcc atcataccct attatttcct gattgcttgg ttacacattt 317580ttctttccca aacacaatgt gctctttaaa aatagggact atgccttatc cacctttgtc 317640accaatgtct taaagaattt taaaaaaaac tattagttga tcaataaata tttgttgaat 317700gttgaatgaa acacagttag cctggatatt tagaggaaga aaaatgggta aataaatatt 317760tgttaagaga aacttgagct tcttttcata aggatttcct ttgcatcttc ttaccctttt 317820tgttttaggt aatcctgaga aatgttacct ctgaagtcta ctacccctga aatagtagct 317880ctctgagcca gcaaaatagg acctttttga tgtctcaaat cctgtcaaaa aggtgatttc 317940tcatttcttt ctcatctagt aattattgat ggcaagtatg agcatgcagt tgggtcagtc 318000cttctatcag ggtagcagat aacttttatt cccccttcag ggaaatttta cctcctctgg 318060catcagtctg gatataaatg ttgctggttt catgatctga aattaagagt gaacattttg 318120tttagcccaa tcaaggctgg tagaaggaca aacttttata atgtgaaatt tatgaccctg 318180tgatggctga taaaaacaat ccctggcata gttatgcttc tatttaagtg ttaattatcc 318240cctatgcaca gtgtgtactc tttttgatag ccacaattca acacttaggc tattaaaggc 318300agatttatgc tatagcaata gaatattttt tatttccatc taaactgaaa tctagaagtt 318360ccataaaagt gtaagagctc atgtcatggc taacaggttt tgacaagagc taagttaata 318420tttgtagcag taaaccatct cctggttgac agtttgttga gacatcagag aagtctctga 318480ggagaaaaat taagtaaatc taaatggtac atttgaccaa ctataagcct ggactgacat 318540tttgctctag caaaagtaag ctgtttggaa aagagtgcaa aattatatgt atcatatctc 318600agtttctgat cattttagat catttgaggt ataaatattt aataaatatg tttaatgttc 318660agcagtatgt gaacactact ttcatatctc tctctttttg gctgtcatca aaaaatgaaa 318720gaaataacct tcattataac ataagctagt tagccctctg agtactccat gtaacattcg 318780cttcatacca taataacaat aagcattgta acatatttgt gtgaagtccc aatgatcttg 318840atatctggga ggcttgtgtt aatcagattt tttcaggaat gtaggtagca accataatat 318900ttcattgtaa gtttattgta atttttgcca ttatcatgct tgacgtgtgt gatccttaag 318960tactcctgtg aagtattaat gtacccatct tacagaggag aaggggactc aaacagaaga 319020cgtgcctttt cgtaggtcac acagaacatg gcagagctag agccagaact cagaactcct 319080gtctcccaga aacagtcccc tttgttctac aatacacaga tctctgttaa tatattttaa 319140aatgattata gaagagatgt tacagaccct tcacatagaa tttgatcaat gtttattttg 319200aggcaagtga actaagacag cattaatttt aattgcattc tccatttgag aacagtaatc 319260atgaaaagaa attaaaaggc tgagttcaag atcgattttc cagtctttgt agcaatccct 319320gaagcttcaa aaagcttgca gaaagacttt ttccagagac tgttttgact tcctcatatt 319380agactattaa cgaccatact tagtactcag tagaatatta ataatactgt gcttgaactg 319440agacaatttt tctgtgggta cttctacatt ttgaagagta tcaggacaaa tatgtgccca 319500tgcagggcat aacacaaatt gatgaagttg attggtagtt ttcaagcact agtatgtgcc 319560aggaactatg ctggacactt attttatata taatttttca attaatgttc ataacaatcc 319620tacatccatt tcatgggtat aaaaacagac tcagtggagt taaggaacct gtcaaaattc 319680acaagatgtt acataactga gtcaacattt gaacctgggt ctatccaatt tcaataattc 319740cacttcagta tgatgtttag ttcttttcaa actcacaaca aaggaaaaaa tctttgccta 319800gtcaggtggg gcctacattt aacccggata acctacattt ggaacgtggg agaaatgcaa 319860tttctgtttc cccgttctct cttccacatc tctttctaca cttgctggct actatttctc 319920actctttcac cccttccaga gactgaccag aagaaagaag ccattagaga aaaagaacac 319980cattgtacat gcccaacaca attgcaatcc agtataagag gctcccctgg atttgaatga 320040tgtccagagc tgacctgttc taccatgaga ctctttagtt gttctttgta gatctgtcca 320100gtgggggtct tttctatacc atcctagata cagaaaatgt ctccctgctg ccggccactt 320160accctgcccc accatcagcc cttaggtttt cagtggtcca gaagctaatg gatcttcttt 320220gactctccag ccagtcctct taaaagcttc tattcttgga gtcctctttt gatttccaag 320280aaacacatta gctcccagtt tgctctgcct ggtgaaaatg cagctcactc tcaatgcaac 320340catttttatc ctgttgtcag cagacacagc tgcagccaca gcctcttgtc tttcattttc 320400ctctcaggct tcagatgtta ttgaattggt tttgtagctt tcttcctttg acctattgtt 320460aaatacatta cataagtaga attcataata ctaatttttt tgtatatgag gaataaattc 320520tacctgtttt tcatgtagtt ttttttaaga atctatttgc taatatctcc ttttgtagct 320580ttgtgtctct gctcataatt agagaataat tgattagtac tattttttgg ttagattttg 320640ctaactttct aaaattatgt agaaatgatc ccatctgatt ccatgctctg gaaaatttct 320700ataaaatgga aggcaacatc tccttggata ttttaaacac tttctcataa aactcactat 320760gttaagtgtg atcaatagtc attaatagta taattcttag tgtttctctt tgtattatta 320820tatttaattt ctattgcttg atttatcagt ttttaaagat acatgtgatt cccagttatt 320880gcaacagatg aggcaacaat aacaataatg gagtgtaatc tattgttatg tactctgata 320940atgcaataat gtcaaactaa caagtaaatg gaagcagagg ggggagacaa aaggaaaagt 321000agagaaaata ggaattagcc aaacacacac aaaaaaaaac atgtccaaca tcattagtaa 321060tctgaaaagt acaaaacaaa ctcacaatga gatagtattt tatatgcacc aaattggaaa 321120atctgaaaat atctgataat attatgttag gccaggcaca gtggcatgca cctgtagttc 321180cagctgcttc agaggctaaa gcaagaaaat catttgagcc caggagttca aggctattgt 321240gcactatgat catgtgtatg agtagtcact gcactctagc ctggacaaca taatgagact 321300gtctctaaaa aaaattaaaa ataaaaataa aaatactcag tgttggcatg gatatgaaac 321360aactagacat ctatgtcttt gggtaaaaat gtaaattggt acgctttgta acaatactta 321420agtcttttta tggggatgta aatcaatata aaatgataac aacaacttat tttttaaatc 321480acaaatgcac tcattctatg acacagggat taagctccca caactattcc atagagaaac 321540ccttgcacat ataaatggat agaaatgcca tacaaaatac tcaaagcaac atactttgta 321600atatcacaaa actagtgata tagtttggat gtctgtctcc tccaaatcac acgttgaaat 321660gtgactccta atgttgaagg tggggcccag taggaggtgt ttgggtgatg ggagtggatc 321720cctcatgaat tgcttagtgc cctccctgca gtaatgtgtg agttttcact ctattcacaa 321780gagctggttg tttaaaatga gactggcacc tcctcctctc tctcgtgctt tctttcttgc 321840catgtgacac acctgctccc ccttcacctt ctgctatgat tggaagcttc ctgaggctct 321900tatcagaagc agattctggg gccatgtttc ttgtacagtc tgcagaacca tgagccaaat 321960aaacatattt tctttagaaa ttacccagcc tcaggtattc ctgtatagca atgcaaaatg 322020gactaatata actagaaaca actcaaatgt taattaatgg aagaaagact aaaaaatatt 322080gtatataaca ctatatatta t 322101 2 897 DNA Homo sapiens 2 atg aag gag acccgg ggc tac gga ggg gat gcc ccc ttc tgc acc cgc 48 ctc aac cac tcc tacaca ggc atg tgg gcg ccc gag cgt tcc gcc gag 96 gcg cgg ggc aac ctc acgcgc cct cca ggg tct ggc gag gat tgc gga 144 tcg gtg tcc gtg gcc ttc ccgatc acc atg ctg ctc act ggt ttc gtg 192 ggc aac gca ctg gcc atg ctg ctcgtg tcg cgc agc tac cgg cgc cgg 240 gag agc aag cgc aag aag tcc ttc ctgctg tgc atc ggc tgg ctg gcg 288 ctc acc gac ctg gtc ggg cag ctt ctc accacc ccg gtc gtc atc gtc 336 gtg tac ctg tcc aag cag cgt tgg gag cac atcgac ccg tcg ggg cgg 384 ctc tgc acc ttt ttc ggg ctg acc atg act gtt ttcggg ctc tcc tcg 432 ttg ttc atc gcc agc gcc atg gcc gtc gag cgg gcg ctggcc atc agg 480 gcg ccg cac tgg tat gcg agc cac atg aag acg cgt gcc acccgc gct 528 gtg ctg ctc ggc gtg tgg ctg gcc gtg ctc gcc ttc gcc ctg ctgccg 576 gtg ctg ggc gtg ggc cag tac acc gtc cag tgg ccc ggg acg tgg tgc624 ttc atc agc acc ggg cga ggg ggc aac ggg act agc tct tcg cat aac 672tgg ggc aac ctt ttc ttc gcc tct gcc ttt gcc ttc ctg ggg ctc ttg 720 gcgctg aca gtc acc ttt tcc tgc aac ctg gcc acc att aag gcc ctg 768 gtg tcccgc tgc cgg gcc aag gcc acg gca tct cag tcc agt gcc cag 816 tgg ggc cgcatc acg acc gag acg gcc att cag ctt atg ggg atc atg 864 tgc gtg ctg tcggtc tgc tgg tct ccg ctc ctg 897 3 180 DNA Homo sapiens 3 ata atg atg ttgaaa atg atc ttc aat cag aca tca gtt gag cac tgc 48 aag aca cac acg gagaag cag aaa gaa tgc aac ttc ttc tta ata gct 96 gtt cgc ctg gct tca ctgaac cag atc ttg gat cct tgg gtt tac ctg 144 ctg tta aga aag atc ctt cttcga aag ttt tgc cag 180 4 90 DNA Homo sapiens 4 gta gca aat gct gtc tccagc tgc tct aat gat gga cag aaa ggg cag 48 cct atc tca tta tct aat gaaata ata cag aca gaa gca tga 90 5 225 DNA Homo sapiens 5 aag tgt tcc ccagtc ttt gac tct tta aat tcc aat act gat tcc aaa 48 aca aat aaa tat tttgaa gac tca atg aat act ttc cat att ttg gcc 96 tat tta tat aag aaa gttaat aac att gac cct tca cag ctc ttc tgc 144 ctg ctc ctc aaa gtg gct ctatct aaa tat tta tta cta aaa tgt ttt 192 tcc tac agt cta cat gaa tac aaacct caa tag 225 6 147 DNA Homo sapiens 6 gat act cat gtg ggt ggg gca gagaac ata gaa aaa gat atg atg gaa 48 aac ctg tcc att ttc tac ctg tta accttc atc att ttg tgc cag gcc 96 ctg gaa gca aag aga gga agg gac cga ctgcat tta tct ttg aac act 144 tga 147 7 96 DNA Homo sapiens 7 atc agg taccac aca aac aac tat gca tcc agc tcc acc tcc tta ccc 48 tgc cag tgt tcctca acc ttg atg tgg agc gac cat ttg gaa agg tga 96 8 99 DNA Homo sapiens8 atc agg tac cac aca aac aac tat gca tcc agc tcc acc tcc tta ccc 48 tgccag tgt tcc tca acc ttg atg tgg agc gac cat ttg gaa aga taa 96 tga 99 927 DNA Homo sapiens 9 atg aga aaa aga aga ctc aga gag caa 27 10 78 DNAHomo sapiens 10 tta atc tgc agt ttg cga act ctc aga tac aga ggg caa ctgcac att 48 gtg ggc aag tac aaa cct atc gtg tgc tga 78 11 76 DNA Homosapiens 11 gag atg ggg cct gat gga agg tgt ttt tgt cat gca tgg agg caggtc 48 ccc agg act tgg tgc agt tct cat gat a 76 12 145 DNA Homo sapiens12 gct cct ctt ctt ccc acc tct act gtg att gat cct tca agg ttc tgt 48gct cag ccc ttc cgt tgg ttc ttg gat ttg tcc ttt ccc gcc atg tct 96 tcatca cat cca caa ctt cca cta aca ctt gcg agc ttc aaa ctt ctt 144 a 145 1332 DNA Homo sapiens 13 gag aac cct gca gtg tcc agc taa gct gat ga 32 1421 DNA Homo sapiens 14 gag gaa ttt tgg gga aat taa 21 15 1167 DNA Homosapiens CDS (1)...(1164) cDNA 15 atg aag gag acc cgg ggc tac gga ggg gatgcc ccc ttc tgc acc cgc 48 Met Lys Glu Thr Arg Gly Tyr Gly Gly Asp AlaPro Phe Cys Thr Arg 1 5 10 15 ctc aac cac tcc tac aca ggc atg tgg gcgccc gag cgt tcc gcc gag 96 Leu Asn His Ser Tyr Thr Gly Met Trp Ala ProGlu Arg Ser Ala Glu 20 25 30 gcg cgg ggc aac ctc acg cgc cct cca ggg tctggc gag gat tgc gga 144 Ala Arg Gly Asn Leu Thr Arg Pro Pro Gly Ser GlyGlu Asp Cys Gly 35 40 45 tcg gtg tcc gtg gcc ttc ccg atc acc atg ctg ctcact ggt ttc gtg 192 Ser Val Ser Val Ala Phe Pro Ile Thr Met Leu Leu ThrGly Phe Val 50 55 60 ggc aac gca ctg gcc atg ctg ctc gtg tcg cgc agc taccgg cgc cgg 240 Gly Asn Ala Leu Ala Met Leu Leu Val Ser Arg Ser Tyr ArgArg Arg 65 70 75 80 gag agc aag cgc aag aag tcc ttc ctg ctg tgc atc ggctgg ctg gcg 288 Glu Ser Lys Arg Lys Lys Ser Phe Leu Leu Cys Ile Gly TrpLeu Ala 85 90 95 ctc acc gac ctg gtc ggg cag ctt ctc acc acc ccg gtc gtcatc gtc 336 Leu Thr Asp Leu Val Gly Gln Leu Leu Thr Thr Pro Val Val IleVal 100 105 110 gtg tac ctg tcc aag cag cgt tgg gag cac atc gac ccg tcgggg cgg 384 Val Tyr Leu Ser Lys Gln Arg Trp Glu His Ile Asp Pro Ser GlyArg 115 120 125 ctc tgc acc ttt ttc ggg ctg acc atg act gtt ttc ggg ctctcc tcg 432 Leu Cys Thr Phe Phe Gly Leu Thr Met Thr Val Phe Gly Leu SerSer 130 135 140 ttg ttc atc gcc agc gcc atg gcc gtc gag cgg gcg ctg gccatc agg 480 Leu Phe Ile Ala Ser Ala Met Ala Val Glu Arg Ala Leu Ala IleArg 145 150 155 160 gcg ccg cac tgg tat gcg agc cac atg aag acg cgt gccacc cgc gct 528 Ala Pro His Trp Tyr Ala Ser His Met Lys Thr Arg Ala ThrArg Ala 165 170 175 gtg ctg ctc ggc gtg tgg ctg gcc gtg ctc gcc ttc gccctg ctg ccg 576 Val Leu Leu Gly Val Trp Leu Ala Val Leu Ala Phe Ala LeuLeu Pro 180 185 190 gtg ctg ggc gtg ggc cag tac acc gtc cag tgg ccc gggacg tgg tgc 624 Val Leu Gly Val Gly Gln Tyr Thr Val Gln Trp Pro Gly ThrTrp Cys 195 200 205 ttc atc agc acc ggg cga ggg ggc aac ggg act agc tcttcg cat aac 672 Phe Ile Ser Thr Gly Arg Gly Gly Asn Gly Thr Ser Ser SerHis Asn 210 215 220 tgg ggc aac ctt ttc ttc gcc tct gcc ttt gcc ttc ctgggg ctc ttg 720 Trp Gly Asn Leu Phe Phe Ala Ser Ala Phe Ala Phe Leu GlyLeu Leu 225 230 235 240 gcg ctg aca gtc acc ttt tcc tgc aac ctg gcc accatt aag gcc ctg 768 Ala Leu Thr Val Thr Phe Ser Cys Asn Leu Ala Thr IleLys Ala Leu 245 250 255 gtg tcc cgc tgc cgg gcc aag gcc acg gca tct cagtcc agt gcc cag 816 Val Ser Arg Cys Arg Ala Lys Ala Thr Ala Ser Gln SerSer Ala Gln 260 265 270 tgg ggc cgc atc acg acc gag acg gcc att cag cttatg ggg atc atg 864 Trp Gly Arg Ile Thr Thr Glu Thr Ala Ile Gln Leu MetGly Ile Met 275 280 285 tgc gtg ctg tcg gtc tgc tgg tct ccg ctc ctg ataatg atg ttg aaa 912 Cys Val Leu Ser Val Cys Trp Ser Pro Leu Leu Ile MetMet Leu Lys 290 295 300 atg atc ttc aat cag aca tca gtt gag cac tgc aagaca cac acg gag 960 Met Ile Phe Asn Gln Thr Ser Val Glu His Cys Lys ThrHis Thr Glu 305 310 315 320 aag cag aaa gaa tgc aac ttc ttc tta ata gctgtt cgc ctg gct tca 1008 Lys Gln Lys Glu Cys Asn Phe Phe Leu Ile Ala ValArg Leu Ala Ser 325 330 335 ctg aac cag atc ttg gat cct tgg gtt tac ctgctg tta aga aag atc 1056 Leu Asn Gln Ile Leu Asp Pro Trp Val Tyr Leu LeuLeu Arg Lys Ile 340 345 350 ctt ctt cga aag ttt tgc cag gta gca aat gctgtc tcc agc tgc tct 1104 Leu Leu Arg Lys Phe Cys Gln Val Ala Asn Ala ValSer Ser Cys Ser 355 360 365 aat gat gga cag aaa ggg cag cct atc tca ttatct aat gaa ata ata 1152 Asn Asp Gly Gln Lys Gly Gln Pro Ile Ser Leu SerAsn Glu Ile Ile 370 375 380 cag aca gaa gca tga 1167 Gln Thr Glu Ala 38516 388 PRT Homo sapiens 16 Met Lys Glu Thr Arg Gly Tyr Gly Gly Asp AlaPro Phe Cys Thr Arg 1 5 10 15 Leu Asn His Ser Tyr Thr Gly Met Trp AlaPro Glu Arg Ser Ala Glu 20 25 30 Ala Arg Gly Asn Leu Thr Arg Pro Pro GlySer Gly Glu Asp Cys Gly 35 40 45 Ser Val Ser Val Ala Phe Pro Ile Thr MetLeu Leu Thr Gly Phe Val 50 55 60 Gly Asn Ala Leu Ala Met Leu Leu Val SerArg Ser Tyr Arg Arg Arg 65 70 75 80 Glu Ser Lys Arg Lys Lys Ser Phe LeuLeu Cys Ile Gly Trp Leu Ala 85 90 95 Leu Thr Asp Leu Val Gly Gln Leu LeuThr Thr Pro Val Val Ile Val 100 105 110 Val Tyr Leu Ser Lys Gln Arg TrpGlu His Ile Asp Pro Ser Gly Arg 115 120 125 Leu Cys Thr Phe Phe Gly LeuThr Met Thr Val Phe Gly Leu Ser Ser 130 135 140 Leu Phe Ile Ala Ser AlaMet Ala Val Glu Arg Ala Leu Ala Ile Arg 145 150 155 160 Ala Pro His TrpTyr Ala Ser His Met Lys Thr Arg Ala Thr Arg Ala 165 170 175 Val Leu LeuGly Val Trp Leu Ala Val Leu Ala Phe Ala Leu Leu Pro 180 185 190 Val LeuGly Val Gly Gln Tyr Thr Val Gln Trp Pro Gly Thr Trp Cys 195 200 205 PheIle Ser Thr Gly Arg Gly Gly Asn Gly Thr Ser Ser Ser His Asn 210 215 220Trp Gly Asn Leu Phe Phe Ala Ser Ala Phe Ala Phe Leu Gly Leu Leu 225 230235 240 Ala Leu Thr Val Thr Phe Ser Cys Asn Leu Ala Thr Ile Lys Ala Leu245 250 255 Val Ser Arg Cys Arg Ala Lys Ala Thr Ala Ser Gln Ser Ser AlaGln 260 265 270 Trp Gly Arg Ile Thr Thr Glu Thr Ala Ile Gln Leu Met GlyIle Met 275 280 285 Cys Val Leu Ser Val Cys Trp Ser Pro Leu Leu Ile MetMet Leu Lys 290 295 300 Met Ile Phe Asn Gln Thr Ser Val Glu His Cys LysThr His Thr Glu 305 310 315 320 Lys Gln Lys Glu Cys Asn Phe Phe Leu IleAla Val Arg Leu Ala Ser 325 330 335 Leu Asn Gln Ile Leu Asp Pro Trp ValTyr Leu Leu Leu Arg Lys Ile 340 345 350 Leu Leu Arg Lys Phe Cys Gln ValAla Asn Ala Val Ser Ser Cys Ser 355 360 365 Asn Asp Gly Gln Lys Gly GlnPro Ile Ser Leu Ser Asn Glu Ile Ile 370 375 380 Gln Thr Glu Ala 385 171224 DNA Homo sapiens CDS (1)...(1221) cDNA 17 atg aag gag acc cgg ggctac gga ggg gat gcc ccc ttc tgc acc cgc 48 Met Lys Glu Thr Arg Gly TyrGly Gly Asp Ala Pro Phe Cys Thr Arg 1 5 10 15 ctc aac cac tcc tac acaggc atg tgg gcg ccc gag cgt tcc gcc gag 96 Leu Asn His Ser Tyr Thr GlyMet Trp Ala Pro Glu Arg Ser Ala Glu 20 25 30 gcg cgg ggc aac ctc acg cgccct cca ggg tct ggc gag gat tgc gga 144 Ala Arg Gly Asn Leu Thr Arg ProPro Gly Ser Gly Glu Asp Cys Gly 35 40 45 tcg gtg tcc gtg gcc ttc ccg atcacc atg ctg ctc act ggt ttc gtg 192 Ser Val Ser Val Ala Phe Pro Ile ThrMet Leu Leu Thr Gly Phe Val 50 55 60 ggc aac gca ctg gcc atg ctg ctc gtgtcg cgc agc tac cgg cgc cgg 240 Gly Asn Ala Leu Ala Met Leu Leu Val SerArg Ser Tyr Arg Arg Arg 65 70 75 80 gag agc aag cgc aag aag tcc ttc ctgctg tgc atc ggc tgg ctg gcg 288 Glu Ser Lys Arg Lys Lys Ser Phe Leu LeuCys Ile Gly Trp Leu Ala 85 90 95 ctc acc gac ctg gtc ggg cag ctt ctc accacc ccg gtc gtc atc gtc 336 Leu Thr Asp Leu Val Gly Gln Leu Leu Thr ThrPro Val Val Ile Val 100 105 110 gtg tac ctg tcc aag cag cgt tgg gag cacatc gac ccg tcg ggg cgg 384 Val Tyr Leu Ser Lys Gln Arg Trp Glu His IleAsp Pro Ser Gly Arg 115 120 125 ctc tgc acc ttt ttc ggg ctg acc atg actgtt ttc ggg ctc tcc tcg 432 Leu Cys Thr Phe Phe Gly Leu Thr Met Thr ValPhe Gly Leu Ser Ser 130 135 140 ttg ttc atc gcc agc gcc atg gcc gtc gagcgg gcg ctg gcc atc agg 480 Leu Phe Ile Ala Ser Ala Met Ala Val Glu ArgAla Leu Ala Ile Arg 145 150 155 160 gcg ccg cac tgg tat gcg agc cac atgaag acg cgt gcc acc cgc gct 528 Ala Pro His Trp Tyr Ala Ser His Met LysThr Arg Ala Thr Arg Ala 165 170 175 gtg ctg ctc ggc gtg tgg ctg gcc gtgctc gcc ttc gcc ctg ctg ccg 576 Val Leu Leu Gly Val Trp Leu Ala Val LeuAla Phe Ala Leu Leu Pro 180 185 190 gtg ctg ggc gtg ggc cag tac acc gtccag tgg ccc ggg acg tgg tgc 624 Val Leu Gly Val Gly Gln Tyr Thr Val GlnTrp Pro Gly Thr Trp Cys 195 200 205 ttc atc agc acc ggg cga ggg ggc aacggg act agc tct tcg cat aac 672 Phe Ile Ser Thr Gly Arg Gly Gly Asn GlyThr Ser Ser Ser His Asn 210 215 220 tgg ggc aac ctt ttc ttc gcc tct gccttt gcc ttc ctg ggg ctc ttg 720 Trp Gly Asn Leu Phe Phe Ala Ser Ala PheAla Phe Leu Gly Leu Leu 225 230 235 240 gcg ctg aca gtc acc ttt tcc tgcaac ctg gcc acc att aag gcc ctg 768 Ala Leu Thr Val Thr Phe Ser Cys AsnLeu Ala Thr Ile Lys Ala Leu 245 250 255 gtg tcc cgc tgc cgg gcc aag gccacg gca tct cag tcc agt gcc cag 816 Val Ser Arg Cys Arg Ala Lys Ala ThrAla Ser Gln Ser Ser Ala Gln 260 265 270 tgg ggc cgc atc acg acc gag acggcc att cag ctt atg ggg atc atg 864 Trp Gly Arg Ile Thr Thr Glu Thr AlaIle Gln Leu Met Gly Ile Met 275 280 285 tgc gtg ctg tcg gtc tgc tgg tctccg ctc ctg ata atg atg ttg aaa 912 Cys Val Leu Ser Val Cys Trp Ser ProLeu Leu Ile Met Met Leu Lys 290 295 300 atg atc ttc aat cag aca tca gttgag cac tgc aag aca cac acg gag 960 Met Ile Phe Asn Gln Thr Ser Val GluHis Cys Lys Thr His Thr Glu 305 310 315 320 aag cag aaa gaa tgc aac ttcttc tta ata gct gtt cgc ctg gct tca 1008 Lys Gln Lys Glu Cys Asn Phe PheLeu Ile Ala Val Arg Leu Ala Ser 325 330 335 ctg aac cag atc ttg gat ccttgg gtt tac ctg ctg tta aga aag atc 1056 Leu Asn Gln Ile Leu Asp Pro TrpVal Tyr Leu Leu Leu Arg Lys Ile 340 345 350 ctt ctt cga aag ttt tgc caggat act cat gtg ggt ggg gca gag aac 1104 Leu Leu Arg Lys Phe Cys Gln AspThr His Val Gly Gly Ala Glu Asn 355 360 365 ata gaa aaa gat atg atg gaaaac ctg tcc att ttc tac ctg tta acc 1152 Ile Glu Lys Asp Met Met Glu AsnLeu Ser Ile Phe Tyr Leu Leu Thr 370 375 380 ttc atc att ttg tgc cag gccctg gaa gca aag aga gga agg gac cga 1200 Phe Ile Ile Leu Cys Gln Ala LeuGlu Ala Lys Arg Gly Arg Asp Arg 385 390 395 400 ctg cat tta tct ttg aacact tga 1224 Leu His Leu Ser Leu Asn Thr 405 18 407 PRT Homo sapiens 18Met Lys Glu Thr Arg Gly Tyr Gly Gly Asp Ala Pro Phe Cys Thr Arg 1 5 1015 Leu Asn His Ser Tyr Thr Gly Met Trp Ala Pro Glu Arg Ser Ala Glu 20 2530 Ala Arg Gly Asn Leu Thr Arg Pro Pro Gly Ser Gly Glu Asp Cys Gly 35 4045 Ser Val Ser Val Ala Phe Pro Ile Thr Met Leu Leu Thr Gly Phe Val 50 5560 Gly Asn Ala Leu Ala Met Leu Leu Val Ser Arg Ser Tyr Arg Arg Arg 65 7075 80 Glu Ser Lys Arg Lys Lys Ser Phe Leu Leu Cys Ile Gly Trp Leu Ala 8590 95 Leu Thr Asp Leu Val Gly Gln Leu Leu Thr Thr Pro Val Val Ile Val100 105 110 Val Tyr Leu Ser Lys Gln Arg Trp Glu His Ile Asp Pro Ser GlyArg 115 120 125 Leu Cys Thr Phe Phe Gly Leu Thr Met Thr Val Phe Gly LeuSer Ser 130 135 140 Leu Phe Ile Ala Ser Ala Met Ala Val Glu Arg Ala LeuAla Ile Arg 145 150 155 160 Ala Pro His Trp Tyr Ala Ser His Met Lys ThrArg Ala Thr Arg Ala 165 170 175 Val Leu Leu Gly Val Trp Leu Ala Val LeuAla Phe Ala Leu Leu Pro 180 185 190 Val Leu Gly Val Gly Gln Tyr Thr ValGln Trp Pro Gly Thr Trp Cys 195 200 205 Phe Ile Ser Thr Gly Arg Gly GlyAsn Gly Thr Ser Ser Ser His Asn 210 215 220 Trp Gly Asn Leu Phe Phe AlaSer Ala Phe Ala Phe Leu Gly Leu Leu 225 230 235 240 Ala Leu Thr Val ThrPhe Ser Cys Asn Leu Ala Thr Ile Lys Ala Leu 245 250 255 Val Ser Arg CysArg Ala Lys Ala Thr Ala Ser Gln Ser Ser Ala Gln 260 265 270 Trp Gly ArgIle Thr Thr Glu Thr Ala Ile Gln Leu Met Gly Ile Met 275 280 285 Cys ValLeu Ser Val Cys Trp Ser Pro Leu Leu Ile Met Met Leu Lys 290 295 300 MetIle Phe Asn Gln Thr Ser Val Glu His Cys Lys Thr His Thr Glu 305 310 315320 Lys Gln Lys Glu Cys Asn Phe Phe Leu Ile Ala Val Arg Leu Ala Ser 325330 335 Leu Asn Gln Ile Leu Asp Pro Trp Val Tyr Leu Leu Leu Arg Lys Ile340 345 350 Leu Leu Arg Lys Phe Cys Gln Asp Thr His Val Gly Gly Ala GluAsn 355 360 365 Ile Glu Lys Asp Met Met Glu Asn Leu Ser Ile Phe Tyr LeuLeu Thr 370 375 380 Phe Ile Ile Leu Cys Gln Ala Leu Glu Ala Lys Arg GlyArg Asp Arg 385 390 395 400 Leu His Leu Ser Leu Asn Thr 405 19 1302 DNAHomo sapiens CDS (1)...(1299) cDNA 19 atg aag gag acc cgg ggc tac ggaggg gat gcc ccc ttc tgc acc cgc 48 Met Lys Glu Thr Arg Gly Tyr Gly GlyAsp Ala Pro Phe Cys Thr Arg 1 5 10 15 ctc aac cac tcc tac aca ggc atgtgg gcg ccc gag cgt tcc gcc gag 96 Leu Asn His Ser Tyr Thr Gly Met TrpAla Pro Glu Arg Ser Ala Glu 20 25 30 gcg cgg ggc aac ctc acg cgc cct ccaggg tct ggc gag gat tgc gga 144 Ala Arg Gly Asn Leu Thr Arg Pro Pro GlySer Gly Glu Asp Cys Gly 35 40 45 tcg gtg tcc gtg gcc ttc ccg atc acc atgctg ctc act ggt ttc gtg 192 Ser Val Ser Val Ala Phe Pro Ile Thr Met LeuLeu Thr Gly Phe Val 50 55 60 ggc aac gca ctg gcc atg ctg ctc gtg tcg cgcagc tac cgg cgc cgg 240 Gly Asn Ala Leu Ala Met Leu Leu Val Ser Arg SerTyr Arg Arg Arg 65 70 75 80 gag agc aag cgc aag aag tcc ttc ctg ctg tgcatc ggc tgg ctg gcg 288 Glu Ser Lys Arg Lys Lys Ser Phe Leu Leu Cys IleGly Trp Leu Ala 85 90 95 ctc acc gac ctg gtc ggg cag ctt ctc acc acc ccggtc gtc atc gtc 336 Leu Thr Asp Leu Val Gly Gln Leu Leu Thr Thr Pro ValVal Ile Val 100 105 110 gtg tac ctg tcc aag cag cgt tgg gag cac atc gacccg tcg ggg cgg 384 Val Tyr Leu Ser Lys Gln Arg Trp Glu His Ile Asp ProSer Gly Arg 115 120 125 ctc tgc acc ttt ttc ggg ctg acc atg act gtt ttcggg ctc tcc tcg 432 Leu Cys Thr Phe Phe Gly Leu Thr Met Thr Val Phe GlyLeu Ser Ser 130 135 140 ttg ttc atc gcc agc gcc atg gcc gtc gag cgg gcgctg gcc atc agg 480 Leu Phe Ile Ala Ser Ala Met Ala Val Glu Arg Ala LeuAla Ile Arg 145 150 155 160 gcg ccg cac tgg tat gcg agc cac atg aag acgcgt gcc acc cgc gct 528 Ala Pro His Trp Tyr Ala Ser His Met Lys Thr ArgAla Thr Arg Ala 165 170 175 gtg ctg ctc ggc gtg tgg ctg gcc gtg ctc gccttc gcc ctg ctg ccg 576 Val Leu Leu Gly Val Trp Leu Ala Val Leu Ala PheAla Leu Leu Pro 180 185 190 gtg ctg ggc gtg ggc cag tac acc gtc cag tggccc ggg acg tgg tgc 624 Val Leu Gly Val Gly Gln Tyr Thr Val Gln Trp ProGly Thr Trp Cys 195 200 205 ttc atc agc acc ggg cga ggg ggc aac ggg actagc tct tcg cat aac 672 Phe Ile Ser Thr Gly Arg Gly Gly Asn Gly Thr SerSer Ser His Asn 210 215 220 tgg ggc aac ctt ttc ttc gcc tct gcc ttt gccttc ctg ggg ctc ttg 720 Trp Gly Asn Leu Phe Phe Ala Ser Ala Phe Ala PheLeu Gly Leu Leu 225 230 235 240 gcg ctg aca gtc acc ttt tcc tgc aac ctggcc acc att aag gcc ctg 768 Ala Leu Thr Val Thr Phe Ser Cys Asn Leu AlaThr Ile Lys Ala Leu 245 250 255 gtg tcc cgc tgc cgg gcc aag gcc acg gcatct cag tcc agt gcc cag 816 Val Ser Arg Cys Arg Ala Lys Ala Thr Ala SerGln Ser Ser Ala Gln 260 265 270 tgg ggc cgc atc acg acc gag acg gcc attcag ctt atg ggg atc atg 864 Trp Gly Arg Ile Thr Thr Glu Thr Ala Ile GlnLeu Met Gly Ile Met 275 280 285 tgc gtg ctg tcg gtc tgc tgg tct ccg ctcctg ata atg atg ttg aaa 912 Cys Val Leu Ser Val Cys Trp Ser Pro Leu LeuIle Met Met Leu Lys 290 295 300 atg atc ttc aat cag aca tca gtt gag cactgc aag aca cac acg gag 960 Met Ile Phe Asn Gln Thr Ser Val Glu His CysLys Thr His Thr Glu 305 310 315 320 aag cag aaa gaa tgc aac ttc ttc ttaata gct gtt cgc ctg gct tca 1008 Lys Gln Lys Glu Cys Asn Phe Phe Leu IleAla Val Arg Leu Ala Ser 325 330 335 ctg aac cag atc ttg gat cct tgg gtttac ctg ctg tta aga aag atc 1056 Leu Asn Gln Ile Leu Asp Pro Trp Val TyrLeu Leu Leu Arg Lys Ile 340 345 350 ctt ctt cga aag ttt tgc cag aag tgttcc cca gtc ttt gac tct tta 1104 Leu Leu Arg Lys Phe Cys Gln Lys Cys SerPro Val Phe Asp Ser Leu 355 360 365 aat tcc aat act gat tcc aaa aca aataaa tat ttt gaa gac tca atg 1152 Asn Ser Asn Thr Asp Ser Lys Thr Asn LysTyr Phe Glu Asp Ser Met 370 375 380 aat act ttc cat att ttg gcc tat ttatat aag aaa gtt aat aac att 1200 Asn Thr Phe His Ile Leu Ala Tyr Leu TyrLys Lys Val Asn Asn Ile 385 390 395 400 gac cct tca cag ctc ttc tgc ctgctc ctc aaa gtg gct cta tct aaa 1248 Asp Pro Ser Gln Leu Phe Cys Leu LeuLeu Lys Val Ala Leu Ser Lys 405 410 415 tat tta tta cta aaa tgt ttt tcctac agt cta cat gaa tac aaa cct 1296 Tyr Leu Leu Leu Lys Cys Phe Ser TyrSer Leu His Glu Tyr Lys Pro 420 425 430 caa tag 1302 Gln 20 433 PRT Homosapiens 20 Met Lys Glu Thr Arg Gly Tyr Gly Gly Asp Ala Pro Phe Cys ThrArg 1 5 10 15 Leu Asn His Ser Tyr Thr Gly Met Trp Ala Pro Glu Arg SerAla Glu 20 25 30 Ala Arg Gly Asn Leu Thr Arg Pro Pro Gly Ser Gly Glu AspCys Gly 35 40 45 Ser Val Ser Val Ala Phe Pro Ile Thr Met Leu Leu Thr GlyPhe Val 50 55 60 Gly Asn Ala Leu Ala Met Leu Leu Val Ser Arg Ser Tyr ArgArg Arg 65 70 75 80 Glu Ser Lys Arg Lys Lys Ser Phe Leu Leu Cys Ile GlyTrp Leu Ala 85 90 95 Leu Thr Asp Leu Val Gly Gln Leu Leu Thr Thr Pro ValVal Ile Val 100 105 110 Val Tyr Leu Ser Lys Gln Arg Trp Glu His Ile AspPro Ser Gly Arg 115 120 125 Leu Cys Thr Phe Phe Gly Leu Thr Met Thr ValPhe Gly Leu Ser Ser 130 135 140 Leu Phe Ile Ala Ser Ala Met Ala Val GluArg Ala Leu Ala Ile Arg 145 150 155 160 Ala Pro His Trp Tyr Ala Ser HisMet Lys Thr Arg Ala Thr Arg Ala 165 170 175 Val Leu Leu Gly Val Trp LeuAla Val Leu Ala Phe Ala Leu Leu Pro 180 185 190 Val Leu Gly Val Gly GlnTyr Thr Val Gln Trp Pro Gly Thr Trp Cys 195 200 205 Phe Ile Ser Thr GlyArg Gly Gly Asn Gly Thr Ser Ser Ser His Asn 210 215 220 Trp Gly Asn LeuPhe Phe Ala Ser Ala Phe Ala Phe Leu Gly Leu Leu 225 230 235 240 Ala LeuThr Val Thr Phe Ser Cys Asn Leu Ala Thr Ile Lys Ala Leu 245 250 255 ValSer Arg Cys Arg Ala Lys Ala Thr Ala Ser Gln Ser Ser Ala Gln 260 265 270Trp Gly Arg Ile Thr Thr Glu Thr Ala Ile Gln Leu Met Gly Ile Met 275 280285 Cys Val Leu Ser Val Cys Trp Ser Pro Leu Leu Ile Met Met Leu Lys 290295 300 Met Ile Phe Asn Gln Thr Ser Val Glu His Cys Lys Thr His Thr Glu305 310 315 320 Lys Gln Lys Glu Cys Asn Phe Phe Leu Ile Ala Val Arg LeuAla Ser 325 330 335 Leu Asn Gln Ile Leu Asp Pro Trp Val Tyr Leu Leu LeuArg Lys Ile 340 345 350 Leu Leu Arg Lys Phe Cys Gln Lys Cys Ser Pro ValPhe Asp Ser Leu 355 360 365 Asn Ser Asn Thr Asp Ser Lys Thr Asn Lys TyrPhe Glu Asp Ser Met 370 375 380 Asn Thr Phe His Ile Leu Ala Tyr Leu TyrLys Lys Val Asn Asn Ile 385 390 395 400 Asp Pro Ser Gln Leu Phe Cys LeuLeu Leu Lys Val Ala Leu Ser Lys 405 410 415 Tyr Leu Leu Leu Lys Cys PheSer Tyr Ser Leu His Glu Tyr Lys Pro 420 425 430 Gln 21 1173 DNA Homosapiens CDS (1)...(1170) cDNA 21 atg aag gag acc cgg ggc tac gga ggg gatgcc ccc ttc tgc acc cgc 48 Met Lys Glu Thr Arg Gly Tyr Gly Gly Asp AlaPro Phe Cys Thr Arg 1 5 10 15 ctc aac cac tcc tac aca ggc atg tgg gcgccc gag cgt tcc gcc gag 96 Leu Asn His Ser Tyr Thr Gly Met Trp Ala ProGlu Arg Ser Ala Glu 20 25 30 gcg cgg ggc aac ctc acg cgc cct cca ggg tctggc gag gat tgc gga 144 Ala Arg Gly Asn Leu Thr Arg Pro Pro Gly Ser GlyGlu Asp Cys Gly 35 40 45 tcg gtg tcc gtg gcc ttc ccg atc acc atg ctg ctcact ggt ttc gtg 192 Ser Val Ser Val Ala Phe Pro Ile Thr Met Leu Leu ThrGly Phe Val 50 55 60 ggc aac gca ctg gcc atg ctg ctc gtg tcg cgc agc taccgg cgc cgg 240 Gly Asn Ala Leu Ala Met Leu Leu Val Ser Arg Ser Tyr ArgArg Arg 65 70 75 80 gag agc aag cgc aag aag tcc ttc ctg ctg tgc atc ggctgg ctg gcg 288 Glu Ser Lys Arg Lys Lys Ser Phe Leu Leu Cys Ile Gly TrpLeu Ala 85 90 95 ctc acc gac ctg gtc ggg cag ctt ctc acc acc ccg gtc gtcatc gtc 336 Leu Thr Asp Leu Val Gly Gln Leu Leu Thr Thr Pro Val Val IleVal 100 105 110 gtg tac ctg tcc aag cag cgt tgg gag cac atc gac ccg tcgggg cgg 384 Val Tyr Leu Ser Lys Gln Arg Trp Glu His Ile Asp Pro Ser GlyArg 115 120 125 ctc tgc acc ttt ttc ggg ctg acc atg act gtt ttc ggg ctctcc tcg 432 Leu Cys Thr Phe Phe Gly Leu Thr Met Thr Val Phe Gly Leu SerSer 130 135 140 ttg ttc atc gcc agc gcc atg gcc gtc gag cgg gcg ctg gccatc agg 480 Leu Phe Ile Ala Ser Ala Met Ala Val Glu Arg Ala Leu Ala IleArg 145 150 155 160 gcg ccg cac tgg tat gcg agc cac atg aag acg cgt gccacc cgc gct 528 Ala Pro His Trp Tyr Ala Ser His Met Lys Thr Arg Ala ThrArg Ala 165 170 175 gtg ctg ctc ggc gtg tgg ctg gcc gtg ctc gcc ttc gccctg ctg ccg 576 Val Leu Leu Gly Val Trp Leu Ala Val Leu Ala Phe Ala LeuLeu Pro 180 185 190 gtg ctg ggc gtg ggc cag tac acc gtc cag tgg ccc gggacg tgg tgc 624 Val Leu Gly Val Gly Gln Tyr Thr Val Gln Trp Pro Gly ThrTrp Cys 195 200 205 ttc atc agc acc ggg cga ggg ggc aac ggg act agc tcttcg cat aac 672 Phe Ile Ser Thr Gly Arg Gly Gly Asn Gly Thr Ser Ser SerHis Asn 210 215 220 tgg ggc aac ctt ttc ttc gcc tct gcc ttt gcc ttc ctgggg ctc ttg 720 Trp Gly Asn Leu Phe Phe Ala Ser Ala Phe Ala Phe Leu GlyLeu Leu 225 230 235 240 gcg ctg aca gtc acc ttt tcc tgc aac ctg gcc accatt aag gcc ctg 768 Ala Leu Thr Val Thr Phe Ser Cys Asn Leu Ala Thr IleLys Ala Leu 245 250 255 gtg tcc cgc tgc cgg gcc aag gcc acg gca tct cagtcc agt gcc cag 816 Val Ser Arg Cys Arg Ala Lys Ala Thr Ala Ser Gln SerSer Ala Gln 260 265 270 tgg ggc cgc atc acg acc gag acg gcc att cag cttatg ggg atc atg 864 Trp Gly Arg Ile Thr Thr Glu Thr Ala Ile Gln Leu MetGly Ile Met 275 280 285 tgc gtg ctg tcg gtc tgc tgg tct ccg ctc ctg ataatg atg ttg aaa 912 Cys Val Leu Ser Val Cys Trp Ser Pro Leu Leu Ile MetMet Leu Lys 290 295 300 atg atc ttc aat cag aca tca gtt gag cac tgc aagaca cac acg gag 960 Met Ile Phe Asn Gln Thr Ser Val Glu His Cys Lys ThrHis Thr Glu 305 310 315 320 aag cag aaa gaa tgc aac ttc ttc tta ata gctgtt cgc ctg gct tca 1008 Lys Gln Lys Glu Cys Asn Phe Phe Leu Ile Ala ValArg Leu Ala Ser 325 330 335 ctg aac cag atc ttg gat cct tgg gtt tac ctgctg tta aga aag atc 1056 Leu Asn Gln Ile Leu Asp Pro Trp Val Tyr Leu LeuLeu Arg Lys Ile 340 345 350 ctt ctt cga aag ttt tgc cag atc agg tac cacaca aac aac tat gca 1104 Leu Leu Arg Lys Phe Cys Gln Ile Arg Tyr His ThrAsn Asn Tyr Ala 355 360 365 tcc agc tcc acc tcc tta ccc tgc cag tgt tcctca acc ttg atg tgg 1152 Ser Ser Ser Thr Ser Leu Pro Cys Gln Cys Ser SerThr Leu Met Trp 370 375 380 agc gac cat ttg gaa agg tga 1173 Ser Asp HisLeu Glu Arg 385 390 22 390 PRT Homo sapiens 22 Met Lys Glu Thr Arg GlyTyr Gly Gly Asp Ala Pro Phe Cys Thr Arg 1 5 10 15 Leu Asn His Ser TyrThr Gly Met Trp Ala Pro Glu Arg Ser Ala Glu 20 25 30 Ala Arg Gly Asn LeuThr Arg Pro Pro Gly Ser Gly Glu Asp Cys Gly 35 40 45 Ser Val Ser Val AlaPhe Pro Ile Thr Met Leu Leu Thr Gly Phe Val 50 55 60 Gly Asn Ala Leu AlaMet Leu Leu Val Ser Arg Ser Tyr Arg Arg Arg 65 70 75 80 Glu Ser Lys ArgLys Lys Ser Phe Leu Leu Cys Ile Gly Trp Leu Ala 85 90 95 Leu Thr Asp LeuVal Gly Gln Leu Leu Thr Thr Pro Val Val Ile Val 100 105 110 Val Tyr LeuSer Lys Gln Arg Trp Glu His Ile Asp Pro Ser Gly Arg 115 120 125 Leu CysThr Phe Phe Gly Leu Thr Met Thr Val Phe Gly Leu Ser Ser 130 135 140 LeuPhe Ile Ala Ser Ala Met Ala Val Glu Arg Ala Leu Ala Ile Arg 145 150 155160 Ala Pro His Trp Tyr Ala Ser His Met Lys Thr Arg Ala Thr Arg Ala 165170 175 Val Leu Leu Gly Val Trp Leu Ala Val Leu Ala Phe Ala Leu Leu Pro180 185 190 Val Leu Gly Val Gly Gln Tyr Thr Val Gln Trp Pro Gly Thr TrpCys 195 200 205 Phe Ile Ser Thr Gly Arg Gly Gly Asn Gly Thr Ser Ser SerHis Asn 210 215 220 Trp Gly Asn Leu Phe Phe Ala Ser Ala Phe Ala Phe LeuGly Leu Leu 225 230 235 240 Ala Leu Thr Val Thr Phe Ser Cys Asn Leu AlaThr Ile Lys Ala Leu 245 250 255 Val Ser Arg Cys Arg Ala Lys Ala Thr AlaSer Gln Ser Ser Ala Gln 260 265 270 Trp Gly Arg Ile Thr Thr Glu Thr AlaIle Gln Leu Met Gly Ile Met 275 280 285 Cys Val Leu Ser Val Cys Trp SerPro Leu Leu Ile Met Met Leu Lys 290 295 300 Met Ile Phe Asn Gln Thr SerVal Glu His Cys Lys Thr His Thr Glu 305 310 315 320 Lys Gln Lys Glu CysAsn Phe Phe Leu Ile Ala Val Arg Leu Ala Ser 325 330 335 Leu Asn Gln IleLeu Asp Pro Trp Val Tyr Leu Leu Leu Arg Lys Ile 340 345 350 Leu Leu ArgLys Phe Cys Gln Ile Arg Tyr His Thr Asn Asn Tyr Ala 355 360 365 Ser SerSer Thr Ser Leu Pro Cys Gln Cys Ser Ser Thr Leu Met Trp 370 375 380 SerAsp His Leu Glu Arg 385 390 23 1176 DNA Homo sapiens CDS (1)...(1170)cDNA 23 atg aag gag acc cgg ggc tac gga ggg gat gcc ccc ttc tgc acc cgc48 Met Lys Glu Thr Arg Gly Tyr Gly Gly Asp Ala Pro Phe Cys Thr Arg 1 510 15 ctc aac cac tcc tac aca ggc atg tgg gcg ccc gag cgt tcc gcc gag 96Leu Asn His Ser Tyr Thr Gly Met Trp Ala Pro Glu Arg Ser Ala Glu 20 25 30gcg cgg ggc aac ctc acg cgc cct cca ggg tct ggc gag gat tgc gga 144 AlaArg Gly Asn Leu Thr Arg Pro Pro Gly Ser Gly Glu Asp Cys Gly 35 40 45 tcggtg tcc gtg gcc ttc ccg atc acc atg ctg ctc act ggt ttc gtg 192 Ser ValSer Val Ala Phe Pro Ile Thr Met Leu Leu Thr Gly Phe Val 50 55 60 ggc aacgca ctg gcc atg ctg ctc gtg tcg cgc agc tac cgg cgc cgg 240 Gly Asn AlaLeu Ala Met Leu Leu Val Ser Arg Ser Tyr Arg Arg Arg 65 70 75 80 gag agcaag cgc aag aag tcc ttc ctg ctg tgc atc ggc tgg ctg gcg 288 Glu Ser LysArg Lys Lys Ser Phe Leu Leu Cys Ile Gly Trp Leu Ala 85 90 95 ctc acc gacctg gtc ggg cag ctt ctc acc acc ccg gtc gtc atc gtc 336 Leu Thr Asp LeuVal Gly Gln Leu Leu Thr Thr Pro Val Val Ile Val 100 105 110 gtg tac ctgtcc aag cag cgt tgg gag cac atc gac ccg tcg ggg cgg 384 Val Tyr Leu SerLys Gln Arg Trp Glu His Ile Asp Pro Ser Gly Arg 115 120 125 ctc tgc accttt ttc ggg ctg acc atg act gtt ttc ggg ctc tcc tcg 432 Leu Cys Thr PhePhe Gly Leu Thr Met Thr Val Phe Gly Leu Ser Ser 130 135 140 ttg ttc atcgcc agc gcc atg gcc gtc gag cgg gcg ctg gcc atc agg 480 Leu Phe Ile AlaSer Ala Met Ala Val Glu Arg Ala Leu Ala Ile Arg 145 150 155 160 gcg ccgcac tgg tat gcg agc cac atg aag acg cgt gcc acc cgc gct 528 Ala Pro HisTrp Tyr Ala Ser His Met Lys Thr Arg Ala Thr Arg Ala 165 170 175 gtg ctgctc ggc gtg tgg ctg gcc gtg ctc gcc ttc gcc ctg ctg ccg 576 Val Leu LeuGly Val Trp Leu Ala Val Leu Ala Phe Ala Leu Leu Pro 180 185 190 gtg ctgggc gtg ggc cag tac acc gtc cag tgg ccc ggg acg tgg tgc 624 Val Leu GlyVal Gly Gln Tyr Thr Val Gln Trp Pro Gly Thr Trp Cys 195 200 205 ttc atcagc acc ggg cga ggg ggc aac ggg act agc tct tcg cat aac 672 Phe Ile SerThr Gly Arg Gly Gly Asn Gly Thr Ser Ser Ser His Asn 210 215 220 tgg ggcaac ctt ttc ttc gcc tct gcc ttt gcc ttc ctg ggg ctc ttg 720 Trp Gly AsnLeu Phe Phe Ala Ser Ala Phe Ala Phe Leu Gly Leu Leu 225 230 235 240 gcgctg aca gtc acc ttt tcc tgc aac ctg gcc acc att aag gcc ctg 768 Ala LeuThr Val Thr Phe Ser Cys Asn Leu Ala Thr Ile Lys Ala Leu 245 250 255 gtgtcc cgc tgc cgg gcc aag gcc acg gca tct cag tcc agt gcc cag 816 Val SerArg Cys Arg Ala Lys Ala Thr Ala Ser Gln Ser Ser Ala Gln 260 265 270 tggggc cgc atc acg acc gag acg gcc att cag ctt atg ggg atc atg 864 Trp GlyArg Ile Thr Thr Glu Thr Ala Ile Gln Leu Met Gly Ile Met 275 280 285 tgcgtg ctg tcg gtc tgc tgg tct ccg ctc ctg ata atg atg ttg aaa 912 Cys ValLeu Ser Val Cys Trp Ser Pro Leu Leu Ile Met Met Leu Lys 290 295 300 atgatc ttc aat cag aca tca gtt gag cac tgc aag aca cac acg gag 960 Met IlePhe Asn Gln Thr Ser Val Glu His Cys Lys Thr His Thr Glu 305 310 315 320aag cag aaa gaa tgc aac ttc ttc tta ata gct gtt cgc ctg gct tca 1008 LysGln Lys Glu Cys Asn Phe Phe Leu Ile Ala Val Arg Leu Ala Ser 325 330 335ctg aac cag atc ttg gat cct tgg gtt tac ctg ctg tta aga aag atc 1056 LeuAsn Gln Ile Leu Asp Pro Trp Val Tyr Leu Leu Leu Arg Lys Ile 340 345 350ctt ctt cga aag ttt tgc cag atc agg tac cac aca aac aac tat gca 1104 LeuLeu Arg Lys Phe Cys Gln Ile Arg Tyr His Thr Asn Asn Tyr Ala 355 360 365tcc agc tcc acc tcc tta ccc tgc cag tgt tcc tca acc ttg atg tgg 1152 SerSer Ser Thr Ser Leu Pro Cys Gln Cys Ser Ser Thr Leu Met Trp 370 375 380agc gac cat ttg gaa aga taatga 1176 Ser Asp His Leu Glu Arg 385 390 24390 PRT Homo sapiens 24 Met Lys Glu Thr Arg Gly Tyr Gly Gly Asp Ala ProPhe Cys Thr Arg 1 5 10 15 Leu Asn His Ser Tyr Thr Gly Met Trp Ala ProGlu Arg Ser Ala Glu 20 25 30 Ala Arg Gly Asn Leu Thr Arg Pro Pro Gly SerGly Glu Asp Cys Gly 35 40 45 Ser Val Ser Val Ala Phe Pro Ile Thr Met LeuLeu Thr Gly Phe Val 50 55 60 Gly Asn Ala Leu Ala Met Leu Leu Val Ser ArgSer Tyr Arg Arg Arg 65 70 75 80 Glu Ser Lys Arg Lys Lys Ser Phe Leu LeuCys Ile Gly Trp Leu Ala 85 90 95 Leu Thr Asp Leu Val Gly Gln Leu Leu ThrThr Pro Val Val Ile Val 100 105 110 Val Tyr Leu Ser Lys Gln Arg Trp GluHis Ile Asp Pro Ser Gly Arg 115 120 125 Leu Cys Thr Phe Phe Gly Leu ThrMet Thr Val Phe Gly Leu Ser Ser 130 135 140 Leu Phe Ile Ala Ser Ala MetAla Val Glu Arg Ala Leu Ala Ile Arg 145 150 155 160 Ala Pro His Trp TyrAla Ser His Met Lys Thr Arg Ala Thr Arg Ala 165 170 175 Val Leu Leu GlyVal Trp Leu Ala Val Leu Ala Phe Ala Leu Leu Pro 180 185 190 Val Leu GlyVal Gly Gln Tyr Thr Val Gln Trp Pro Gly Thr Trp Cys 195 200 205 Phe IleSer Thr Gly Arg Gly Gly Asn Gly Thr Ser Ser Ser His Asn 210 215 220 TrpGly Asn Leu Phe Phe Ala Ser Ala Phe Ala Phe Leu Gly Leu Leu 225 230 235240 Ala Leu Thr Val Thr Phe Ser Cys Asn Leu Ala Thr Ile Lys Ala Leu 245250 255 Val Ser Arg Cys Arg Ala Lys Ala Thr Ala Ser Gln Ser Ser Ala Gln260 265 270 Trp Gly Arg Ile Thr Thr Glu Thr Ala Ile Gln Leu Met Gly IleMet 275 280 285 Cys Val Leu Ser Val Cys Trp Ser Pro Leu Leu Ile Met MetLeu Lys 290 295 300 Met Ile Phe Asn Gln Thr Ser Val Glu His Cys Lys ThrHis Thr Glu 305 310 315 320 Lys Gln Lys Glu Cys Asn Phe Phe Leu Ile AlaVal Arg Leu Ala Ser 325 330 335 Leu Asn Gln Ile Leu Asp Pro Trp Val TyrLeu Leu Leu Arg Lys Ile 340 345 350 Leu Leu Arg Lys Phe Cys Gln Ile ArgTyr His Thr Asn Asn Tyr Ala 355 360 365 Ser Ser Ser Thr Ser Leu Pro CysGln Cys Ser Ser Thr Leu Met Trp 370 375 380 Ser Asp His Leu Glu Arg 385390 25 1281 DNA Homo sapiens CDS (1)...(1275) cDNA 25 atg aag gag acccgg ggc tac gga ggg gat gcc ccc ttc tgc acc cgc 48 Met Lys Glu Thr ArgGly Tyr Gly Gly Asp Ala Pro Phe Cys Thr Arg 1 5 10 15 ctc aac cac tcctac aca ggc atg tgg gcg ccc gag cgt tcc gcc gag 96 Leu Asn His Ser TyrThr Gly Met Trp Ala Pro Glu Arg Ser Ala Glu 20 25 30 gcg cgg ggc aac ctcacg cgc cct cca ggg tct ggc gag gat tgc gga 144 Ala Arg Gly Asn Leu ThrArg Pro Pro Gly Ser Gly Glu Asp Cys Gly 35 40 45 tcg gtg tcc gtg gcc ttcccg atc acc atg ctg ctc act ggt ttc gtg 192 Ser Val Ser Val Ala Phe ProIle Thr Met Leu Leu Thr Gly Phe Val 50 55 60 ggc aac gca ctg gcc atg ctgctc gtg tcg cgc agc tac cgg cgc cgg 240 Gly Asn Ala Leu Ala Met Leu LeuVal Ser Arg Ser Tyr Arg Arg Arg 65 70 75 80 gag agc aag cgc aag aag tccttc ctg ctg tgc atc ggc tgg ctg gcg 288 Glu Ser Lys Arg Lys Lys Ser PheLeu Leu Cys Ile Gly Trp Leu Ala 85 90 95 ctc acc gac ctg gtc ggg cag cttctc acc acc ccg gtc gtc atc gtc 336 Leu Thr Asp Leu Val Gly Gln Leu LeuThr Thr Pro Val Val Ile Val 100 105 110 gtg tac ctg tcc aag cag cgt tgggag cac atc gac ccg tcg ggg cgg 384 Val Tyr Leu Ser Lys Gln Arg Trp GluHis Ile Asp Pro Ser Gly Arg 115 120 125 ctc tgc acc ttt ttc ggg ctg accatg act gtt ttc ggg ctc tcc tcg 432 Leu Cys Thr Phe Phe Gly Leu Thr MetThr Val Phe Gly Leu Ser Ser 130 135 140 ttg ttc atc gcc agc gcc atg gccgtc gag cgg gcg ctg gcc atc agg 480 Leu Phe Ile Ala Ser Ala Met Ala ValGlu Arg Ala Leu Ala Ile Arg 145 150 155 160 gcg ccg cac tgg tat gcg agccac atg aag acg cgt gcc acc cgc gct 528 Ala Pro His Trp Tyr Ala Ser HisMet Lys Thr Arg Ala Thr Arg Ala 165 170 175 gtg ctg ctc ggc gtg tgg ctggcc gtg ctc gcc ttc gcc ctg ctg ccg 576 Val Leu Leu Gly Val Trp Leu AlaVal Leu Ala Phe Ala Leu Leu Pro 180 185 190 gtg ctg ggc gtg ggc cag tacacc gtc cag tgg ccc ggg acg tgg tgc 624 Val Leu Gly Val Gly Gln Tyr ThrVal Gln Trp Pro Gly Thr Trp Cys 195 200 205 ttc atc agc acc ggg cga gggggc aac ggg act agc tct tcg cat aac 672 Phe Ile Ser Thr Gly Arg Gly GlyAsn Gly Thr Ser Ser Ser His Asn 210 215 220 tgg ggc aac ctt ttc ttc gcctct gcc ttt gcc ttc ctg ggg ctc ttg 720 Trp Gly Asn Leu Phe Phe Ala SerAla Phe Ala Phe Leu Gly Leu Leu 225 230 235 240 gcg ctg aca gtc acc ttttcc tgc aac ctg gcc acc att aag gcc ctg 768 Ala Leu Thr Val Thr Phe SerCys Asn Leu Ala Thr Ile Lys Ala Leu 245 250 255 gtg tcc cgc tgc cgg gccaag gcc acg gca tct cag tcc agt gcc cag 816 Val Ser Arg Cys Arg Ala LysAla Thr Ala Ser Gln Ser Ser Ala Gln 260 265 270 tgg ggc cgc atc acg accgag acg gcc att cag ctt atg ggg atc atg 864 Trp Gly Arg Ile Thr Thr GluThr Ala Ile Gln Leu Met Gly Ile Met 275 280 285 tgc gtg ctg tcg gtc tgctgg tct ccg ctc ctg ata atg atg ttg aaa 912 Cys Val Leu Ser Val Cys TrpSer Pro Leu Leu Ile Met Met Leu Lys 290 295 300 atg atc ttc aat cag acatca gtt gag cac tgc aag aca cac acg gag 960 Met Ile Phe Asn Gln Thr SerVal Glu His Cys Lys Thr His Thr Glu 305 310 315 320 aag cag aaa gaa tgcaac ttc ttc tta ata gct gtt cgc ctg gct tca 1008 Lys Gln Lys Glu Cys AsnPhe Phe Leu Ile Ala Val Arg Leu Ala Ser 325 330 335 ctg aac cag atc ttggat cct tgg gtt tac ctg ctg tta aga aag atc 1056 Leu Asn Gln Ile Leu AspPro Trp Val Tyr Leu Leu Leu Arg Lys Ile 340 345 350 ctt ctt cga aag ttttgc cag atg aga aaa aga aga ctc aga gag caa 1104 Leu Leu Arg Lys Phe CysGln Met Arg Lys Arg Arg Leu Arg Glu Gln 355 360 365 gct cct ctt ctt cccacc tct act gtg att gat cct tca agg ttc tgt 1152 Ala Pro Leu Leu Pro ThrSer Thr Val Ile Asp Pro Ser Arg Phe Cys 370 375 380 gct cag ccc ttc cgttgg ttc ttg gat ttg tcc ttt ccc gcc atg tct 1200 Ala Gln Pro Phe Arg TrpPhe Leu Asp Leu Ser Phe Pro Ala Met Ser 385 390 395 400 tca tca cat ccacaa ctt cca cta aca ctt gcg agc ttc aaa ctt ctt 1248 Ser Ser His Pro GlnLeu Pro Leu Thr Leu Ala Ser Phe Lys Leu Leu 405 410 415 aga gaa ccc tgcagt gtc cag cta agc tgatga 1281 Arg Glu Pro Cys Ser Val Gln Leu Ser 420425 26 425 PRT Homo sapiens 26 Met Lys Glu Thr Arg Gly Tyr Gly Gly AspAla Pro Phe Cys Thr Arg 1 5 10 15 Leu Asn His Ser Tyr Thr Gly Met TrpAla Pro Glu Arg Ser Ala Glu 20 25 30 Ala Arg Gly Asn Leu Thr Arg Pro ProGly Ser Gly Glu Asp Cys Gly 35 40 45 Ser Val Ser Val Ala Phe Pro Ile ThrMet Leu Leu Thr Gly Phe Val 50 55 60 Gly Asn Ala Leu Ala Met Leu Leu ValSer Arg Ser Tyr Arg Arg Arg 65 70 75 80 Glu Ser Lys Arg Lys Lys Ser PheLeu Leu Cys Ile Gly Trp Leu Ala 85 90 95 Leu Thr Asp Leu Val Gly Gln LeuLeu Thr Thr Pro Val Val Ile Val 100 105 110 Val Tyr Leu Ser Lys Gln ArgTrp Glu His Ile Asp Pro Ser Gly Arg 115 120 125 Leu Cys Thr Phe Phe GlyLeu Thr Met Thr Val Phe Gly Leu Ser Ser 130 135 140 Leu Phe Ile Ala SerAla Met Ala Val Glu Arg Ala Leu Ala Ile Arg 145 150 155 160 Ala Pro HisTrp Tyr Ala Ser His Met Lys Thr Arg Ala Thr Arg Ala 165 170 175 Val LeuLeu Gly Val Trp Leu Ala Val Leu Ala Phe Ala Leu Leu Pro 180 185 190 ValLeu Gly Val Gly Gln Tyr Thr Val Gln Trp Pro Gly Thr Trp Cys 195 200 205Phe Ile Ser Thr Gly Arg Gly Gly Asn Gly Thr Ser Ser Ser His Asn 210 215220 Trp Gly Asn Leu Phe Phe Ala Ser Ala Phe Ala Phe Leu Gly Leu Leu 225230 235 240 Ala Leu Thr Val Thr Phe Ser Cys Asn Leu Ala Thr Ile Lys AlaLeu 245 250 255 Val Ser Arg Cys Arg Ala Lys Ala Thr Ala Ser Gln Ser SerAla Gln 260 265 270 Trp Gly Arg Ile Thr Thr Glu Thr Ala Ile Gln Leu MetGly Ile Met 275 280 285 Cys Val Leu Ser Val Cys Trp Ser Pro Leu Leu IleMet Met Leu Lys 290 295 300 Met Ile Phe Asn Gln Thr Ser Val Glu His CysLys Thr His Thr Glu 305 310 315 320 Lys Gln Lys Glu Cys Asn Phe Phe LeuIle Ala Val Arg Leu Ala Ser 325 330 335 Leu Asn Gln Ile Leu Asp Pro TrpVal Tyr Leu Leu Leu Arg Lys Ile 340 345 350 Leu Leu Arg Lys Phe Cys GlnMet Arg Lys Arg Arg Leu Arg Glu Gln 355 360 365 Ala Pro Leu Leu Pro ThrSer Thr Val Ile Asp Pro Ser Arg Phe Cys 370 375 380 Ala Gln Pro Phe ArgTrp Phe Leu Asp Leu Ser Phe Pro Ala Met Ser 385 390 395 400 Ser Ser HisPro Gln Leu Pro Leu Thr Leu Ala Ser Phe Lys Leu Leu 405 410 415 Arg GluPro Cys Ser Val Gln Leu Ser 420 425 27 1182 DNA Homo sapiens CDS(1)...(1179) cDNA 27 atg aag gag acc cgg ggc tac gga ggg gat gcc ccc ttctgc acc cgc 48 Met Lys Glu Thr Arg Gly Tyr Gly Gly Asp Ala Pro Phe CysThr Arg 1 5 10 15 ctc aac cac tcc tac aca ggc atg tgg gcg ccc gag cgttcc gcc gag 96 Leu Asn His Ser Tyr Thr Gly Met Trp Ala Pro Glu Arg SerAla Glu 20 25 30 gcg cgg ggc aac ctc acg cgc cct cca ggg tct ggc gag gattgc gga 144 Ala Arg Gly Asn Leu Thr Arg Pro Pro Gly Ser Gly Glu Asp CysGly 35 40 45 tcg gtg tcc gtg gcc ttc ccg atc acc atg ctg ctc act ggt ttcgtg 192 Ser Val Ser Val Ala Phe Pro Ile Thr Met Leu Leu Thr Gly Phe Val50 55 60 ggc aac gca ctg gcc atg ctg ctc gtg tcg cgc agc tac cgg cgc cgg240 Gly Asn Ala Leu Ala Met Leu Leu Val Ser Arg Ser Tyr Arg Arg Arg 6570 75 80 gag agc aag cgc aag aag tcc ttc ctg ctg tgc atc ggc tgg ctg gcg288 Glu Ser Lys Arg Lys Lys Ser Phe Leu Leu Cys Ile Gly Trp Leu Ala 8590 95 ctc acc gac ctg gtc ggg cag ctt ctc acc acc ccg gtc gtc atc gtc336 Leu Thr Asp Leu Val Gly Gln Leu Leu Thr Thr Pro Val Val Ile Val 100105 110 gtg tac ctg tcc aag cag cgt tgg gag cac atc gac ccg tcg ggg cgg384 Val Tyr Leu Ser Lys Gln Arg Trp Glu His Ile Asp Pro Ser Gly Arg 115120 125 ctc tgc acc ttt ttc ggg ctg acc atg act gtt ttc ggg ctc tcc tcg432 Leu Cys Thr Phe Phe Gly Leu Thr Met Thr Val Phe Gly Leu Ser Ser 130135 140 ttg ttc atc gcc agc gcc atg gcc gtc gag cgg gcg ctg gcc atc agg480 Leu Phe Ile Ala Ser Ala Met Ala Val Glu Arg Ala Leu Ala Ile Arg 145150 155 160 gcg ccg cac tgg tat gcg agc cac atg aag acg cgt gcc acc cgcgct 528 Ala Pro His Trp Tyr Ala Ser His Met Lys Thr Arg Ala Thr Arg Ala165 170 175 gtg ctg ctc ggc gtg tgg ctg gcc gtg ctc gcc ttc gcc ctg ctgccg 576 Val Leu Leu Gly Val Trp Leu Ala Val Leu Ala Phe Ala Leu Leu Pro180 185 190 gtg ctg ggc gtg ggc cag tac acc gtc cag tgg ccc ggg acg tggtgc 624 Val Leu Gly Val Gly Gln Tyr Thr Val Gln Trp Pro Gly Thr Trp Cys195 200 205 ttc atc agc acc ggg cga ggg ggc aac ggg act agc tct tcg cataac 672 Phe Ile Ser Thr Gly Arg Gly Gly Asn Gly Thr Ser Ser Ser His Asn210 215 220 tgg ggc aac ctt ttc ttc gcc tct gcc ttt gcc ttc ctg ggg ctcttg 720 Trp Gly Asn Leu Phe Phe Ala Ser Ala Phe Ala Phe Leu Gly Leu Leu225 230 235 240 gcg ctg aca gtc acc ttt tcc tgc aac ctg gcc acc att aaggcc ctg 768 Ala Leu Thr Val Thr Phe Ser Cys Asn Leu Ala Thr Ile Lys AlaLeu 245 250 255 gtg tcc cgc tgc cgg gcc aag gcc acg gca tct cag tcc agtgcc cag 816 Val Ser Arg Cys Arg Ala Lys Ala Thr Ala Ser Gln Ser Ser AlaGln 260 265 270 tgg ggc cgc atc acg acc gag acg gcc att cag ctt atg gggatc atg 864 Trp Gly Arg Ile Thr Thr Glu Thr Ala Ile Gln Leu Met Gly IleMet 275 280 285 tgc gtg ctg tcg gtc tgc tgg tct ccg ctc ctg ata atg atgttg aaa 912 Cys Val Leu Ser Val Cys Trp Ser Pro Leu Leu Ile Met Met LeuLys 290 295 300 atg atc ttc aat cag aca tca gtt gag cac tgc aag aca cacacg gag 960 Met Ile Phe Asn Gln Thr Ser Val Glu His Cys Lys Thr His ThrGlu 305 310 315 320 aag cag aaa gaa tgc aac ttc ttc tta ata gct gtt cgcctg gct tca 1008 Lys Gln Lys Glu Cys Asn Phe Phe Leu Ile Ala Val Arg LeuAla Ser 325 330 335 ctg aac cag atc ttg gat cct tgg gtt tac ctg ctg ttaaga aag atc 1056 Leu Asn Gln Ile Leu Asp Pro Trp Val Tyr Leu Leu Leu ArgLys Ile 340 345 350 ctt ctt cga aag ttt tgc cag atg aga aaa aga aga ctcaga gag caa 1104 Leu Leu Arg Lys Phe Cys Gln Met Arg Lys Arg Arg Leu ArgGlu Gln 355 360 365 tta atc tgc agt ttg cga act ctc aga tac aga ggg caactg cac att 1152 Leu Ile Cys Ser Leu Arg Thr Leu Arg Tyr Arg Gly Gln LeuHis Ile 370 375 380 gtg ggc aag tac aaa cct atc gtg tgc tga 1182 Val GlyLys Tyr Lys Pro Ile Val Cys 385 390 28 393 PRT Homo sapiens 28 Met LysGlu Thr Arg Gly Tyr Gly Gly Asp Ala Pro Phe Cys Thr Arg 1 5 10 15 LeuAsn His Ser Tyr Thr Gly Met Trp Ala Pro Glu Arg Ser Ala Glu 20 25 30 AlaArg Gly Asn Leu Thr Arg Pro Pro Gly Ser Gly Glu Asp Cys Gly 35 40 45 SerVal Ser Val Ala Phe Pro Ile Thr Met Leu Leu Thr Gly Phe Val 50 55 60 GlyAsn Ala Leu Ala Met Leu Leu Val Ser Arg Ser Tyr Arg Arg Arg 65 70 75 80Glu Ser Lys Arg Lys Lys Ser Phe Leu Leu Cys Ile Gly Trp Leu Ala 85 90 95Leu Thr Asp Leu Val Gly Gln Leu Leu Thr Thr Pro Val Val Ile Val 100 105110 Val Tyr Leu Ser Lys Gln Arg Trp Glu His Ile Asp Pro Ser Gly Arg 115120 125 Leu Cys Thr Phe Phe Gly Leu Thr Met Thr Val Phe Gly Leu Ser Ser130 135 140 Leu Phe Ile Ala Ser Ala Met Ala Val Glu Arg Ala Leu Ala IleArg 145 150 155 160 Ala Pro His Trp Tyr Ala Ser His Met Lys Thr Arg AlaThr Arg Ala 165 170 175 Val Leu Leu Gly Val Trp Leu Ala Val Leu Ala PheAla Leu Leu Pro 180 185 190 Val Leu Gly Val Gly Gln Tyr Thr Val Gln TrpPro Gly Thr Trp Cys 195 200 205 Phe Ile Ser Thr Gly Arg Gly Gly Asn GlyThr Ser Ser Ser His Asn 210 215 220 Trp Gly Asn Leu Phe Phe Ala Ser AlaPhe Ala Phe Leu Gly Leu Leu 225 230 235 240 Ala Leu Thr Val Thr Phe SerCys Asn Leu Ala Thr Ile Lys Ala Leu 245 250 255 Val Ser Arg Cys Arg AlaLys Ala Thr Ala Ser Gln Ser Ser Ala Gln 260 265 270 Trp Gly Arg Ile ThrThr Glu Thr Ala Ile Gln Leu Met Gly Ile Met 275 280 285 Cys Val Leu SerVal Cys Trp Ser Pro Leu Leu Ile Met Met Leu Lys 290 295 300 Met Ile PheAsn Gln Thr Ser Val Glu His Cys Lys Thr His Thr Glu 305 310 315 320 LysGln Lys Glu Cys Asn Phe Phe Leu Ile Ala Val Arg Leu Ala Ser 325 330 335Leu Asn Gln Ile Leu Asp Pro Trp Val Tyr Leu Leu Leu Arg Lys Ile 340 345350 Leu Leu Arg Lys Phe Cys Gln Met Arg Lys Arg Arg Leu Arg Glu Gln 355360 365 Leu Ile Cys Ser Leu Arg Thr Leu Arg Tyr Arg Gly Gln Leu His Ile370 375 380 Val Gly Lys Tyr Lys Pro Ile Val Cys 385 390 29 1125 DNA Homosapiens CDS (1)...(1122) cDNA 29 atg aag gag acc cgg ggc tac gga ggg gatgcc ccc ttc tgc acc cgc 48 Met Lys Glu Thr Arg Gly Tyr Gly Gly Asp AlaPro Phe Cys Thr Arg 1 5 10 15 ctc aac cac tcc tac aca ggc atg tgg gcgccc gag cgt tcc gcc gag 96 Leu Asn His Ser Tyr Thr Gly Met Trp Ala ProGlu Arg Ser Ala Glu 20 25 30 gcg cgg ggc aac ctc acg cgc cct cca ggg tctggc gag gat tgc gga 144 Ala Arg Gly Asn Leu Thr Arg Pro Pro Gly Ser GlyGlu Asp Cys Gly 35 40 45 tcg gtg tcc gtg gcc ttc ccg atc acc atg ctg ctcact ggt ttc gtg 192 Ser Val Ser Val Ala Phe Pro Ile Thr Met Leu Leu ThrGly Phe Val 50 55 60 ggc aac gca ctg gcc atg ctg ctc gtg tcg cgc agc taccgg cgc cgg 240 Gly Asn Ala Leu Ala Met Leu Leu Val Ser Arg Ser Tyr ArgArg Arg 65 70 75 80 gag agc aag cgc aag aag tcc ttc ctg ctg tgc atc ggctgg ctg gcg 288 Glu Ser Lys Arg Lys Lys Ser Phe Leu Leu Cys Ile Gly TrpLeu Ala 85 90 95 ctc acc gac ctg gtc ggg cag ctt ctc acc acc ccg gtc gtcatc gtc 336 Leu Thr Asp Leu Val Gly Gln Leu Leu Thr Thr Pro Val Val IleVal 100 105 110 gtg tac ctg tcc aag cag cgt tgg gag cac atc gac ccg tcgggg cgg 384 Val Tyr Leu Ser Lys Gln Arg Trp Glu His Ile Asp Pro Ser GlyArg 115 120 125 ctc tgc acc ttt ttc ggg ctg acc atg act gtt ttc ggg ctctcc tcg 432 Leu Cys Thr Phe Phe Gly Leu Thr Met Thr Val Phe Gly Leu SerSer 130 135 140 ttg ttc atc gcc agc gcc atg gcc gtc gag cgg gcg ctg gccatc agg 480 Leu Phe Ile Ala Ser Ala Met Ala Val Glu Arg Ala Leu Ala IleArg 145 150 155 160 gcg ccg cac tgg tat gcg agc cac atg aag acg cgt gccacc cgc gct 528 Ala Pro His Trp Tyr Ala Ser His Met Lys Thr Arg Ala ThrArg Ala 165 170 175 gtg ctg ctc ggc gtg tgg ctg gcc gtg ctc gcc ttc gccctg ctg ccg 576 Val Leu Leu Gly Val Trp Leu Ala Val Leu Ala Phe Ala LeuLeu Pro 180 185 190 gtg ctg ggc gtg ggc cag tac acc gtc cag tgg ccc gggacg tgg tgc 624 Val Leu Gly Val Gly Gln Tyr Thr Val Gln Trp Pro Gly ThrTrp Cys 195 200 205 ttc atc agc acc ggg cga ggg ggc aac ggg act agc tcttcg cat aac 672 Phe Ile Ser Thr Gly Arg Gly Gly Asn Gly Thr Ser Ser SerHis Asn 210 215 220 tgg ggc aac ctt ttc ttc gcc tct gcc ttt gcc ttc ctgggg ctc ttg 720 Trp Gly Asn Leu Phe Phe Ala Ser Ala Phe Ala Phe Leu GlyLeu Leu 225 230 235 240 gcg ctg aca gtc acc ttt tcc tgc aac ctg gcc accatt aag gcc ctg 768 Ala Leu Thr Val Thr Phe Ser Cys Asn Leu Ala Thr IleLys Ala Leu 245 250 255 gtg tcc cgc tgc cgg gcc aag gcc acg gca tct cagtcc agt gcc cag 816 Val Ser Arg Cys Arg Ala Lys Ala Thr Ala Ser Gln SerSer Ala Gln 260 265 270 tgg ggc cgc atc acg acc gag acg gcc att cag cttatg ggg atc atg 864 Trp Gly Arg Ile Thr Thr Glu Thr Ala Ile Gln Leu MetGly Ile Met 275 280 285 tgc gtg ctg tcg gtc tgc tgg tct ccg ctc ctg ataatg atg ttg aaa 912 Cys Val Leu Ser Val Cys Trp Ser Pro Leu Leu Ile MetMet Leu Lys 290 295 300 atg atc ttc aat cag aca tca gtt gag cac tgc aagaca cac acg gag 960 Met Ile Phe Asn Gln Thr Ser Val Glu His Cys Lys ThrHis Thr Glu 305 310 315 320 aag cag aaa gaa tgc aac ttc ttc tta ata gctgtt cgc ctg gct tca 1008 Lys Gln Lys Glu Cys Asn Phe Phe Leu Ile Ala ValArg Leu Ala Ser 325 330 335 ctg aac cag atc ttg gat cct tgg gtt tac ctgctg tta aga aag atc 1056 Leu Asn Gln Ile Leu Asp Pro Trp Val Tyr Leu LeuLeu Arg Lys Ile 340 345 350 ctt ctt cga aag ttt tgc cag atg aga aaa agaaga ctc aga gag caa 1104 Leu Leu Arg Lys Phe Cys Gln Met Arg Lys Arg ArgLeu Arg Glu Gln 355 360 365 gag gaa ttt tgg gga aat taa 1125 Glu Glu PheTrp Gly Asn 370 30 374 PRT Homo sapiens 30 Met Lys Glu Thr Arg Gly TyrGly Gly Asp Ala Pro Phe Cys Thr Arg 1 5 10 15 Leu Asn His Ser Tyr ThrGly Met Trp Ala Pro Glu Arg Ser Ala Glu 20 25 30 Ala Arg Gly Asn Leu ThrArg Pro Pro Gly Ser Gly Glu Asp Cys Gly 35 40 45 Ser Val Ser Val Ala PhePro Ile Thr Met Leu Leu Thr Gly Phe Val 50 55 60 Gly Asn Ala Leu Ala MetLeu Leu Val Ser Arg Ser Tyr Arg Arg Arg 65 70 75 80 Glu Ser Lys Arg LysLys Ser Phe Leu Leu Cys Ile Gly Trp Leu Ala 85 90 95 Leu Thr Asp Leu ValGly Gln Leu Leu Thr Thr Pro Val Val Ile Val 100 105 110 Val Tyr Leu SerLys Gln Arg Trp Glu His Ile Asp Pro Ser Gly Arg 115 120 125 Leu Cys ThrPhe Phe Gly Leu Thr Met Thr Val Phe Gly Leu Ser Ser 130 135 140 Leu PheIle Ala Ser Ala Met Ala Val Glu Arg Ala Leu Ala Ile Arg 145 150 155 160Ala Pro His Trp Tyr Ala Ser His Met Lys Thr Arg Ala Thr Arg Ala 165 170175 Val Leu Leu Gly Val Trp Leu Ala Val Leu Ala Phe Ala Leu Leu Pro 180185 190 Val Leu Gly Val Gly Gln Tyr Thr Val Gln Trp Pro Gly Thr Trp Cys195 200 205 Phe Ile Ser Thr Gly Arg Gly Gly Asn Gly Thr Ser Ser Ser HisAsn 210 215 220 Trp Gly Asn Leu Phe Phe Ala Ser Ala Phe Ala Phe Leu GlyLeu Leu 225 230 235 240 Ala Leu Thr Val Thr Phe Ser Cys Asn Leu Ala ThrIle Lys Ala Leu 245 250 255 Val Ser Arg Cys Arg Ala Lys Ala Thr Ala SerGln Ser Ser Ala Gln 260 265 270 Trp Gly Arg Ile Thr Thr Glu Thr Ala IleGln Leu Met Gly Ile Met 275 280 285 Cys Val Leu Ser Val Cys Trp Ser ProLeu Leu Ile Met Met Leu Lys 290 295 300 Met Ile Phe Asn Gln Thr Ser ValGlu His Cys Lys Thr His Thr Glu 305 310 315 320 Lys Gln Lys Glu Cys AsnPhe Phe Leu Ile Ala Val Arg Leu Ala Ser 325 330 335 Leu Asn Gln Ile LeuAsp Pro Trp Val Tyr Leu Leu Leu Arg Lys Ile 340 345 350 Leu Leu Arg LysPhe Cys Gln Met Arg Lys Arg Arg Leu Arg Glu Gln 355 360 365 Glu Glu PheTrp Gly Asn 370 31 1098 DNA Homo sapiens CDS (1)...(1095) cDNA 31 atgaag gag acc cgg ggc tac gga ggg gat gcc ccc ttc tgc acc cgc 48 Met LysGlu Thr Arg Gly Tyr Gly Gly Asp Ala Pro Phe Cys Thr Arg 1 5 10 15 ctcaac cac tcc tac aca ggc atg tgg gcg ccc gag cgt tcc gcc gag 96 Leu AsnHis Ser Tyr Thr Gly Met Trp Ala Pro Glu Arg Ser Ala Glu 20 25 30 gcg cggggc aac ctc acg cgc cct cca ggg tct ggc gag gat tgc gga 144 Ala Arg GlyAsn Leu Thr Arg Pro Pro Gly Ser Gly Glu Asp Cys Gly 35 40 45 tcg gtg tccgtg gcc ttc ccg atc acc atg ctg ctc act ggt ttc gtg 192 Ser Val Ser ValAla Phe Pro Ile Thr Met Leu Leu Thr Gly Phe Val 50 55 60 ggc aac gca ctggcc atg ctg ctc gtg tcg cgc agc tac cgg cgc cgg 240 Gly Asn Ala Leu AlaMet Leu Leu Val Ser Arg Ser Tyr Arg Arg Arg 65 70 75 80 gag agc aag cgcaag aag tcc ttc ctg ctg tgc atc ggc tgg ctg gcg 288 Glu Ser Lys Arg LysLys Ser Phe Leu Leu Cys Ile Gly Trp Leu Ala 85 90 95 ctc acc gac ctg gtcggg cag ctt ctc acc acc ccg gtc gtc atc gtc 336 Leu Thr Asp Leu Val GlyGln Leu Leu Thr Thr Pro Val Val Ile Val 100 105 110 gtg tac ctg tcc aagcag cgt tgg gag cac atc gac ccg tcg ggg cgg 384 Val Tyr Leu Ser Lys GlnArg Trp Glu His Ile Asp Pro Ser Gly Arg 115 120 125 ctc tgc acc ttt ttcggg ctg acc atg act gtt ttc ggg ctc tcc tcg 432 Leu Cys Thr Phe Phe GlyLeu Thr Met Thr Val Phe Gly Leu Ser Ser 130 135 140 ttg ttc atc gcc agcgcc atg gcc gtc gag cgg gcg ctg gcc atc agg 480 Leu Phe Ile Ala Ser AlaMet Ala Val Glu Arg Ala Leu Ala Ile Arg 145 150 155 160 gcg ccg cac tggtat gcg agc cac atg aag acg cgt gcc acc cgc gct 528 Ala Pro His Trp TyrAla Ser His Met Lys Thr Arg Ala Thr Arg Ala 165 170 175 gtg ctg ctc ggcgtg tgg ctg gcc gtg ctc gcc ttc gcc ctg ctg ccg 576 Val Leu Leu Gly ValTrp Leu Ala Val Leu Ala Phe Ala Leu Leu Pro 180 185 190 gtg ctg ggc gtgggc cag tac acc gtc cag tgg ccc ggg acg tgg tgc 624 Val Leu Gly Val GlyGln Tyr Thr Val Gln Trp Pro Gly Thr Trp Cys 195 200 205 ttc atc agc accggg cga ggg ggc aac ggg act agc tct tcg cat aac 672 Phe Ile Ser Thr GlyArg Gly Gly Asn Gly Thr Ser Ser Ser His Asn 210 215 220 tgg ggc aac cttttc ttc gcc tct gcc ttt gcc ttc ctg ggg ctc ttg 720 Trp Gly Asn Leu PhePhe Ala Ser Ala Phe Ala Phe Leu Gly Leu Leu 225 230 235 240 gcg ctg acagtc acc ttt tcc tgc aac ctg gcc acc att aag gcc ctg 768 Ala Leu Thr ValThr Phe Ser Cys Asn Leu Ala Thr Ile Lys Ala Leu 245 250 255 gtg tcc cgctgc cgg gcc aag gcc acg gca tct cag tcc agt gcc cag 816 Val Ser Arg CysArg Ala Lys Ala Thr Ala Ser Gln Ser Ser Ala Gln 260 265 270 tgg ggc cgcatc acg acc gag acg gcc att cag ctt atg ggg atc atg 864 Trp Gly Arg IleThr Thr Glu Thr Ala Ile Gln Leu Met Gly Ile Met 275 280 285 tgc gtg ctgtcg gtc tgc tgg tct ccg ctc ctg ata atg atg ttg aaa 912 Cys Val Leu SerVal Cys Trp Ser Pro Leu Leu Ile Met Met Leu Lys 290 295 300 atg atc ttcaat cag aca tca gtt gag cac tgc aag aca cac acg gag 960 Met Ile Phe AsnGln Thr Ser Val Glu His Cys Lys Thr His Thr Glu 305 310 315 320 aag cagaaa gaa tgc aac ttc ttc tta ata gct gtt cgc ctg gct tca 1008 Lys Gln LysGlu Cys Asn Phe Phe Leu Ile Ala Val Arg Leu Ala Ser 325 330 335 ctg aaccag atc ttg gat cct tgg gtt tac ctg ctg tta aga aag atc 1056 Leu Asn GlnIle Leu Asp Pro Trp Val Tyr Leu Leu Leu Arg Lys Ile 340 345 350 ctt cttcga aag ttt tgc cag gag gaa ttt tgg gga aat taa 1098 Leu Leu Arg Lys PheCys Gln Glu Glu Phe Trp Gly Asn 355 360 365 32 365 PRT Homo sapiens 32Met Lys Glu Thr Arg Gly Tyr Gly Gly Asp Ala Pro Phe Cys Thr Arg 1 5 1015 Leu Asn His Ser Tyr Thr Gly Met Trp Ala Pro Glu Arg Ser Ala Glu 20 2530 Ala Arg Gly Asn Leu Thr Arg Pro Pro Gly Ser Gly Glu Asp Cys Gly 35 4045 Ser Val Ser Val Ala Phe Pro Ile Thr Met Leu Leu Thr Gly Phe Val 50 5560 Gly Asn Ala Leu Ala Met Leu Leu Val Ser Arg Ser Tyr Arg Arg Arg 65 7075 80 Glu Ser Lys Arg Lys Lys Ser Phe Leu Leu Cys Ile Gly Trp Leu Ala 8590 95 Leu Thr Asp Leu Val Gly Gln Leu Leu Thr Thr Pro Val Val Ile Val100 105 110 Val Tyr Leu Ser Lys Gln Arg Trp Glu His Ile Asp Pro Ser GlyArg 115 120 125 Leu Cys Thr Phe Phe Gly Leu Thr Met Thr Val Phe Gly LeuSer Ser 130 135 140 Leu Phe Ile Ala Ser Ala Met Ala Val Glu Arg Ala LeuAla Ile Arg 145 150 155 160 Ala Pro His Trp Tyr Ala Ser His Met Lys ThrArg Ala Thr Arg Ala 165 170 175 Val Leu Leu Gly Val Trp Leu Ala Val LeuAla Phe Ala Leu Leu Pro 180 185 190 Val Leu Gly Val Gly Gln Tyr Thr ValGln Trp Pro Gly Thr Trp Cys 195 200 205 Phe Ile Ser Thr Gly Arg Gly GlyAsn Gly Thr Ser Ser Ser His Asn 210 215 220 Trp Gly Asn Leu Phe Phe AlaSer Ala Phe Ala Phe Leu Gly Leu Leu 225 230 235 240 Ala Leu Thr Val ThrPhe Ser Cys Asn Leu Ala Thr Ile Lys Ala Leu 245 250 255 Val Ser Arg CysArg Ala Lys Ala Thr Ala Ser Gln Ser Ser Ala Gln 260 265 270 Trp Gly ArgIle Thr Thr Glu Thr Ala Ile Gln Leu Met Gly Ile Met 275 280 285 Cys ValLeu Ser Val Cys Trp Ser Pro Leu Leu Ile Met Met Leu Lys 290 295 300 MetIle Phe Asn Gln Thr Ser Val Glu His Cys Lys Thr His Thr Glu 305 310 315320 Lys Gln Lys Glu Cys Asn Phe Phe Leu Ile Ala Val Arg Leu Ala Ser 325330 335 Leu Asn Gln Ile Leu Asp Pro Trp Val Tyr Leu Leu Leu Arg Lys Ile340 345 350 Leu Leu Arg Lys Phe Cys Gln Glu Glu Phe Trp Gly Asn 355 360365 33 1212 DNA Homo sapiens CDS (1)...(1206) cDNA 33 atg aag gag acccgg ggc tac gga ggg gat gcc ccc ttc tgc acc cgc 48 Met Lys Glu Thr ArgGly Tyr Gly Gly Asp Ala Pro Phe Cys Thr Arg 1 5 10 15 ctc aac cac tcctac aca ggc atg tgg gcg ccc gag cgt tcc gcc gag 96 Leu Asn His Ser TyrThr Gly Met Trp Ala Pro Glu Arg Ser Ala Glu 20 25 30 gcg cgg ggc aac ctcacg cgc cct cca ggg tct ggc gag gat tgc gga 144 Ala Arg Gly Asn Leu ThrArg Pro Pro Gly Ser Gly Glu Asp Cys Gly 35 40 45 tcg gtg tcc gtg gcc ttcccg atc acc atg ctg ctc act ggt ttc gtg 192 Ser Val Ser Val Ala Phe ProIle Thr Met Leu Leu Thr Gly Phe Val 50 55 60 ggc aac gca ctg gcc atg ctgctc gtg tcg cgc agc tac cgg cgc cgg 240 Gly Asn Ala Leu Ala Met Leu LeuVal Ser Arg Ser Tyr Arg Arg Arg 65 70 75 80 gag agc aag cgc aag aag tccttc ctg ctg tgc atc ggc tgg ctg gcg 288 Glu Ser Lys Arg Lys Lys Ser PheLeu Leu Cys Ile Gly Trp Leu Ala 85 90 95 ctc acc gac ctg gtc ggg cag cttctc acc acc ccg gtc gtc atc gtc 336 Leu Thr Asp Leu Val Gly Gln Leu LeuThr Thr Pro Val Val Ile Val 100 105 110 gtg tac ctg tcc aag cag cgt tgggag cac atc gac ccg tcg ggg cgg 384 Val Tyr Leu Ser Lys Gln Arg Trp GluHis Ile Asp Pro Ser Gly Arg 115 120 125 ctc tgc acc ttt ttc ggg ctg accatg act gtt ttc ggg ctc tcc tcg 432 Leu Cys Thr Phe Phe Gly Leu Thr MetThr Val Phe Gly Leu Ser Ser 130 135 140 ttg ttc atc gcc agc gcc atg gccgtc gag cgg gcg ctg gcc atc agg 480 Leu Phe Ile Ala Ser Ala Met Ala ValGlu Arg Ala Leu Ala Ile Arg 145 150 155 160 gcg ccg cac tgg tat gcg agccac atg aag acg cgt gcc acc cgc gct 528 Ala Pro His Trp Tyr Ala Ser HisMet Lys Thr Arg Ala Thr Arg Ala 165 170 175 gtg ctg ctc ggc gtg tgg ctggcc gtg ctc gcc ttc gcc ctg ctg ccg 576 Val Leu Leu Gly Val Trp Leu AlaVal Leu Ala Phe Ala Leu Leu Pro 180 185 190 gtg ctg ggc gtg ggc cag tacacc gtc cag tgg ccc ggg acg tgg tgc 624 Val Leu Gly Val Gly Gln Tyr ThrVal Gln Trp Pro Gly Thr Trp Cys 195 200 205 ttc atc agc acc ggg cga gggggc aac ggg act agc tct tcg cat aac 672 Phe Ile Ser Thr Gly Arg Gly GlyAsn Gly Thr Ser Ser Ser His Asn 210 215 220 tgg ggc aac ctt ttc ttc gcctct gcc ttt gcc ttc ctg ggg ctc ttg 720 Trp Gly Asn Leu Phe Phe Ala SerAla Phe Ala Phe Leu Gly Leu Leu 225 230 235 240 gcg ctg aca gtc acc ttttcc tgc aac ctg gcc acc att aag gcc ctg 768 Ala Leu Thr Val Thr Phe SerCys Asn Leu Ala Thr Ile Lys Ala Leu 245 250 255 gtg tcc cgc tgc cgg gccaag gcc acg gca tct cag tcc agt gcc cag 816 Val Ser Arg Cys Arg Ala LysAla Thr Ala Ser Gln Ser Ser Ala Gln 260 265 270 tgg ggc cgc atc acg accgag acg gcc att cag ctt atg ggg atc atg 864 Trp Gly Arg Ile Thr Thr GluThr Ala Ile Gln Leu Met Gly Ile Met 275 280 285 tgc gtg ctg tcg gtc tgctgg tct ccg ctc ctg ata atg atg ttg aaa 912 Cys Val Leu Ser Val Cys TrpSer Pro Leu Leu Ile Met Met Leu Lys 290 295 300 atg atc ttc aat cag acatca gtt gag cac tgc aag aca cac acg gag 960 Met Ile Phe Asn Gln Thr SerVal Glu His Cys Lys Thr His Thr Glu 305 310 315 320 aag cag aaa gaa tgcaac ttc ttc tta ata gct gtt cgc ctg gct tca 1008 Lys Gln Lys Glu Cys AsnPhe Phe Leu Ile Ala Val Arg Leu Ala Ser 325 330 335 ctg aac cag atc ttggat cct tgg gtt tac ctg ctg tta aga aag atc 1056 Leu Asn Gln Ile Leu AspPro Trp Val Tyr Leu Leu Leu Arg Lys Ile 340 345 350 ctt ctt cga aag ttttgc cag atg aga aaa aga aga ctc aga gag caa 1104 Leu Leu Arg Lys Phe CysGln Met Arg Lys Arg Arg Leu Arg Glu Gln 355 360 365 gag atg ggg cct gatgga agg tgt ttt tgt cat gca tgg agg cag gtc 1152 Glu Met Gly Pro Asp GlyArg Cys Phe Cys His Ala Trp Arg Gln Val 370 375 380 ccc agg act tgg tgcagt tct cat gat aga gaa ccc tgc agt gtc cag 1200 Pro Arg Thr Trp Cys SerSer His Asp Arg Glu Pro Cys Ser Val Gln 385 390 395 400 cta agc tgatga1212 Leu Ser 34 402 PRT Homo sapiens 34 Met Lys Glu Thr Arg Gly Tyr GlyGly Asp Ala Pro Phe Cys Thr Arg 1 5 10 15 Leu Asn His Ser Tyr Thr GlyMet Trp Ala Pro Glu Arg Ser Ala Glu 20 25 30 Ala Arg Gly Asn Leu Thr ArgPro Pro Gly Ser Gly Glu Asp Cys Gly 35 40 45 Ser Val Ser Val Ala Phe ProIle Thr Met Leu Leu Thr Gly Phe Val 50 55 60 Gly Asn Ala Leu Ala Met LeuLeu Val Ser Arg Ser Tyr Arg Arg Arg 65 70 75 80 Glu Ser Lys Arg Lys LysSer Phe Leu Leu Cys Ile Gly Trp Leu Ala 85 90 95 Leu Thr Asp Leu Val GlyGln Leu Leu Thr Thr Pro Val Val Ile Val 100 105 110 Val Tyr Leu Ser LysGln Arg Trp Glu His Ile Asp Pro Ser Gly Arg 115 120 125 Leu Cys Thr PhePhe Gly Leu Thr Met Thr Val Phe Gly Leu Ser Ser 130 135 140 Leu Phe IleAla Ser Ala Met Ala Val Glu Arg Ala Leu Ala Ile Arg 145 150 155 160 AlaPro His Trp Tyr Ala Ser His Met Lys Thr Arg Ala Thr Arg Ala 165 170 175Val Leu Leu Gly Val Trp Leu Ala Val Leu Ala Phe Ala Leu Leu Pro 180 185190 Val Leu Gly Val Gly Gln Tyr Thr Val Gln Trp Pro Gly Thr Trp Cys 195200 205 Phe Ile Ser Thr Gly Arg Gly Gly Asn Gly Thr Ser Ser Ser His Asn210 215 220 Trp Gly Asn Leu Phe Phe Ala Ser Ala Phe Ala Phe Leu Gly LeuLeu 225 230 235 240 Ala Leu Thr Val Thr Phe Ser Cys Asn Leu Ala Thr IleLys Ala Leu 245 250 255 Val Ser Arg Cys Arg Ala Lys Ala Thr Ala Ser GlnSer Ser Ala Gln 260 265 270 Trp Gly Arg Ile Thr Thr Glu Thr Ala Ile GlnLeu Met Gly Ile Met 275 280 285 Cys Val Leu Ser Val Cys Trp Ser Pro LeuLeu Ile Met Met Leu Lys 290 295 300 Met Ile Phe Asn Gln Thr Ser Val GluHis Cys Lys Thr His Thr Glu 305 310 315 320 Lys Gln Lys Glu Cys Asn PhePhe Leu Ile Ala Val Arg Leu Ala Ser 325 330 335 Leu Asn Gln Ile Leu AspPro Trp Val Tyr Leu Leu Leu Arg Lys Ile 340 345 350 Leu Leu Arg Lys PheCys Gln Met Arg Lys Arg Arg Leu Arg Glu Gln 355 360 365 Glu Met Gly ProAsp Gly Arg Cys Phe Cys His Ala Trp Arg Gln Val 370 375 380 Pro Arg ThrTrp Cys Ser Ser His Asp Arg Glu Pro Cys Ser Val Gln 385 390 395 400 LeuSer 35 1185 DNA Homo sapiens CDS (1)...(1179) cDNA 35 atg aag gag acccgg ggc tac gga ggg gat gcc ccc ttc tgc acc cgc 48 Met Lys Glu Thr ArgGly Tyr Gly Gly Asp Ala Pro Phe Cys Thr Arg 1 5 10 15 ctc aac cac tcctac aca ggc atg tgg gcg ccc gag cgt tcc gcc gag 96 Leu Asn His Ser TyrThr Gly Met Trp Ala Pro Glu Arg Ser Ala Glu 20 25 30 gcg cgg ggc aac ctcacg cgc cct cca ggg tct ggc gag gat tgc gga 144 Ala Arg Gly Asn Leu ThrArg Pro Pro Gly Ser Gly Glu Asp Cys Gly 35 40 45 tcg gtg tcc gtg gcc ttcccg atc acc atg ctg ctc act ggt ttc gtg 192 Ser Val Ser Val Ala Phe ProIle Thr Met Leu Leu Thr Gly Phe Val 50 55 60 ggc aac gca ctg gcc atg ctgctc gtg tcg cgc agc tac cgg cgc cgg 240 Gly Asn Ala Leu Ala Met Leu LeuVal Ser Arg Ser Tyr Arg Arg Arg 65 70 75 80 gag agc aag cgc aag aag tccttc ctg ctg tgc atc ggc tgg ctg gcg 288 Glu Ser Lys Arg Lys Lys Ser PheLeu Leu Cys Ile Gly Trp Leu Ala 85 90 95 ctc acc gac ctg gtc ggg cag cttctc acc acc ccg gtc gtc atc gtc 336 Leu Thr Asp Leu Val Gly Gln Leu LeuThr Thr Pro Val Val Ile Val 100 105 110 gtg tac ctg tcc aag cag cgt tgggag cac atc gac ccg tcg ggg cgg 384 Val Tyr Leu Ser Lys Gln Arg Trp GluHis Ile Asp Pro Ser Gly Arg 115 120 125 ctc tgc acc ttt ttc ggg ctg accatg act gtt ttc ggg ctc tcc tcg 432 Leu Cys Thr Phe Phe Gly Leu Thr MetThr Val Phe Gly Leu Ser Ser 130 135 140 ttg ttc atc gcc agc gcc atg gccgtc gag cgg gcg ctg gcc atc agg 480 Leu Phe Ile Ala Ser Ala Met Ala ValGlu Arg Ala Leu Ala Ile Arg 145 150 155 160 gcg ccg cac tgg tat gcg agccac atg aag acg cgt gcc acc cgc gct 528 Ala Pro His Trp Tyr Ala Ser HisMet Lys Thr Arg Ala Thr Arg Ala 165 170 175 gtg ctg ctc ggc gtg tgg ctggcc gtg ctc gcc ttc gcc ctg ctg ccg 576 Val Leu Leu Gly Val Trp Leu AlaVal Leu Ala Phe Ala Leu Leu Pro 180 185 190 gtg ctg ggc gtg ggc cag tacacc gtc cag tgg ccc ggg acg tgg tgc 624 Val Leu Gly Val Gly Gln Tyr ThrVal Gln Trp Pro Gly Thr Trp Cys 195 200 205 ttc atc agc acc ggg cga gggggc aac ggg act agc tct tcg cat aac 672 Phe Ile Ser Thr Gly Arg Gly GlyAsn Gly Thr Ser Ser Ser His Asn 210 215 220 tgg ggc aac ctt ttc ttc gcctct gcc ttt gcc ttc ctg ggg ctc ttg 720 Trp Gly Asn Leu Phe Phe Ala SerAla Phe Ala Phe Leu Gly Leu Leu 225 230 235 240 gcg ctg aca gtc acc ttttcc tgc aac ctg gcc acc att aag gcc ctg 768 Ala Leu Thr Val Thr Phe SerCys Asn Leu Ala Thr Ile Lys Ala Leu 245 250 255 gtg tcc cgc tgc cgg gccaag gcc acg gca tct cag tcc agt gcc cag 816 Val Ser Arg Cys Arg Ala LysAla Thr Ala Ser Gln Ser Ser Ala Gln 260 265 270 tgg ggc cgc atc acg accgag acg gcc att cag ctt atg ggg atc atg 864 Trp Gly Arg Ile Thr Thr GluThr Ala Ile Gln Leu Met Gly Ile Met 275 280 285 tgc gtg ctg tcg gtc tgctgg tct ccg ctc ctg ata atg atg ttg aaa 912 Cys Val Leu Ser Val Cys TrpSer Pro Leu Leu Ile Met Met Leu Lys 290 295 300 atg atc ttc aat cag acatca gtt gag cac tgc aag aca cac acg gag 960 Met Ile Phe Asn Gln Thr SerVal Glu His Cys Lys Thr His Thr Glu 305 310 315 320 aag cag aaa gaa tgcaac ttc ttc tta ata gct gtt cgc ctg gct tca 1008 Lys Gln Lys Glu Cys AsnPhe Phe Leu Ile Ala Val Arg Leu Ala Ser 325 330 335 ctg aac cag atc ttggat cct tgg gtt tac ctg ctg tta aga aag atc 1056 Leu Asn Gln Ile Leu AspPro Trp Val Tyr Leu Leu Leu Arg Lys Ile 340 345 350 ctt ctt cga aag ttttgc cag gag atg ggg cct gat gga agg tgt ttt 1104 Leu Leu Arg Lys Phe CysGln Glu Met Gly Pro Asp Gly Arg Cys Phe 355 360 365 tgt cat gca tgg aggcag gtc ccc agg act tgg tgc agt tct cat gat 1152 Cys His Ala Trp Arg GlnVal Pro Arg Thr Trp Cys Ser Ser His Asp 370 375 380 aga gaa ccc tgc agtgtc cag cta agc tgatga 1185 Arg Glu Pro Cys Ser Val Gln Leu Ser 385 39036 393 PRT Homo sapiens 36 Met Lys Glu Thr Arg Gly Tyr Gly Gly Asp AlaPro Phe Cys Thr Arg 1 5 10 15 Leu Asn His Ser Tyr Thr Gly Met Trp AlaPro Glu Arg Ser Ala Glu 20 25 30 Ala Arg Gly Asn Leu Thr Arg Pro Pro GlySer Gly Glu Asp Cys Gly 35 40 45 Ser Val Ser Val Ala Phe Pro Ile Thr MetLeu Leu Thr Gly Phe Val 50 55 60 Gly Asn Ala Leu Ala Met Leu Leu Val SerArg Ser Tyr Arg Arg Arg 65 70 75 80 Glu Ser Lys Arg Lys Lys Ser Phe LeuLeu Cys Ile Gly Trp Leu Ala 85 90 95 Leu Thr Asp Leu Val Gly Gln Leu LeuThr Thr Pro Val Val Ile Val 100 105 110 Val Tyr Leu Ser Lys Gln Arg TrpGlu His Ile Asp Pro Ser Gly Arg 115 120 125 Leu Cys Thr Phe Phe Gly LeuThr Met Thr Val Phe Gly Leu Ser Ser 130 135 140 Leu Phe Ile Ala Ser AlaMet Ala Val Glu Arg Ala Leu Ala Ile Arg 145 150 155 160 Ala Pro His TrpTyr Ala Ser His Met Lys Thr Arg Ala Thr Arg Ala 165 170 175 Val Leu LeuGly Val Trp Leu Ala Val Leu Ala Phe Ala Leu Leu Pro 180 185 190 Val LeuGly Val Gly Gln Tyr Thr Val Gln Trp Pro Gly Thr Trp Cys 195 200 205 PheIle Ser Thr Gly Arg Gly Gly Asn Gly Thr Ser Ser Ser His Asn 210 215 220Trp Gly Asn Leu Phe Phe Ala Ser Ala Phe Ala Phe Leu Gly Leu Leu 225 230235 240 Ala Leu Thr Val Thr Phe Ser Cys Asn Leu Ala Thr Ile Lys Ala Leu245 250 255 Val Ser Arg Cys Arg Ala Lys Ala Thr Ala Ser Gln Ser Ser AlaGln 260 265 270 Trp Gly Arg Ile Thr Thr Glu Thr Ala Ile Gln Leu Met GlyIle Met 275 280 285 Cys Val Leu Ser Val Cys Trp Ser Pro Leu Leu Ile MetMet Leu Lys 290 295 300 Met Ile Phe Asn Gln Thr Ser Val Glu His Cys LysThr His Thr Glu 305 310 315 320 Lys Gln Lys Glu Cys Asn Phe Phe Leu IleAla Val Arg Leu Ala Ser 325 330 335 Leu Asn Gln Ile Leu Asp Pro Trp ValTyr Leu Leu Leu Arg Lys Ile 340 345 350 Leu Leu Arg Lys Phe Cys Gln GluMet Gly Pro Asp Gly Arg Cys Phe 355 360 365 Cys His Ala Trp Arg Gln ValPro Arg Thr Trp Cys Ser Ser His Asp 370 375 380 Arg Glu Pro Cys Ser ValGln Leu Ser 385 390

What is claimed is:
 1. An isolated nucleic acid molecule comprising aprostaglandin E receptor subtype EP3 gene, or a fragment or variantthereof, wherein the gene comprises exon 4 (SEQ ID NO: 5) or 5 (SEQ IDNO: 6), or both exon 4 and exon
 5. 2. The isolated nucleic acid moleculeof claim 1, wherein the prostaglandin E receptor subtype EP3 gene hasthe nucleotide sequence of SEQ ID NO:1.
 3. A nucleic acid encoding apolypeptide having an amino acid sequence selected from the groupconsisting of SEQ ID NO: 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, and 36,wherein the nucleic acid comprises all or a portion of exon 4 (SEQ IDNO: 5) or 5 (SEQ ID NO: 6).
 4. An isolated nucleic acid moleculecomprising a nucleotide sequence selected from the group consisting ofSEQ ID NO: 1 and the complement of SEQ ID NO:
 1. 5. An isolated nucleicacid molecule which hybridizes under high stringency conditions to anucleotide sequence selected from the group consisting of SEQ ID NO: 1and the complement of SEQ ID NO:
 1. 6. An isolated nucleic acid moleculewhich hybridizes under high stringency conditions to a nucleotidesequence encoding an amino acid sequence selected from the groupconsisting of: SEQ ID NO: 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, and36, wherein the nucleic acid comprises all or a portion of exon 4 (SEQID NO: 5) or exon 5 (SEQ ID NO:6).
 7. A method for assaying the presenceof a first nucleic acid molecule in a sample, comprising contacting saidsample with a second nucleic acid molecule comprising a nucleotidesequence selected from the group consisting of SEQ ID NO: 1 and thecomplement of SEQ ID NO: 1, under high stringency conditions.
 8. Avector comprising an isolated nucleic acid molecule selected from thegroup consisting of: SEQ ID NO: 1, the complement of SEQ ID NO: 1, anucleic acid encoding SEQ ID NO: 16, a nucleic acid encoding SEQ ID NO:18, a nucleic acid encoding SEQ ID NO: 20, a nucleic acid encoding SEQID NO: 22, a nucleic acid encoding SEQ ID NO: 24, a nucleic acidencoding SEQ ID NO: 26, a nucleic acid encoding SEQ ID NO: 28, a nucleicacid encoding SEQ ID NO: 30, a nucleic acid encoding SEQ ID NO: 32, anucleic acid encoding SEQ ID NO: 34, and a nucleic acid encoding SEQ IDNO: 36, operatively linked to a regulatory sequence.
 9. A recombinanthost cell comprising the vector of claim
 8. 10. A method for producing apolypeptide encoded by an isolated nucleic acid molecule, comprisingculturing the recombinant host cell of claim 9 under conditions suitablefor expression of said nucleic acid molecule.
 11. An isolatedpolypeptide encoded by the isolated nucleic acid of claim
 1. 12. Theisolated polypeptide of claim 11, wherein the polypeptide comprises asequence selected from the group consisting of SEQ ID NO: 18 and SEQ IDNO:
 20. 13. An isolated polypeptide comprising an amino acid sequencewhich is greater than about 90 percent identical to an amino acidsequence selected from the group consisting of: SEQ ID NO: 18 and SEQ IDNO:
 20. 14. A fusion protein comprising an isolated polypeptide of claim11.
 15. An antibody, or an antigen-binding fragment thereof, whichselectively binds to the polypeptide of claim
 11. 16. A method forassaying the presence of a polypeptide encoded by an isolated nucleicacid molecule according to claim 1 in a sample, comprising contactingsaid sample with an antibody which specifically binds to the encodedpolypeptide.
 17. A method of diagnosing a susceptibility to peripheralarterial occlusive disease in an individual, comprising detecting apolymorphism in prostaglandin E receptor subtype EP3 gene, wherein thepresence of the polymorphism in the gene is indicative of asusceptibility to peripheral arterial occlusive disease.
 18. A method ofdiagnosing a susceptibility to peripheral arterial occlusive disease,comprising detecting an alteration in the expression or composition of apolypeptide encoded by prostaglandin E receptor subtype EP3 gene in atest sample, in comparison with the expression or composition of apolypeptide encoded by prostaglandin E receptor subtype EP3 gene in acontrol sample, wherein the presence of an alteration in expression orcomposition of the polypeptide in the test sample is indicative of asusceptibility to peripheral arterial occlusive disease.
 19. The methodof claim 18, wherein the alteration in the expression or composition ofa polypeptide encoded by prostaglandin E receptor subtype EP3 genecomprises expression of an isoform in a test sample that differs from anisoform expressed in a control sample.
 20. A method of identifying anagent which alters activity of a polypeptide of claim 11, comprising: a)contacting the polypeptide or a derivative or fragment thereof, with anagent to be tested; b) assessing the level of activity of thepolypeptide or derivative or fragment thereof; and c) comparing thelevel of activity with a level of activity of the polypeptide or activederivative or fragment thereof in the absence of the agent, wherein ifthe level of activity of the polypeptide or derivative or fragmentthereof in the presence of the agent differs, by an amount that isstatistically significant, from the level in the absence of the agent,then the agent is an agent that alters activity of the polypeptide. 21.An agent which alters activity of a polypeptide encoded prostaglandin Ereceptor subtype EP3 gene, identifiable according to the method of claim21.
 22. An agent which alters activity of a polypeptide encoded byprostaglandin E receptor subtype EP3 gene, wherein the agent is selectedfrom the group consisting of: a prostaglandin E receptor subtype EP3gene receptor; a prostaglandin E receptor subtype EP3 gene bindingagent; a peptidomimetic; a fusion protein; a prodrug; an antibody; and aribozyme.
 23. A method of altering activity of a polypeptide encoded byprostaglandin E receptor subtype EP3 gene, comprising contacting thepolypeptide with an agent of claim
 22. 24. A method of identifying anagent which alters interaction of the polypeptide of claim 11 with aprostaglandin E receptor subtype EP3 gene binding agent, comprising: a)contacting the polypeptide or a derivative or fragment thereof, thebinding agent and with an agent to be tested; b) assessing theinteraction of the polypeptide or derivative or fragment thereof withthe binding agent; and c) comparing the level of interaction with alevel of interaction of the polypeptide or derivative or fragmentthereof with the binding agent in the absence of the agent, wherein ifthe level of interaction of the polypeptide or derivative or fragmentthereof in the presence of the agent differs, by an amount that isstatistically significant, from the level of interaction in the absenceof the agent, then the agent is an agent that alters interaction of thepolypeptide with the binding agent.
 25. An agent which altersinteraction of a prostaglandin E receptor subtype EP3 gene polypeptidewith a prostaglandin E receptor subtype EP3 gene binding agent,identifiable according to the method of claim
 24. 26. An agent whichalters interaction of a prostaglandin E receptor subtype EP3 genepolypeptide with a first prostaglandin E receptor subtype EP3 genebinding agent, selected from the group consisting of: a prostaglandin Ereceptor subtype EP3 gene receptor; a prostaglandin E receptor subtypeEP3 gene binding agent; a peptidomimetic; a fusion protein; a prodrug;an antibody; and a ribozyme.
 27. A method of altering interaction of aprostaglandin E receptor subtype EP3 gene polypeptide with aprostaglandin E receptor subtype EP3 gene binding agent, comprisingcontacting the prostaglandin E receptor subtype EP3 gene polypeptideand/or the prostaglandin E receptor subtype EP3 gene binding agent withan agent of claim
 26. 28. A method of identifying an agent which altersexpression of prostaglandin E receptor subtype EP3 gene, comprising thesteps of: a) contacting a solution containing a nucleic acid of claim 1or a derivative or fragment thereof with an agent to be tested; b)assessing the level of expression of the nucleic acid, derivative orfragment; and c) comparing the level of expression with a level ofexpression of the nucleic acid, derivative or fragment in the absence ofthe agent, wherein if the level of expression of the nucleotide,derivative or fragment in the presence of the agent differs, by anamount that is statistically significant, from the expression in theabsence of the agent, then the agent is an agent that alters expressionof prostaglandin E receptor subtype EP3 gene.
 29. An agent which altersexpression of prostaglandin E receptor subtype EP3 gene, identifiableaccording to the method of claim
 28. 30. A method of identifying anagent which alters expression of prostaglandin E receptor subtype EP3gene, comprising the steps of: a) contacting a solution containing anucleic acid comprising the promoter region of prostaglandin E receptorsubtype EP3 gene operably linked to a reporter gene, with an agent to betested; b) assessing the level of expression of the reporter gene; andc) comparing the level of expression with a level of expression of thereporter gene in the absence of the agent, wherein if the level ofexpression of the reporter gene in the presence of the agent differs, byan amount that is statistically significant, from the level ofexpression in the absence of the agent, then the agent is an agent thatalters expression of prostaglandin E receptor subtype EP3 gene.
 31. Anagent which alters expression of prostaglandin E receptor subtype EP3gene, identifiable according to the method of claim
 32. 32. A method ofidentifying an agent which alters expression of prostaglandin E receptorsubtype EP3 gene, comprising the steps of: a) contacting a solutioncontaining a nucleic acid of claim 1 or a derivative or fragment thereofwith an agent to be tested; b) assessing expression of the nucleic acid,derivative or fragment; and c) comparing expression with expression ofthe nucleic acid, derivative or fragment in the absence of the agent,wherein if expression of the nucleotide, derivative or fragment in thepresence of the agent differs, by an amount that is statisticallysignificant, from the expression in the absence of the agent, then theagent is an agent that alters expression of prostaglandin E receptorsubtype EP3 gene.
 33. The method of claim 32, wherein the expression ofthe nucleotide, derivative or fragment in the presence of the agentcomprises expression of one or more splicing variant(s) that differ inkind or in quantity from the expression of one or more splicingvariant(s) the absence of the agent.
 34. An agent which altersexpression of prostaglandin E receptor subtype EP3 gene, identifiableaccording to the method of claim
 32. 35. An agent which altersexpression of prostaglandin E receptor subtype EP3 gene, selected fromthe group consisting of: antisense nucleic acid to prostaglandin Ereceptor subtype EP3 gene; a prostaglandin E receptor subtype EP3 genepolypeptide; a prostaglandin E receptor subtype EP3 gene receptor; aprostaglandin E receptor subtype EP3 gene binding agent; apeptidomimetic; a fusion protein; a prodrug thereof; an antibody; and aribozyme.
 36. A method of altering expression of prostaglandin Ereceptor subtype EP3 gene, comprising contacting a cell containingprostaglandin E receptor subtype EP3 gene with an agent of claim
 35. 37.A method of identifying a polypeptide which interacts with aprostaglandin E receptor subtype EP3 gene polypeptide, comprisingemploying a two yeast hybrid system using a first vector which comprisesa nucleic acid encoding a DNA binding domain and a prostaglandin Ereceptor subtype EP3 gene polypeptide, splicing variant, or fragment orderivative thereof, and a second vector which comprises a nucleic acidencoding a transcription activation domain and a nucleic acid encoding atest polypeptide, wherein if transcriptional activation occurs in thetwo yeast hybrid system, the test polypeptide is a polypeptide whichinteracts with a prostaglandin E receptor subtype EP3 gene polypeptide.38. A prostaglandin E receptor subtype EP3 gene therapeutic agentselected from the group consisting of: a prostaglandin E receptorsubtype EP3 gene or fragment or derivative thereof; a polypeptideencoded by prostaglandin E receptor subtype EP3 gene; a prostaglandin Ereceptor subtype EP3 gene receptor; a prostaglandin E receptor subtypeEP3 gene binding agent; a peptidomimetic; a fusion protein; a prodrug;an antibody; an agent that alters prostaglandin E receptor subtype EP3gene expression; an agent that alters activity of a polypeptide encodedby prostaglandin E receptor subtype EP3 gene; an agent that altersposttranscriptional processing of a polypeptide encoded by prostaglandinE receptor subtype EP3 gene; an agent that alters interaction of aprostaglandin E receptor subtype EP3 gene with a prostaglandin Ereceptor subtype EP3 gene binding agent; an agent that alterstranscription of splicing variants encoded by prostaglandin E receptorsubtype EP3 gene; and a ribozyme.
 39. A pharmaceutical compositioncomprising a prostaglandin E receptor subtype EP3 gene therapeutic agentof claim
 38. 40. The pharmaceutical composition of claim 39, wherein theprostaglandin E receptor subtype EP3 gene therapeutic agent is anisolated nucleic acid molecule comprising a prostaglandin E receptorsubtype EP3 gene or fragment or derivative thereof.
 41. Thepharmaceutical composition of claim 39, wherein the prostaglandin Ereceptor subtype EP3 gene therapeutic agent is a polypeptide encoded bythe prostaglandin E receptor subtype EP3 gene.
 42. A method of treatingperipheral arterial occlusive disease in an individual, comprisingadministering a prostaglandin E receptor subtype EP3 gene therapeuticagent to the individual, in a therapeutically effective amount.
 43. Themethod of claim 42, wherein the prostaglandin E receptor subtype EP3gene therapeutic agent is a prostaglandin E receptor subtype EP3 geneagonist.
 44. The method of claim 43 wherein the prostaglandin E receptorsubtype EP3 gene therapeutic agent is a prostaglandin E receptor subtypeEP3 gene antagonist.
 45. A transgenic animal comprising a nucleic acidselected from the group consisting of: an exogenous prostaglandin Ereceptor subtype EP3 gene and a nucleic acid encoding a prostaglandin Ereceptor subtype EP3 gene polypeptide.
 46. A method for assaying asample for the presence of a prostaglandin E receptor subtype EP3 genenucleic acid, comprising: a) contacting said sample with a nucleic acidcomprising a contiguous nucleotide sequence which is at least partiallycomplementary to a part of the sequence of said prostaglandin E receptorsubtype EP3 gene nucleic acid under conditions appropriate forhybridization, and b) assessing whether hybridization has occurredbetween a prostaglandin E receptor subtype EP3 gene nucleic acid andsaid nucleic acid comprising a contiguous nucleotide sequence which isat least partially complementary to a part of the sequence of saidprostaglandin E receptor subtype EP3 gene nucleic acid.
 47. The methodof claim 46, wherein said nucleic acid comprising a contiguousnucleotide sequence is completely complementary to a part of thesequence of said prostaglandin E receptor subtype EP3 gene nucleic acid.48. The method of claim 46, comprising amplification of at least part ofsaid prostaglandin E receptor subtype EP3 gene nucleic acid.
 49. Themethod of claim 47, wherein said contiguous nucleotide sequence is 100or fewer nucleotides in length and is either: a) at least 80% identicalto a contiguous sequence of nucleotides in SEQ ID NO: 1; b) at least 80%identical to the complement of a contiguous sequence of nucleotides inSEQ ID NO: 1; or c) capable of selectively hybridizing to saidprostaglandin E receptor subtype EP3 gene nucleic acid.
 50. A reagentfor assaying a sample for the presence of a prostaglandin E receptorsubtype EP3 gene nucleic acid, said reagent comprising a nucleic acidcomprising a contiguous nucleotide sequence which is at least partiallycomplementary to a part of the nucleotide sequence of said prostaglandinE receptor subtype EP3 gene nucleic acid.
 51. The reagent of claim 50,wherein the nucleic acid comprises a contiguous nucleotide sequencewhich is completely complementary to a part of the nucleotide sequenceof said prostaglandin E receptor subtype EP3 gene nucleic acid.
 52. Areagent kit for assaying a sample for the presence of a prostaglandin Ereceptor subtype EP3 gene nucleic acid, comprising in separatecontainers: a) one or more labeled nucleic acids comprising a contiguousnucleotide sequence which is at least partially complementary to a partof the nucleotide sequence of said prostaglandin E receptor subtype EP3gene nucleic acid, and b) reagents for detection of said label.
 53. Thereagent kit of claim 52, wherein the labeled nucleic acid comprises acontiguous nucleotide sequences which is completely complementary to apart of the nucleotide sequence of said prostaglandin E receptor subtypeEP3 gene nucleic acid.
 54. A reagent kit for assaying a sample for thepresence of a prostaglandin E receptor subtype EP3 gene nucleic acid,comprising one or more nucleic acids comprising a contiguous nucleotidesequence which is at least partially complementary to a part of thenucleotide sequence of said prostaglandin E receptor subtype EP3 genenucleic acid, and which is capable of acting as a primer for saidprostaglandin E receptor subtype EP3 gene nucleic acid when maintainedunder conditions for primer extension.